Experiment: R2A_PIPES; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt minE and minD are separated by 5 nucleotides minD and minC are separated by 37 nucleotides minC and OKGIIK_03315 overlap by 4 nucleotides OKGIIK_03315 and OKGIIK_03320 overlap by 4 nucleotides
OKGIIK_03300: minE - cell division topological specificity factor MinE, at 742,633 to 742,929
minE
OKGIIK_03305: minD - septum site-determining protein MinD, at 742,935 to 743,750
minD
OKGIIK_03310: minC - septum site-determining protein MinC, at 743,788 to 744,564
minC
OKGIIK_03315: OKGIIK_03315 - hypothetical protein, at 744,561 to 744,824
_03315
OKGIIK_03320: OKGIIK_03320 - GNAT family N-acetyltransferase, at 744,821 to 745,441
_03320
Position (kb) 743
744
745 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 743.032 kb on + strand, within minD at 743.033 kb on - strand, within minD at 743.200 kb on + strand, within minD at 743.348 kb on - strand, within minD at 743.348 kb on - strand, within minD at 743.402 kb on - strand, within minD at 743.736 kb on - strand at 743.781 kb on + strand at 743.781 kb on + strand at 743.781 kb on + strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.782 kb on - strand at 743.790 kb on - strand at 743.790 kb on - strand at 743.985 kb on - strand, within minC at 744.065 kb on + strand, within minC at 744.066 kb on - strand, within minC at 744.066 kb on - strand, within minC at 744.066 kb on - strand, within minC at 744.224 kb on + strand, within minC at 744.565 kb on - strand at 744.565 kb on - strand at 744.625 kb on + strand, within OKGIIK_03315 at 744.625 kb on + strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.626 kb on - strand, within OKGIIK_03315 at 744.634 kb on - strand, within OKGIIK_03315 at 744.634 kb on - strand, within OKGIIK_03315 at 744.634 kb on - strand, within OKGIIK_03315 at 744.634 kb on - strand, within OKGIIK_03315 at 744.634 kb on - strand, within OKGIIK_03315 at 744.760 kb on - strand, within OKGIIK_03315 at 744.760 kb on - strand, within OKGIIK_03315 at 744.760 kb on - strand, within OKGIIK_03315 at 744.794 kb on - strand, within OKGIIK_03315 at 744.794 kb on - strand, within OKGIIK_03315 at 744.794 kb on - strand, within OKGIIK_03315 at 744.803 kb on - strand at 744.949 kb on - strand, within OKGIIK_03320 at 744.949 kb on - strand, within OKGIIK_03320 at 744.949 kb on - strand, within OKGIIK_03320 at 745.029 kb on + strand, within OKGIIK_03320 at 745.029 kb on + strand, within OKGIIK_03320 at 745.030 kb on - strand, within OKGIIK_03320 at 745.030 kb on - strand, within OKGIIK_03320 at 745.030 kb on - strand, within OKGIIK_03320 at 745.030 kb on - strand, within OKGIIK_03320 at 745.114 kb on - strand, within OKGIIK_03320 at 745.114 kb on - strand, within OKGIIK_03320 at 745.132 kb on - strand, within OKGIIK_03320 at 745.207 kb on - strand, within OKGIIK_03320 at 745.228 kb on - strand, within OKGIIK_03320 at 745.311 kb on + strand, within OKGIIK_03320 at 745.326 kb on + strand, within OKGIIK_03320 at 745.327 kb on - strand, within OKGIIK_03320 at 745.327 kb on - strand, within OKGIIK_03320 at 745.332 kb on + strand, within OKGIIK_03320 at 745.333 kb on - strand, within OKGIIK_03320 at 745.395 kb on - strand at 745.395 kb on - strand at 745.504 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.527 kb on + strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.528 kb on - strand at 745.548 kb on + strand at 745.548 kb on + strand at 745.548 kb on + strand at 745.548 kb on + strand at 745.548 kb on + strand at 745.549 kb on - strand at 745.549 kb on - strand at 745.560 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES; no stress control remove 743,032 + minD