Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mhpA and mhpB are separated by 1 nucleotides mhpB and mhpC are separated by 2 nucleotides mhpC and mhpD are separated by 9 nucleotides
b0347: mhpA - 3-(3-hydroxyphenyl)propionate hydroxylase (NCBI), at 367,835 to 369,499
mhpA
b0348: mhpB - 2,3-dihydroxyphenylpropionate 1,2-dioxygenase (NCBI), at 369,501 to 370,445
mhpB
b0349: mhpC - 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase (VIMSS), at 370,448 to 371,329
mhpC
b0350: mhpD - 2-keto-4-pentenoate hydratase (VIMSS), at 371,339 to 372,148
mhpD
Position (kb) 369
370
371 Strain fitness (log2 ratio) -1
0
1 at 368.568 kb on - strand, within mhpA at 368.661 kb on - strand, within mhpA at 368.728 kb on + strand, within mhpA at 368.728 kb on + strand, within mhpA at 368.755 kb on + strand, within mhpA at 368.761 kb on - strand, within mhpA at 368.763 kb on + strand, within mhpA at 368.763 kb on + strand, within mhpA at 368.778 kb on + strand, within mhpA at 368.883 kb on - strand, within mhpA at 368.898 kb on - strand, within mhpA at 368.898 kb on - strand, within mhpA at 369.036 kb on - strand, within mhpA at 369.077 kb on - strand, within mhpA at 369.133 kb on + strand, within mhpA at 369.232 kb on + strand, within mhpA at 369.283 kb on + strand, within mhpA at 369.418 kb on + strand at 369.625 kb on + strand, within mhpB at 369.705 kb on - strand, within mhpB at 369.705 kb on - strand, within mhpB at 369.829 kb on + strand, within mhpB at 369.829 kb on + strand, within mhpB at 369.837 kb on - strand, within mhpB at 369.908 kb on + strand, within mhpB at 369.908 kb on + strand, within mhpB at 370.056 kb on - strand, within mhpB at 370.198 kb on - strand, within mhpB at 370.263 kb on + strand, within mhpB at 370.344 kb on + strand, within mhpB at 370.364 kb on + strand at 370.398 kb on - strand at 370.517 kb on - strand at 370.524 kb on + strand at 370.598 kb on - strand, within mhpC at 370.598 kb on - strand, within mhpC at 370.598 kb on - strand, within mhpC at 370.632 kb on + strand, within mhpC at 370.635 kb on - strand, within mhpC at 370.688 kb on - strand, within mhpC at 370.690 kb on + strand, within mhpC at 370.813 kb on + strand, within mhpC at 370.931 kb on - strand, within mhpC at 371.051 kb on - strand, within mhpC at 371.051 kb on - strand, within mhpC at 371.089 kb on + strand, within mhpC at 371.089 kb on + strand, within mhpC at 371.143 kb on - strand, within mhpC at 371.194 kb on + strand, within mhpC at 371.196 kb on + strand, within mhpC at 371.237 kb on + strand, within mhpC at 371.247 kb on - strand at 371.280 kb on - strand at 371.325 kb on + strand at 371.331 kb on - strand at 371.331 kb on - strand at 371.331 kb on - strand at 371.360 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 368,568 - mhpA b0347 0.44 -0.3 368,661 - mhpA b0347 0.50 +1.5 368,728 + mhpA b0347 0.54 -0.2 368,728 + mhpA b0347 0.54 -0.5 368,755 + mhpA b0347 0.55 -0.5 368,761 - mhpA b0347 0.56 -0.6 368,763 + mhpA b0347 0.56 +0.0 368,763 + mhpA b0347 0.56 +0.8 368,778 + mhpA b0347 0.57 +0.2 368,883 - mhpA b0347 0.63 +0.4 368,898 - mhpA b0347 0.64 -0.1 368,898 - mhpA b0347 0.64 +0.1 369,036 - mhpA b0347 0.72 +0.1 369,077 - mhpA b0347 0.75 -0.4 369,133 + mhpA b0347 0.78 -0.2 369,232 + mhpA b0347 0.84 -0.1 369,283 + mhpA b0347 0.87 -0.1 369,418 + -0.1 369,625 + mhpB b0348 0.13 -0.1 369,705 - mhpB b0348 0.22 +0.2 369,705 - mhpB b0348 0.22 +0.7 369,829 + mhpB b0348 0.35 -0.9 369,829 + mhpB b0348 0.35 +0.5 369,837 - mhpB b0348 0.36 -0.3 369,908 + mhpB b0348 0.43 -0.1 369,908 + mhpB b0348 0.43 -0.3 370,056 - mhpB b0348 0.59 +0.7 370,198 - mhpB b0348 0.74 +0.0 370,263 + mhpB b0348 0.81 +0.2 370,344 + mhpB b0348 0.89 +0.1 370,364 + +0.1 370,398 - -0.1 370,517 - +0.2 370,524 + -0.3 370,598 - mhpC b0349 0.17 +0.0 370,598 - mhpC b0349 0.17 +0.1 370,598 - mhpC b0349 0.17 -0.6 370,632 + mhpC b0349 0.21 +0.1 370,635 - mhpC b0349 0.21 +0.0 370,688 - mhpC b0349 0.27 +0.2 370,690 + mhpC b0349 0.27 -0.2 370,813 + mhpC b0349 0.41 +0.1 370,931 - mhpC b0349 0.55 -0.2 371,051 - mhpC b0349 0.68 -0.6 371,051 - mhpC b0349 0.68 -0.2 371,089 + mhpC b0349 0.73 -0.6 371,089 + mhpC b0349 0.73 +0.1 371,143 - mhpC b0349 0.79 +0.2 371,194 + mhpC b0349 0.85 -0.2 371,196 + mhpC b0349 0.85 +0.3 371,237 + mhpC b0349 0.89 -0.3 371,247 - +0.5 371,280 - +0.2 371,325 + -0.4 371,331 - +0.4 371,331 - -1.3 371,331 - -0.4 371,360 - -0.8
Or see this region's nucleotide sequence