Experiment: Tetrahymena sp., Mixed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt appB and yccB are separated by 12 nucleotides yccB and appA are separated by 79 nucleotides appA and yccC are separated by 114 nucleotides
b0979: appB - cytochrome bd-II oxidase, subunit II (NCBI), at 1,038,519 to 1,039,655
appB
b4592: yccB - hypothetical protein (RefSeq), at 1,039,668 to 1,039,760
yccB
b0980: appA - phosphoanhydride phosphorylase (NCBI), at 1,039,840 to 1,041,138
appA
b0981: yccC - cryptic autophosphorylating protein tyrosine kinase Etk (NCBI), at 1,041,253 to 1,043,433
yccC
Position (kb)
1039
1040
1041
1042 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1039.201 kb on + strand, within appB at 1039.201 kb on + strand, within appB at 1039.201 kb on + strand, within appB at 1039.209 kb on - strand, within appB at 1039.209 kb on - strand, within appB at 1039.263 kb on + strand, within appB at 1039.453 kb on - strand, within appB at 1039.453 kb on - strand, within appB at 1039.528 kb on + strand, within appB at 1039.566 kb on + strand at 1039.581 kb on + strand at 1039.617 kb on + strand at 1039.728 kb on - strand, within yccB at 1039.730 kb on + strand, within yccB at 1039.730 kb on + strand, within yccB at 1039.764 kb on + strand at 1039.829 kb on + strand at 1039.878 kb on - strand at 1039.903 kb on + strand at 1039.903 kb on + strand at 1039.916 kb on - strand at 1039.950 kb on + strand at 1039.950 kb on + strand at 1039.950 kb on + strand at 1039.960 kb on - strand at 1040.065 kb on - strand, within appA at 1040.184 kb on - strand, within appA at 1040.226 kb on - strand, within appA at 1040.254 kb on + strand, within appA at 1040.348 kb on - strand, within appA at 1040.782 kb on - strand, within appA at 1040.797 kb on - strand, within appA at 1040.816 kb on + strand, within appA at 1040.816 kb on + strand, within appA at 1040.822 kb on - strand, within appA at 1040.822 kb on - strand, within appA at 1040.874 kb on + strand, within appA at 1040.894 kb on + strand, within appA at 1040.894 kb on + strand, within appA at 1041.148 kb on - strand at 1041.148 kb on - strand at 1041.171 kb on + strand at 1041.197 kb on - strand at 1041.197 kb on - strand at 1041.272 kb on + strand at 1041.286 kb on - strand at 1041.331 kb on + strand at 1041.331 kb on + strand at 1041.339 kb on - strand at 1041.360 kb on - strand at 1041.366 kb on + strand at 1041.419 kb on - strand at 1041.554 kb on + strand, within yccC at 1041.554 kb on + strand, within yccC at 1041.573 kb on + strand, within yccC at 1041.573 kb on + strand, within yccC at 1041.695 kb on + strand, within yccC at 1042.035 kb on - strand, within yccC at 1042.035 kb on - strand, within yccC at 1042.053 kb on + strand, within yccC
Per-strain Table
Position Strand Gene LocusTag Fraction Tetrahymena sp., Mixed culture-1 remove 1,039,201 + appB b0979 0.60 -0.2 1,039,201 + appB b0979 0.60 +0.3 1,039,201 + appB b0979 0.60 +0.5 1,039,209 - appB b0979 0.61 +0.0 1,039,209 - appB b0979 0.61 -1.5 1,039,263 + appB b0979 0.65 -1.2 1,039,453 - appB b0979 0.82 +1.5 1,039,453 - appB b0979 0.82 -1.5 1,039,528 + appB b0979 0.89 -2.6 1,039,566 + +1.7 1,039,581 + +0.7 1,039,617 + -3.1 1,039,728 - yccB b4592 0.65 +3.3 1,039,730 + yccB b4592 0.67 -1.7 1,039,730 + yccB b4592 0.67 +1.8 1,039,764 + +1.7 1,039,829 + +1.5 1,039,878 - -0.8 1,039,903 + -4.1 1,039,903 + -2.3 1,039,916 - +0.9 1,039,950 + +0.9 1,039,950 + -1.1 1,039,950 + +1.7 1,039,960 - +0.9 1,040,065 - appA b0980 0.17 -0.2 1,040,184 - appA b0980 0.26 +2.8 1,040,226 - appA b0980 0.30 +0.1 1,040,254 + appA b0980 0.32 -0.5 1,040,348 - appA b0980 0.39 +0.1 1,040,782 - appA b0980 0.73 +1.9 1,040,797 - appA b0980 0.74 -1.0 1,040,816 + appA b0980 0.75 +1.5 1,040,816 + appA b0980 0.75 +2.6 1,040,822 - appA b0980 0.76 -2.9 1,040,822 - appA b0980 0.76 +2.2 1,040,874 + appA b0980 0.80 +0.4 1,040,894 + appA b0980 0.81 -2.2 1,040,894 + appA b0980 0.81 +2.4 1,041,148 - +1.9 1,041,148 - -2.8 1,041,171 + -3.5 1,041,197 - -0.7 1,041,197 - -1.4 1,041,272 + -4.7 1,041,286 - -2.3 1,041,331 + +0.5 1,041,331 + +0.8 1,041,339 - +1.3 1,041,360 - +1.1 1,041,366 + -2.4 1,041,419 - +0.9 1,041,554 + yccC b0981 0.14 +3.0 1,041,554 + yccC b0981 0.14 +1.7 1,041,573 + yccC b0981 0.15 +1.5 1,041,573 + yccC b0981 0.15 -2.7 1,041,695 + yccC b0981 0.20 -3.8 1,042,035 - yccC b0981 0.36 +2.1 1,042,035 - yccC b0981 0.36 +0.1 1,042,053 + yccC b0981 0.37 -2.1
Or see this region's nucleotide sequence