OKGIIK_03305 0.12 -1.5 743,033 - minD OKGIIK_03305 0.12 -0.0 743,200 + minD OKGIIK_03305 0.32 -1.5 743,348 - minD OKGIIK_03305 0.51 -2.1 743,348 - minD OKGIIK_03305 0.51 -1.5 743,402 - minD OKGIIK_03305 0.57 -2.1 743,736 - -1.4 743,781 + -1.5 743,781 + -2.7 743,781 + -0.9 743,782 - -2.4 743,782 - -3.4 743,782 - -0.9 743,782 - -1.4 743,782 - -2.5 743,782 - -2.1 743,782 - -0.5 743,782 - -1.2 743,782 - -1.5 743,782 - -0.2 743,782 - -1.7 743,782 - -2.4 743,782 - -2.2 743,782 - -0.5 743,782 - -0.1 743,782 - -2.9 743,782 - -0.3 743,782 - -1.2 743,790 - +1.1 743,790 - -0.6 743,985 - minC OKGIIK_03310 0.25 +0.1 744,065 + minC OKGIIK_03310 0.36 +0.1 744,066 - minC OKGIIK_03310 0.36 -0.9 744,066 - minC OKGIIK_03310 0.36 -1.5 744,066 - minC OKGIIK_03310 0.36 -0.9 744,224 + minC OKGIIK_03310 0.56 -1.2 744,565 - -1.4 744,565 - -2.4 744,625 + OKGIIK_03315 0.24 -1.9 744,625 + OKGIIK_03315 0.24 +0.6 744,626 - OKGIIK_03315 0.25 +0.2 744,626 - OKGIIK_03315 0.25 +0.1 744,626 - OKGIIK_03315 0.25 -0.0 744,626 - OKGIIK_03315 0.25 +0.1 744,626 - OKGIIK_03315 0.25 +0.1 744,626 - OKGIIK_03315 0.25 -2.2 744,626 - OKGIIK_03315 0.25 -1.2 744,626 - OKGIIK_03315 0.25 -0.9 744,626 - OKGIIK_03315 0.25 -2.6 744,626 - OKGIIK_03315 0.25 +0.2 744,626 - OKGIIK_03315 0.25 -0.5 744,626 - OKGIIK_03315 0.25 -0.5 744,626 - OKGIIK_03315 0.25 +0.1 744,626 - OKGIIK_03315 0.25 +0.7 744,626 - OKGIIK_03315 0.25 -0.3 744,626 - OKGIIK_03315 0.25 -1.5 744,626 - OKGIIK_03315 0.25 -2.9 744,626 - OKGIIK_03315 0.25 +1.3 744,626 - OKGIIK_03315 0.25 -2.4 744,626 - OKGIIK_03315 0.25 -0.9 744,626 - OKGIIK_03315 0.25 -0.8 744,626 - OKGIIK_03315 0.25 +0.7 744,626 - OKGIIK_03315 0.25 -2.1 744,626 - OKGIIK_03315 0.25 -0.2 744,626 - OKGIIK_03315 0.25 -1.1 744,626 - OKGIIK_03315 0.25 -1.4 744,626 - OKGIIK_03315 0.25 -0.2 744,626 - OKGIIK_03315 0.25 -0.5 744,634 - OKGIIK_03315 0.28 -0.1 744,634 - OKGIIK_03315 0.28 -0.1 744,634 - OKGIIK_03315 0.28 -0.9 744,634 - OKGIIK_03315 0.28 -1.7 744,634 - OKGIIK_03315 0.28 -0.9 744,760 - OKGIIK_03315 0.75 +0.1 744,760 - OKGIIK_03315 0.75 -1.6 744,760 - OKGIIK_03315 0.75 -2.1 744,794 - OKGIIK_03315 0.88 +0.4 744,794 - OKGIIK_03315 0.88 -0.4 744,794 - OKGIIK_03315 0.88 +1.7 744,803 - -1.2 744,949 - OKGIIK_03320 0.21 +1.1 744,949 - OKGIIK_03320 0.21 -0.9 744,949 - OKGIIK_03320 0.21 +0.9 745,029 + OKGIIK_03320 0.33 -2.2 745,029 + OKGIIK_03320 0.33 -2.6 745,030 - OKGIIK_03320 0.34 -1.5 745,030 - OKGIIK_03320 0.34 +0.8 745,030 - OKGIIK_03320 0.34 -1.7 745,030 - OKGIIK_03320 0.34 -1.5 745,114 - OKGIIK_03320 0.47 -1.2 745,114 - OKGIIK_03320 0.47 -1.5 745,132 - OKGIIK_03320 0.50 -1.7 745,207 - OKGIIK_03320 0.62 -1.7 745,228 - OKGIIK_03320 0.66 -1.2 745,311 + OKGIIK_03320 0.79 -0.7 745,326 + OKGIIK_03320 0.81 -1.5 745,327 - OKGIIK_03320 0.81 -0.9 745,327 - OKGIIK_03320 0.81 -1.4 745,332 + OKGIIK_03320 0.82 -0.7 745,333 - OKGIIK_03320 0.82 -2.5 745,395 - -2.9 745,395 - -0.6 745,504 + -1.5 745,527 + -1.4 745,527 + -0.2 745,527 + -1.2 745,527 + -0.5 745,527 + -1.2 745,527 + -3.1 745,527 + -0.7 745,527 + -0.5 745,527 + -0.5 745,527 + +0.5 745,527 + -1.4 745,527 + -0.3 745,527 + -0.9 745,527 + +1.9 745,527 + -0.9 745,527 + -0.4 745,527 + +2.1 745,527 + +1.1 745,527 + +1.1 745,527 + +0.1 745,527 + -1.5 745,527 + -1.9 745,527 + -0.5 745,527 + -1.7 745,528 - -1.5 745,528 - -1.2 745,528 - -0.7 745,528 - -0.1 745,528 - -2.5 745,528 - -2.1 745,528 - -0.8 745,528 - +0.7 745,528 - -1.9 745,528 - -0.5 745,528 - -1.7 745,528 - -3.0 745,528 - -0.5 745,528 - -0.3 745,528 - -1.7 745,528 - -1.1 745,528 - -0.5 745,548 + -1.2 745,548 + -1.7 745,548 + -1.5 745,548 + -0.6 745,548 + -2.7 745,549 - -0.4 745,549 - -0.2 745,560 - -1.4
Or see this region's nucleotide sequence