Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3761 and PP_3762 are separated by 247 nucleotides PP_3762 and PP_3763 are separated by 69 nucleotides PP_3763 and PP_3764 are separated by 67 nucleotides
PP_3761: PP_3761 - Sensor histidine kinase/response regulator, at 4,289,119 to 4,292,586
_3761
PP_3762: PP_3762 - Response regulator, at 4,292,834 to 4,293,184
_3762
PP_3763: PP_3763 - putative CobF protein, at 4,293,254 to 4,294,003
_3763
PP_3764: PP_3764 - outer-membrane porin D, at 4,294,071 to 4,295,372
_3764
Position (kb)
4292
4293
4294 Strain fitness (log2 ratio)
-2
-1
0
1 at 4291.937 kb on + strand, within PP_3761 at 4291.937 kb on + strand, within PP_3761 at 4291.938 kb on - strand, within PP_3761 at 4291.938 kb on - strand, within PP_3761 at 4291.938 kb on - strand, within PP_3761 at 4291.938 kb on - strand, within PP_3761 at 4291.938 kb on - strand, within PP_3761 at 4291.938 kb on - strand, within PP_3761 at 4291.976 kb on + strand, within PP_3761 at 4291.977 kb on - strand, within PP_3761 at 4292.162 kb on + strand, within PP_3761 at 4292.468 kb on + strand at 4292.468 kb on + strand at 4292.469 kb on - strand at 4292.532 kb on + strand at 4292.532 kb on + strand at 4292.604 kb on - strand at 4292.646 kb on + strand at 4292.668 kb on + strand at 4292.669 kb on - strand at 4292.679 kb on - strand at 4292.749 kb on + strand at 4292.749 kb on + strand at 4292.749 kb on + strand at 4292.750 kb on - strand at 4292.750 kb on - strand at 4292.750 kb on - strand at 4292.794 kb on + strand at 4292.803 kb on + strand at 4292.803 kb on + strand at 4292.804 kb on - strand at 4292.804 kb on - strand at 4292.868 kb on + strand at 4292.868 kb on + strand at 4292.869 kb on - strand, within PP_3762 at 4292.869 kb on - strand, within PP_3762 at 4292.906 kb on + strand, within PP_3762 at 4292.906 kb on + strand, within PP_3762 at 4292.949 kb on + strand, within PP_3762 at 4293.166 kb on + strand at 4293.166 kb on + strand at 4293.167 kb on - strand at 4293.210 kb on - strand at 4293.276 kb on + strand at 4293.276 kb on + strand at 4293.276 kb on + strand at 4293.276 kb on + strand at 4293.277 kb on - strand at 4293.277 kb on - strand at 4293.277 kb on - strand at 4293.277 kb on - strand at 4293.277 kb on - strand at 4293.277 kb on - strand at 4293.277 kb on - strand at 4293.280 kb on + strand at 4293.280 kb on + strand at 4293.281 kb on - strand at 4293.281 kb on - strand at 4293.299 kb on - strand at 4293.391 kb on - strand, within PP_3763 at 4293.402 kb on + strand, within PP_3763 at 4293.403 kb on - strand, within PP_3763 at 4293.403 kb on - strand, within PP_3763 at 4293.403 kb on - strand, within PP_3763 at 4293.403 kb on - strand, within PP_3763 at 4293.636 kb on - strand, within PP_3763 at 4293.636 kb on - strand, within PP_3763 at 4293.729 kb on + strand, within PP_3763 at 4293.774 kb on + strand, within PP_3763 at 4293.774 kb on + strand, within PP_3763 at 4293.825 kb on + strand, within PP_3763 at 4293.948 kb on + strand at 4293.948 kb on + strand at 4293.948 kb on + strand at 4293.948 kb on + strand at 4293.949 kb