Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 500 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1613 and PP_1614 overlap by 4 nucleotides PP_1614 and PP_1615 are separated by 127 nucleotides PP_1615 and PP_1616 are separated by 104 nucleotides
PP_1613: PP_1613 - essential cell division protein, at 1,810,408 to 1,810,689
_1613
PP_1614: PP_1614 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 1,810,686 to 1,811,393
_1614
PP_1615: PP_1615 - Transcriptional regulator, LysR family, at 1,811,521 to 1,812,417
_1615
PP_1616: PP_1616 - formaldehyde dehydrogenase, glutathione-dependent, at 1,812,522 to 1,813,637
_1616
Position (kb)
1811
1812
1813 Strain fitness (log2 ratio)
-1
0
1 at 1811.435 kb on + strand at 1811.435 kb on + strand at 1811.435 kb on + strand at 1811.436 kb on - strand at 1811.473 kb on + strand at 1811.473 kb on + strand at 1811.474 kb on - strand at 1811.503 kb on + strand at 1811.503 kb on + strand at 1811.503 kb on + strand at 1811.535 kb on - strand at 1811.627 kb on - strand, within PP_1615 at 1811.699 kb on + strand, within PP_1615 at 1811.699 kb on + strand, within PP_1615 at 1811.699 kb on + strand, within PP_1615 at 1811.700 kb on - strand, within PP_1615 at 1811.700 kb on - strand, within PP_1615 at 1811.700 kb on - strand, within PP_1615 at 1811.700 kb on - strand, within PP_1615 at 1811.702 kb on + strand, within PP_1615 at 1811.703 kb on - strand, within PP_1615 at 1811.778 kb on + strand, within PP_1615 at 1811.779 kb on - strand, within PP_1615 at 1811.779 kb on - strand, within PP_1615 at 1811.779 kb on - strand, within PP_1615 at 1811.819 kb on - strand, within PP_1615 at 1811.891 kb on + strand, within PP_1615 at 1811.892 kb on - strand, within PP_1615 at 1811.903 kb on + strand, within PP_1615 at 1811.924 kb on + strand, within PP_1615 at 1811.924 kb on + strand, within PP_1615 at 1811.924 kb on + strand, within PP_1615 at 1811.924 kb on + strand, within PP_1615 at 1811.925 kb on - strand, within PP_1615 at 1811.925 kb on - strand, within PP_1615 at 1812.063 kb on - strand, within PP_1615 at 1812.461 kb on + strand at 1812.461 kb on + strand at 1812.660 kb on + strand, within PP_1616 at 1812.661 kb on - strand, within PP_1616 at 1812.661 kb on - strand, within PP_1616 at 1812.661 kb on - strand, within PP_1616 at 1812.662 kb on + strand, within PP_1616 at 1812.662 kb on + strand, within PP_1616 at 1812.662 kb on + strand, within PP_1616 at 1812.663 kb on - strand, within PP_1616 at 1812.690 kb on - strand, within PP_1616 at 1812.690 kb on - strand, within PP_1616 at 1812.690 kb on - strand, within PP_1616 at 1812.790 kb on - strand, within PP_1616 at 1812.790 kb on - strand, within PP_1616 at 1812.906 kb on + strand, within PP_1616 at 1812.930 kb on + strand, within PP_1616 at 1812.930 kb on + strand, within PP_1616 at 1812.930 kb on + strand, within PP_1616 at 1812.931 kb on - strand, within PP_1616 at 1812.931 kb on - strand, within PP_1616 at 1812.957 kb on + strand, within PP_1616 at 1813.091 kb on + strand, within PP_1616 at 1813.091 kb on + strand, within PP_1616 at 1813.092 kb on - strand, within PP_1616 at 1813.166 kb on + strand, within PP_1616 at 1813.248 kb on + strand, within PP_1616 at 1813.248 kb on + strand, within PP_1616 at 1813.249 kb on - strand, within PP_1616 at 1813.249 kb on - strand, within PP_1616
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 500 uM; timepoint 1 remove 1,811,435 + +0.0 1,811,435 + +0.3 1,811,435 + -0.3 1,811,436 - -0.1 1,811,473 + -0.2 1,811,473 + -0.2 1,811,474 - -0.1 1,811,503 + -0.6 1,811,503 + -1.3 1,811,503 + -0.3 1,811,535 - +0.1 1,811,627 - PP_1615 0.12 +0.7 1,811,699 + PP_1615 0.20 +0.0 1,811,699 + PP_1615 0.20 -0.1 1,811,699 + PP_1615 0.20 -0.5 1,811,700 - PP_1615 0.20 -0.3 1,811,700 - PP_1615 0.20 +0.1 1,811,700 - PP_1615 0.20 +0.6 1,811,700 - PP_1615 0.20 -0.2 1,811,702 + PP_1615 0.20 +0.1 1,811,703 - PP_1615 0.20 -0.6 1,811,778 + PP_1615 0.29 +0.0 1,811,779 - PP_1615 0.29 -0.5 1,811,779 - PP_1615 0.29 -0.4 1,811,779 - PP_1615 0.29 +0.1 1,811,819 - PP_1615 0.33 +0.4 1,811,891 + PP_1615 0.41 +0.5 1,811,892 - PP_1615 0.41 -0.4 1,811,903 + PP_1615 0.43 +0.5 1,811,924 + PP_1615 0.45 -0.0 1,811,924 + PP_1615 0.45 -1.0 1,811,924 + PP_1615 0.45 -0.4 1,811,924 + PP_1615 0.45 +0.4 1,811,925 - PP_1615 0.45 -0.9 1,811,925 - PP_1615 0.45 +0.4 1,812,063 - PP_1615 0.60 -0.3 1,812,461 + +0.4 1,812,461 + +0.0 1,812,660 + PP_1616 0.12 -0.7 1,812,661 - PP_1616 0.12 -0.0 1,812,661 - PP_1616 0.12 -0.5 1,812,661 - PP_1616 0.12 -0.1 1,812,662 + PP_1616 0.13 +0.0 1,812,662 + PP_1616 0.13 +0.1 1,812,662 + PP_1616 0.13 -0.0 1,812,663 - PP_1616 0.13 +0.1 1,812,690 - PP_1616 0.15 -0.1 1,812,690 - PP_1616 0.15 -0.1 1,812,690 - PP_1616 0.15 +0.2 1,812,790 - PP_1616 0.24 +0.1 1,812,790 - PP_1616 0.24 -0.0 1,812,906 + PP_1616 0.34 +1.0 1,812,930 + PP_1616 0.37 -0.7 1,812,930 + PP_1616 0.37 -1.0 1,812,930 + PP_1616 0.37 -0.3 1,812,931 - PP_1616 0.37 +0.2 1,812,931 - PP_1616 0.37 +0.4 1,812,957 + PP_1616 0.39 +0.2 1,813,091 + PP_1616 0.51 +0.5 1,813,091 + PP_1616 0.51 +0.5 1,813,092 - PP_1616 0.51 -0.0 1,813,166 + PP_1616 0.58 -0.0 1,813,248 + PP_1616 0.65 -0.4 1,813,248 + PP_1616 0.65 +0.0 1,813,249 - PP_1616 0.65 -0.2 1,813,249 - PP_1616 0.65 +0.6
Or see this region's nucleotide sequence