Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhhF and yhhL overlap by 11 nucleotides yhhL and yhhM are separated by 2 nucleotides yhhM and yhhN are separated by 140 nucleotides yhhN and zntA are separated by 73 nucleotides
b3465: yhhF - predicted methyltransferase (NCBI), at 3,602,416 to 3,603,012
yhhF
b3466: yhhL - conserved inner membrane protein (NCBI), at 3,603,002 to 3,603,271
yhhL
b3467: yhhM - hypothetical protein (NCBI), at 3,603,274 to 3,603,633
yhhM
b3468: yhhN - conserved inner membrane protein (NCBI), at 3,603,774 to 3,604,400
yhhN
b3469: zntA - zinc, cobalt and lead efflux system (NCBI), at 3,604,474 to 3,606,672
zntA
Position (kb)
3603
3604
3605 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3602.780 kb on - strand, within yhhF at 3602.780 kb on - strand, within yhhF at 3602.783 kb on - strand, within yhhF at 3602.882 kb on - strand, within yhhF at 3602.907 kb on + strand, within yhhF at 3602.947 kb on - strand, within yhhF at 3602.992 kb on - strand at 3602.993 kb on + strand at 3603.015 kb on - strand at 3603.062 kb on - strand, within yhhL at 3603.062 kb on - strand, within yhhL at 3603.065 kb on + strand, within yhhL at 3603.065 kb on + strand, within yhhL at 3603.083 kb on - strand, within yhhL at 3603.134 kb on + strand, within yhhL at 3603.134 kb on + strand, within yhhL at 3603.135 kb on + strand, within yhhL at 3603.135 kb on + strand, within yhhL at 3603.143 kb on - strand, within yhhL at 3603.159 kb on - strand, within yhhL at 3603.225 kb on + strand, within yhhL at 3603.247 kb on - strand at 3603.279 kb on - strand at 3603.279 kb on - strand at 3603.314 kb on - strand, within yhhM at 3603.314 kb on - strand, within yhhM at 3603.316 kb on + strand, within yhhM at 3603.316 kb on + strand, within yhhM at 3603.339 kb on + strand, within yhhM at 3603.352 kb on + strand, within yhhM at 3603.357 kb on + strand, within yhhM at 3603.360 kb on - strand, within yhhM at 3603.410 kb on - strand, within yhhM at 3603.420 kb on + strand, within yhhM at 3603.423 kb on + strand, within yhhM at 3603.435 kb on - strand, within yhhM at 3603.470 kb on + strand, within yhhM at 3603.678 kb on + strand at 3603.726 kb on + strand at 3603.776 kb on + strand at 3603.812 kb on + strand at 3603.825 kb on + strand at 3603.825 kb on + strand at 3603.945 kb on + strand, within yhhN at 3603.945 kb on + strand, within yhhN at 3603.955 kb on - strand, within yhhN at 3603.960 kb on + strand, within yhhN at 3603.960 kb on + strand, within yhhN at 3603.968 kb on - strand, within yhhN at 3603.980 kb on - strand, within yhhN at 3603.980 kb on - strand, within yhhN at 3603.996 kb on + strand, within yhhN at 3603.996 kb on + strand, within yhhN at 3604.037 kb on + strand, within yhhN at 3604.037 kb on + strand, within yhhN at 3604.037 kb on + strand, within yhhN at 3604.037 kb on + strand, within yhhN at 3604.037 kb on + strand, within yhhN at 3604.045 kb on - strand, within yhhN at 3604.045 kb on - strand, within yhhN at 