on - strand at 4293.949 kb on - strand at 4293.949 kb on - strand at 4294.052 kb on + strand at 4294.053 kb on - strand at 4294.053 kb on - strand at 4294.072 kb on + strand at 4294.073 kb on - strand at 4294.073 kb on - strand at 4294.073 kb on - strand at 4294.073 kb on - strand at 4294.073 kb on - strand at 4294.090 kb on + strand at 4294.090 kb on + strand at 4294.090 kb on + strand at 4294.091 kb on - strand at 4294.091 kb on - strand at 4294.138 kb on + strand at 4294.138 kb on + strand at 4294.138 kb on + strand at 4294.138 kb on + strand at 4294.139 kb on - strand at 4294.139 kb on - strand at 4294.139 kb on - strand at 4294.151 kb on - strand at 4294.161 kb on - strand at 4294.161 kb on - strand at 4294.170 kb on + strand at 4294.171 kb on - strand at 4294.171 kb on - strand at 4294.171 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1 remove 4,291,937 + PP_3761 0.81 +0.9 4,291,937 + PP_3761 0.81 -0.3 4,291,938 - PP_3761 0.81 -0.4 4,291,938 - PP_3761 0.81 +0.8 4,291,938 - PP_3761 0.81 -1.0 4,291,938 - PP_3761 0.81 -0.7 4,291,938 - PP_3761 0.81 -0.4 4,291,938 - PP_3761 0.81 +0.1 4,291,976 + PP_3761 0.82 +0.0 4,291,977 - PP_3761 0.82 -0.8 4,292,162 + PP_3761 0.88 +0.1 4,292,468 + -0.5 4,292,468 + -0.2 4,292,469 - +0.5 4,292,532 + +0.1 4,292,532 + +0.3 4,292,604 - -0.1 4,292,646 + -0.0 4,292,668 + -0.0 4,292,669 - +0.7 4,292,679 - -0.1 4,292,749 + +0.2 4,292,749 + -0.8 4,292,749 + -0.1 4,292,750 - +0.3 4,292,750 - +0.5 4,292,750 - -0.1 4,292,794 + +0.3 4,292,803 + -0.4 4,292,803 + +0.0 4,292,804 - -0.1 4,292,804 - -0.7 4,292,868 + +0.3 4,292,868 + +0.1 4,292,869 - PP_3762 0.10 -1.1 4,292,869 - PP_3762 0.10 -0.2 4,292,906 + PP_3762 0.21 -0.4 4,292,906 + PP_3762 0.21 -0.4 4,292,949 + PP_3762 0.33 -0.6 4,293,166 + -0.1 4,293,166 + -1.0 4,293,167 - +0.2 4,293,210 - +0.3 4,293,276 + -0.1 4,293,276 + +0.2 4,293,276 + +0.1 4,293,276 + -0.4 4,293,277 - -0.4 4,293,277 - -0.1 4,293,277 - +0.1 4,293,277 - -0.9 4,293,277 - -0.6 4,293,277 - -0.2 4,293,277 - +0.5 4,293,280 + -0.8 4,293,280 + +0.0 4,293,281 - -0.4 4,293,281 - +0.3 4,293,299 - -1.2 4,293,391 - PP_3763 0.18 +0.3 4,293,402 + PP_3763 0.20 -0.2 4,293,403 - PP_3763 0.20 -0.2 4,293,403 - PP_3763 0.20 -0.5 4,293,403 - PP_3763 0.20 -0.1 4,293,403 - PP_3763 0.20 +0.6 4,293,636 - PP_3763 0.51 -0.3 4,293,636 - PP_3763 0.51 -0.1 4,293,729 + PP_3763 0.63 -0.4 4,293,774 + PP_3763 0.69 +1.0 4,293,774 + PP_3763 0.69 -0.0 4,293,825 + PP_3763 0.76 +0.3 4,293,948 + -0.3 4,293,948 + -0.1 4,293,948 + -0.6 4,293,948 + +0.0 4,293,949 - +0.3 4,293,949 - -0.6 4,293,949 - -0.8 4,294,052 + +0.1 4,294,053 - +0.3 4,294,053 - -0.1 4,294,072 + -0.3 4,294,073 - -0.7 4,294,073 - +0.2 4,294,073 - -0.4 4,294,073 - +0.4 4,294,073 - -0.3 4,294,090 + -0.3 4,294,090 + +0.0 4,294,090 + -0.4 4,294,091 - -0.0 4,294,091 - -0.0 4,294,138 + +0.2 4,294,138 + -0.2 4,294,138 + -0.3 4,294,138 + +0.6 4,294,139 - -1.0 4,294,139 - +0.1 4,294,139 - -0.3 4,294,151 - -0.3 4,294,161 - -0.1 4,294,161 - -2.1 4,294,170 + -0.2 4,294,171 - +0.6 4,294,171 - -0.5 4,294,171 - -0.1
Or see this region's nucleotide sequence