Experiment: NLDM_defined; midlog
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS05160 and LRK53_RS05165 overlap by 1 nucleotides LRK53_RS05165 and LRK53_RS05170 are separated by 12 nucleotides LRK53_RS05170 and LRK53_RS05175 are separated by 151 nucleotides LRK53_RS05175 and LRK53_RS05180 are separated by 72 nucleotides
LRK53_RS05160: LRK53_RS05160 - peptidoglycan-associated lipoprotein Pal, at 1,212,835 to 1,213,341
_RS05160
LRK53_RS05165: LRK53_RS05165 - tol-pal system protein YbgF, at 1,213,341 to 1,214,210
_RS05165
LRK53_RS05170: LRK53_RS05170 - 7-carboxy-7-deazaguanine synthase QueE, at 1,214,223 to 1,214,915
_RS05170
LRK53_RS05175: LRK53_RS05175 - 7-cyano-7-deazaguanine synthase QueC, at 1,215,067 to 1,215,753
_RS05175
LRK53_RS05180: LRK53_RS05180 - tRNA-Lys, at 1,215,826 to 1,215,901
_RS05180
Position (kb)
1214
1215 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1213.623 kb on + strand, within LRK53_RS05165 at 1213.623 kb on + strand, within LRK53_RS05165 at 1213.623 kb on + strand, within LRK53_RS05165 at 1213.623 kb on + strand, within LRK53_RS05165 at 1213.624 kb on - strand, within LRK53_RS05165 at 1213.624 kb on - strand, within LRK53_RS05165 at 1213.624 kb on - strand, within LRK53_RS05165 at 1213.624 kb on - strand, within LRK53_RS05165 at 1213.624 kb on - strand, within LRK53_RS05165 at 1213.624 kb on - strand, within LRK53_RS05165 at 1213.839 kb on + strand, within LRK53_RS05165 at 1213.839 kb on + strand, within LRK53_RS05165 at 1213.839 kb on + strand, within LRK53_RS05165 at 1213.839 kb on + strand, within LRK53_RS05165 at 1213.840 kb on - strand, within LRK53_RS05165 at 1213.840 kb on - strand, within LRK53_RS05165 at 1213.875 kb on + strand, within LRK53_RS05165 at 1213.875 kb on + strand, within LRK53_RS05165 at 1213.875 kb on + strand, within LRK53_RS05165 at 1213.875 kb on + strand, within LRK53_RS05165 at 1213.875 kb on + strand, within LRK53_RS05165 at 1213.875 kb on + strand, within LRK53_RS05165 at 1213.876 kb on - strand, within LRK53_RS05165 at 1213.876 kb on - strand, within LRK53_RS05165 at 1213.876 kb on - strand, within LRK53_RS05165 at 1213.950 kb on + strand, within LRK53_RS05165 at 1213.950 kb on + strand, within LRK53_RS05165 at 1213.968 kb on + strand, within LRK53_RS05165 at 1213.968 kb on + strand, within LRK53_RS05165 at 1213.969 kb on - strand, within LRK53_RS05165 at 1213.969 kb on - strand, within LRK53_RS05165 at 1213.969 kb on - strand, within LRK53_RS05165 at 1213.971 kb on + strand, within LRK53_RS05165 at 1213.971 kb on + strand, within LRK53_RS05165 at 1213.971 kb on + strand, within LRK53_RS05165 at 1213.971 kb on + strand, within LRK53_RS05165 at 1213.971 kb on + strand, within LRK53_RS05165 at 1213.971 kb on + strand, within LRK53_RS05165 at 1213.972 kb on - strand, within LRK53_RS05165 at 1213.972 kb on - strand, within LRK53_RS05165 at 1213.972 kb on - strand, within LRK53_RS05165 at 1213.972 kb on - strand, within LRK53_RS05165 at 1213.972 kb on - strand, within LRK53_RS05165 at 1213.986 kb on + strand, within LRK53_RS05165 at 1213.987 kb on - strand, within LRK53_RS05165 at 1213.987 kb on - strand, within LRK53_RS05165 at 1213.987 kb on - strand, within LRK53_RS05165 at 1214.074 kb on - strand, within LRK53_RS05165 at 1214.074 kb on - strand, within LRK53_RS05165 at 1214.140 kb on - strand at 1214.222 kb on + strand at 1214.222 kb on + strand at 1214.222 kb on + strand at 1214.222 kb on + strand at 1214.223 kb on - strand at 1214.223 kb on - strand at 1214.223 kb on - strand at 1214.223 kb on - strand at 1214.223 kb on - strand at 1214.400 kb on + strand, within LRK53_RS05170 at 1214.400 kb on + strand, within LRK53_RS05170 at 1214.400 kb on + strand, within LRK53_RS05170 at 1214.401 kb on - strand, within LRK53_RS05170 at 1214.401 kb on - strand, within