Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5130 and PP_5131 are separated by 244 nucleotides PP_5131 and PP_5132 are separated by 131 nucleotides PP_5132 and PP_5133 are separated by 54 nucleotides
PP_5130: PP_5130 - phosphoethanolamine/phosphocholine phosphatase, at 5,852,681 to 5,853,736
_5130
PP_5131: PP_5131 - sodium-cystine symporter, at 5,853,981 to 5,855,372
_5131
PP_5132: PP_5132 - dihydrofolate reductase, at 5,855,504 to 5,856,019
_5132
PP_5133: PP_5133 - conserved membrane protein of unknown function, at 5,856,074 to 5,857,465
_5133
Position (kb)
5853
5854
5855
5856 Strain fitness (log2 ratio)
-2
-1
0
1 at 5853.004 kb on + strand, within PP_5130 at 5853.018 kb on + strand, within PP_5130 at 5853.018 kb on + strand, within PP_5130 at 5853.019 kb on - strand, within PP_5130 at 5853.120 kb on + strand, within PP_5130 at 5853.120 kb on + strand, within PP_5130 at 5853.120 kb on + strand, within PP_5130 at 5853.120 kb on + strand, within PP_5130 at 5853.121 kb on - strand, within PP_5130 at 5853.121 kb on - strand, within PP_5130 at 5853.121 kb on - strand, within PP_5130 at 5853.126 kb on + strand, within PP_5130 at 5853.126 kb on + strand, within PP_5130 at 5853.127 kb on - strand, within PP_5130 at 5853.127 kb on - strand, within PP_5130 at 5853.127 kb on - strand, within PP_5130 at 5853.127 kb on - strand, within PP_5130 at 5853.127 kb on - strand, within PP_5130 at 5853.127 kb on - strand, within PP_5130 at 5853.127 kb on - strand, within PP_5130 at 5853.183 kb on + strand, within PP_5130 at 5853.183 kb on + strand, within PP_5130 at 5853.183 kb on + strand, within PP_5130 at 5853.183 kb on + strand, within PP_5130 at 5853.183 kb on + strand, within PP_5130 at 5853.216 kb on + strand, within PP_5130 at 5853.216 kb on + strand, within PP_5130 at 5853.216 kb on + strand, within PP_5130 at 5853.217 kb on - strand, within PP_5130 at 5853.217 kb on - strand, within PP_5130 at 5853.217 kb on - strand, within PP_5130 at 5853.217 kb on - strand, within PP_5130 at 5853.217 kb on - strand, within PP_5130 at 5853.286 kb on - strand, within PP_5130 at 5853.288 kb on + strand, within PP_5130 at 5853.288 kb on + strand, within PP_5130 at 5853.288 kb on + strand, within PP_5130 at 5853.288 kb on + strand, within PP_5130 at 5853.289 kb on - strand, within PP_5130 at 5853.289 kb on - strand, within PP_5130 at 5853.356 kb on - strand, within PP_5130 at 5853.356 kb on - strand, within PP_5130 at 5853.388 kb on - strand, within PP_5130 at 5853.420 kb on + strand, within PP_5130 at 5853.421 kb on - strand, within PP_5130 at 5853.421 kb on - strand, within PP_5130 at 5853.421 kb on - strand, within PP_5130 at 5853.423 kb on + strand, within PP_5130 at 5853.423 kb on + strand, within PP_5130 at 5853.424 kb on - strand, within PP_5130 at 5853.424 kb on - strand, within PP_5130 at 5853.424 kb on - strand, within PP_5130 at 5853.424 kb on - strand, within PP_5130 at 5853.424 kb on - strand, within PP_5130 at 5853.426 kb on + strand, within PP_5130 at 5853.426 kb on + strand, within PP_5130 at 5853.506 kb on + strand, within PP_5130 at 5853.555 kb on + strand, within PP_5130 at 5853.561 kb on + strand, within PP_5130 at 5853.591 kb on + strand, within PP_5130 at 5853.591 kb on + strand, within PP_5130 at 5853.591 kb on + strand, within PP_5130 at 5853.595 kb on + strand, within PP_5130 at 5853.596 kb on - strand, within PP_5130 at 5853.596 kb on - strand, within PP_5130 at 5853.596 kb on - strand, within PP_5130 at 5853.739 kb on - strand at 5853.739 kb on - strand at 5853.739 kb on - strand at 5853.768 kb on + strand at 5853.769 kb on - strand at 5853.916 kb on - strand at 5854.078 kb on + strand at 5854.078 kb on + strand at 5854.079 kb on - strand at 5854.079 kb on - strand at 5854.079 kb