Experiment: Dictyostellum sp.mixed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dacA and rlpA are separated by 138 nucleotides rlpA and mrdB are separated by 10 nucleotides
b0632: dacA - D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI), at 661,975 to 663,186
dacA
b0633: rlpA - minor lipoprotein (NCBI), at 663,325 to 664,413
rlpA
b0634: mrdB - cell wall shape-determining protein (NCBI), at 664,424 to 665,536
mrdB
Position (kb) 663
664
665 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 662.380 kb on - strand, within dacA at 662.380 kb on - strand, within dacA at 662.462 kb on - strand, within dacA at 662.462 kb on - strand, within dacA at 662.609 kb on - strand, within dacA at 662.609 kb on - strand, within dacA at 662.618 kb on + strand, within dacA at 662.618 kb on + strand, within dacA at 662.618 kb on + strand, within dacA at 662.618 kb on + strand, within dacA at 662.618 kb on + strand, within dacA at 662.620 kb on + strand, within dacA at 662.644 kb on + strand, within dacA at 662.644 kb on + strand, within dacA at 662.644 kb on + strand, within dacA at 662.644 kb on + strand, within dacA at 662.652 kb on - strand, within dacA at 662.652 kb on - strand, within dacA at 662.864 kb on + strand, within dacA at 662.867 kb on - strand, within dacA at 662.867 kb on - strand, within dacA at 662.889 kb on - strand, within dacA at 662.889 kb on - strand, within dacA at 663.038 kb on + strand, within dacA at 663.053 kb on - strand, within dacA at 663.060 kb on + strand, within dacA at 663.096 kb on - strand at 663.096 kb on - strand at 663.123 kb on - strand at 663.140 kb on - strand at 663.147 kb on - strand at 663.147 kb on - strand at 663.165 kb on - strand at 663.236 kb on - strand at 663.236 kb on - strand at 663.325 kb on - strand at 663.391 kb on + strand at 663.391 kb on + strand at 663.394 kb on - strand at 663.427 kb on - strand at 663.465 kb on - strand, within rlpA at 663.465 kb on - strand, within rlpA at 663.475 kb on - strand, within rlpA at 663.532 kb on - strand, within rlpA at 663.581 kb on + strand, within rlpA at 663.581 kb on + strand, within rlpA at 663.620 kb on + strand, within rlpA at 663.697 kb on - strand, within rlpA at 663.770 kb on - strand, within rlpA at 663.775 kb on - strand, within rlpA at 663.775 kb on - strand, within rlpA at 663.844 kb on - strand, within rlpA at 663.849 kb on - strand, within rlpA at 663.849 kb on - strand, within rlpA at 663.860 kb on + strand, within rlpA at 663.865 kb on + strand, within rlpA at 663.865 kb on + strand, within rlpA at 663.867 kb on + strand, within rlpA at 663.873 kb on - strand, within rlpA at 663.894 kb on + strand, within rlpA at 663.900 kb on - strand, within rlpA at 663.900 kb on - strand, within rlpA at 663.941 kb on - strand, within rlpA at 664.174 kb on + strand, within rlpA at 664.260 kb on - strand, within rlpA at 664.260 kb on - strand, within rlpA at 664.293 kb on + strand, within rlpA at 664.293 kb on + strand, within rlpA at 664.303 kb on + strand, within rlpA at 664.311 kb on - strand at 664.323 kb on + strand at 664.348 kb on + strand at 664.367 kb on - strand at 664.385 kb on + strand at 664.385 kb on + strand at 664.413 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dictyostellum sp.mixed culture-2 remove 662,380 - dacA b0632 0.33 -1.3 662,380 - dacA b0632 0.33 -3.3 662,462 - dacA b0632 0.40 -1.7 662,462 - dacA b0632 0.40 -4.3 662,609 - dacA b0632 0.52 -2.0 662,609 - dacA b0632 0.52 -3.5 662,618 + dacA b0632 0.53 -2.8 662,618 + dacA b0632 0.53 -2.4 662,618 + dacA b0632 0.53 -3.3 662,618 + dacA b0632 0.53 -2.6 662,618 + dacA b0632 0.53 -1.3 662,620 + dacA b0632 0.53 -2.1 662,644 + dacA b0632 0.55 -2.8 662,644 + dacA b0632 0.55 -1.3 662,644 + dacA b0632 0.55 -2.1 662,644 + dacA b0632 0.55 -1.7 662,652 - dacA b0632 0.56 -2.7 662,652 - dacA b0632 0.56 -1.0 662,864 + dacA b0632 0.73 -1.5 662,867 - dacA b0632 0.74 -2.2 662,867 - dacA b0632 0.74 -2.2 662,889 - dacA b0632 0.75 -1.8 662,889 - dacA b0632 0.75 -2.2 663,038 + dacA b0632 0.88 -3.2 663,053 - dacA b0632 0.89 -1.0 663,060 + dacA b0632 0.90 -2.7 663,096 - -3.5 663,096 - -2.8 663,123 - -1.1 663,140 - -3.0 663,147 - -4.2 663,147 - -1.6 663,165 - -0.9 663,236 - +0.2 663,236 - -0.6 663,325 - -2.9 663,391 + -0.8 663,391 + -1.0 663,394 - -1.3 663,427 - -2.4 663,465 - rlpA b0633 0.13 -0.9 663,465 - rlpA b0633 0.13 -1.4 663,475 - rlpA b0633 0.14 -1.1 663,532 - rlpA b0633 0.19 -0.6 663,581 + rlpA b0633 0.24 -1.2 663,581 + rlpA b0633 0.24 +0.4 663,620 + rlpA b0633 0.27 -0.7 663,697 - rlpA b0633 0.34 -0.6 663,770 - rlpA b0633 0.41 -1.3 663,775 - rlpA b0633 0.41 -1.2 663,775 - rlpA b0633 0.41 -0.4 663,844 - rlpA b0633 0.48 -1.0 663,849 - rlpA b0633 0.48 +0.2 663,849 - rlpA b0633 0.48 -0.7 663,860 + rlpA b0633 0.49 -1.7 663,865 + rlpA b0633 0.50 -1.4 663,865 + rlpA b0633 0.50 +1.4 663,867 + rlpA b0633 0.50 -1.5 663,873 - rlpA b0633 0.50 -0.6 663,894 + rlpA b0633 0.52 -1.3 663,900 - rlpA b0633 0.53 -1.4 663,900 - rlpA b0633 0.53 -1.2 663,941 - rlpA b0633 0.57 -0.6 664,174 + rlpA b0633 0.78 +0.7 664,260 - rlpA b0633 0.86 -1.5 664,260 - rlpA b0633 0.86 -1.2 664,293 + rlpA b0633 0.89 +0.5 664,293 + rlpA b0633 0.89 -1.0 664,303 + rlpA b0633 0.90 -0.6 664,311 - +0.2 664,323 + -1.1 664,348 + -0.2 664,367 - -0.9 664,385 + -1.1 664,385 + -1.6 664,413 + -0.6
Or see this region's nucleotide sequence