Experiment: Tetrahymena sp., Mixed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt atpE and atpB are separated by 46 nucleotides atpB and atpI are separated by 8 nucleotides atpI and gidB are separated by 616 nucleotides
b3737: atpE - F0F1 ATP synthase subunit C (NCBI), at 3,918,973 to 3,919,212
atpE
b3738: atpB - F0F1 ATP synthase subunit A (NCBI), at 3,919,259 to 3,920,074
atpB
b3739: atpI - membrane-bound ATP synthase, dispensable protein, affects expression of atpB (VIMSS), at 3,920,083 to 3,920,463
atpI
b3740: gidB - glucose-inhibited division protein B (NCBI), at 3,921,080 to 3,921,703
gidB
Position (kb)
3920
3921 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 3919.227 kb on - strand at 3919.248 kb on - strand at 3919.265 kb on - strand at 3919.265 kb on - strand at 3920.048 kb on - strand at 3920.063 kb on - strand at 3920.063 kb on - strand at 3920.119 kb on - strand at 3920.212 kb on - strand, within atpI at 3920.212 kb on - strand, within atpI at 3920.223 kb on - strand, within atpI at 3920.270 kb on - strand, within atpI at 3920.270 kb on - strand, within atpI at 3920.362 kb on - strand, within atpI at 3920.362 kb on - strand, within atpI at 3920.362 kb on - strand, within atpI at 3920.365 kb on - strand, within atpI at 3920.375 kb on - strand, within atpI at 3920.408 kb on - strand, within atpI at 3920.410 kb on - strand, within atpI at 3920.412 kb on - strand, within atpI at 3920.425 kb on - strand, within atpI at 3920.462 kb on - strand at 3920.488 kb on - strand at 3920.511 kb on - strand at 3920.514 kb on - strand at 3920.533 kb on - strand at 3920.538 kb on - strand at 3920.558 kb on - strand at 3920.561 kb on - strand at 3920.594 kb on - strand at 3920.594 kb on - strand at 3920.638 kb on - strand at 3920.638 kb on - strand at 3920.638 kb on - strand at 3920.638 kb on - strand at 3920.641 kb on + strand at 3920.649 kb on - strand at 3920.651 kb on + strand at 3920.658 kb on - strand at 3920.675 kb on + strand at 3920.675 kb on + strand at 3920.696 kb on - strand at 3920.696 kb on - strand at 3920.698 kb on + strand at 3920.699 kb on + strand at 3920.699 kb on + strand at 3920.707 kb on + strand at 3920.707 kb on - strand at 3920.717 kb on + strand at 3920.722 kb on + strand at 3920.722 kb on + strand at 3920.722 kb on + strand at 3920.725 kb on - strand at 3920.726 kb on - strand at 3920.726 kb on - strand at 3920.729 kb on - strand at 3920.729 kb on - strand at 3920.751 kb on - strand at 3920.768 kb on + strand at 3920.773 kb on + strand at 3920.867 kb on + strand at 3920.877 kb on + strand at 3920.885 kb on - strand at 3920.885 kb on - strand at 3920.920 kb on - strand at 3920.920 kb on - strand at 3920.963 kb on + strand at 3920.963 kb on + strand at 3920.982 kb on - strand at 3920.985 kb on + strand at 3920.992 kb on + strand at 3920.997 kb on + strand at 3920.997 kb on + strand at 3921.014 kb on - strand at 3921.014 kb on - strand at 3921.014 kb on - strand at 3921.014 kb on - strand at 3921.036 kb on + strand at 3921.039 kb on + strand at 3921.039 kb on + strand at 3921.047 kb on - strand at 3921.101 kb on - strand at 3921.113 kb on - strand at 3921.113 kb on - strand at 3921.122 kb on + strand at 3921.122 kb on + strand at 3921.125 kb on - strand at 3921.125 kb on - strand at 3921.125 kb on - strand at 3921.225 kb on - strand, within gidB at 3921.240 kb on + strand, within gidB at 3921.252 kb on - strand, within gidB at 3921.255 kb