Experiment: NLDM_defined; midlog
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS18665 and LRK53_RS18670 are separated by 9 nucleotides LRK53_RS18670 and LRK53_RS18675 overlap by 41 nucleotides LRK53_RS18675 and LRK53_RS18680 are separated by 16 nucleotides LRK53_RS18680 and LRK53_RS18685 are separated by 188 nucleotides
LRK53_RS18665: LRK53_RS18665 - hypothetical protein, at 119,475 to 120,146
_RS18665
LRK53_RS18670: LRK53_RS18670 - translesion DNA synthesis-associated protein ImuA, at 120,156 to 120,818
_RS18670
LRK53_RS18675: LRK53_RS18675 - DNA polymerase Y family protein, at 120,778 to 122,163
_RS18675
LRK53_RS18680: LRK53_RS18680 - hypothetical protein, at 122,180 to 122,911
_RS18680
LRK53_RS18685: LRK53_RS18685 - error-prone DNA polymerase, at 123,100 to 126,165
_RS18685
Position (kb)
120
121
122
123 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 119.923 kb on - strand, within LRK53_RS18665 at 119.923 kb on - strand, within LRK53_RS18665 at 119.923 kb on - strand, within LRK53_RS18665 at 119.923 kb on - strand, within LRK53_RS18665 at 119.961 kb on + strand, within LRK53_RS18665 at 119.961 kb on + strand, within LRK53_RS18665 at 120.314 kb on - strand, within LRK53_RS18670 at 120.314 kb on - strand, within LRK53_RS18670 at 120.316 kb on + strand, within LRK53_RS18670 at 120.316 kb on + strand, within LRK53_RS18670 at 120.316 kb on + strand, within LRK53_RS18670 at 120.316 kb on + strand, within LRK53_RS18670 at 120.317 kb on - strand, within LRK53_RS18670 at 120.317 kb on - strand, within LRK53_RS18670 at 120.317 kb on - strand, within LRK53_RS18670 at 120.727 kb on + strand, within LRK53_RS18670 at 120.727 kb on + strand, within LRK53_RS18670 at 120.727 kb on + strand, within LRK53_RS18670 at 120.727 kb on + strand, within LRK53_RS18670 at 120.728 kb on - strand, within LRK53_RS18670 at 120.728 kb on - strand, within LRK53_RS18670 at 120.728 kb on - strand, within LRK53_RS18670 at 121.469 kb on + strand, within LRK53_RS18675 at 121.470 kb on - strand, within LRK53_RS18675 at 121.555 kb on + strand, within LRK53_RS18675 at 121.556 kb on - strand, within LRK53_RS18675 at 122.071 kb on + strand at 122.113 kb on + strand at 122.113 kb on + strand at 122.114 kb on - strand at 122.114 kb on - strand at 122.233 kb on - strand at 122.481 kb on + strand, within LRK53_RS18680 at 122.481 kb on + strand, within LRK53_RS18680 at 122.481 kb on + strand, within LRK53_RS18680 at 122.481 kb on + strand, within LRK53_RS18680 at 122.481 kb on + strand, within LRK53_RS18680 at 122.481 kb on + strand, within LRK53_RS18680 at 122.481 kb on + strand, within LRK53_RS18680 at 122.481 kb on + strand, within LRK53_RS18680 at 122.481 kb on + strand, within LRK53_RS18680 at 122.481 kb on + strand, within LRK53_RS18680 at 122.482 kb on - strand, within LRK53_RS18680 at 122.482 kb on - strand, within LRK53_RS18680 at 122.482 kb on - strand, within LRK53_RS18680 at 122.482 kb on - strand, within LRK53_RS18680 at 122.482 kb on - strand, within LRK53_RS18680 at 122.482 kb on - strand, within LRK53_RS18680 at 122.482 kb on - strand, within LRK53_RS18680 at 122.482 kb on - strand, within LRK53_RS18680 at 122.482 kb on - strand, within LRK53_RS18680 at 122.482 kb on - strand, within LRK53_RS18680 at 122.482 kb on - strand, within LRK53_RS18680 at 122.787 kb on + strand, within LRK53_RS18680 at 122.787 kb on + strand, within LRK53_RS18680 at 122.788 kb on - strand, within LRK53_RS18680 at 122.788 kb on - strand, within LRK53_RS18680 at 122.788 kb on - strand, within LRK53_RS18680 at 122.788 kb on - strand, within LRK53_RS18680 at 122.788 kb on - strand, within LRK53_RS18680 at 122.788 kb on - strand, within LRK53_RS18680 