3604.045 kb on - strand, within yhhN at 3604.071 kb on + strand, within yhhN at 3604.071 kb on + strand, within yhhN at 3604.087 kb on + strand, within yhhN at 3604.087 kb on + strand, within yhhN at 3604.087 kb on + strand, within yhhN at 3604.087 kb on + strand, within yhhN at 3604.092 kb on - strand, within yhhN at 3604.092 kb on - strand, within yhhN at 3604.095 kb on - strand, within yhhN at 3604.095 kb on - strand, within yhhN at 3604.095 kb on - strand, within yhhN at 3604.095 kb on - strand, within yhhN at 3604.095 kb on - strand, within yhhN at 3604.095 kb on - strand, within yhhN at 3604.095 kb on - strand, within yhhN at 3604.107 kb on + strand, within yhhN at 3604.107 kb on + strand, within yhhN at 3604.119 kb on + strand, within yhhN at 3604.157 kb on - strand, within yhhN at 3604.167 kb on + strand, within yhhN at 3604.167 kb on + strand, within yhhN at 3604.170 kb on - strand, within yhhN at 3604.170 kb on - strand, within yhhN at 3604.193 kb on + strand, within yhhN at 3604.193 kb on + strand, within yhhN at 3604.193 kb on + strand, within yhhN at 3604.199 kb on - strand, within yhhN at 3604.229 kb on - strand, within yhhN at 3604.229 kb on - strand, within yhhN at 3604.360 kb on - strand at 3604.387 kb on + strand at 3604.392 kb on - strand at 3604.392 kb on - strand at 3604.423 kb on - strand at 3604.549 kb on + strand at 3604.586 kb on + strand at 3604.594 kb on - strand at 3604.594 kb on - strand at 3604.594 kb on - strand at 3604.639 kb on + strand at 3604.716 kb on + strand, within zntA at 3604.716 kb on + strand, within zntA at 3604.780 kb on + strand, within zntA at 3604.808 kb on + strand, within zntA at 3604.808 kb on + strand, within zntA at 3604.973 kb on - strand, within zntA at 3604.973 kb on - strand, within zntA at 3604.999 kb on + strand, within zntA at 3605.025 kb on - strand, within zntA at 3605.025 kb on - strand, within zntA at 3605.049 kb on - strand, within zntA at 3605.049 kb on - strand, within zntA at 3605.058 kb on + strand, within zntA at 3605.202 kb on - strand, within zntA at 3605.207 kb on - strand, within zntA at 3605.207 kb on - strand, within zntA at 3605.298 kb on + strand, within zntA at 3605.307 kb on - strand, within zntA at 3605.368 kb on + strand, within zntA
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 3,602,780 - yhhF b3465 0.61 +0.1 3,602,780 - yhhF b3465 0.61 +0.4 3,602,783 - yhhF b3465 0.61 -0.0 3,602,882 - yhhF b3465 0.78 -1.0 3,602,907 + yhhF b3465 0.82 +0.4 3,602,947 - yhhF b3465 0.89 +0.9 3,602,992 - +1.1 3,602,993 + +0.3 3,603,015 - -2.2 3,603,062 - yhhL b3466 0.22 +0.3 3,603,062 - yhhL b3466 0.22 -0.2 3,603,065 + yhhL b3466 0.23 +0.4 3,603,065 + yhhL b3466 0.23 +0.2 3,603,083 - yhhL b3466 0.30 +0.2 3,603,134 + yhhL b3466 0.49 -0.2 3,603,134 + yhhL b3466 0.49 +0.4 3,603,135 + yhhL b3466 0.49 +0.1 3,603,135 + yhhL b3466 0.49 +0.4 3,603,143 - yhhL b3466 0.52 +0.5 3,603,159 - yhhL b3466 0.58 +0.1 3,603,225 + yhhL b3466 0.83 +0.3 3,603,247 - -0.6 