Strain Fitness in Escherichia coli BW25113 around b4104

Experiment: Tetrahymena sp., Mixed culture-1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Tetrahymena sp., Mixed culture-1
4,319,573	+	phnG	b4101	0.68	+2.4
4,319,731	-				+0.3
4,319,731	-				-2.5
4,319,803	+	phnF	b4102	0.11	+0.9
4,319,803	+	phnF	b4102	0.11	-1.2
4,319,803	+	phnF	b4102	0.11	-2.7
4,319,811	-	phnF	b4102	0.13	+2.2
4,319,811	-	phnF	b4102	0.13	+0.3
4,319,985	-	phnF	b4102	0.37	+3.0
4,319,999	+	phnF	b4102	0.38	+2.9
4,320,073	-	phnF	b4102	0.49	-0.3
4,320,121	-	phnF	b4102	0.55	-2.1
4,320,253	-	phnF	b4102	0.73	+0.2
4,320,253	-	phnF	b4102	0.73	-2.0
4,320,292	+	phnF	b4102	0.79	+0.0
4,320,326	+	phnF	b4102	0.83	-0.5
4,320,348	-	phnF	b4102	0.87	+1.3
4,320,405	-				-0.2
4,320,412	+				+2.4
4,320,412	+				-3.0
4,320,652	-	phnE	b4104	0.50	+2.1
4,320,670	-	phnE	b4104	0.55	+2.8
4,320,678	-	phnE	b4104	0.57	+1.3
4,320,691	+	phnE	b4104	0.60	-0.5
4,320,804	+				+2.0
4,320,842	+				+0.0
4,320,937	+				+1.6
4,321,008	-				-0.3
4,321,144	-				-0.1
4,321,193	-				-2.9
4,321,193	-				-1.1
4,321,193	-				-0.2
4,321,208	-				+2.2
4,321,248	-				-2.5
4,321,254	+				+1.8
4,321,308	+				+0.5
4,321,310	+				+2.1
4,321,310	+				+1.7
4,321,316	-				+1.5
4,321,421	-				+1.7
4,321,440	-				-1.3
4,321,440	-				-3.8
4,321,512	-	phnD	b4105	0.15	+0.6
4,321,572	+	phnD	b4105	0.21	-4.9
4,321,637	-	phnD	b4105	0.27	+1.5
4,321,637	-	phnD	b4105	0.27	-2.7
4,321,713	-	phnD	b4105	0.35	+2.1
4,321,713	-	phnD	b4105	0.35	+0.7
4,321,716	+	phnD	b4105	0.35	+0.4
4,321,834	+	phnD	b4105	0.47	-2.8
4,321,834	+	phnD	b4105	0.47	-2.6