Experiment: Tetrahymena sp., Mixed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nohB and ylcF overlap by 26 nucleotides ylcF and tfaD overlap by 3 nucleotides tfaD and ybcY are separated by 54 nucleotides ybcY and tfaX are separated by 146 nucleotides tfaX and appY are separated by 545 nucleotides
b0560: nohB - DLP12 prophage; DNA packaging protein (NCBI), at 580,057 to 580,602
nohB
b4634: ylcF - no description, at 580,577 to 580,885
ylcF
b0561: tfaD - orf, hypothetical protein (VIMSS), at 580,883 to 581,320
tfaD
b0562: ybcY - orf, hypothetical protein (VIMSS), at 581,375 to 582,029
ybcY
b0563: tfaX - DLP12 prophage; predicted protein (RefSeq), at 582,176 to 582,358
tfaX
b0564: appY - DLP12 prophage; DNA-binding transcriptional activator (NCBI), at 582,904 to 583,653
appY
Position (kb)
581
582
583 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 580.605 kb on - strand at 580.642 kb on + strand, within ylcF at 580.668 kb on - strand, within ylcF at 580.668 kb on - strand, within ylcF at 580.733 kb on + strand, within ylcF at 580.733 kb on + strand, within ylcF at 580.733 kb on + strand, within ylcF at 580.944 kb on - strand, within tfaD at 580.973 kb on + strand, within tfaD at 580.981 kb on - strand, within tfaD at 580.981 kb on - strand, within tfaD at 581.152 kb on + strand, within tfaD at 581.171 kb on + strand, within tfaD at 581.192 kb on - strand, within tfaD at 581.192 kb on - strand, within tfaD at 581.252 kb on + strand, within tfaD at 581.260 kb on - strand, within tfaD at 581.260 kb on - strand, within tfaD at 581.262 kb on + strand, within tfaD at 581.269 kb on - strand, within tfaD at 581.285 kb on + strand at 581.307 kb on + strand at 581.307 kb on + strand at 581.343 kb on + strand at 581.344 kb on + strand at 581.344 kb on + strand at 581.404 kb on + strand at 581.411 kb on + strand at 581.443 kb on + strand, within ybcY at 581.450 kb on + strand, within ybcY at 581.453 kb on - strand, within ybcY at 581.460 kb on + strand, within ybcY at 581.524 kb on + strand, within ybcY at 581.524 kb on + strand, within ybcY at 581.572 kb on + strand, within ybcY at 581.574 kb on + strand, within ybcY at 581.574 kb on + strand, within ybcY at 581.576 kb on + strand, within ybcY at 581.576 kb on + strand, within ybcY at 581.584 kb on - strand, within ybcY at 581.584 kb on - strand, within ybcY at 581.595 kb on + strand, within ybcY at 581.603 kb on - strand, within ybcY at 581.654 kb on + strand, within ybcY at 581.654 kb on + strand, within ybcY at 581.665 kb on - strand, within ybcY at 581.672 kb on - strand, within ybcY at 581.701 kb on - strand, within ybcY at 581.704 kb on - strand, within ybcY at 581.704 kb on - strand, within ybcY at 581.704 kb on - strand, within ybcY at 581.717 kb on - strand, within ybcY at 581.766 kb on + strand, within ybcY at 581.772 kb on + strand, within ybcY at 581.772 kb on - strand, within ybcY at 581.772 kb on - strand, within ybcY at 581.774 kb on - strand, within ybcY at 581.785 kb on + strand, within ybcY at 581.816 kb on - strand, within ybcY at 581.822 kb on + strand, within ybcY at 581.830 kb on - strand, within ybcY at 581.831 kb on + strand, within ybcY at 581.836 kb on + strand, within ybcY at 581.836 kb on + strand, within ybcY at 581.841 kb on + strand, within ybcY at 581.841 kb on + strand, within ybcY at 581.841 kb on - strand, within ybcY at 581.862 kb on - strand, within ybcY at 581.862 kb on - strand, within ybcY at 581.876 kb on + strand, within ybcY at 581.876 kb on + strand, within ybcY at 581.877 kb on - strand, within ybcY at 581.877 kb on - strand, within ybcY at 581.884 kb on - strand, within ybcY at 581.890 kb on - strand, within