Experiment: Tetrahymena sp., Mixed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt kdpB and kdpA are separated by 22 nucleotides kdpA and kdpF overlap by 1 nucleotides kdpF and ybfA are separated by 312 nucleotides ybfA and rhsC are separated by 242 nucleotides
b0697: kdpB - potassium-transporting ATPase subunit B (NCBI), at 724,211 to 726,259
kdpB
b0698: kdpA - potassium-transporting ATPase subunit A (NCBI), at 726,282 to 727,955
kdpA
b4513: kdpF - potassium ion accessory transporter subunit (NCBI), at 727,955 to 728,044
kdpF
b0699: ybfA - hypothetical protein (NCBI), at 728,357 to 728,563
ybfA
b0700: rhsC - rhsC element core protein RshC (NCBI), at 728,806 to 732,999
rhsC
Position (kb) 726
727
728 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 725.492 kb on + strand, within kdpB at 725.520 kb on - strand, within kdpB at 725.553 kb on - strand, within kdpB at 725.553 kb on - strand, within kdpB at 725.667 kb on + strand, within kdpB at 725.705 kb on + strand, within kdpB at 725.778 kb on - strand, within kdpB at 725.839 kb on + strand, within kdpB at 725.871 kb on - strand, within kdpB at 725.966 kb on + strand, within kdpB at 725.974 kb on - strand, within kdpB at 726.039 kb on - strand, within kdpB at 726.117 kb on - strand at 726.247 kb on - strand at 726.247 kb on - strand at 726.263 kb on - strand at 726.331 kb on - strand at 726.331 kb on - strand at 726.353 kb on - strand at 726.388 kb on + strand at 726.435 kb on - strand at 726.561 kb on - strand, within kdpA at 726.580 kb on + strand, within kdpA at 726.590 kb on - strand, within kdpA at 726.590 kb on - strand, within kdpA at 726.752 kb on + strand, within kdpA at 726.945 kb on - strand, within kdpA at 726.981 kb on + strand, within kdpA at 727.062 kb on - strand, within kdpA at 727.075 kb on - strand, within kdpA at 727.075 kb on - strand, within kdpA at 727.107 kb on - strand, within kdpA at 727.325 kb on - strand, within kdpA at 727.338 kb on + strand, within kdpA at 727.338 kb on + strand, within kdpA at 727.435 kb on - strand, within kdpA at 727.464 kb on + strand, within kdpA at 727.600 kb on + strand, within kdpA at 727.600 kb on + strand, within kdpA at 727.640 kb on - strand, within kdpA at 727.687 kb on - strand, within kdpA at 727.692 kb on - strand, within kdpA at 727.696 kb on + strand, within kdpA at 727.696 kb on + strand, within kdpA at 727.696 kb on + strand, within kdpA at 727.767 kb on + strand, within kdpA at 727.792 kb on + strand at 727.871 kb on + strand at 727.871 kb on + strand at 727.884 kb on + strand at 727.904 kb on - strand at 727.904 kb on - strand at 727.963 kb on + strand at 727.966 kb on - strand at 727.988 kb on - strand, within kdpF at 728.022 kb on - strand, within kdpF at 728.054 kb on - strand at 728.054 kb on - strand at 728.059 kb on - strand at 728.068 kb on - strand at 728.076 kb on - strand at 728.580 kb on + strand at 728.597 kb on + strand at 728.641 kb on + strand at 728.641 kb on + strand at 728.758 kb on - strand at 728.810 kb on - strand at 728.840 kb on - strand at 728.863 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetrahymena sp., Mixed culture-1 remove 725,492 + kdpB b0697 0.63 +0.8 725,520 - kdpB b0697 0.64 -4.5 725,553 - kdpB b0697 0.65 +1.0 725,553 - kdpB b0697 0.65 +2.3 725,667 + kdpB b0697 0.71 +0.2 725,705 + kdpB b0697 0.73 +1.3 725,778 - kdpB b0697 0.76 -4.1 725,839 + kdpB b0697 0.79 +0.8 725,871 - kdpB b0697 0.81 -1.3 725,966 + kdpB b0697 0.86 -0.3 725,974 - kdpB b0697 0.86 -4.7 726,039 - kdpB b0697 0.89 -2.0 726,117 - +1.8 726,247 - -4.6 726,247 - +0.8 726,263 - +0.2 726,331 - -0.0 726,331 - -0.5 726,353 - +1.0 726,388 + +1.8 726,435 - +1.2 726,561 - kdpA b0698 0.17 +1.0 726,580 + kdpA b0698 0.18 +0.2 726,590 - kdpA b0698 0.18 -4.0 726,590 - kdpA b0698 0.18 +0.4 726,752 + kdpA b0698 0.28 -1.7 726,945 - kdpA b0698 0.40 -0.1 726,981 + kdpA b0698 0.42 -0.4 727,062 - kdpA b0698 0.47 -1.0 727,075 - kdpA b0698 0.47 -3.1 727,075 - kdpA b0698 0.47 -0.6 727,107 - kdpA b0698 0.49 -0.2 727,325 - kdpA b0698 0.62 +0.7 727,338 + kdpA b0698 0.63 -2.6 727,338 + kdpA b0698 0.63 -3.7 727,435 - kdpA b0698 0.69 -0.9 727,464 + kdpA b0698 0.71 -4.9 727,600 + kdpA b0698 0.79 +1.1 727,600 + kdpA b0698 0.79 +0.5 727,640 - kdpA b0698 0.81 -2.7 727,687 - kdpA b0698 0.84 -0.3 727,692 - kdpA b0698 0.84 +0.5 727,696 + kdpA b0698 0.84 -2.5 727,696 + kdpA b0698 0.84 +2.5 727,696 + kdpA b0698 0.84 -2.7 727,767 + kdpA b0698 0.89 -2.6 727,792 + +0.0 727,871 + -1.0 727,871 + +0.4 727,884 + -0.2 727,904 - +0.9 727,904 - +0.4 727,963 + +0.5 727,966 - +4.1 727,988 - kdpF b4513 0.37 -0.4 728,022 - kdpF b4513 0.74 +1.3 728,054 - +0.9 728,054 - -0.8 728,059 - -0.4 728,068 - +2.9 728,076 - +1.6 728,580 + -1.2 728,597 + -5.3 728,641 + +0.4 728,641 + -0.1 728,758 - +2.0 728,810 - -1.3 728,840 - -1.6 728,863 - -2.1
Or see this region's nucleotide sequence