Strain Fitness in Pseudomonas putida KT2440 around PP_1609

Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1608 and PP_1609 are separated by 45 nucleotidesPP_1609 and PP_1610 are separated by 81 nucleotides PP_1608: PP_1608 - tRNA(Ile)-lysidine synthase, at 1,804,767 to 1,806,050 _1608 PP_1609: PP_1609 - conserved protein of unknown function, at 1,806,096 to 1,806,236 _1609 PP_1610: PP_1610 - CTP synthase, at 1,806,318 to 1,807,946 _1610 Position (kb) 1806 1807Strain fitness (log2 ratio) -1 0 1at 1806.086 kb on + strandat 1806.128 kb on + strand, within PP_1609at 1806.188 kb on + strand, within PP_1609at 1806.199 kb on + strand, within PP_1609

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Per-strain Table

Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1
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1,806,086 + -0.2
1,806,128 + PP_1609 0.23 +0.4
1,806,188 + PP_1609 0.65 +0.4
1,806,199 + PP_1609 0.73 +0.2

Or see this region's nucleotide sequence