Strain Fitness in Pseudomonas putida KT2440 around PP_4913

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 500 uM; timepoint 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_4912 and PP_4913 are separated by 13 nucleotidesPP_4913 and PP_4914 overlap by 4 nucleotides PP_4912: PP_4912 - DNA topoisomerase IV subunit A, at 5,583,473 to 5,585,731 _4912 PP_4913: PP_4913 - conserved protein of unknown function, at 5,585,745 to 5,586,263 _4913 PP_4914: PP_4914 - putative phytase-like protein, at 5,586,260 to 5,587,285 _4914 PP_4915: PP_4915 - DNA topoisomerase IV subunit B, at 5,587,243 to 5,589,147 _4915 Position (kb) 5585 5586 5587Strain fitness (log2 ratio) -1 0 1at 5585.747 kb on - strandat 5585.826 kb on + strand, within PP_4913at 5585.826 kb on + strand, within PP_4913at 5585.827 kb on - strand, within PP_4913at 5585.866 kb on - strand, within PP_4913at 5585.949 kb on + strand, within PP_4913at 5585.950 kb on - strand, within PP_4913at 5586.089 kb on + strand, within PP_4913at 5586.089 kb on + strand, within PP_4913at 5586.228 kb on + strandat 5586.229 kb on - strandat 5586.511 kb on + strand, within PP_4914at 5586.511 kb on + strand, within PP_4914at 5586.910 kb on + strand, within PP_4914at 5586.942 kb on + strand, within PP_4914at 5587.015 kb on + strand, within PP_4914at 5587.016 kb on - strand, within PP_4914at 5587.016 kb on - strand, within PP_4914at 5587.063 kb on + strand, within PP_4914at 5587.064 kb on - strand, within PP_4914at 5587.232 kb on + strandat 5587.233 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 500 uM; timepoint 1
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5,585,747 - -0.3
5,585,826 + PP_4913 0.16 -0.8
5,585,826 + PP_4913 0.16 -1.5
5,585,827 - PP_4913 0.16 +0.5
5,585,866 - PP_4913 0.23 -0.7
5,585,949 + PP_4913 0.39 -0.2
5,585,950 - PP_4913 0.39 -0.3
5,586,089 + PP_4913 0.66 -0.3
5,586,089 + PP_4913 0.66 -0.3
5,586,228 + +1.0
5,586,229 - +0.1
5,586,511 + PP_4914 0.24 -0.2
5,586,511 + PP_4914 0.24 +0.0
5,586,910 + PP_4914 0.63 -0.3
5,586,942 + PP_4914 0.66 +0.0
5,587,015 + PP_4914 0.74 -0.4
5,587,016 - PP_4914 0.74 -0.6
5,587,016 - PP_4914 0.74 -0.4
5,587,063 + PP_4914 0.78 -0.2
5,587,064 - PP_4914 0.78 -0.5
5,587,232 + -0.3
5,587,233 - -0.2

Or see this region's nucleotide sequence