Experiment: Tetrahymena sp., Mixed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt apaH and apaG are separated by 6 nucleotides apaG and ksgA are separated by 2 nucleotides ksgA and pdxA overlap by 4 nucleotides pdxA and surA overlap by 1 nucleotides
b0049: apaH - diadenosinetetraphosphatase (NCBI), at 50,380 to 51,222
apaH
b0050: apaG - hypothetical protein (NCBI), at 51,229 to 51,606
apaG
b0051: ksgA - dimethyladenosine transferase (NCBI), at 51,609 to 52,430
ksgA
b0052: pdxA - 4-hydroxythreonine-4-phosphate dehydrogenase (NCBI), at 52,427 to 53,416
pdxA
b0053: surA - peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI), at 53,416 to 54,702
surA
Position (kb)
51
52
53 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 50.717 kb on - strand, within apaH at 50.785 kb on - strand, within apaH at 50.791 kb on + strand, within apaH at 50.802 kb on + strand, within apaH at 50.829 kb on - strand, within apaH at 50.859 kb on - strand, within apaH at 50.877 kb on + strand, within apaH at 51.025 kb on + strand, within apaH at 51.109 kb on - strand, within apaH at 51.177 kb on + strand at 51.177 kb on + strand at 51.185 kb on - strand at 51.190 kb on + strand at 51.190 kb on + strand at 51.240 kb on + strand at 51.240 kb on + strand at 51.311 kb on + strand, within apaG at 51.311 kb on + strand, within apaG at 51.322 kb on - strand, within apaG at 51.326 kb on - strand, within apaG at 51.334 kb on - strand, within apaG at 51.336 kb on - strand, within apaG at 51.353 kb on + strand, within apaG at 51.353 kb on + strand, within apaG at 51.353 kb on - strand, within apaG at 51.364 kb on - strand, within apaG at 51.372 kb on - strand, within apaG at 51.415 kb on + strand, within apaG at 51.423 kb on - strand, within apaG at 51.466 kb on + strand, within apaG at 51.472 kb on + strand, within apaG at 51.512 kb on - strand, within apaG at 51.514 kb on - strand, within apaG at 51.520 kb on - strand, within apaG at 51.524 kb on + strand, within apaG at 51.550 kb on - strand, within apaG at 51.560 kb on + strand, within apaG at 51.567 kb on - strand, within apaG at 51.567 kb on - strand, within apaG at 51.576 kb on + strand at 51.576 kb on + strand at 51.586 kb on - strand at 51.613 kb on + strand at 51.614 kb on - strand at 51.615 kb on + strand at 51.615 kb on + strand at 51.673 kb on - strand at 51.703 kb on + strand, within ksgA at 51.703 kb on + strand, within ksgA at 51.812 kb on - strand, within ksgA at 51.836 kb on + strand, within ksgA at 51.891 kb on - strand, within ksgA at 51.891 kb on - strand, within ksgA at 51.904 kb on + strand, within ksgA at 51.906 kb on + strand, within ksgA at 51.925 kb on - strand, within ksgA at 52.040 kb on + strand, within ksgA at 52.040 kb on + strand, within ksgA at 52.040 kb on + strand, within ksgA at 52.042 kb on + strand, within ksgA at 52.042 kb on + strand, within ksgA at 52.042 kb on + strand, within ksgA at 52.042 kb on - strand, within ksgA at 52.042 kb on - strand, within ksgA at 52.045 kb on + strand, within ksgA at 52.062 kb on + strand, within ksgA at 52.062 kb on + strand at 52.070 kb on - strand, within ksgA at 52.072 kb on + strand, within ksgA at 52.089 kb on - strand, within ksgA at 52.276 kb on + strand, within ksgA at 52.276 kb on + strand, within ksgA at 52.284 kb on + strand, within ksgA at 52.338 kb on + strand, within ksgA at 52.338 kb on + strand, within ksgA at 52.343 kb on - strand, within ksgA at 52.348 kb on - strand at 52.348 kb on - strand at 52.348 kb on - strand at 52.354 kb on + strand at 52.420 kb on - strand at 52.490 kb on + strand at 52.490 kb on + strand at 52.502 kb on + strand at 52.502 kb on + strand at 52.518 kb on - strand at 52.518 kb on - strand at 52.626 kb on + strand, within pdxA at 52.688 kb on - strand, within pdxA at 52.735 kb on + strand, within pdxA at 52.735 kb on + strand, within pdxA at 52.740 kb on - strand, within pdxA at 52.766 kb on - strand, within pdxA at 52.780 kb on - strand, within pdxA at 52.797 kb on - strand, within pdxA at 52.879 kb on - strand, within pdxA at 52.914 kb on + strand, within pdxA at 52.982 kb on - strand, within