Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0500 and PP_0501 overlap by 8 nucleotides PP_0501 and PP_0502 are separated by 30 nucleotides PP_0502 and PP_0503 are separated by 102 nucleotides
PP_0500: PP_0500 - dTDP-4-rhamnose reductase-related protein, at 589,877 to 590,761
_0500
PP_0501: PP_0501 - NAD-dependent epimerase/dehydratase family protein, at 590,754 to 591,686
_0501
PP_0502: PP_0502 - conserved protein of unknown function, at 591,717 to 592,160
_0502
PP_0503: PP_0503 - Major facilitator family transporter, at 592,263 to 593,468
_0503
Position (kb) 591
592
593 Strain fitness (log2 ratio) -1
0
1
2 at 591.759 kb on + strand at 591.759 kb on + strand at 591.759 kb on + strand at 591.759 kb on + strand at 591.759 kb on + strand at 591.760 kb on - strand at 591.760 kb on - strand at 591.760 kb on - strand at 591.760 kb on - strand at 591.793 kb on - strand, within PP_0502 at 591.928 kb on + strand, within PP_0502 at 591.929 kb on - strand, within PP_0502 at 591.936 kb on - strand, within PP_0502 at 591.937 kb on + strand, within PP_0502 at 591.937 kb on + strand, within PP_0502 at 591.938 kb on - strand, within PP_0502 at 591.997 kb on - strand, within PP_0502 at 592.111 kb on - strand, within PP_0502 at 592.111 kb on - strand, within PP_0502 at 592.211 kb on - strand at 592.214 kb on + strand at 592.215 kb on - strand at 592.289 kb on - strand at 592.335 kb on + strand at 592.335 kb on + strand at 592.336 kb on - strand at 592.336 kb on - strand at 592.429 kb on - strand, within PP_0503 at 592.555 kb on + strand, within PP_0503 at 592.555 kb on + strand, within PP_0503 at 592.556 kb on - strand, within PP_0503 at 592.556 kb on - strand, within PP_0503 at 592.556 kb on - strand, within PP_0503 at 592.752 kb on + strand, within PP_0503 at 593.090 kb on + strand, within PP_0503 at 593.091 kb on - strand, within PP_0503 at 593.091 kb on - strand, within PP_0503
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1 remove 591,759 + -0.6 591,759 + -0.6 591,759 + +0.1 591,759 + -0.4 591,759 + -0.5 591,760 - -0.0 591,760 - -0.3 591,760 - -0.4 591,760 - -0.4 591,793 - PP_0502 0.17 -0.1 591,928 + PP_0502 0.48 -0.4 591,929 - PP_0502 0.48 -0.1 591,936 - PP_0502 0.49 -0.0 591,937 + PP_0502 0.50 -0.5 591,937 + PP_0502 0.50 -0.9 591,938 - PP_0502 0.50 -0.2 591,997 - PP_0502 0.63 -0.6 592,111 - PP_0502 0.89 -0.4 592,111 - PP_0502 0.89 -1.2 592,211 - +0.1 592,214 + +0.3 592,215 - -0.5 592,289 - +0.0 592,335 + +0.7 592,335 + -0.3 592,336 - -0.0 592,336 - -0.4 592,429 - PP_0503 0.14 -1.0 592,555 + PP_0503 0.24 -0.5 592,555 + PP_0503 0.24 +0.0 592,556 - PP_0503 0.24 -0.1 592,556 - PP_0503 0.24 -1.0 592,556 - PP_0503 0.24 -0.1 592,752 + PP_0503 0.41 +0.2 593,090 + PP_0503 0.69 +2.0 593,091 - PP_0503 0.69 -0.9 593,091 - PP_0503 0.69 -1.4
Or see this region's nucleotide sequence