Experiment: Tetrahymena sp., Mixed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pck and envZ are separated by 75 nucleotides envZ and ompR overlap by 4 nucleotides ompR and greB are separated by 227 nucleotides
b3403: pck - phosphoenolpyruvate carboxykinase (NCBI), at 3,530,840 to 3,532,462
pck
b3404: envZ - osmolarity sensor protein (NCBI), at 3,532,538 to 3,533,890
envZ
b3405: ompR - osmolarity response regulator (NCBI), at 3,533,887 to 3,534,606
ompR
b3406: greB - transcription elongation factor GreB (NCBI), at 3,534,834 to 3,535,310
greB
Position (kb)
3532
3533
3534 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1
2 at 3531.647 kb on + strand, within pck at 3531.662 kb on - strand, within pck at 3531.662 kb on - strand, within pck at 3531.747 kb on - strand, within pck at 3531.799 kb on - strand, within pck at 3531.840 kb on + strand, within pck at 3531.848 kb on - strand, within pck at 3531.868 kb on + strand, within pck at 3531.985 kb on + strand, within pck at 3532.017 kb on + strand, within pck at 3532.027 kb on + strand, within pck at 3532.050 kb on + strand, within pck at 3532.092 kb on + strand, within pck at 3532.092 kb on + strand, within pck at 3532.099 kb on + strand, within pck at 3532.122 kb on - strand, within pck at 3532.137 kb on - strand, within pck at 3532.144 kb on + strand, within pck at 3532.148 kb on - strand, within pck at 3532.153 kb on + strand, within pck at 3532.171 kb on - strand, within pck at 3532.178 kb on + strand, within pck at 3532.178 kb on + strand, within pck at 3532.225 kb on + strand, within pck at 3532.233 kb on - strand, within pck at 3532.235 kb on + strand, within pck at 3532.270 kb on - strand, within pck at 3532.270 kb on - strand, within pck at 3532.277 kb on - strand, within pck at 3532.277 kb on - strand, within pck at 3532.325 kb on - strand at 3532.325 kb on - strand at 3532.327 kb on + strand at 3532.329 kb on - strand at 3532.335 kb on - strand at 3532.335 kb on - strand at 3532.335 kb on - strand at 3532.411 kb on - strand at 3532.434 kb on + strand at 3532.434 kb on + strand at 3532.446 kb on - strand at 3532.559 kb on - strand at 3532.565 kb on - strand at 3532.630 kb on - strand at 3532.646 kb on - strand at 3532.646 kb on - strand at 3532.671 kb on - strand at 3532.671 kb on - strand at 3532.676 kb on + strand, within envZ at 3532.676 kb on + strand, within envZ at 3532.676 kb on + strand, within envZ at 3532.676 kb on + strand, within envZ at 3532.689 kb on + strand, within envZ at 3532.733 kb on - strand, within envZ at 3532.735 kb on + strand, within envZ at 3532.736 kb on + strand, within envZ at 3532.737 kb on + strand, within envZ at 3532.777 kb on - strand, within envZ at 3532.804 kb on - strand, within envZ at 3532.835 kb on + strand, within envZ at 3532.848 kb on - strand, within envZ at 3532.853 kb on + strand, within envZ at 3532.870 kb on - strand, within envZ at 3532.870 kb on - strand, within envZ at 3532.888 kb on - strand, within envZ at 3532.919 kb on + strand, within envZ at 3532.970 kb on - strand, within envZ at 3532.973 kb on + strand, within envZ at 3533.031 kb on - strand, within envZ at 3533.093 kb on + strand, within envZ at 3533.139 kb on - strand, within envZ at 3533.159 kb on + strand, within envZ at 3533.203 kb on + strand, within envZ at 3533.264 kb on + strand, within envZ at 3533.306 kb on + strand, within envZ at 3533.314 kb on - strand, within envZ at 3533.326 kb on - strand, within envZ at 3533.342 kb on + strand, within envZ at 3533.350 kb on - strand, within envZ at 3533.350 