Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5278 and PP_5279 are separated by 196 nucleotides PP_5279 and PP_5729 are separated by 38 nucleotides PP_5729 and PP_5281 are separated by 66 nucleotides PP_5281 and PP_5282 are separated by 11 nucleotides
PP_5278: PP_5278 - 4-guanidinobutyraldehyde dehydrogenase, at 6,027,967 to 6,029,460
_5278
PP_5279: PP_5279 - conserved protein of unknown function, at 6,029,657 to 6,030,016
_5279
PP_5729: PP_5729 - conserved protein of unknown function, at 6,030,055 to 6,030,315
_5729
PP_5281: PP_5281 - 50S ribosomal protein L33, at 6,030,382 to 6,030,537
_5281
PP_5282: PP_5282 - 50S ribosomal protein L28, at 6,030,549 to 6,030,785
_5282
Position (kb)
6029
6030
6031 Strain fitness (log2 ratio)
-2
-1
0
1 at 6028.794 kb on - strand, within PP_5278 at 6028.794 kb on - strand, within PP_5278 at 6028.906 kb on - strand, within PP_5278 at 6029.045 kb on + strand, within PP_5278 at 6029.045 kb on + strand, within PP_5278 at 6029.046 kb on - strand, within PP_5278 at 6029.046 kb on - strand, within PP_5278 at 6029.046 kb on - strand, within PP_5278 at 6029.046 kb on - strand, within PP_5278 at 6029.461 kb on - strand at 6029.482 kb on - strand at 6029.482 kb on - strand at 6029.564 kb on - strand at 6029.632 kb on - strand at 6029.662 kb on + strand at 6029.662 kb on + strand at 6029.662 kb on + strand at 6029.662 kb on + strand at 6029.662 kb on + strand at 6029.662 kb on + strand at 6029.662 kb on + strand at 6029.662 kb on + strand at 6029.662 kb on + strand at 6029.663 kb on - strand at 6029.663 kb on - strand at 6029.663 kb on - strand at 6029.663 kb on - strand at 6029.663 kb on - strand at 6029.663 kb on - strand at 6029.763 kb on - strand, within PP_5279 at 6029.763 kb on - strand, within PP_5279 at 6029.806 kb on - strand, within PP_5279 at 6029.841 kb on - strand, within PP_5279 at 6029.855 kb on + strand, within PP_5279 at 6029.856 kb on - strand, within PP_5279 at 6029.856 kb on - strand, within PP_5279 at 6029.937 kb on + strand, within PP_5279 at 6029.938 kb on - strand, within PP_5279 at 6029.938 kb on - strand, within PP_5279 at 6029.993 kb on + strand at 6029.993 kb on + strand at 6029.994 kb on - strand at 6029.994 kb on - strand at 6029.994 kb on - strand at 6029.994 kb on - strand at 6029.994 kb on - strand at 6030.015 kb on - strand at 6030.026 kb on + strand at 6030.026 kb on + strand at 6030.026 kb on + strand at 6030.026 kb on + strand at 6030.026 kb on + strand at 6030.026 kb on + strand at 6030.027 kb on - strand at 6030.027 kb on - strand at 6030.027 kb on - strand at 6030.027 kb on - strand at 6030.027 kb on - strand at 6030.030 kb on + strand at 6030.030 kb on + strand at 6030.030 kb on + strand at 6030.030 kb on + strand at 6030.030 kb on + strand at 6030.030 kb on + strand at 6030.030 kb on + strand at 6030.030 kb on + strand at 6030.030 kb on + strand at 6030.030 kb on + strand at 6030.030 kb on + strand at 6030.031 kb on - strand at 6030.031 kb on - strand at 6030.031 kb on - strand at 6030.031 kb on - strand at 6030.031 kb on - strand at 6030.031 kb on - strand at 6030.031 kb on - strand at 6030.034 kb on + strand at 6030.034 kb on + strand at 6030.034 kb on + strand at 6030.035 kb on - strand at 6030.967 kb on - strand at 6030.967 kb on - strand at 6030.967 kb on - strand at 6030.978 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1 remove 6,028,794 - PP_5278 0.55 +0.1 6,028,794 - PP_5278 0.55 -0.5 6,028,906 - PP_5278 0.63 +0.5 6,029,045 + PP_5278 0.72 +0.3 6,029,045 + PP_5278 0.72 -0.2 6,029,046 - PP_5278 0.72 -0.2 6,029,046 - PP_5278 0.72 -0.2 6,029,046 - PP_5278 0.72 -0.8 6,029,046 - PP_5278 0.72 -0.2 6,029,461 - -0.3 6,029,482 - -0.1 6,029,482 - -0.0 6,029,564 - -0.2 6,029,632 - -0.4 6,029,662 + +0.4 6,029,662 + +0.0 6,029,662 + -0.2 6,029,662 + -0.6 6,029,662 + +0.5 6,029,662 + -1.1 6,029,662 + -0.6 6,029,662 + -0.8 6,029,662 + +0.3 6,029,663 - -0.2 6,029,663 - +0.3 6,029,663 - -0.5 6,029,663 - +0.4 6,029,663 - -0.3 6,029,663 - -0.9 6,029,763 - PP_5279 0.29 -2.0 6,029,763 - PP_5279 0.29 -1.4 6,029,806 - PP_5279 0.41 -1.0 6,029,841 - PP_5279 0.51 -1.1 6,029,855 + PP_5279 0.55 -0.3 6,029,856 - PP_5279 0.55 +0.0 6,029,856 - PP_5279 0.55 -0.3 6,029,937 + PP_5279 0.78 +0.6 6,029,938 - PP_5279 0.78 -0.3 6,029,938 - PP_5279 0.78 -1.2 6,029,993 + +0.1 6,029,993 + -0.2 6,029,994 - +0.3 6,029,994 - -0.7 6,029,994 - -0.4 6,029,994 - -0.5 6,029,994 - -1.0 6,030,015 - -0.4 6,030,026 + +0.4 6,030,026 + +0.7 6,030,026 + +0.3 6,030,026 + +0.5 6,030,026 + +0.0 6,030,026 + +0.0 6,030,027 - -0.8 6,030,027 - -0.1 6,030,027 - -0.3 6,030,027 - -0.3 6,030,027 - -0.6 6,030,030 + +0.6 6,030,030 + +0.2 6,030,030 + +0.3 6,030,030 + -0.8 6,030,030 + -0.0 6,030,030 + -0.7 6,030,030 + -0.0 6,030,030 + +0.1 6,030,030 + -0.3 6,030,030 + -0.1 6,030,030 + -0.1 6,030,031 - -1.4 6,030,031 - -0.4 6,030,031 - -1.1 6,030,031 - -1.3 6,030,031 - +0.4 6,030,031 - -0.3 6,030,031 - -0.6 6,030,034 + +0.1 6,030,034 + +0.4 6,030,034 + -0.1 6,030,035 - -2.4 6,030,967 - -0.9 6,030,967 - -0.3 6,030,967 - -0.6 6,030,978 - +0.4
Or see this region's nucleotide sequence