on - strand at 5854.124 kb on + strand, within PP_5131 at 5854.124 kb on + strand, within PP_5131 at 5854.124 kb on + strand, within PP_5131 at 5854.125 kb on - strand, within PP_5131 at 5854.125 kb on - strand, within PP_5131 at 5854.131 kb on + strand, within PP_5131 at 5854.132 kb on - strand, within PP_5131 at 5854.195 kb on - strand, within PP_5131 at 5854.195 kb on - strand, within PP_5131 at 5854.208 kb on - strand, within PP_5131 at 5854.723 kb on + strand, within PP_5131 at 5854.723 kb on + strand, within PP_5131 at 5854.724 kb on - strand, within PP_5131 at 5855.065 kb on + strand, within PP_5131 at 5855.066 kb on - strand, within PP_5131 at 5855.457 kb on - strand at 5856.014 kb on - strand at 5856.014 kb on - strand at 5856.014 kb on - strand at 5856.014 kb on - strand at 5856.032 kb on - strand at 5856.199 kb on - strand at 5856.199 kb on - strand at 5856.199 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1 remove 5,853,004 + PP_5130 0.31 -0.3 5,853,018 + PP_5130 0.32 -0.2 5,853,018 + PP_5130 0.32 +0.4 5,853,019 - PP_5130 0.32 +0.2 5,853,120 + PP_5130 0.42 +0.3 5,853,120 + PP_5130 0.42 -0.5 5,853,120 + PP_5130 0.42 -0.3 5,853,120 + PP_5130 0.42 -0.0 5,853,121 - PP_5130 0.42 -0.1 5,853,121 - PP_5130 0.42 -0.0 5,853,121 - PP_5130 0.42 -0.2 5,853,126 + PP_5130 0.42 +0.8 5,853,126 + PP_5130 0.42 -0.1 5,853,127 - PP_5130 0.42 -0.4 5,853,127 - PP_5130 0.42 -0.8 5,853,127 - PP_5130 0.42 -0.1 5,853,127 - PP_5130 0.42 +0.1 5,853,127 - PP_5130 0.42 -0.1 5,853,127 - PP_5130 0.42 -0.1 5,853,127 - PP_5130 0.42 -0.0 5,853,183 + PP_5130 0.48 -0.7 5,853,183 + PP_5130 0.48 -0.3 5,853,183 + PP_5130 0.48 +0.3 5,853,183 + PP_5130 0.48 -0.0 5,853,183 + PP_5130 0.48 -1.0 5,853,216 + PP_5130 0.51 -0.2 5,853,216 + PP_5130 0.51 +0.0 5,853,216 + PP_5130 0.51 -0.8 5,853,217 - PP_5130 0.51 +0.1 5,853,217 - PP_5130 0.51 -0.2 5,853,217 - PP_5130 0.51 +0.4 5,853,217 - PP_5130 0.51 +0.2 5,853,217 - PP_5130 0.51 -0.4 5,853,286 - PP_5130 0.57 -0.4 5,853,288 + PP_5130 0.57 -0.1 5,853,288 + PP_5130 0.57 -0.3 5,853,288 + PP_5130 0.57 -0.0 5,853,288 + PP_5130 0.57 -0.1 5,853,289 - PP_5130 0.58 -0.1 5,853,289 - PP_5130 0.58 +0.1 5,853,356 - PP_5130 0.64 +0.2 5,853,356 - PP_5130 0.64 +0.1 5,853,388 - PP_5130 0.67 +0.6 5,853,420 + PP_5130 0.70 -2.0 5,853,421 - PP_5130 0.70 -0.2 5,853,421 - PP_5130 0.70 +0.1 5,853,421 - PP_5130 0.70 +0.2 5,853,423 + PP_5130 0.70 -0.1 5,853,423 + PP_5130 0.70 -0.3 5,853,424 - PP_5130 0.70 -0.1 5,853,424 - PP_5130 0.70 +0.4 5,853,424 - PP_5130 0.70 -0.2 5,853,424 - PP_5130 0.70 -0.7 5,853,424 - PP_5130 0.70 -1.0 5,853,426 + PP_5130 0.71 +0.2 5,853,426 + PP_5130 0.71 -0.0 5,853,506 + PP_5130 0.78 +0.6 5,853,555 + PP_5130 0.83 -0.0 5,853,561 + PP_5130 0.83 -0.3 5,853,591 + PP_5130 0.86 -0.2 5,853,591 + PP_5130 0.86 -2.1 5,853,591 + PP_5130 0.86 +0.2 5,853,595 + PP_5130 0.87 +0.1 5,853,596 - PP_5130 0.87 -0.2 5,853,596 - PP_5130 0.87 +0.5 5,853,596 - PP_5130 0.87 +0.4 5,853,739 - -0.3 5,853,739 - -0.3 5,853,739 - -0.6 5,853,768 + -0.2 5,853,769 - -0.2 5,853,916 - -0.6 5,854,078 + -0.1 5,854,078 + -0.3 5,854,079 - +0.4 5,854,079 - -0.2 5,854,079 - +0.2 5,854,124 + PP_5131 0.10 +0.2 5,854,124 + PP_5131 0.10 +0.1 5,854,124 + PP_5131 0.10 -0.6 5,854,125 - PP_5131 0.10 +0.6 5,854,125 - PP_5131 0.10 -0.1 5,854,131 + PP_5131 0.11 +0.2 5,854,132 - PP_5131 0.11 -0.6 5,854,195 - PP_5131 0.15 +0.1 5,854,195 - PP_5131 0.15 +0.2 5,854,208 - PP_5131 0.16 +0.4 5,854,723 + PP_5131 0.53 +0.6 5,854,723 + PP_5131 0.53 +0.1 5,854,724 - PP_5131 0.53 +0.3 5,855,065 + PP_5131 0.78 +0.1 5,855,066 - PP_5131 0.78 +0.8 5,855,457 - -0.5 5,856,014 - -1.0 5,856,014 - -0.6 5,856,014 - -0.1 5,856,014 - +0.2 5,856,032 - -0.4 5,856,199 - -0.5 5,856,199 - +0.3 5,856,199 - -0.2
Or see this region's nucleotide sequence