kb on - strand, within envZ at 3533.360 kb on - strand, within envZ at 3533.360 kb on - strand, within envZ at 3533.398 kb on + strand, within envZ at 3533.400 kb on + strand, within envZ at 3533.406 kb on - strand, within envZ at 3533.458 kb on + strand, within envZ at 3533.460 kb on + strand, within envZ at 3533.492 kb on + strand, within envZ at 3533.519 kb on + strand, within envZ at 3533.523 kb on - strand, within envZ at 3533.571 kb on - strand, within envZ at 3533.571 kb on - strand, within envZ at 3533.572 kb on - strand, within envZ at 3533.599 kb on - strand, within envZ at 3533.629 kb on - strand, within envZ at 3533.676 kb on + strand, within envZ at 3533.693 kb on + strand, within envZ at 3533.723 kb on + strand, within envZ at 3533.832 kb on + strand at 3533.853 kb on - strand at 3533.853 kb on - strand at 3533.871 kb on - strand at 3533.892 kb on + strand at 3533.900 kb on - strand at 3533.900 kb on - strand at 3533.900 kb on - strand at 3533.900 kb on - strand at 3533.907 kb on + strand at 3533.926 kb on + strand at 3533.933 kb on - strand at 3533.950 kb on - strand at 3534.010 kb on - strand, within ompR at 3534.010 kb on - strand, within ompR at 3534.057 kb on - strand, within ompR at 3534.093 kb on + strand, within ompR at 3534.097 kb on + strand, within ompR at 3534.097 kb on + strand, within ompR at 3534.097 kb on + strand, within ompR at 3534.105 kb on - strand, within ompR at 3534.105 kb on - strand, within ompR at 3534.129 kb on - strand, within ompR at 3534.168 kb on - strand, within ompR at 3534.206 kb on + strand, within ompR at 3534.228 kb on - strand, within ompR at 3534.257 kb on - strand, within ompR at 3534.260 kb on - strand, within ompR at 3534.267 kb on - strand, within ompR at 3534.267 kb on - strand, within ompR at 3534.358 kb on - strand, within ompR at 3534.436 kb on + strand, within ompR at 3534.447 kb on + strand, within ompR at 3534.456 kb on - strand, within ompR at 3534.509 kb on + strand, within ompR at 3534.514 kb on + strand, within ompR at 3534.514 kb on + strand, within ompR at 3534.522 kb on - strand, within ompR at 3534.541 kb on - strand at 3534.541 kb on - strand at 3534.544 kb on + strand at 3534.606 kb on - strand at 3534.608 kb on + strand at 3534.608 kb on + strand at 3534.616 kb on + strand at 3534.625 kb on - strand at 3534.632 kb on + strand at 3534.684 kb on - strand at 3534.724 kb on - strand at 3534.783 kb on - strand at 3534.851 kb on - strand at 3534.851 kb on - strand at 3534.852 kb on + strand at 3534.861 kb on - strand at 3534.861 kb on - strand at 3534.889 kb on + strand, within greB at 3534.889 kb on + strand, within greB
Per-strain Table
Position Strand Gene LocusTag Fraction Tetrahymena sp., Mixed culture-1 remove 3,531,647 + pck b3403 0.50 +0.7 3,531,662 - pck b3403 0.51 -0.6 3,531,662 - pck b3403 0.51 +1.1 3,531,747 - pck b3403 0.56 -2.7 3,531,799 - pck b3403 0.59 -0.5 3,531,840 + pck b3403 0.62 +1.0 3,531,848 - pck b3403 0.62 +1.0 3,531,868 + pck b3403 0.63 +1.0 3,531,985 + pck b3403 0.71 -0.1 3,532,017 + pck b3403 0.73 -0.2 3,532,027 + pck b3403 0.73 -3.6 3,532,050 + pck b3403 0.75 -3.1 3,532,092 + pck b3403 0.77 -1.3 3,532,092 + pck b3403 0.77 -3.7 3,532,099 + pck b3403 0.78 -2.7 3,532,122 - pck b3403 0.79 +1.2 3,532,137 - pck b3403 0.80 +1.0 3,532,144 + pck b3403 0.80 -4.3 3,532,148 - pck b3403 0.81 +0.3 3,532,153 + pck b3403 0.81 -3.5 3,532,171 - pck b3403 0.82 -1.1 3,532,178 + pck b3403 0.82 +1.9 3,532,178 + pck b3403 0.82 +2.5 3,532,225 + pck b3403 0.85 -1.0 3,532,233 - pck b3403 0.86 +1.1 3,532,235 + pck b3403 0.86 -0.9 3,532,270 - pck b3403 0.88 +1.7 3,532,270 - pck b3403 0.88 -3.4 3,532,277 - pck b3403 0.89 +1.1 3,532,277 - pck b3403 0.89 -0.3 3,532,325 - -4.2 3,532,325 - +2.4 3,532,327 + -0.3 3,532,329 - -0.5 3,532,335 - +2.7 3,532,335 - -4.0 3,532,335 - -0.1 3,532,411 - -3.2 3,532,434 + +0.8 3,532,434 + -2.4 3,532,446 - -0.6 3,532,559 - -3.7 3,532,565 - +0.8 3,532,630 - -1.0 3,532,646 - -3.6 3,532,646 - -3.4 3,532,671 - -3.8 3,532,671 - -1.7 3,532,676 + envZ b3404 0.10 -1.6 3,532,676 + envZ b3404 0.10 -1.9 3,532,676 + envZ b3404 0.10 -2.1 3,532,676 + envZ b3404 0.10 -2.9 3,532,689 + envZ b3404 0.11 -4.5 3,532,733 - envZ b3404 0.14 -3.2 3,532,735 + envZ b3404 0.15 -4.2 3,532,736 + envZ b3404 0.15 -4.9 3,532,737 + envZ b3404 0.15 -2.0 3,532,777 - envZ b3404 0.18 -3.8 3,532,804 - envZ b3404 0.20 -4.2 3,532,835 + envZ b3404 0.22 -1.2 3,532,848 - envZ b3404 0.23 -2.7 3,532,853 + envZ b3404 0.23 -3.9 3,532,870 - envZ b3404 0.25 -2.4 3,532,870 - envZ b3404 0.25 -3.1 3,532,888 - envZ b3404 0.26 -2.1 3,532,919 + envZ b3404 0.28 -3.0 3,532,970 - envZ b3404 0.32 -3.8 3,532,973 + envZ b3404 0.32 -4.9 3,533,031 - envZ b3404 0.36 -3.2 3,533,093 + envZ b3404 0.41 -5.5 3,533,139 - envZ b3404 0.44 -5.0 3,533,159 + envZ b3404 0.46 -1.9 3,533,203 + envZ b3404 0.49 -5.9 3,533,264 + envZ b3404 0.54 -6.9 3,533,306 + envZ b3404 0.57 -3.6 3,533,314 - envZ b3404 0.57 -3.8 3,533,326 - envZ b3404 0.58 -5.0 3,533,342 + envZ b3404 0.59 -4.5 3,533,350 - envZ b3404 0.60 +0.9 3,533,350 - envZ b3404 0.60 -2.7 3,533,360 - envZ b3404 0.61 -4.8 3,533,360 - envZ b3404 0.61 -4.6 3,533,398 + envZ b3404 0.64 -4.2 3,533,400 + envZ b3404 0.64 -3.5 3,533,406 - envZ b3404 0.64 -4.3 3,533,458 + envZ b3404 0.68 -1.7 3,533,460 + envZ b3404 0.68 -5.6 3,533,492 + envZ b3404 0.71 -4.0 3,533,519 + envZ b3404 0.73 -3.6 3,533,523 - envZ b3404 0.73 -4.3 3,533,571 - envZ b3404 0.76 -5.7 3,533,571 - envZ b3404 0.76 -5.8 3,533,572 - envZ b3404 0.76 -5.9 3,533,599 - envZ b3404 0.78 -4.1 3,533,629 - envZ b3404 0.81 -0.3 3,533,676 + envZ b3404 0.84 -3.3 3,533,693 + envZ b3404 0.85 -2.0 3,533,723 + envZ b3404 0.88 -5.5 3,533,832 + +1.0 3,533,853 - -1.2 3,533,853 - -2.2 3,533,871 - -4.2 3,533,892 + -4.4 3,533,900 - -2.1 3,533,900 - +0.8 3,533,900 - -3.9 3,533,900 - -2.9 3,533,907 + -3.9 3,533,926 + -2.9 3,533,933 - -1.0 3,533,950 - -2.2 3,534,010 - ompR b3405 0.17 -2.9 3,534,010 - ompR b3405 0.17 -2.1 3,534,057 - ompR b3405 0.24 -1.0 3,534,093 + ompR b3405 0.29 -3.9 3,534,097 + ompR b3405 0.29 -5.6 3,534,097 + ompR b3405 0.29 -1.4 3,534,097 + ompR b3405 0.29 -3.6 3,534,105 - ompR b3405 0.30 -3.6 3,534,105 - ompR b3405 0.30 -3.2 3,534,129 - ompR b3405 0.34 -3.6 3,534,168 - ompR b3405 0.39 -3.5 3,534,206 + ompR b3405 0.44 -3.1 3,534,228 - ompR b3405 0.47 -1.2 3,534,257 - ompR b3405 0.51 -3.5 3,534,260 - ompR b3405 0.52 -1.6 3,534,267 - ompR b3405 0.53 -2.2 3,534,267 - ompR b3405 0.53 -1.6 3,534,358 - ompR b3405 0.65 -3.6 3,534,436 + ompR b3405 0.76 -2.5 3,534,447 + ompR b3405 0.78 -1.8 3,534,456 - ompR b3405 0.79 -3.8 3,534,509 + ompR b3405 0.86 -3.8 3,534,514 + ompR b3405 0.87 -1.2 3,534,514 + ompR b3405 0.87 -1.7 3,534,522 - ompR b3405 0.88 -4.2 3,534,541 - -1.2 3,534,541 - -1.3 3,534,544 + +1.0 3,534,606 - -4.5 3,534,608 + -4.1 3,534,608 + -2.7 3,534,616 + +0.9 3,534,625 - -0.7 3,534,632 + -3.3 3,534,684 - -1.5 3,534,724 - -3.4 3,534,783 - +2.1 3,534,851 - -1.4 3,534,851 - +1.6 3,534,852 + -3.3 3,534,861 - -3.9 3,534,861 - -1.5 3,534,889 + greB b3406 0.12 -2.7 3,534,889 + greB b3406 0.12 -2.0
Or see this region's nucleotide sequence