Strain Fitness in Pseudomonas putida KT2440 around PP_0257

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0256 and PP_0257 are separated by 5 nucleotidesPP_0257 and PP_0258 are separated by 175 nucleotidesPP_0258 and PP_0259 are separated by 50 nucleotides PP_0256: PP_0256 - putative oxidoreductase, at 310,030 to 312,375 _0256 PP_0257: PP_0257 - protein sulfur transferase, at 312,381 to 313,211 _0257 PP_0258: PP_0258 - regulator of murein cross-linking, at 313,387 to 313,827 _0258 PP_0259: PP_0259 - purine nucleotidase, at 313,878 to 314,540 _0259 Position (kb) 312 313 314Strain fitness (log2 ratio) -1 0 1at 311.740 kb on - strand, within PP_0256at 311.828 kb on + strand, within PP_0256at 311.850 kb on - strand, within PP_0256at 311.850 kb on - strand, within PP_0256at 311.850 kb on - strand, within PP_0256at 311.855 kb on + strand, within PP_0256at 311.856 kb on - strand, within PP_0256at 311.856 kb on - strand, within PP_0256at 311.889 kb on - strand, within PP_0256at 311.889 kb on - strand, within PP_0256at 312.023 kb on + strand, within PP_0256at 312.105 kb on + strand, within PP_0256at 312.106 kb on - strand, within PP_0256at 312.250 kb on - strandat 312.250 kb on - strandat 312.279 kb on + strandat 312.280 kb on - strandat 312.314 kb on + strandat 312.314 kb on + strandat 312.323 kb on + strandat 312.324 kb on - strandat 312.382 kb on + strandat 312.383 kb on - strandat 312.404 kb on - strandat 312.404 kb on - strandat 312.437 kb on + strandat 312.925 kb on + strand, within PP_0257at 312.926 kb on - strand, within PP_0257at 312.926 kb on - strand, within PP_0257at 312.926 kb on - strand, within PP_0257at 313.069 kb on + strand, within PP_0257at 313.069 kb on + strand, within PP_0257at 313.069 kb on + strand, within PP_0257at 313.069 kb on + strand, within PP_0257at 313.070 kb on - strand, within PP_0257at 313.070 kb on - strand, within PP_0257at 313.139 kb on - strandat 313.144 kb on + strandat 313.144 kb on + strandat 313.145 kb on - strandat 313.145 kb on - strandat 313.241 kb on + strandat 313.289 kb on + strandat 313.290 kb on - strandat 313.299 kb on + strandat 313.300 kb on - strandat 313.300 kb on - strandat 313.336 kb on - strandat 313.472 kb on + strand, within PP_0258at 313.472 kb on + strand, within PP_0258at 313.473 kb on - strandat 313.527 kb on - strand, within PP_0258at 313.723 kb on + strand, within PP_0258at 313.724 kb on - strand, within PP_0258at 313.741 kb on + strand, within PP_0258at 313.742 kb on - strand, within PP_0258at 313.841 kb on + strandat 313.841 kb on + strandat 313.879 kb on + strandat 313.880 kb on - strandat 313.887 kb on + strandat 313.893 kb on + strandat 314.110 kb on + strand, within PP_0259at 314.110 kb on + strand, within PP_0259at 314.111 kb on - strand, within PP_0259at 314.140 kb on + strand, within PP_0259at 314.140 kb on + strand, within PP_0259at 314.140 kb on + strand, within PP_0259at 314.140 kb on + strand, within PP_0259at 314.141 kb on - strand, within PP_0259at 314.141 kb on - strand, within PP_0259at 314.141 kb on - strand, within PP_0259

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Per-strain Table

Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
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311,740 - PP_0256 0.73 -0.0
311,828 + PP_0256 0.77 +0.6
311,850 - PP_0256 0.78 +0.2
311,850 - PP_0256 0.78 -0.1
311,850 - PP_0256 0.78 -0.3
311,855 + PP_0256 0.78 -0.2
311,856 - PP_0256 0.78 +0.2
311,856 - PP_0256 0.78 -0.3
311,889 - PP_0256 0.79 -0.3
311,889 - PP_0256 0.79 +0.9
312,023 + PP_0256 0.85 +0.1
312,105 + PP_0256 0.88 +0.2
312,106 - PP_0256 0.88 -0.5
312,250 - +0.6
312,250 - -0.3
312,279 + +0.5
312,280 - +1.1
312,314 + -0.2
312,314 + -0.1
312,323 + +0.1
312,324 - -0.5
312,382 + -0.3
312,383 - -0.3
312,404 - -0.1
312,404 - -0.5
312,437 + +1.3
312,925 + PP_0257 0.65 -0.6
312,926 - PP_0257 0.66 -1.1
312,926 - PP_0257 0.66 +0.8
312,926 - PP_0257 0.66 -0.6
313,069 + PP_0257 0.83 -0.0
313,069 + PP_0257 0.83 -0.3
313,069 + PP_0257 0.83 -1.2
313,069 + PP_0257 0.83 -1.0
313,070 - PP_0257 0.83 -0.5
313,070 - PP_0257 0.83 +0.2
313,139 - -0.1
313,144 + -0.4
313,144 + -0.5
313,145 - +0.6
313,145 - +1.7
313,241 + -0.0
313,289 + -0.9
313,290 - -0.3
313,299 + -0.8
313,300 - -0.1
313,300 - -0.6
313,336 - +1.6
313,472 + PP_0258 0.19 -0.4
313,472 + PP_0258 0.19 -0.0
313,473 - +0.6
313,527 - PP_0258 0.32 -0.0
313,723 + PP_0258 0.76 -0.2
313,724 - PP_0258 0.76 +0.1
313,741 + PP_0258 0.80 +0.5
313,742 - PP_0258 0.80 +0.2
313,841 + -0.1
313,841 + -0.1
313,879 + -0.3
313,880 - +0.1
313,887 + +0.2
313,893 + -0.7
314,110 + PP_0259 0.35 +0.9
314,110 + PP_0259 0.35 -0.8
314,111 - PP_0259 0.35 +0.4
314,140 + PP_0259 0.40 +0.3
314,140 + PP_0259 0.40 +0.3
314,140 + PP_0259 0.40 -0.1
314,140 + PP_0259 0.40 +0.3
314,141 - PP_0259 0.40 +0.1
314,141 - PP_0259 0.40 -0.4
314,141 - PP_0259 0.40 -0.1

Or see this region's nucleotide sequence