Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 500 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_t25 and PP_t26 are separated by 72 nucleotides PP_t26 and PP_t27 are separated by 51 nucleotides PP_t27 and PP_t28 are separated by 42 nucleotides PP_t28 and PP_t29 are separated by 61 nucleotides PP_t29 and PP_t30 are separated by 42 nucleotides PP_t30 and PP_1978 are separated by 170 nucleotides PP_1978 and PP_1979 are separated by 15 nucleotides
PP_t25: PP_t25 - tRNA-Ala, at 2,241,534 to 2,241,609
_t25
PP_t26: PP_t26 - tRNA-Glu, at 2,241,682 to 2,241,757
_t26
PP_t27: PP_t27 - tRNA-Ala, at 2,241,809 to 2,241,884
_t27
PP_t28: PP_t28 - tRNA-Glu, at 2,241,927 to 2,242,002
_t28
PP_t29: PP_t29 - tRNA-Ala, at 2,242,064 to 2,242,139
_t29
PP_t30: PP_t30 - tRNA-Glu, at 2,242,182 to 2,242,257
_t30
PP_1978: PP_1978 - Transcriptional regulator, TetR family, at 2,242,428 to 2,242,967
_1978
PP_1979: PP_1979 - putative Hydrolase, at 2,242,983 to 2,243,864
_1979
Position (kb)
2242
2243 Strain fitness (log2 ratio)
-2
-1
0
1 at 2241.762 kb on + strand at 2241.778 kb on + strand at 2241.874 kb on + strand, within PP_t27 at 2242.026 kb on + strand at 2242.072 kb on - strand, within PP_t29 at 2242.072 kb on - strand at 2242.072 kb on - strand, within PP_t29 at 2242.072 kb on - strand, within PP_t29 at 2242.129 kb on + strand, within PP_t29 at 2242.180 kb on - strand at 2242.284 kb on - strand at 2242.379 kb on - strand at 2242.560 kb on + strand, within PP_1978 at 2242.560 kb on + strand, within PP_1978 at 2242.560 kb on + strand, within PP_1978 at 2242.560 kb on + strand, within PP_1978 at 2242.561 kb on - strand, within PP_1978 at 2242.561 kb on - strand, within PP_1978 at 2242.589 kb on + strand, within PP_1978 at 2242.589 kb on + strand, within PP_1978 at 2242.589 kb on + strand, within PP_1978 at 2242.589 kb on + strand, within PP_1978 at 2242.590 kb on - strand, within PP_1978 at 2242.590 kb on - strand, within PP_1978 at 2242.590 kb on - strand, within PP_1978 at 2242.590 kb on - strand, within PP_1978 at 2242.695 kb on + strand, within PP_1978 at 2242.695 kb on + strand, within PP_1978 at 2242.696 kb on - strand, within PP_1978 at 2242.726 kb on + strand, within PP_1978 at 2242.726 kb on + strand, within PP_1978 at 2242.727 kb on - strand, within PP_1978 at 2242.988 kb on + strand at 2242.989 kb on - strand at 2242.989 kb on - strand at 2243.139 kb on - strand, within PP_1979 at 2243.139 kb on - strand, within PP_1979 at 2243.264 kb on + strand, within PP_1979 at 2243.265 kb on - strand, within PP_1979 at 2243.265 kb on - strand, within PP_1979 at 2243.798 kb on + strand at 2243.832 kb on - strand at 2243.886 kb on - strand at 2243.887 kb on + strand at 2243.887 kb on + strand at 2243.888 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 500 uM; timepoint 1 remove 2,241,762 + -0.7 2,241,778 + -2.2 2,241,874 + PP_t27 0.86 -0.3 2,242,026 + +0.6 2,242,072 - PP_t29 0.11 -0.3 2,242,072 - -1.0 2,242,072 - PP_t29 0.11 -0.0 2,242,072 - PP_t29 0.11 +0.4 2,242,129 + PP_t29 0.86 -0.7 2,242,180 - +1.2 2,242,284 - +0.4 2,242,379 - -0.1 2,242,560 + PP_1978 0.24 -0.4 2,242,560 + PP_1978 0.24 -0.2 2,242,560 + PP_1978 0.24 -0.7 2,242,560 + PP_1978 0.24 -0.7 2,242,561 - PP_1978 0.25 +0.4 2,242,561 - PP_1978 0.25 -0.3 2,242,589 + PP_1978 0.30 -0.2 2,242,589 + PP_1978 0.30 +0.3 2,242,589 + PP_1978 0.30 +0.4 2,242,589 + PP_1978 0.30 -0.5 2,242,590 - PP_1978 0.30 -1.5 2,242,590 - PP_1978 0.30 -0.1 2,242,590 - PP_1978 0.30 +0.0 2,242,590 - PP_1978 0.30 -0.5 2,242,695 + PP_1978 0.49 -0.8 2,242,695 + PP_1978 0.49 -1.0 2,242,696 - PP_1978 0.50 -0.5 2,242,726 + PP_1978 0.55 -1.5 2,242,726 + PP_1978 0.55 -1.2 2,242,727 - PP_1978 0.55 -0.0 2,242,988 + -0.6 2,242,989 - +0.5 2,242,989 - -0.3 2,243,139 - PP_1979 0.18 +0.0 2,243,139 - PP_1979 0.18 -0.9 2,243,264 + PP_1979 0.32 -0.2 2,243,265 - PP_1979 0.32 -1.1 2,243,265 - PP_1979 0.32 -0.3 2,243,798 + -0.1 2,243,832 - -0.2 2,243,886 - +0.2 2,243,887 + -0.1 2,243,887 + -0.7 2,243,888 - -0.2
Or see this region's nucleotide sequence