Experiment: Tetrahymena sp., Mixed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sucC and sucD overlap by 1 nucleotides sucD and mngR are separated by 103 nucleotides mngR and mngA are separated by 108 nucleotides
b0728: sucC - succinyl-CoA synthetase subunit beta (NCBI), at 762,237 to 763,403
sucC
b0729: sucD - succinyl-CoA synthetase subunit alpha (NCBI), at 763,403 to 764,272
sucD
b0730: mngR - DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI), at 764,376 to 765,098
mngR
b0731: mngA - fused 2-O-a-mannosyl-D-glycerate specific PTS enzymes: IIA component/IIB component/IIC component (NCBI), at 765,207 to 767,183
mngA
Position (kb) 764
765
766 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 763.625 kb on + strand, within sucD at 763.625 kb on + strand, within sucD at 763.712 kb on + strand, within sucD at 763.712 kb on + strand, within sucD at 763.773 kb on + strand, within sucD at 763.871 kb on - strand, within sucD at 763.901 kb on + strand, within sucD at 764.252 kb on - strand at 764.279 kb on + strand at 764.302 kb on + strand at 764.302 kb on + strand at 764.311 kb on + strand at 764.319 kb on + strand at 764.349 kb on + strand at 764.480 kb on + strand, within mngR at 764.480 kb on + strand, within mngR at 764.515 kb on + strand, within mngR at 764.515 kb on + strand, within mngR at 764.515 kb on + strand, within mngR at 764.515 kb on + strand, within mngR at 764.645 kb on + strand, within mngR at 764.668 kb on + strand, within mngR at 764.811 kb on + strand, within mngR at 765.279 kb on + strand at 765.424 kb on + strand, within mngA at 765.590 kb on + strand, within mngA at 765.590 kb on + strand, within mngA at 765.674 kb on + strand, within mngA at 765.674 kb on - strand, within mngA at 765.675 kb on - strand, within mngA at 765.803 kb on - strand, within mngA at 765.803 kb on - strand, within mngA at 765.811 kb on - strand, within mngA at 765.811 kb on - strand, within mngA at 765.960 kb on - strand, within mngA at 766.059 kb on - strand, within mngA at 766.059 kb on - strand, within mngA at 766.066 kb on - strand, within mngA
Per-strain Table
Position Strand Gene LocusTag Fraction Tetrahymena sp., Mixed culture-1 remove 763,625 + sucD b0729 0.26 -1.6 763,625 + sucD b0729 0.26 +0.2 763,712 + sucD b0729 0.36 -1.9 763,712 + sucD b0729 0.36 +0.8 763,773 + sucD b0729 0.43 -0.9 763,871 - sucD b0729 0.54 -3.4 763,901 + sucD b0729 0.57 -3.5 764,252 - +1.0 764,279 + +0.7 764,302 + -0.6 764,302 + +2.3 764,311 + +1.0 764,319 + -1.0 764,349 + -1.2 764,480 + mngR b0730 0.14 +0.2 764,480 + mngR b0730 0.14 -1.4 764,515 + mngR b0730 0.19 -1.1 764,515 + mngR b0730 0.19 -0.8 764,515 + mngR b0730 0.19 -0.8 764,515 + mngR b0730 0.19 +1.2 764,645 + mngR b0730 0.37 +0.2 764,668 + mngR b0730 0.40 -1.5 764,811 + mngR b0730 0.60 +0.6 765,279 + +2.3 765,424 + mngA b0731 0.11 -3.8 765,590 + mngA b0731 0.19 +0.8 765,590 + mngA b0731 0.19 +3.0 765,674 + mngA b0731 0.24 +0.6 765,674 - mngA b0731 0.24 -3.5 765,675 - mngA b0731 0.24 +1.6 765,803 - mngA b0731 0.30 +0.4 765,803 - mngA b0731 0.30 -1.0 765,811 - mngA b0731 0.31 -1.2 765,811 - mngA b0731 0.31 -3.3 765,960 - mngA b0731 0.38 -2.3 766,059 - mngA b0731 0.43 -2.0 766,059 - mngA b0731 0.43 +1.4 766,066 - mngA b0731 0.43 +2.1
Or see this region's nucleotide sequence