Experiment: Tetrahymena sp., Mixed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yjdP and phnP are separated by 146 nucleotides phnP and phnO are separated by 1 nucleotides phnO and phnN overlap by 14 nucleotides phnN and phnM overlap by 1 nucleotides
b4487: yjdP - hypothetical protein (NCBI), at 4,311,891 to 4,312,220
yjdP
b4092: phnP - carbon-phosphorus lyase complex accessory protein (NCBI), at 4,312,367 to 4,313,125
phnP
b4093: phnO - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 4,313,127 to 4,313,561
phnO
b4094: phnN - ribose 1,5-bisphosphokinase (NCBI), at 4,313,548 to 4,314,105
phnN
b4095: phnM - carbon-phosphorus lyase complex subunit (NCBI), at 4,314,105 to 4,315,241
phnM
Position (kb)
4313
4314 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 4312.193 kb on + strand at 4312.202 kb on + strand at 4312.227 kb on - strand at 4312.256 kb on + strand at 4312.263 kb on - strand at 4312.338 kb on - strand at 4312.375 kb on + strand at 4312.418 kb on - strand at 4312.418 kb on - strand at 4312.567 kb on + strand, within phnP at 4312.658 kb on - strand, within phnP at 4312.658 kb on - strand, within phnP at 4312.663 kb on - strand, within phnP at 4312.663 kb on - strand, within phnP at 4312.668 kb on - strand, within phnP at 4312.668 kb on - strand, within phnP at 4312.700 kb on + strand, within phnP at 4312.714 kb on - strand, within phnP at 4312.720 kb on + strand, within phnP at 4312.720 kb on + strand, within phnP at 4312.721 kb on - strand, within phnP at 4312.728 kb on - strand, within phnP at 4312.788 kb on - strand, within phnP at 4312.803 kb on + strand, within phnP at 4312.808 kb on + strand, within phnP at 4312.948 kb on - strand, within phnP at 4312.965 kb on - strand, within phnP at 4313.024 kb on - strand, within phnP at 4313.041 kb on + strand, within phnP at 4313.060 kb on + strand at 4313.094 kb on - strand at 4313.115 kb on - strand at 4313.115 kb on - strand at 4313.127 kb on + strand at 4313.185 kb on - strand, within phnO at 4313.195 kb on - strand, within phnO at 4313.274 kb on + strand, within phnO at 4313.433 kb on + strand, within phnO at 4313.502 kb on - strand, within phnO at 4313.502 kb on - strand, within phnO at 4313.529 kb on - strand at 4313.529 kb on - strand at 4313.565 kb on - strand at 4313.634 kb on + strand, within phnN at 4313.662 kb on - strand, within phnN at 4313.821 kb on + strand, within phnN at 4313.821 kb on + strand, within phnN at 4313.881 kb on - strand, within phnN at 4313.881 kb on - strand, within phnN at 4313.956 kb on - strand, within phnN at 4314.001 kb on - strand, within phnN at 4314.043 kb on + strand, within phnN at 4314.122 kb on - strand at 4314.213 kb on + strand at 4314.213 kb on + strand at 4314.357 kb on - strand, within phnM at 4314.363 kb on + strand, within phnM at 4314.396 kb on + strand, within phnM at 4314.445 kb on + strand, within phnM at 4314.445 kb on + strand, within phnM
Per-strain Table
Position Strand Gene LocusTag Fraction Tetrahymena sp., Mixed culture-1 remove 4,312,193 + -2.8 4,312,202 + +1.6 4,312,227 - -3.7 4,312,256 + -4.0 4,312,263 - +0.1 4,312,338 - -2.6 4,312,375 + -2.2 4,312,418 - +0.9 4,312,418 - -3.0 4,312,567 + phnP b4092 0.26 -0.6 4,312,658 - phnP b4092 0.38 -3.4 4,312,658 - phnP b4092 0.38 +0.0 4,312,663 - phnP b4092 0.39 +1.3 4,312,663 - phnP b4092 0.39 -2.7 4,312,668 - phnP b4092 0.40 +1.8 4,312,668 - phnP b4092 0.40 +0.6 4,312,700 + phnP b4092 0.44 -0.2 4,312,714 - phnP b4092 0.46 +0.4 4,312,720 + phnP b4092 0.47 +0.8 4,312,720 + phnP b4092 0.47 -0.8 4,312,721 - phnP b4092 0.47 -3.7 4,312,728 - phnP b4092 0.48 +0.4 4,312,788 - phnP b4092 0.55 +0.4 4,312,803 + phnP b4092 0.57 -2.0 4,312,808 + phnP b4092 0.58 -5.1 4,312,948 - phnP b4092 0.77 +0.1 4,312,965 - phnP b4092 0.79 +0.9 4,313,024 - phnP b4092 0.87 +1.5 4,313,041 + phnP b4092 0.89 +2.3 4,313,060 + +0.6 4,313,094 - -4.0 4,313,115 - +3.5 4,313,115 - -4.1 4,313,127 + -2.6 4,313,185 - phnO b4093 0.13 +0.3 4,313,195 - phnO b4093 0.16 +1.4 4,313,274 + phnO b4093 0.34 +2.2 4,313,433 + phnO b4093 0.70 -0.1 4,313,502 - phnO b4093 0.86 +1.4 4,313,502 - phnO b4093 0.86 +1.7 4,313,529 - +0.3 4,313,529 - -0.1 4,313,565 - +0.6 4,313,634 + phnN b4094 0.15 -2.7 4,313,662 - phnN b4094 0.20 -0.1 4,313,821 + phnN b4094 0.49 -0.7 4,313,821 + phnN b4094 0.49 -2.8 4,313,881 - phnN b4094 0.60 -2.8 4,313,881 - phnN b4094 0.60 -1.0 4,313,956 - phnN b4094 0.73 +1.4 4,314,001 - phnN b4094 0.81 +0.2 4,314,043 + phnN b4094 0.89 +2.0 4,314,122 - -0.0 4,314,213 + -0.3 4,314,213 + -3.1 4,314,357 - phnM b4095 0.22 +0.3 4,314,363 + phnM b4095 0.23 -2.8 4,314,396 + phnM b4095 0.26 +1.6 4,314,445 + phnM b4095 0.30 -3.2 4,314,445 + phnM b4095 0.30 +2.2
Or see this region's nucleotide sequence