Experiment: Tetrahymena sp., Mixed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b0878 and macB overlap by 4 nucleotides macB and cspD are separated by 72 nucleotides cspD and clpS are separated by 322 nucleotides clpS and clpA are separated by 30 nucleotides
b0878: b0878 - putative membrane protein (VIMSS), at 918,458 to 919,573
b0878
b0879: macB - fused macrolide transporter subunits of ABC superfamily: ATP-binding component/membrane component (NCBI), at 919,570 to 921,516
macB
b0880: cspD - cold shock protein homolog (NCBI), at 921,589 to 921,813
cspD
b0881: clpS - ATP-dependent Clp protease adaptor protein ClpS (NCBI), at 922,136 to 922,456
clpS
b0882: clpA - ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease, chaperone activity (NCBI), at 922,487 to 924,763
clpA
Position (kb) 919
920
921
922 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 918.620 kb on + strand, within b0878 at 918.789 kb on - strand, within b0878 at 918.849 kb on - strand at 918.849 kb on - strand, within b0878 at 918.985 kb on + strand, within b0878 at 919.227 kb on - strand, within b0878 at 919.262 kb on + strand, within b0878 at 919.262 kb on + strand, within b0878 at 919.298 kb on - strand, within b0878 at 919.298 kb on - strand, within b0878 at 919.343 kb on + strand, within b0878 at 919.557 kb on - strand at 919.636 kb on + strand at 919.765 kb on + strand, within macB at 919.979 kb on + strand, within macB at 919.979 kb on + strand, within macB at 919.994 kb on - strand, within macB at 919.994 kb on - strand, within macB at 920.012 kb on + strand at 920.023 kb on + strand, within macB at 920.068 kb on - strand, within macB at 920.199 kb on - strand, within macB at 920.267 kb on + strand, within macB at 920.415 kb on - strand, within macB at 920.443 kb on + strand, within macB at 920.458 kb on - strand, within macB at 920.459 kb on + strand, within macB at 920.459 kb on + strand, within macB at 920.712 kb on - strand, within macB at 920.767 kb on - strand, within macB at 920.849 kb on + strand, within macB at 920.979 kb on + strand, within macB at 921.004 kb on + strand, within macB at 921.164 kb on + strand, within macB at 921.169 kb on + strand, within macB at 921.254 kb on - strand, within macB at 921.254 kb on - strand, within macB at 921.271 kb on + strand, within macB at 921.298 kb on + strand, within macB at 921.298 kb on + strand, within macB at 921.790 kb on + strand, within cspD at 921.790 kb on + strand, within cspD at 921.956 kb on - strand at 921.956 kb on - strand at 922.042 kb on + strand at 922.042 kb on + strand at 922.055 kb on + strand at 922.069 kb on + strand at 922.091 kb on - strand at 922.103 kb on + strand at 922.124 kb on + strand at 922.246 kb on - strand, within clpS at 922.417 kb on + strand, within clpS at 922.435 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetrahymena sp., Mixed culture-1 remove 918,620 + b0878 b0878 0.15 +1.7 918,789 - b0878 b0878 0.30 -3.9 918,849 - -1.4 918,849 - b0878 b0878 0.35 +0.1 918,985 + b0878 b0878 0.47 +1.6 919,227 - b0878 b0878 0.69 +3.0 919,262 + b0878 b0878 0.72 -4.6 919,262 + b0878 b0878 0.72 +1.5 919,298 - b0878 b0878 0.75 +0.3 919,298 - b0878 b0878 0.75 -0.9 919,343 + b0878 b0878 0.79 -1.5 919,557 - -1.2 919,636 + -1.2 919,765 + macB b0879 0.10 +2.3 919,979 + macB b0879 0.21 +0.6 919,979 + macB b0879 0.21 +1.3 919,994 - macB b0879 0.22 -2.6 919,994 - macB b0879 0.22 -0.4 920,012 + -0.7 920,023 + macB b0879 0.23 -1.1 920,068 - macB b0879 0.26 +1.6 920,199 - macB b0879 0.32 +0.0 920,267 + macB b0879 0.36 +2.1 920,415 - macB b0879 0.43 +0.9 920,443 + macB b0879 0.45 +0.5 920,458 - macB b0879 0.46 +0.8 920,459 + macB b0879 0.46 -0.6 920,459 + macB b0879 0.46 +1.1 920,712 - macB b0879 0.59 -0.1 920,767 - macB b0879 0.61 -1.5 920,849 + macB b0879 0.66 -2.9 920,979 + macB b0879 0.72 -0.8 921,004 + macB b0879 0.74 +0.6 921,164 + macB b0879 0.82 -3.8 921,169 + macB b0879 0.82 -4.8 921,254 - macB b0879 0.86 +1.8 921,254 - macB b0879 0.86 +2.1 921,271 + macB b0879 0.87 -2.9 921,298 + macB b0879 0.89 -3.3 921,298 + macB b0879 0.89 -2.9 921,790 + cspD b0880 0.89 -4.4 921,790 + cspD b0880 0.89 -4.1 921,956 - +0.5 921,956 - -0.5 922,042 + +0.5 922,042 + +0.4 922,055 + +2.0 922,069 + -0.1 922,091 - -1.6 922,103 + -3.4 922,124 + -5.1 922,246 - clpS b0881 0.34 +1.7 922,417 + clpS b0881 0.88 +1.3 922,435 - -2.5
Or see this region's nucleotide sequence