Experiment: Tetrahymena sp., Mixed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt motA and flhC are separated by 126 nucleotides flhC and flhD are separated by 2 nucleotides flhD and insB-5 are separated by 320 nucleotides insB-5 and insA-5 overlap by 82 nucleotides
b1890: motA - flagellar motor protein MotA (NCBI), at 1,974,276 to 1,975,163
motA
b1891: flhC - DNA-binding transcriptional dual regulator with FlhD (NCBI), at 1,975,290 to 1,975,868
flhC
b1892: flhD - DNA-binding transcriptional dual regulator with FlhC (RefSeq), at 1,975,871 to 1,976,221
flhD
b1893: insB-5 - IS1 transposase InsAB' (NCBI), at 1,976,542 to 1,977,045
insB-5
b1894: insA-5 - IS1 repressor protein InsA (NCBI), at 1,976,964 to 1,977,239
insA-5
Position (kb)
1975
1976
1977 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1974.934 kb on + strand, within motA at 1974.934 kb on + strand, within motA at 1975.040 kb on + strand, within motA at 1975.092 kb on + strand at 1975.093 kb on - strand at 1975.173 kb on + strand at 1975.179 kb on + strand at 1975.179 kb on + strand at 1975.415 kb on - strand, within flhC at 1975.477 kb on + strand, within flhC at 1975.589 kb on + strand, within flhC at 1975.636 kb on + strand, within flhC at 1975.707 kb on + strand, within flhC at 1975.914 kb on - strand, within flhD at 1975.961 kb on - strand, within flhD at 1975.961 kb on - strand, within flhD at 1975.991 kb on + strand, within flhD at 1975.991 kb on - strand, within flhD at 1976.072 kb on + strand, within flhD at 1976.072 kb on + strand, within flhD at 1976.072 kb on + strand, within flhD at 1976.120 kb on + strand, within flhD at 1976.120 kb on + strand, within flhD at 1976.138 kb on + strand, within flhD at 1976.146 kb on - strand, within flhD at 1976.149 kb on - strand, within flhD at 1976.149 kb on - strand, within flhD at 1976.221 kb on - strand at 1976.226 kb on - strand at 1976.226 kb on - strand at 1976.259 kb on + strand at 1976.262 kb on + strand at 1976.272 kb on + strand at 1976.281 kb on + strand at 1976.354 kb on - strand at 1976.387 kb on + strand at 1976.396 kb on - strand at 1976.432 kb on - strand at 1976.436 kb on + strand at 1976.439 kb on + strand at 1976.439 kb on + strand at 1976.439 kb on + strand at 1976.439 kb on + strand at 1976.444 kb on + strand at 1976.447 kb on - strand at 1976.447 kb on - strand at 1976.447 kb on - strand at 1976.447 kb on - strand at 1976.448 kb on + strand at 1976.452 kb on - strand at 1976.452 kb on - strand at 1976.453 kb on + strand at 1976.454 kb on - strand at 1976.455 kb on - strand at 1976.460 kb on - strand at 1976.514 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetrahymena sp., Mixed culture-1 remove 1,974,934 + motA b1890 0.74 +0.1 1,974,934 + motA b1890 0.74 -0.2 1,975,040 + motA b1890 0.86 -0.3 1,975,092 + +2.2 1,975,093 - -1.6 1,975,173 + -0.4 1,975,179 + +1.5 1,975,179 + -0.5 1,975,415 - flhC b1891 0.22 +2.3 1,975,477 + flhC b1891 0.32 -1.6 1,975,589 + flhC b1891 0.52 +1.9 1,975,636 + flhC b1891 0.60 +1.7 1,975,707 + flhC b1891 0.72 +1.6 1,975,914 - flhD b1892 0.12 -1.1 1,975,961 - flhD b1892 0.26 +0.7 1,975,961 - flhD b1892 0.26 -0.6 1,975,991 + flhD b1892 0.34 +0.3 1,975,991 - flhD b1892 0.34 +1.6 1,976,072 + flhD b1892 0.57 +4.5 1,976,072 + flhD b1892 0.57 -0.8 1,976,072 + flhD b1892 0.57 +1.3 1,976,120 + flhD b1892 0.71 -2.0 1,976,120 + flhD b1892 0.71 -3.2 1,976,138 + flhD b1892 0.76 +3.5 1,976,146 - flhD b1892 0.78 +2.6 1,976,149 - flhD b1892 0.79 +2.3 1,976,149 - flhD b1892 0.79 +3.6 1,976,221 - -3.3 1,976,226 - -2.3 1,976,226 - +0.2 1,976,259 + +1.2 1,976,262 + +4.8 1,976,272 + +2.8 1,976,281 + +1.8 1,976,354 - -0.8 1,976,387 + +1.2 1,976,396 - -2.6 1,976,432 - -2.3 1,976,436 + +1.8 1,976,439 + +2.0 1,976,439 + +2.8 1,976,439 + +0.1 1,976,439 + +0.9 1,976,444 + +1.9 1,976,447 - -3.0 1,976,447 - -1.6 1,976,447 - -4.5 1,976,447 - -1.1 1,976,448 + -1.3 1,976,452 - -1.5 1,976,452 - -2.3 1,976,453 + +0.6 1,976,454 - -2.0 1,976,455 - -2.7 1,976,460 - -3.0 1,976,514 - -1.9
Or see this region's nucleotide sequence