LRK53_RS05170 at 1214.401 kb on - strand, within LRK53_RS05170 at 1214.731 kb on - strand, within LRK53_RS05170 at 1214.731 kb on - strand, within LRK53_RS05170 at 1214.762 kb on + strand, within LRK53_RS05170 at 1214.913 kb on + strand at 1214.914 kb on - strand at 1214.914 kb on - strand at 1214.914 kb on - strand at 1214.995 kb on + strand at 1214.996 kb on - strand at 1214.996 kb on - strand at 1215.185 kb on - strand, within LRK53_RS05175 at 1215.358 kb on + strand, within LRK53_RS05175 at 1215.358 kb on + strand, within LRK53_RS05175 at 1215.358 kb on + strand, within LRK53_RS05175 at 1215.359 kb on - strand, within LRK53_RS05175 at 1215.359 kb on - strand, within LRK53_RS05175 at 1215.359 kb on - strand, within LRK53_RS05175 at 1215.647 kb on - strand, within LRK53_RS05175 at 1215.746 kb on - strand at 1215.776 kb on + strand at 1215.776 kb on + strand at 1215.776 kb on + strand at 1215.776 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction NLDM_defined; midlog remove 1,213,623 + LRK53_RS05165 0.32 +0.3 1,213,623 + LRK53_RS05165 0.32 -0.8 1,213,623 + LRK53_RS05165 0.32 +0.5 1,213,623 + LRK53_RS05165 0.32 +0.3 1,213,624 - LRK53_RS05165 0.33 -0.3 1,213,624 - LRK53_RS05165 0.33 -0.7 1,213,624 - LRK53_RS05165 0.33 +0.3 1,213,624 - LRK53_RS05165 0.33 -2.8 1,213,624 - LRK53_RS05165 0.33 -3.2 1,213,624 - LRK53_RS05165 0.33 +1.3 1,213,839 + LRK53_RS05165 0.57 +1.3 1,213,839 + LRK53_RS05165 0.57 -0.1 1,213,839 + LRK53_RS05165 0.57 -0.7 1,213,839 + LRK53_RS05165 0.57 -0.7 1,213,840 - LRK53_RS05165 0.57 -3.0 1,213,840 - LRK53_RS05165 0.57 -1.8 1,213,875 + LRK53_RS05165 0.61 +0.7 1,213,875 + LRK53_RS05165 0.61 -1.3 1,213,875 + LRK53_RS05165 0.61 -2.8 1,213,875 + LRK53_RS05165 0.61 -0.5 1,213,875 + LRK53_RS05165 0.61 +0.9 1,213,875 + LRK53_RS05165 0.61 -0.0 1,213,876 - LRK53_RS05165 0.61 +0.3 1,213,876 - LRK53_RS05165 0.61 +0.7 1,213,876 - LRK53_RS05165 0.61 -3.6 1,213,950 + LRK53_RS05165 0.70 +0.0 1,213,950 + LRK53_RS05165 0.70 +0.6 1,213,968 + LRK53_RS05165 0.72 -2.0 1,213,968 + LRK53_RS05165 0.72 -0.7 1,213,969 - LRK53_RS05165 0.72 -0.3 1,213,969 - LRK53_RS05165 0.72 -0.6 1,213,969 - LRK53_RS05165 0.72 +1.8 1,213,971 + LRK53_RS05165 0.72 -0.6 1,213,971 + LRK53_RS05165 0.72 -0.1 1,213,971 + LRK53_RS05165 0.72 +1.3 1,213,971 + LRK53_RS05165 0.72 -0.3 1,213,971 + LRK53_RS05165 0.72 +1.3 1,213,971 + LRK53_RS05165 0.72 +0.7 1,213,972 - LRK53_RS05165 0.73 -0.3 1,213,972 - LRK53_RS05165 0.73 -0.3 1,213,972 - LRK53_RS05165 0.73 -1.9 1,213,972 - LRK53_RS05165 0.73 -1.0 1,213,972 - LRK53_RS05165 0.73 -0.2 1,213,986 + LRK53_RS05165 0.74 +0.3 1,213,987 - LRK53_RS05165 0.74 +0.1 1,213,987 - LRK53_RS05165 0.74 +0.3 1,213,987 - LRK53_RS05165 0.74 +0.3 1,214,074 - LRK53_RS05165 0.84 +0.3 1,214,074 - LRK53_RS05165 0.84 +0.3 1,214,140 - -1.0 1,214,222 + +0.2 1,214,222 + +0.8 1,214,222 + +1.3 1,214,222 + -0.3 1,214,223 - +0.6 1,214,223 - -0.9 1,214,223 - +0.9 1,214,223 - -1.9 1,214,223 - -0.3 1,214,400 + LRK53_RS05170 0.26 +1.3 1,214,400 + LRK53_RS05170 0.26 +0.3 1,214,400 + LRK53_RS05170 0.26 -1.3 1,214,401 - LRK53_RS05170 0.26 -1.0 1,214,401 - LRK53_RS05170 0.26 +1.7 1,214,401 - LRK53_RS05170 0.26 +1.3 1,214,731 - LRK53_RS05170 0.73 +0.3 1,214,731 - LRK53_RS05170 0.73 -0.7 1,214,762 + LRK53_RS05170 0.78 -2.4 1,214,913 + +2.3 1,214,914 - -2.3 1,214,914 - +1.3 1,214,914 - +0.1 1,214,995 + -0.6 1,214,996 - -0.9 1,214,996 - +0.1 1,215,185 - LRK53_RS05175 0.17 +0.7 1,215,358 + LRK53_RS05175 0.42 +1.7 1,215,358 + LRK53_RS05175 0.42 +0.3 1,215,358 + LRK53_RS05175 0.42 +1.3 1,215,359 - LRK53_RS05175 0.43 +3.3 1,215,359 - LRK53_RS05175 0.43 +0.9 1,215,359 - LRK53_RS05175 0.43 -0.5 1,215,647 - LRK53_RS05175 0.84 -0.3 1,215,746 - -0.7 1,215,776 + -1.5 1,215,776 + +0.1 1,215,776 + -1.0 1,215,776 + +2.3
Or see this region's nucleotide sequence