Experiment: NLDM_defined; midlog
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS18285 and LRK53_RS18290 are separated by 151 nucleotides LRK53_RS18290 and LRK53_RS18295 are separated by 250 nucleotides LRK53_RS18295 and LRK53_RS18300 are separated by 86 nucleotides LRK53_RS18300 and LRK53_RS18305 are separated by 2 nucleotides
LRK53_RS18285: LRK53_RS18285 - hypothetical protein, at 34,711 to 34,926
_RS18285
LRK53_RS18290: LRK53_RS18290 - hypothetical protein, at 35,078 to 35,251
_RS18290
LRK53_RS18295: LRK53_RS18295 - hypothetical protein, at 35,502 to 36,113
_RS18295
LRK53_RS18300: LRK53_RS18300 - hypothetical protein, at 36,200 to 37,108
_RS18300
LRK53_RS18305: LRK53_RS18305 - PD-(D/E)XK nuclease family protein, at 37,111 to 38,037
_RS18305
Position (kb) 35
36
37 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 34.618 kb on + strand at 34.619 kb on - strand at 34.619 kb on - strand at 34.772 kb on - strand, within LRK53_RS18285 at 34.772 kb on - strand, within LRK53_RS18285 at 34.772 kb on - strand, within LRK53_RS18285 at 34.772 kb on - strand, within LRK53_RS18285 at 34.772 kb on - strand, within LRK53_RS18285 at 34.816 kb on - strand, within LRK53_RS18285 at 34.840 kb on + strand, within LRK53_RS18285 at 34.864 kb on - strand, within LRK53_RS18285 at 35.016 kb on - strand at 35.071 kb on + strand at 35.071 kb on + strand at 35.071 kb on + strand at 35.071 kb on + strand at 35.072 kb on - strand at 35.072 kb on - strand at 35.072 kb on - strand at 35.072 kb on - strand at 35.072 kb on - strand at 35.233 kb on + strand, within LRK53_RS18290 at 35.234 kb on - strand at 35.438 kb on + strand at 35.438 kb on + strand at 35.637 kb on + strand, within LRK53_RS18295 at 35.715 kb on + strand, within LRK53_RS18295 at 35.716 kb on - strand, within LRK53_RS18295 at 35.716 kb on - strand, within LRK53_RS18295 at 35.716 kb on - strand, within LRK53_RS18295 at 36.065 kb on + strand at 36.066 kb on - strand at 36.066 kb on - strand at 36.066 kb on - strand at 36.066 kb on - strand at 36.126 kb on + strand at 36.144 kb on + strand at 36.145 kb on - strand at 36.264 kb on - strand at 36.264 kb on - strand at 36.338 kb on + strand, within LRK53_RS18300 at 36.338 kb on + strand, within LRK53_RS18300 at 36.338 kb on + strand, within LRK53_RS18300 at 36.338 kb on + strand, within LRK53_RS18300 at 36.533 kb on + strand, within LRK53_RS18300 at 36.599 kb on + strand, within LRK53_RS18300 at 36.599 kb on + strand, within LRK53_RS18300 at 36.599 kb on + strand, within LRK53_RS18300 at 36.599 kb on + strand, within LRK53_RS18300 at 36.599 kb on + strand, within LRK53_RS18300 at 36.600 kb on - strand, within LRK53_RS18300 at 37.000 kb on + strand, within LRK53_RS18300 at 37.000 kb on + strand, within LRK53_RS18300 at 37.059 kb on - strand at 37.059 kb on - strand at 37.059 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction NLDM_defined; midlog remove 34,618 + -0.1 34,619 - -0.3 34,619 - +2.9 34,772 - LRK53_RS18285 0.28 -0.3 34,772 - LRK53_RS18285 0.28 +0.3 34,772 - LRK53_RS18285 0.28 +0.3 34,772 - LRK53_RS18285 0.28 -0.5 34,772 - LRK53_RS18285 0.28 +0.0 34,816 - LRK53_RS18285 0.49 +0.5 34,840 + LRK53_RS18285 0.60 -1.7 34,864 - LRK53_RS18285 0.71 -0.0 35,016 - -0.5 35,071 + -0.9 35,071 + +0.7 35,071 + -1.0 35,071 + +0.5 35,072 - -0.3 35,072 - +0.6 35,072 - -0.6 35,072 - -0.1 35,072 - +1.3 35,233 + LRK53_RS18290 0.89 -1.5 35,234 - -0.4 35,438 + -0.5 35,438 + +1.3 35,637 + LRK53_RS18295 0.22 -1.7 35,715 + LRK53_RS18295 0.35 -1.1 35,716 - LRK53_RS18295 0.35 -1.0 35,716 - LRK53_RS18295 0.35 +0.3 35,716 - LRK53_RS18295 0.35 -0.9 36,065 + +0.0 36,066 - -1.0 36,066 - -1.6 36,066 - -0.5 36,066 - -1.3 36,126 + -0.1 36,144 + +2.3 36,145 - -2.0 36,264 - +1.0 36,264 - +0.3 36,338 + LRK53_RS18300 0.15 -0.9 36,338 + LRK53_RS18300 0.15 -1.0 36,338 + LRK53_RS18300 0.15 +0.8 36,338 + LRK53_RS18300 0.15 -0.8 36,533 + LRK53_RS18300 0.37 -0.4 36,599 + LRK53_RS18300 0.44 +0.3 36,599 + LRK53_RS18300 0.44 -0.7 36,599 + LRK53_RS18300 0.44 -1.0 36,599 + LRK53_RS18300 0.44 -2.0 36,599 + LRK53_RS18300 0.44 -1.7 36,600 - LRK53_RS18300 0.44 -0.2 37,000 + LRK53_RS18300 0.88 +1.6 37,000 + LRK53_RS18300 0.88 -0.9 37,059 - +0.3 37,059 - -1.0 37,059 - +0.8
Or see this region's nucleotide sequence