at 122.857 kb on + strand at 122.857 kb on + strand at 122.857 kb on + strand at 122.857 kb on + strand at 122.857 kb on + strand at 122.857 kb on + strand at 122.858 kb on - strand at 122.858 kb on - strand at 122.858 kb on - strand at 122.858 kb on - strand at 122.858 kb on - strand at 122.858 kb on - strand at 122.858 kb on - strand at 122.902 kb on + strand at 122.902 kb on + strand at 122.903 kb on - strand at 122.986 kb on + strand at 122.986 kb on + strand at 122.986 kb on + strand at 122.986 kb on + strand at 122.986 kb on + strand at 122.986 kb on + strand at 122.987 kb on - strand at 122.987 kb on - strand at 122.987 kb on - strand at 123.060 kb on + strand at 123.060 kb on + strand at 123.060 kb on + strand at 123.103 kb on + strand at 123.103 kb on + strand at 123.103 kb on + strand at 123.103 kb on + strand at 123.103 kb on + strand at 123.103 kb on + strand at 123.103 kb on + strand at 123.103 kb on + strand at 123.103 kb on + strand at 123.104 kb on - strand at 123.104 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction NLDM_defined; midlog remove 119,923 - LRK53_RS18665 0.67 +1.0 119,923 - LRK53_RS18665 0.67 +0.3 119,923 - LRK53_RS18665 0.67 -0.0 119,923 - LRK53_RS18665 0.67 +0.6 119,961 + LRK53_RS18665 0.72 +1.5 119,961 + LRK53_RS18665 0.72 +1.3 120,314 - LRK53_RS18670 0.24 +0.1 120,314 - LRK53_RS18670 0.24 +0.3 120,316 + LRK53_RS18670 0.24 +2.6 120,316 + LRK53_RS18670 0.24 -1.9 120,316 + LRK53_RS18670 0.24 +2.3 120,316 + LRK53_RS18670 0.24 +0.7 120,317 - LRK53_RS18670 0.24 +2.0 120,317 - LRK53_RS18670 0.24 -0.7 120,317 - LRK53_RS18670 0.24 +0.3 120,727 + LRK53_RS18670 0.86 -1.2 120,727 + LRK53_RS18670 0.86 -2.0 120,727 + LRK53_RS18670 0.86 -0.3 120,727 + LRK53_RS18670 0.86 +0.3 120,728 - LRK53_RS18670 0.86 -0.0 120,728 - LRK53_RS18670 0.86 +1.3 120,728 - LRK53_RS18670 0.86 -0.5 121,469 + LRK53_RS18675 0.50 -0.6 121,470 - LRK53_RS18675 0.50 -0.3 121,555 + LRK53_RS18675 0.56 -1.0 121,556 - LRK53_RS18675 0.56 -0.5 122,071 + -0.9 122,113 + -0.7 122,113 + +0.3 122,114 - -0.8 122,114 - -1.3 122,233 - -2.3 122,481 + LRK53_RS18680 0.41 +1.3 122,481 + LRK53_RS18680 0.41 -1.5 122,481 + LRK53_RS18680 0.41 +1.3 122,481 + LRK53_RS18680 0.41 -1.7 122,481 + LRK53_RS18680 0.41 -1.0 122,481 + LRK53_RS18680 0.41 +1.3 122,481 + LRK53_RS18680 0.41 -1.3 122,481 + LRK53_RS18680 0.41 -0.3 122,481 + LRK53_RS18680 0.41 -0.7 122,481 + LRK53_RS18680 0.41 -1.1 122,482 - LRK53_RS18680 0.41 -1.7 122,482 - LRK53_RS18680 0.41 -0.2 122,482 - LRK53_RS18680 0.41 +0.8 122,482 - LRK53_RS18680 0.41 +0.5 122,482 - LRK53_RS18680 0.41 +2.0 122,482 - LRK53_RS18680 0.41 -1.5 122,482 - LRK53_RS18680 0.41 +1.8 122,482 - LRK53_RS18680 0.41 -0.5 122,482 - LRK53_RS18680 0.41 -2.8 122,482 - LRK53_RS18680 0.41 -0.5 122,482 - LRK53_RS18680 0.41 +0.1 122,787 + LRK53_RS18680 0.83 +2.3 122,787 + LRK53_RS18680 0.83 +1.7 122,788 - LRK53_RS18680 0.83 -0.8 122,788 - LRK53_RS18680 0.83 -1.8 122,788 - LRK53_RS18680 0.83 +0.3 122,788 - LRK53_RS18680 0.83 +2.1 122,788 - LRK53_RS18680 0.83 -0.7 122,788 - LRK53_RS18680 0.83 -1.0 122,857 + +0.3 122,857 + -0.9 122,857 + -1.3 122,857 + -1.0 122,857 + +2.4 122,857 + -0.3 122,858 - -0.3 122,858 - +0.8 122,858 - -1.3 122,858 - +2.0 122,858 - -1.7 122,858 - -0.6 122,858 - -0.5 122,902 + +0.7 122,902 + -1.0 122,903 - +0.1 122,986 + +0.3 122,986 + -0.3 122,986 + +0.7 122,986 + +0.9 122,986 + -0.4 122,986 + +2.9 122,987 - +0.5 122,987 - +0.8 122,987 - -0.9 123,060 + -0.0 123,060 + +2.9 123,060 + +0.3 123,103 + +0.1 123,103 + +0.1 123,103 + -0.0 123,103 + -0.2 123,103 + +2.3 123,103 + -0.4 123,103 + -0.1 123,103 + -1.0 123,103 + +0.4 123,104 - -1.5 123,104 - -0.2
Or see this region's nucleotide sequence