Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5242 and PP_5243 are separated by 88 nucleotides PP_5243 and PP_5244 are separated by 71 nucleotides PP_5244 and PP_5245 are separated by 138 nucleotides
PP_5242: PP_5242 - Sensor histidine kinase/GAF domain protein, at 5,979,356 to 5,984,404
_5242
PP_5243: PP_5243 - putative hydrolase, at 5,984,493 to 5,985,119
_5243
PP_5244: PP_5244 - Transcriptional regulator, AraC family, at 5,985,191 to 5,985,595
_5244
PP_5245: PP_5245 - Transcriptional regulator, AraC family, at 5,985,734 to 5,986,720
_5245
Position (kb) 5984
5985
5986 Strain fitness (log2 ratio) -1
0
1 at 5983.536 kb on - strand, within PP_5242 at 5983.623 kb on + strand, within PP_5242 at 5983.624 kb on - strand, within PP_5242 at 5983.641 kb on - strand, within PP_5242 at 5984.110 kb on + strand at 5984.164 kb on + strand at 5984.248 kb on - strand at 5984.263 kb on + strand at 5984.263 kb on + strand at 5984.264 kb on - strand at 5984.305 kb on + strand at 5984.305 kb on + strand at 5984.306 kb on - strand at 5984.403 kb on - strand at 5984.403 kb on - strand at 5984.439 kb on + strand at 5984.451 kb on + strand at 5984.481 kb on + strand at 5984.481 kb on + strand at 5984.482 kb on - strand at 5984.482 kb on - strand at 5984.489 kb on + strand at 5984.489 kb on + strand at 5984.490 kb on - strand at 5984.515 kb on + strand at 5984.516 kb on - strand at 5984.658 kb on + strand, within PP_5243 at 5984.658 kb on + strand, within PP_5243 at 5984.875 kb on + strand, within PP_5243 at 5984.875 kb on + strand, within PP_5243 at 5984.876 kb on - strand, within PP_5243 at 5985.103 kb on + strand at 5985.103 kb on + strand at 5985.103 kb on + strand at 5985.171 kb on + strand at 5985.171 kb on + strand at 5985.182 kb on - strand at 5985.193 kb on - strand at 5985.308 kb on - strand, within PP_5244 at 5985.308 kb on - strand, within PP_5244 at 5985.463 kb on + strand, within PP_5244 at 5985.463 kb on + strand, within PP_5244 at 5985.464 kb on - strand, within PP_5244 at 5985.549 kb on + strand, within PP_5244 at 5985.549 kb on + strand, within PP_5244 at 5985.549 kb on + strand, within PP_5244 at 5985.550 kb on - strand, within PP_5244 at 5985.630 kb on + strand at 5985.631 kb on - strand at 5985.670 kb on + strand at 5985.671 kb on - strand at 5985.671 kb on - strand at 5985.671 kb on - strand at 5985.671 kb on - strand at 5985.684 kb on + strand at 5985.733 kb on + strand at 5985.733 kb on + strand at 5985.733 kb on + strand at 5985.734 kb on - strand at 5985.734 kb on - strand at 5985.734 kb on - strand at 5985.789 kb on + strand at 5985.790 kb on - strand at 5985.898 kb on - strand, within PP_5245 at 5985.921 kb on + strand, within PP_5245 at 5985.921 kb on + strand, within PP_5245 at 5985.995 kb on + strand, within PP_5245 at 5985.996 kb on - strand, within PP_5245
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1 remove 5,983,536 - PP_5242 0.83 -0.5 5,983,623 + PP_5242 0.85 +0.6 5,983,624 - PP_5242 0.85 -0.4 5,983,641 - PP_5242 0.85 +0.1 5,984,110 + +0.0 5,984,164 + -0.1 5,984,248 - -0.2 5,984,263 + -0.7 5,984,263 + +0.1 5,984,264 - -0.0 5,984,305 + +0.3 5,984,305 + -0.9 5,984,306 - -0.1 5,984,403 - +0.4 5,984,403 - +0.2 5,984,439 + -0.4 5,984,451 + -0.4 5,984,481 + +0.0 5,984,481 + -0.8 5,984,482 - -0.2 5,984,482 - -0.2 5,984,489 + -0.0 5,984,489 + -0.0 5,984,490 - -0.7 5,984,515 + +0.5 5,984,516 - +0.8 5,984,658 + PP_5243 0.26 -0.3 5,984,658 + PP_5243 0.26 -0.2 5,984,875 + PP_5243 0.61 +0.0 5,984,875 + PP_5243 0.61 -0.7 5,984,876 - PP_5243 0.61 -1.1 5,985,103 + +0.1 5,985,103 + +0.0 5,985,103 + +0.3 5,985,171 + +0.4 5,985,171 + +0.4 5,985,182 - +0.5 5,985,193 - +0.6 5,985,308 - PP_5244 0.29 -0.0 5,985,308 - PP_5244 0.29 +1.0 5,985,463 + PP_5244 0.67 -0.0 5,985,463 + PP_5244 0.67 +0.1 5,985,464 - PP_5244 0.67 +0.4 5,985,549 + PP_5244 0.88 +0.0 5,985,549 + PP_5244 0.88 +0.3 5,985,549 + PP_5244 0.88 +0.3 5,985,550 - PP_5244 0.89 +0.1 5,985,630 + +0.5 5,985,631 - -0.5 5,985,670 + +0.1 5,985,671 - -0.1 5,985,671 - -0.3 5,985,671 - +0.2 5,985,671 - -0.5 5,985,684 + -0.1 5,985,733 + +0.4 5,985,733 + +0.4 5,985,733 + -0.1 5,985,734 - +0.3 5,985,734 - +1.0 5,985,734 - +0.1 5,985,789 + -0.5 5,985,790 - -0.5 5,985,898 - PP_5245 0.17 -0.2 5,985,921 + PP_5245 0.19 -0.2 5,985,921 + PP_5245 0.19 +0.2 5,985,995 + PP_5245 0.26 -0.3 5,985,996 - PP_5245 0.27 +0.2
Or see this region's nucleotide sequence