Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1935 and PP_5487 are separated by 154 nucleotides PP_5487 and PP_5488 are separated by 13 nucleotides PP_5488 and PP_5489 are separated by 16 nucleotides PP_5489 and PP_5490 are separated by 173 nucleotides
PP_1935: PP_1935 - Transcriptional regulator, Cro/CI family, at 2,181,727 to 2,182,035
_1935
PP_5487: PP_5487 - conserved protein of unknown function, at 2,182,190 to 2,182,582
_5487
PP_5488: PP_5488 - conserved protein of unknown function, at 2,182,596 to 2,183,255
_5488
PP_5489: PP_5489 - conserved protein of unknown function, at 2,183,272 to 2,183,769
_5489
PP_5490: PP_5490 - site-specific DNA recombinase, at 2,183,943 to 2,184,509
_5490
Position (kb)
2182
2183
2184 Strain fitness (log2 ratio)
-1
0
1 at 2181.610 kb on + strand at 2181.645 kb on + strand at 2181.646 kb on - strand at 2181.655 kb on + strand at 2181.656 kb on - strand at 2181.668 kb on - strand at 2181.678 kb on + strand at 2181.719 kb on + strand at 2181.729 kb on - strand at 2181.885 kb on - strand, within PP_1935 at 2181.911 kb on + strand, within PP_1935 at 2181.949 kb on - strand, within PP_1935 at 2182.069 kb on - strand at 2182.132 kb on - strand at 2182.186 kb on - strand at 2182.194 kb on + strand at 2182.195 kb on - strand at 2182.359 kb on + strand, within PP_5487 at 2182.408 kb on + strand, within PP_5487 at 2182.413 kb on - strand, within PP_5487 at 2182.446 kb on + strand, within PP_5487 at 2182.580 kb on + strand at 2182.698 kb on + strand, within PP_5488 at 2182.743 kb on + strand, within PP_5488 at 2182.743 kb on + strand, within PP_5488 at 2182.909 kb on - strand, within PP_5488 at 2182.938 kb on + strand, within PP_5488 at 2182.938 kb on + strand, within PP_5488 at 2182.940 kb on + strand, within PP_5488 at 2183.189 kb on + strand, within PP_5488 at 2183.191 kb on + strand at 2183.192 kb on - strand at 2183.192 kb on - strand at 2183.195 kb on + strand at 2183.253 kb on + strand at 2183.254 kb on - strand at 2183.258 kb on + strand at 2183.259 kb on - strand at 2183.507 kb on - strand, within PP_5489 at 2183.535 kb on + strand, within PP_5489 at 2183.536 kb on - strand, within PP_5489 at 2183.592 kb on + strand, within PP_5489 at 2183.597 kb on + strand, within PP_5489 at 2183.597 kb on + strand, within PP_5489 at 2183.743 kb on - strand at 2183.789 kb on - strand at 2183.828 kb on - strand at 2183.942 kb on + strand at 2183.992 kb on + strand at 2184.004 kb on + strand, within PP_5490 at 2184.167 kb on + strand, within PP_5490 at 2184.167 kb on + strand, within PP_5490 at 2184.168 kb on - strand, within PP_5490 at 2184.236 kb on + strand, within PP_5490 at 2184.237 kb on - strand, within PP_5490
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1 remove 2,181,610 + +0.1 2,181,645 + +0.2 2,181,646 - -0.2 2,181,655 + +0.3 2,181,656 - -0.2 2,181,668 - +0.0 2,181,678 + -1.1 2,181,719 + -0.2 2,181,729 - -0.2 2,181,885 - PP_1935 0.51 +0.5 2,181,911 + PP_1935 0.60 -0.1 2,181,949 - PP_1935 0.72 +0.1 2,182,069 - -0.1 2,182,132 - -0.3 2,182,186 - -0.0 2,182,194 + +0.6 2,182,195 - -0.0 2,182,359 + PP_5487 0.43 -0.2 2,182,408 + PP_5487 0.55 -0.4 2,182,413 - PP_5487 0.57 +0.4 2,182,446 + PP_5487 0.65 +0.4 2,182,580 + +0.2 2,182,698 + PP_5488 0.15 -0.1 2,182,743 + PP_5488 0.22 -0.6 2,182,743 + PP_5488 0.22 -0.5 2,182,909 - PP_5488 0.47 +0.3 2,182,938 + PP_5488 0.52 -0.4 2,182,938 + PP_5488 0.52 -1.1 2,182,940 + PP_5488 0.52 -0.7 2,183,189 + PP_5488 0.90 -0.0 2,183,191 + -0.1 2,183,192 - -0.2 2,183,192 - +0.5 2,183,195 + -0.2 2,183,253 + +0.1 2,183,254 - -1.3 2,183,258 + -0.1 2,183,259 - +0.1 2,183,507 - PP_5489 0.47 -0.1 2,183,535 + PP_5489 0.53 +0.1 2,183,536 - PP_5489 0.53 -0.4 2,183,592 + PP_5489 0.64 -0.6 2,183,597 + PP_5489 0.65 +0.0 2,183,597 + PP_5489 0.65 -0.6 2,183,743 - -0.6 2,183,789 - -0.3 2,183,828 - -0.0 2,183,942 + +0.5 2,183,992 + +0.4 2,184,004 + PP_5490 0.11 -1.7 2,184,167 + PP_5490 0.40 -1.1 2,184,167 + PP_5490 0.40 +0.0 2,184,168 - PP_5490 0.40 +0.4 2,184,236 + PP_5490 0.52 -0.1 2,184,237 - PP_5490 0.52 -0.6
Or see this region's nucleotide sequence