Experiment: R2A_MES; pH5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aspS and OKGIIK_01825 are separated by 77 nucleotides OKGIIK_01825 and tACO1 are separated by 98 nucleotides tACO1 and ruvC are separated by 34 nucleotides ruvC and ruvA are separated by 25 nucleotides
OKGIIK_01820: aspS - aspartate--tRNA ligase, at 423,093 to 424,865
aspS
OKGIIK_01825: OKGIIK_01825 - AB hydrolase-1 domain-containing protein, at 424,943 to 425,578
_01825
OKGIIK_01830: tACO1 - YebC/PmpR family DNA-binding transcriptional regulator, at 425,677 to 426,411
tACO1
OKGIIK_01835: ruvC - crossover junction endodeoxyribonuclease RuvC, at 426,446 to 426,967
ruvC
OKGIIK_01840: ruvA - Holliday junction branch migration protein RuvA, at 426,993 to 427,589
ruvA
Position (kb)
425
426
427 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 425.051 kb on + strand, within OKGIIK_01825 at 425.051 kb on + strand, within OKGIIK_01825 at 425.051 kb on + strand, within OKGIIK_01825 at 425.051 kb on + strand, within OKGIIK_01825 at 425.051 kb on + strand, within OKGIIK_01825 at 425.051 kb on + strand, within OKGIIK_01825 at 425.051 kb on + strand, within OKGIIK_01825 at 425.051 kb on + strand, within OKGIIK_01825 at 425.052 kb on - strand, within OKGIIK_01825 at 425.052 kb on - strand, within OKGIIK_01825 at 425.052 kb on - strand, within OKGIIK_01825 at 425.052 kb on - strand, within OKGIIK_01825 at 425.052 kb on - strand, within OKGIIK_01825 at 425.682 kb on + strand at 425.682 kb on + strand at 425.683 kb on - strand at 425.926 kb on + strand, within tACO1 at 425.926 kb on + strand, within tACO1 at 425.926 kb on + strand, within tACO1 at 425.926 kb on + strand, within tACO1 at 426.409 kb on + strand at 426.410 kb on - strand at 427.098 kb on + strand, within ruvA at 427.098 kb on + strand, within ruvA at 427.099 kb on - strand, within ruvA
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_MES; pH5 remove 425,051 + OKGIIK_01825 0.17 -0.7 425,051 + OKGIIK_01825 0.17 -0.4 425,051 + OKGIIK_01825 0.17 -0.7 425,051 + OKGIIK_01825 0.17 -1.7 425,051 + OKGIIK_01825 0.17 +1.1 425,051 + OKGIIK_01825 0.17 -1.5 425,051 + OKGIIK_01825 0.17 -0.7 425,051 + OKGIIK_01825 0.17 +0.3 425,052 - OKGIIK_01825 0.17 +0.4 425,052 - OKGIIK_01825 0.17 -0.9 425,052 - OKGIIK_01825 0.17 -0.1 425,052 - OKGIIK_01825 0.17 +1.3 425,052 - OKGIIK_01825 0.17 -1.7 425,682 + -3.1 425,682 + -3.8 425,683 - -3.5 425,926 + tACO1 OKGIIK_01830 0.34 +1.3 425,926 + tACO1 OKGIIK_01830 0.34 -2.6 425,926 + tACO1 OKGIIK_01830 0.34 -4.2 425,926 + tACO1 OKGIIK_01830 0.34 -0.3 426,409 + +2.3 426,410 - -0.7 427,098 + ruvA OKGIIK_01840 0.18 -0.8 427,098 + ruvA OKGIIK_01840 0.18 +0.9 427,099 - ruvA OKGIIK_01840 0.18 +1.3
Or see this region's nucleotide sequence