ybcY at 581.900 kb on + strand, within ybcY at 581.900 kb on + strand, within ybcY at 581.920 kb on + strand, within ybcY at 581.920 kb on + strand, within ybcY at 581.920 kb on - strand, within ybcY at 581.925 kb on - strand, within ybcY at 581.925 kb on - strand, within ybcY at 581.928 kb on - strand, within ybcY at 581.928 kb on - strand, within ybcY at 581.930 kb on + strand, within ybcY at 581.930 kb on + strand, within ybcY at 581.930 kb on + strand, within ybcY at 581.930 kb on + strand, within ybcY at 581.930 kb on + strand, within ybcY at 581.932 kb on + strand, within ybcY at 581.938 kb on - strand, within ybcY at 581.938 kb on - strand, within ybcY at 581.938 kb on - strand, within ybcY at 581.938 kb on - strand, within ybcY at 581.938 kb on - strand, within ybcY at 581.938 kb on - strand, within ybcY at 581.938 kb on - strand, within ybcY at 581.940 kb on + strand, within ybcY at 581.969 kb on + strand at 581.970 kb on + strand at 581.970 kb on + strand at 581.970 kb on + strand at 581.977 kb on + strand at 581.977 kb on + strand at 581.992 kb on - strand at 582.013 kb on + strand at 582.104 kb on + strand at 582.104 kb on + strand at 582.138 kb on + strand at 582.140 kb on - strand at 582.147 kb on + strand at 582.147 kb on + strand at 582.151 kb on - strand at 582.155 kb on - strand at 582.163 kb on + strand at 582.174 kb on + strand at 582.174 kb on + strand at 582.174 kb on + strand at 582.174 kb on + strand at 582.174 kb on + strand at 582.174 kb on + strand at 582.174 kb on + strand at 582.176 kb on + strand at 582.177 kb on + strand at 582.182 kb on - strand at 582.182 kb on - strand at 582.182 kb on - strand at 582.182 kb on - strand at 582.182 kb on - strand at 582.184 kb on + strand at 582.184 kb on + strand at 582.185 kb on - strand at 582.187 kb on - strand at 582.190 kb on - strand at 582.190 kb on - strand at 582.208 kb on + strand, within tfaX at 582.208 kb on + strand, within tfaX at 582.210 kb on + strand, within tfaX at 582.210 kb on + strand, within tfaX at 582.220 kb on + strand, within tfaX at 582.220 kb on + strand, within tfaX at 582.221 kb on + strand, within tfaX at 582.221 kb on + strand, within tfaX at 582.226 kb on + strand, within tfaX at 582.236 kb on + strand, within tfaX at 582.299 kb on + strand, within tfaX at 582.299 kb on + strand, within tfaX at 582.299 kb on + strand, within tfaX at 582.299 kb on + strand, within tfaX at 582.299 kb on + strand, within tfaX at 582.299 kb on + strand, within tfaX at 582.299 kb on + strand, within tfaX at 582.299 kb on + strand, within tfaX at 582.299 kb on + strand, within tfaX at 582.301 kb on + strand, within tfaX at 582.306 kb on + strand, within tfaX at 582.306 kb on + strand, within tfaX at 582.307 kb on + strand, within tfaX at 582.318 kb on + strand, within tfaX at 582.318 kb on + strand, within tfaX at 582.326 kb on + strand, within tfaX at 583.029 kb on + strand, within appY
Per-strain Table
Position Strand Gene LocusTag Fraction Tetrahymena sp., Mixed culture-1 remove 580,605 - -4.7 580,642 + ylcF b4634 0.21 +0.9 580,668 - ylcF b4634 0.29 -0.4 580,668 - ylcF b4634 0.29 +2.5 580,733 + ylcF b4634 0.50 -1.3 580,733 + ylcF b4634 0.50 -5.0 580,733 + ylcF b4634 0.50 +0.3 580,944 - tfaD b0561 0.14 -0.1 580,973 + tfaD b0561 0.21 -4.1 580,981 - tfaD b0561 0.22 +0.7 580,981 - tfaD b0561 0.22 +1.4 581,152 + tfaD b0561 0.61 +2.2 581,171 + tfaD b0561 0.66 +1.5 581,192 - tfaD b0561 0.71 -0.6 581,192 - tfaD b0561 0.71 -1.0 581,252 + tfaD b0561 0.84 +1.7 581,260 - tfaD b0561 0.86 -0.4 581,260 - tfaD b0561 0.86 +1.2 581,262 + tfaD b0561 0.87 -1.7 581,269 - tfaD b0561 0.88 -1.5 581,285 + -4.2 581,307 + +1.2 581,307 + +0.2 581,343 + -3.0 581,344 + +2.6 581,344 + -2.4 581,404 + -2.5 581,411 + +2.7 581,443 + ybcY b0562 0.10 +1.4 581,450 + ybcY b0562 0.11 +0.9 581,453 - ybcY b0562 0.12 +2.5 581,460 + ybcY b0562 0.13 -0.2 581,524 + ybcY b0562 0.23 -1.6 581,524 + ybcY b0562 0.23 -2.0 581,572 + ybcY b0562 0.30 -2.1 581,574 + ybcY b0562 0.30 -2.0 581,574 + ybcY b0562 0.30 -2.1 581,576 + ybcY b0562 0.31 -2.9 581,576 + ybcY b0562 0.31 +0.1 581,584 - ybcY b0562 0.32 -2.5 581,584 - ybcY b0562 0.32 -3.3 581,595 + ybcY b0562 0.34 +1.6 581,603 - ybcY b0562 0.35 +0.4 581,654 + ybcY b0562 0.43 -1.1 581,654 + ybcY b0562 0.43 +0.6 581,665 - ybcY b0562 0.44 +1.3 581,672 - ybcY b0562 0.45 -1.6 581,701 - ybcY b0562 0.50 -3.4 581,704 - ybcY b0562 0.50 -0.0 581,704 - ybcY b0562 0.50 +0.6 581,704 - ybcY b0562 0.50 -0.2 581,717 - ybcY b0562 0.52 -4.7 581,766 + ybcY b0562 0.60 +2.2 581,772 + ybcY b0562 0.61 +0.2 581,772 - ybcY b0562 0.61 -0.1 581,772 - ybcY b0562 0.61 -1.9 581,774 - ybcY b0562 0.61 -2.3 581,785 + ybcY b0562 0.63 -0.4 581,816 - ybcY b0562 0.67 -2.6 581,822 + ybcY b0562 0.68 -1.9 581,830 - ybcY b0562 0.69 -2.6 581,831 + ybcY b0562 0.70 -0.1 581,836 + ybcY b0562 0.70 -3.1 581,836 + ybcY b0562 0.70 +0.8 581,841 + ybcY b0562 0.71 -2.1 581,841 + ybcY b0562 0.71 +0.2 581,841 - ybcY b0562 0.71 -0.3 581,862 - ybcY b0562 0.74 -1.3 581,862 - ybcY b0562 0.74 +0.7 581,876 + ybcY b0562 0.76 -1.0 581,876 + ybcY b0562 0.76 +1.2 581,877 - ybcY b0562 0.77 -1.3 581,877 - ybcY b0562 0.77 -2.9 581,884 - ybcY b0562 0.78 +1.2 581,890 - ybcY b0562 0.79 +2.6 581,900 + ybcY b0562 0.80 -2.0 581,900 + ybcY b0562 0.80 +1.8 581,920 + ybcY b0562 0.83 -0.1 581,920 + ybcY b0562 0.83 -1.7 581,920 - ybcY b0562 0.83 -1.7 581,925 - ybcY b0562 0.84 +3.9 581,925 - ybcY b0562 0.84 -1.5 581,928 - ybcY b0562 0.84 -1.6 581,928 - ybcY b0562 0.84 -3.1 581,930 + ybcY b0562 0.85 +0.8 581,930 + ybcY b0562 0.85 -4.0 581,930 + ybcY b0562 0.85 -2.7 581,930 + ybcY b0562 0.85 -1.2 581,930 + ybcY b0562 0.85 -1.3 581,932 + ybcY b0562 0.85 -2.1 581,938 - ybcY b0562 0.86 -1.4 581,938 - ybcY b0562 0.86 +1.7 581,938 - ybcY b0562 0.86 +0.1 581,938 - ybcY b0562 0.86 -0.1 581,938 - ybcY b0562 0.86 +2.0 581,938 - ybcY b0562 0.86 +2.0 581,938 - ybcY b0562 0.86 -3.4 581,940 + ybcY b0562 0.86 -3.8 581,969 + -3.7 581,970 + -2.1 581,970 + +4.9 581,970 + -1.4 581,977 + -2.8 581,977 + -0.0 581,992 - -1.4 582,013 + +0.1 582,104 + +0.3 582,104 + -1.5 582,138 + -1.4 582,140 - +2.2 582,147 + +3.2 582,147 + -2.1 582,151 - +0.5 582,155 - +3.5 582,163 + +2.2 582,174 + +3.3 582,174 + -4.0 582,174 + -2.2 582,174 + +0.3 582,174 + +0.0 582,174 + +0.5 582,174 + +0.4 582,176 + -4.0 582,177 + +0.9 582,182 - +0.6 582,182 - +2.2 582,182 - +2.2 582,182 - -2.4 582,182 - +1.2 582,184 + -2.3 582,184 + -3.8 582,185 - +2.2 582,187 - +1.2 582,190 - +1.2 582,190 - +2.2 582,208 + tfaX b0563 0.17 -2.3 582,208 + tfaX b0563 0.17 -2.6 582,210 + tfaX b0563 0.19 +3.9 582,210 + tfaX b0563 0.19 -4.1 582,220 + tfaX b0563 0.24 +1.9 582,220 + tfaX b0563 0.24 -0.9 582,221 + tfaX b0563 0.25 -3.4 582,221 + tfaX b0563 0.25 +2.7 582,226 + tfaX b0563 0.27 +0.8 582,236 + tfaX b0563 0.33 +2.7 582,299 + tfaX b0563 0.67 +3.1 582,299 + tfaX b0563 0.67 -1.7 582,299 + tfaX b0563 0.67 -2.1 582,299 + tfaX b0563 0.67 -0.5 582,299 + tfaX b0563 0.67 +0.9 582,299 + tfaX b0563 0.67 +0.7 582,299 + tfaX b0563 0.67 -3.3 582,299 + tfaX b0563 0.67 -1.5 582,299 + tfaX b0563 0.67 +2.5 582,301 + tfaX b0563 0.68 -1.9 582,306 + tfaX b0563 0.71 -2.3 582,306 + tfaX b0563 0.71 +0.1 582,307 + tfaX b0563 0.72 -2.4 582,318 + tfaX b0563 0.78 -2.9 582,318 + tfaX b0563 0.78 +1.2 582,326 + tfaX b0563 0.82 +4.0 583,029 + appY b0564 0.17 +2.2
Or see this region's nucleotide sequence