pdxA at 52.982 kb on - strand, within pdxA at 52.983 kb on + strand, within pdxA at 53.061 kb on - strand, within pdxA at 53.062 kb on - strand, within pdxA at 53.062 kb on - strand at 53.064 kb on + strand, within pdxA at 53.067 kb on - strand, within pdxA at 53.163 kb on - strand, within pdxA at 53.163 kb on - strand, within pdxA at 53.181 kb on - strand, within pdxA at 53.181 kb on - strand, within pdxA at 53.202 kb on - strand, within pdxA at 53.221 kb on + strand, within pdxA at 53.221 kb on + strand, within pdxA at 53.226 kb on - strand, within pdxA at 53.246 kb on - strand, within pdxA at 53.291 kb on + strand, within pdxA at 53.328 kb on + strand at 53.328 kb on + strand at 53.338 kb on + strand at 53.338 kb on + strand at 53.338 kb on + strand at 53.338 kb on + strand at 53.346 kb on - strand at 53.346 kb on - strand at 53.346 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetrahymena sp., Mixed culture-1 remove 50,717 - apaH b0049 0.40 -1.9 50,785 - apaH b0049 0.48 -2.1 50,791 + apaH b0049 0.49 -0.7 50,802 + apaH b0049 0.50 -1.7 50,829 - apaH b0049 0.53 +1.1 50,859 - apaH b0049 0.57 -1.4 50,877 + apaH b0049 0.59 +0.1 51,025 + apaH b0049 0.77 -1.7 51,109 - apaH b0049 0.86 -2.8 51,177 + -0.7 51,177 + -1.2 51,185 - -2.0 51,190 + -2.5 51,190 + +0.9 51,240 + -0.3 51,240 + +0.1 51,311 + apaG b0050 0.22 -2.9 51,311 + apaG b0050 0.22 -2.8 51,322 - apaG b0050 0.25 -1.6 51,326 - apaG b0050 0.26 -2.4 51,334 - apaG b0050 0.28 +0.1 51,336 - apaG b0050 0.28 -0.4 51,353 + apaG b0050 0.33 -0.0 51,353 + apaG b0050 0.33 +1.2 51,353 - apaG b0050 0.33 +0.1 51,364 - apaG b0050 0.36 -0.3 51,372 - apaG b0050 0.38 -0.1 51,415 + apaG b0050 0.49 +1.8 51,423 - apaG b0050 0.51 -0.1 51,466 + apaG b0050 0.63 +3.0 51,472 + apaG b0050 0.64 -2.1 51,512 - apaG b0050 0.75 -3.9 51,514 - apaG b0050 0.75 -0.8 51,520 - apaG b0050 0.77 +1.6 51,524 + apaG b0050 0.78 -3.3 51,550 - apaG b0050 0.85 -1.9 51,560 + apaG b0050 0.88 -1.0 51,567 - apaG b0050 0.89 +0.1 51,567 - apaG b0050 0.89 -1.8 51,576 + -2.0 51,576 + -3.4 51,586 - +0.1 51,613 + -3.2 51,614 - -0.7 51,615 + -2.2 51,615 + +0.6 51,673 - -2.2 51,703 + ksgA b0051 0.11 -0.7 51,703 + ksgA b0051 0.11 -1.8 51,812 - ksgA b0051 0.25 -2.1 51,836 + ksgA b0051 0.28 -1.6 51,891 - ksgA b0051 0.34 -1.8 51,891 - ksgA b0051 0.34 -1.7 51,904 + ksgA b0051 0.36 -2.0 51,906 + ksgA b0051 0.36 -0.9 51,925 - ksgA b0051 0.38 -1.7 52,040 + ksgA b0051 0.52 -2.5 52,040 + ksgA b0051 0.52 -1.4 52,040 + ksgA b0051 0.52 -1.1 52,042 + ksgA b0051 0.53 -3.1 52,042 + ksgA b0051 0.53 -1.4 52,042 + ksgA b0051 0.53 -1.9 52,042 - ksgA b0051 0.53 -0.5 52,042 - ksgA b0051 0.53 -1.7 52,045 + ksgA b0051 0.53 -2.2 52,062 + ksgA b0051 0.55 -2.2 52,062 + +0.1 52,070 - ksgA b0051 0.56 -0.9 52,072 + ksgA b0051 0.56 -0.5 52,089 - ksgA b0051 0.58 -0.5 52,276 + ksgA b0051 0.81 -0.2 52,276 + ksgA b0051 0.81 -2.9 52,284 + ksgA b0051 0.82 -2.7 52,338 + ksgA b0051 0.89 -0.2 52,338 + ksgA b0051 0.89 -1.4 52,343 - ksgA b0051 0.89 -2.3 52,348 - -1.1 52,348 - +1.7 52,348 - -3.0 52,354 + -2.3 52,420 - +1.4 52,490 + -1.8 52,490 + -1.5 52,502 + -2.8 52,502 + -2.8 52,518 - -0.4 52,518 - -3.4 52,626 + pdxA b0052 0.20 -0.8 52,688 - pdxA b0052 0.26 -0.4 52,735 + pdxA b0052 0.31 -3.1 52,735 + pdxA b0052 0.31 -2.4 52,740 - pdxA b0052 0.32 +0.5 52,766 - pdxA b0052 0.34 -0.7 52,780 - pdxA b0052 0.36 -3.6 52,797 - pdxA b0052 0.37 -1.1 52,879 - pdxA b0052 0.46 +0.4 52,914 + pdxA b0052 0.49 -2.9 52,982 - pdxA b0052 0.56 -3.2 52,982 - pdxA b0052 0.56 +0.4 52,983 + pdxA b0052 0.56 -1.2 53,061 - pdxA b0052 0.64 +0.2 53,062 - pdxA b0052 0.64 -0.1 53,062 - +2.1 53,064 + pdxA b0052 0.64 +1.2 53,067 - pdxA b0052 0.65 -3.0 53,163 - pdxA b0052 0.74 -0.1 53,163 - pdxA b0052 0.74 +0.4 53,181 - pdxA b0052 0.76 +2.1 53,181 - pdxA b0052 0.76 +0.4 53,202 - pdxA b0052 0.78 -3.4 53,221 + pdxA b0052 0.80 -1.6 53,221 + pdxA b0052 0.80 +0.1 53,226 - pdxA b0052 0.81 -1.5 53,246 - pdxA b0052 0.83 -0.5 53,291 + pdxA b0052 0.87 -3.4 53,328 + -4.7 53,328 + +0.1 53,338 + -3.8 53,338 + -1.8 53,338 + -1.0 53,338 + +0.6 53,346 - -1.8 53,346 - +0.0 53,346 - -0.6
Or see this region's nucleotide sequence