Experiment: Tetrahymena sp., Mixed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glnS and ybfM are separated by 576 nucleotides ybfM and ybfN are separated by 49 nucleotides ybfN and fur are separated by 83 nucleotides fur and uof overlap by 8 nucleotides
b0680: glnS - glutaminyl-tRNA synthetase (NCBI), at 705,316 to 706,980
glnS
b0681: ybfM - predicted outer membrane porin (NCBI), at 707,557 to 708,963
ybfM
b0682: ybfN - predicted lipoprotein (NCBI), at 709,013 to 709,339
ybfN
b0683: fur - ferric uptake regulator (NCBI), at 709,423 to 709,869
fur
b4637: uof - ryhB-regulated fur leader peptide (RefSeq), at 709,862 to 709,948
uof
Position (kb) 707
708
709 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 706.950 kb on + strand at 706.950 kb on + strand at 707.325 kb on - strand at 707.325 kb on - strand at 707.356 kb on - strand at 707.356 kb on - strand at 707.456 kb on + strand at 707.546 kb on - strand at 707.566 kb on - strand at 707.566 kb on - strand at 707.664 kb on - strand at 707.834 kb on + strand, within ybfM at 707.917 kb on + strand, within ybfM at 707.945 kb on + strand, within ybfM at 707.983 kb on + strand, within ybfM at 708.126 kb on + strand, within ybfM at 708.126 kb on + strand, within ybfM at 708.224 kb on + strand, within ybfM at 708.279 kb on - strand, within ybfM at 708.288 kb on + strand, within ybfM at 708.288 kb on + strand, within ybfM at 708.288 kb on + strand, within ybfM at 708.288 kb on + strand, within ybfM at 708.291 kb on + strand, within ybfM at 708.389 kb on - strand, within ybfM at 708.389 kb on - strand, within ybfM at 708.760 kb on - strand, within ybfM at 708.764 kb on + strand, within ybfM at 708.766 kb on + strand, within ybfM at 708.766 kb on + strand, within ybfM at 708.785 kb on + strand, within ybfM at 708.785 kb on + strand, within ybfM at 708.800 kb on + strand, within ybfM at 708.803 kb on + strand, within ybfM at 708.894 kb on - strand at 708.943 kb on + strand at 709.039 kb on + strand at 709.113 kb on - strand, within ybfN at 709.113 kb on - strand, within ybfN at 709.126 kb on - strand, within ybfN at 709.238 kb on + strand, within ybfN at 709.247 kb on - strand, within ybfN at 709.247 kb on - strand, within ybfN at 709.257 kb on - strand, within ybfN at 709.428 kb on - strand at 709.428 kb on - strand at 709.595 kb on + strand, within fur at 709.678 kb on - strand, within fur at 709.699 kb on + strand, within fur at 709.861 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetrahymena sp., Mixed culture-1 remove 706,950 + +2.2 706,950 + -0.2 707,325 - +0.8 707,325 - +2.1 707,356 - +0.4 707,356 - +1.8 707,456 + +0.4 707,546 - +2.3 707,566 - +0.9 707,566 - +0.6 707,664 - -2.3 707,834 + ybfM b0681 0.20 +2.4 707,917 + ybfM b0681 0.26 +0.4 707,945 + ybfM b0681 0.28 -2.9 707,983 + ybfM b0681 0.30 -3.8 708,126 + ybfM b0681 0.40 -1.8 708,126 + ybfM b0681 0.40 +0.2 708,224 + ybfM b0681 0.47 -3.6 708,279 - ybfM b0681 0.51 -0.7 708,288 + ybfM b0681 0.52 +0.2 708,288 + ybfM b0681 0.52 -1.4 708,288 + ybfM b0681 0.52 -2.6 708,288 + ybfM b0681 0.52 +0.8 708,291 + ybfM b0681 0.52 +1.2 708,389 - ybfM b0681 0.59 -0.2 708,389 - ybfM b0681 0.59 -0.9 708,760 - ybfM b0681 0.86 -4.3 708,764 + ybfM b0681 0.86 -1.4 708,766 + ybfM b0681 0.86 +0.5 708,766 + ybfM b0681 0.86 +0.2 708,785 + ybfM b0681 0.87 -3.4 708,785 + ybfM b0681 0.87 +0.2 708,800 + ybfM b0681 0.88 +0.9 708,803 + ybfM b0681 0.89 -2.3 708,894 - -4.5 708,943 + -2.9 709,039 + +0.2 709,113 - ybfN b0682 0.31 +0.4 709,113 - ybfN b0682 0.31 -0.3 709,126 - ybfN b0682 0.35 +2.7 709,238 + ybfN b0682 0.69 +1.2 709,247 - ybfN b0682 0.72 -0.1 709,247 - ybfN b0682 0.72 -1.3 709,257 - ybfN b0682 0.75 +1.4 709,428 - +0.4 709,428 - +0.2 709,595 + fur b0683 0.38 -1.0 709,678 - fur b0683 0.57 -0.6 709,699 + fur b0683 0.62 -3.4 709,861 - -1.7
Or see this region's nucleotide sequence