on + strand, within gidB at 3921.263 kb on - strand, within gidB at 3921.265 kb on - strand, within gidB at 3921.310 kb on - strand, within gidB at 3921.336 kb on - strand, within gidB at 3921.336 kb on - strand, within gidB at 3921.336 kb on - strand, within gidB at 3921.338 kb on + strand, within gidB at 3921.357 kb on - strand, within gidB at 3921.390 kb on - strand, within gidB at 3921.406 kb on + strand, within gidB at 3921.406 kb on + strand, within gidB at 3921.414 kb on + strand, within gidB at 3921.414 kb on + strand, within gidB at 3921.424 kb on - strand, within gidB at 3921.424 kb on - strand, within gidB at 3921.434 kb on - strand, within gidB at 3921.434 kb on - strand, within gidB at 3921.445 kb on + strand, within gidB at 3921.450 kb on + strand, within gidB
Per-strain Table
Position Strand Gene LocusTag Fraction Tetrahymena sp., Mixed culture-1 remove 3,919,227 - +0.1 3,919,248 - -2.8 3,919,265 - +0.8 3,919,265 - -2.9 3,920,048 - -1.3 3,920,063 - +1.5 3,920,063 - -2.0 3,920,119 - -3.5 3,920,212 - atpI b3739 0.34 +2.6 3,920,212 - atpI b3739 0.34 +1.3 3,920,223 - atpI b3739 0.37 -0.4 3,920,270 - atpI b3739 0.49 -2.2 3,920,270 - atpI b3739 0.49 -1.4 3,920,362 - atpI b3739 0.73 -0.6 3,920,362 - atpI b3739 0.73 +2.4 3,920,362 - atpI b3739 0.73 +2.5 3,920,365 - atpI b3739 0.74 +3.7 3,920,375 - atpI b3739 0.77 +1.6 3,920,408 - atpI b3739 0.85 +1.0 3,920,410 - atpI b3739 0.86 +0.3 3,920,412 - atpI b3739 0.86 +0.3 3,920,425 - atpI b3739 0.90 -1.2 3,920,462 - +0.1 3,920,488 - +1.1 3,920,511 - +0.9 3,920,514 - -1.3 3,920,533 - +2.2 3,920,538 - -2.1 3,920,558 - +0.2 3,920,561 - -0.3 3,920,594 - -2.6 3,920,594 - +3.6 3,920,638 - -3.7 3,920,638 - -1.1 3,920,638 - -3.0 3,920,638 - -0.1 3,920,641 + +1.1 3,920,649 - +1.3 3,920,651 + -2.4 3,920,658 - -0.1 3,920,675 + -0.6 3,920,675 + -3.4 3,920,696 - +1.9 3,920,696 - +1.9 3,920,698 + -0.8 3,920,699 + -4.3 3,920,699 + -3.9 3,920,707 + +0.8 3,920,707 - -1.5 3,920,717 + -2.3 3,920,722 + +1.4 3,920,722 + +1.8 3,920,722 + +0.4 3,920,725 - +1.1 3,920,726 - -2.2 3,920,726 - -2.3 3,920,729 - -1.5 3,920,729 - +5.3 3,920,751 - +2.9 3,920,768 + -1.5 3,920,773 + +1.7 3,920,867 + +0.9 3,920,877 + -4.1 3,920,885 - -1.9 3,920,885 - +1.7 3,920,920 - -5.5 3,920,920 - +0.3 3,920,963 + -1.6 3,920,963 + +0.4 3,920,982 - +0.1 3,920,985 + -1.0 3,920,992 + -2.1 3,920,997 + -2.7 3,920,997 + +0.9 3,921,014 - -2.8 3,921,014 - +1.3 3,921,014 - -3.7 3,921,014 - -2.7 3,921,036 + -0.1 3,921,039 + -2.6 3,921,039 + +5.0 3,921,047 - +0.7 3,921,101 - -3.0 3,921,113 - -2.0 3,921,113 - -1.4 3,921,122 + -0.5 3,921,122 + -4.1 3,921,125 - +0.9 3,921,125 - -3.4 3,921,125 - -3.7 3,921,225 - gidB b3740 0.23 -3.3 3,921,240 + gidB b3740 0.26 +0.9 3,921,252 - gidB b3740 0.28 +0.6 3,921,255 + gidB b3740 0.28 -1.3 3,921,263 - gidB b3740 0.29 +0.9 3,921,265 - gidB b3740 0.30 -1.9 3,921,310 - gidB b3740 0.37 +0.2 3,921,336 - gidB b3740 0.41 -2.5 3,921,336 - gidB b3740 0.41 +1.7 3,921,336 - gidB b3740 0.41 +2.9 3,921,338 + gidB b3740 0.41 +0.8 3,921,357 - gidB b3740 0.44 +3.3 3,921,390 - gidB b3740 0.50 -1.6 3,921,406 + gidB b3740 0.52 -0.9 3,921,406 + gidB b3740 0.52 -3.0 3,921,414 + gidB b3740 0.54 -3.0 3,921,414 + gidB b3740 0.54 -1.2 3,921,424 - gidB b3740 0.55 -0.5 3,921,424 - gidB b3740 0.55 -1.2 3,921,434 - gidB b3740 0.57 -2.1 3,921,434 - gidB b3740 0.57 -2.8 3,921,445 + gidB b3740 0.58 -1.8 3,921,450 + gidB b3740 0.59 -2.4
Or see this region's nucleotide sequence