Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 500 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2078 and PP_2079 are separated by 83 nucleotides PP_2079 and PP_2080 are separated by 170 nucleotides
PP_2078: PP_2078 - transcriptional activator protein LysR, at 2,365,565 to 2,366,470
_2078
PP_2079: PP_2079 - conserved protein of unknown function, at 2,366,554 to 2,367,012
_2079
PP_2080: PP_2080 - NAD-specific glutamate dehydrogenase, at 2,367,183 to 2,372,048
_2080
Position (kb)
2366
2367
2368 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2365.559 kb on - strand at 2365.784 kb on + strand, within PP_2078 at 2366.036 kb on + strand, within PP_2078 at 2366.138 kb on + strand, within PP_2078 at 2366.165 kb on - strand, within PP_2078 at 2366.377 kb on - strand, within PP_2078 at 2366.377 kb on - strand, within PP_2078 at 2366.377 kb on - strand, within PP_2078 at 2366.504 kb on - strand at 2366.545 kb on - strand at 2366.545 kb on - strand at 2366.657 kb on - strand, within PP_2079 at 2366.733 kb on + strand at 2366.734 kb on - strand, within PP_2079 at 2366.965 kb on + strand, within PP_2079 at 2366.965 kb on + strand, within PP_2079 at 2366.965 kb on + strand, within PP_2079 at 2366.965 kb on + strand, within PP_2079 at 2366.966 kb on - strand, within PP_2079 at 2366.966 kb on - strand, within PP_2079 at 2366.966 kb on - strand, within PP_2079 at 2367.016 kb on + strand at 2367.016 kb on + strand at 2367.016 kb on + strand at 2367.017 kb on - strand at 2367.088 kb on - strand at 2367.154 kb on + strand at 2367.176 kb on - strand at 2367.176 kb on - strand at 2367.420 kb on + strand at 2367.420 kb on + strand at 2367.421 kb on - strand at 2367.421 kb on - strand at 2367.432 kb on + strand at 2367.433 kb on - strand at 2367.643 kb on - strand at 2367.658 kb on - strand at 2367.658 kb on - strand at 2367.736 kb on + strand, within PP_2080 at 2367.736 kb on + strand, within PP_2080 at 2367.790 kb on + strand, within PP_2080 at 2367.895 kb on - strand, within PP_2080 at 2367.895 kb on - strand, within PP_2080
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 500 uM; timepoint 1 remove 2,365,559 - -0.5 2,365,784 + PP_2078 0.24 -0.5 2,366,036 + PP_2078 0.52 -0.2 2,366,138 + PP_2078 0.63 +0.4 2,366,165 - PP_2078 0.66 +0.2 2,366,377 - PP_2078 0.90 +0.3 2,366,377 - PP_2078 0.90 -0.4 2,366,377 - PP_2078 0.90 +0.1 2,366,504 - +0.3 2,366,545 - -1.1 2,366,545 - -0.3 2,366,657 - PP_2079 0.22 -0.6 2,366,733 + +1.4 2,366,734 - PP_2079 0.39 -0.6 2,366,965 + PP_2079 0.90 -0.9 2,366,965 + PP_2079 0.90 -0.1 2,366,965 + PP_2079 0.90 +0.1 2,366,965 + PP_2079 0.90 +0.0 2,366,966 - PP_2079 0.90 -0.5 2,366,966 - PP_2079 0.90 -0.4 2,366,966 - PP_2079 0.90 -0.2 2,367,016 + +3.0 2,367,016 + +1.0 2,367,016 + +0.5 2,367,017 - +0.2 2,367,088 - -0.6 2,367,154 + +0.2 2,367,176 - -0.3 2,367,176 - -0.4 2,367,420 + +0.1 2,367,420 + -0.3 2,367,421 - +0.0 2,367,421 - +0.0 2,367,432 + -0.2 2,367,433 - -0.1 2,367,643 - +0.2 2,367,658 - -0.5 2,367,658 - -0.8 2,367,736 + PP_2080 0.11 +0.5 2,367,736 + PP_2080 0.11 -0.4 2,367,790 + PP_2080 0.12 +0.7 2,367,895 - PP_2080 0.15 +0.2 2,367,895 - PP_2080 0.15 +0.0
Or see this region's nucleotide sequence