Strain Fitness in Pseudomonas putida KT2440 around PP_2454

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
2,799,794	+		PP_2453	0.54	-0.1
2,799,794	+		PP_2453	0.54	+0.0
2,799,878	-		PP_2453	0.62	-0.6
2,800,045	+		PP_2453	0.77	-0.1
2,800,045	+		PP_2453	0.77	+0.0
2,800,045	+		PP_2453	0.77	+0.1
2,800,045	+		PP_2453	0.77	+0.1
2,800,045	+				+0.1
2,800,046	-		PP_2453	0.78	-0.2
2,800,046	-		PP_2453	0.78	-0.1
2,800,046	-		PP_2453	0.78	+0.3
2,800,046	-		PP_2453	0.78	+0.0
2,800,046	-		PP_2453	0.78	-0.1
2,800,046	-		PP_2453	0.78	-0.2
2,800,115	+		PP_2453	0.84	+0.0
2,800,115	+		PP_2453	0.84	-0.3
2,800,116	-		PP_2453	0.84	+0.2
2,800,116	-		PP_2453	0.84	-0.2
2,800,310	+				-0.0
2,800,311	-				-0.3
2,800,318	+				+0.3
2,800,318	+				-0.0
2,800,319	-				-0.4
2,800,322	+				-0.3
2,800,340	+				+0.0
2,800,342	+				-0.1
2,800,342	+				-0.4
2,800,412	+				+0.3
2,800,412	+				-0.0
2,800,413	-				+0.2
2,800,413	-				-0.5
2,800,413	-				-0.5
2,800,476	+				-0.5
2,800,490	+				+1.1
2,800,490	+				-0.4
2,800,491	-				+0.2
2,800,529	-				-0.0
2,800,597	+				-0.3
2,800,597	+				-0.0
2,800,597	+				-0.4
2,800,598	-				-0.1
2,800,598	-				-0.6
2,800,598	-				+0.0
2,800,622	-				+0.5
2,801,052	+		PP_2454	0.41	-0.6
2,801,123	+		PP_2454	0.48	-0.2
2,801,123	+		PP_2454	0.48	-0.6
2,801,167	+		PP_2454	0.53	-0.6
2,801,168	-		PP_2454	0.53	-2.0
2,801,322	-		PP_2454	0.69	+0.4
2,801,513	+		PP_2454	0.89	-0.7
2,801,513	+		PP_2454	0.89	-0.7
2,801,514	-		PP_2454	0.89	+0.4
2,801,514	-		PP_2454	0.89	+0.0
2,801,514	-		PP_2454	0.89	+0.1
2,801,692	+				-0.1
2,801,692	+				+0.0
2,801,693	-				+0.4
2,801,782	-		PP_2455	0.10	-0.7
2,801,782	-		PP_2455	0.10	+0.9
2,801,796	+		PP_2455	0.11	+0.7
2,802,235	+		PP_2455	0.39	+0.2
2,802,236	-		PP_2455	0.39	+0.1
2,802,236	-		PP_2455	0.39	-0.3
2,802,236	-		PP_2455	0.39	+0.8
2,802,237	+		PP_2455	0.39	+0.2
2,802,238	-		PP_2455	0.39	-0.0
2,802,332	-		PP_2455	0.45	-0.2
2,802,332	-				+0.3
2,802,384	+		PP_2455	0.49	-0.8
2,802,384	+		PP_2455	0.49	-0.7
2,802,385	-		PP_2455	0.49	+0.4
2,802,547	+		PP_2455	0.59	-0.3
2,802,548	-		PP_2455	0.59	-0.4
2,802,548	-		PP_2455	0.59	-0.3