3,603,279 - -0.4 3,603,279 - +0.2 3,603,314 - yhhM b3467 0.11 -0.1 3,603,314 - yhhM b3467 0.11 -0.5 3,603,316 + yhhM b3467 0.12 +0.2 3,603,316 + yhhM b3467 0.12 -0.1 3,603,339 + yhhM b3467 0.18 -1.4 3,603,352 + yhhM b3467 0.22 +0.1 3,603,357 + yhhM b3467 0.23 +0.6 3,603,360 - yhhM b3467 0.24 -0.2 3,603,410 - yhhM b3467 0.38 +0.2 3,603,420 + yhhM b3467 0.41 -0.2 3,603,423 + yhhM b3467 0.41 +0.4 3,603,435 - yhhM b3467 0.45 -1.7 3,603,470 + yhhM b3467 0.54 -0.8 3,603,678 + -0.6 3,603,726 + -0.2 3,603,776 + -0.2 3,603,812 + -0.3 3,603,825 + -0.5 3,603,825 + +0.1 3,603,945 + yhhN b3468 0.27 +1.1 3,603,945 + yhhN b3468 0.27 -3.9 3,603,955 - yhhN b3468 0.29 +0.1 3,603,960 + yhhN b3468 0.30 +1.4 3,603,960 + yhhN b3468 0.30 -2.3 3,603,968 - yhhN b3468 0.31 -0.4 3,603,980 - yhhN b3468 0.33 -0.7 3,603,980 - yhhN b3468 0.33 +0.0 3,603,996 + yhhN b3468 0.35 -0.1 3,603,996 + yhhN b3468 0.35 -0.6 3,604,037 + yhhN b3468 0.42 -0.4 3,604,037 + yhhN b3468 0.42 -0.4 3,604,037 + yhhN b3468 0.42 -0.6 3,604,037 + yhhN b3468 0.42 +0.4 3,604,037 + yhhN b3468 0.42 -0.0 3,604,045 - yhhN b3468 0.43 -0.4 3,604,045 - yhhN b3468 0.43 -0.3 3,604,045 - yhhN b3468 0.43 +0.3 3,604,071 + yhhN b3468 0.47 -0.3 3,604,071 + yhhN b3468 0.47 -0.1 3,604,087 + yhhN b3468 0.50 -0.1 3,604,087 + yhhN b3468 0.50 +0.4 3,604,087 + yhhN b3468 0.50 -0.3 3,604,087 + yhhN b3468 0.50 +0.2 3,604,092 - yhhN b3468 0.51 -1.7 3,604,092 - yhhN b3468 0.51 +0.3 3,604,095 - yhhN b3468 0.51 -0.1 3,604,095 - yhhN b3468 0.51 -0.8 3,604,095 - yhhN b3468 0.51 +0.3 3,604,095 - yhhN b3468 0.51 +0.4 3,604,095 - yhhN b3468 0.51 -0.0 3,604,095 - yhhN b3468 0.51 +0.8 3,604,095 - yhhN b3468 0.51 -0.3 3,604,107 + yhhN b3468 0.53 +0.5 3,604,107 + yhhN b3468 0.53 -0.5 3,604,119 + yhhN b3468 0.55 -0.4 3,604,157 - yhhN b3468 0.61 -0.1 3,604,167 + yhhN b3468 0.63 +0.4 3,604,167 + yhhN b3468 0.63 -0.0 3,604,170 - yhhN b3468 0.63 -1.5 3,604,170 - yhhN b3468 0.63 -1.1 3,604,193 + yhhN b3468 0.67 -0.2 3,604,193 + yhhN b3468 0.67 -0.0 3,604,193 + yhhN b3468 0.67 -0.6 3,604,199 - yhhN b3468 0.68 -0.3 3,604,229 - yhhN b3468 0.73 +0.4 3,604,229 - yhhN b3468 0.73 -0.7 3,604,360 - -0.8 3,604,387 + -0.3 3,604,392 - -1.0 3,604,392 - +0.6 3,604,423 - -1.8 3,604,549 + -1.5 3,604,586 + -1.5 3,604,594 - -2.6 3,604,594 - -0.1 3,604,594 - -2.7 3,604,639 + -0.9 3,604,716 + zntA b3469 0.11 -0.7 3,604,716 + zntA b3469 0.11 -1.7 3,604,780 + zntA b3469 0.14 -1.1 3,604,808 + zntA b3469 0.15 -1.7 3,604,808 + zntA b3469 0.15 -2.7 3,604,973 - zntA b3469 0.23 +0.0 3,604,973 - zntA b3469 0.23 -1.5 3,604,999 + zntA b3469 0.24 -1.1 3,605,025 - zntA b3469 0.25 -1.8 3,605,025 - zntA b3469 0.25 -1.0 3,605,049 - zntA b3469 0.26 -2.4 3,605,049 - zntA b3469 0.26 -0.4 3,605,058 + zntA b3469 0.27 -1.0 3,605,202 - zntA b3469 0.33 +0.0 3,605,207 - zntA b3469 0.33 -2.2 3,605,207 - zntA b3469 0.33 -1.7 3,605,298 + zntA b3469 0.37 -1.1 3,605,307 - zntA b3469 0.38 -1.7 3,605,368 + zntA b3469 0.41 -2.4
Or see this region's nucleotide sequence