Experiment: R2A_PIPES with Metal_ion_mix_sitestressplate 2 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Gly_trna and Tyr_trna are separated by 18 nucleotides Tyr_trna and yciA are separated by 93 nucleotides yciA and OKGIIK_10565 are separated by 140 nucleotides OKGIIK_10565 and pth are separated by 96 nucleotides pth and rplY are separated by 54 nucleotides
OKGIIK_10550: Gly_trna - tRNA-Gly, at 2,266,946 to 2,267,019
_trna
OKGIIK_10555: Tyr_trna - tRNA-Tyr, at 2,267,038 to 2,267,123
_trna
OKGIIK_10560: yciA - acyl-CoA thioesterase, at 2,267,217 to 2,267,705
yciA
OKGIIK_10565: OKGIIK_10565 - Ribosome-binding ATPase YchF, at 2,267,846 to 2,268,934
_10565
OKGIIK_10570: pth - aminoacyl-tRNA hydrolase, at 2,269,031 to 2,269,606
pth
OKGIIK_10575: rplY - 50S ribosomal protein L25/general stress protein Ctc, at 2,269,661 to 2,270,332
rplY
Position (kb)
2267
2268
2269 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2266.939 kb on - strand at 2266.939 kb on - strand at 2266.939 kb on - strand at 2266.939 kb on - strand at 2266.939 kb on - strand at 2266.939 kb on - strand at 2266.939 kb on - strand at 2267.292 kb on + strand, within yciA at 2267.293 kb on - strand, within yciA at 2267.293 kb on - strand, within yciA at 2267.293 kb on - strand, within yciA at 2267.293 kb on - strand, within yciA at 2267.293 kb on - strand, within yciA at 2267.293 kb on - strand, within yciA at 2267.293 kb on - strand, within yciA at 2267.293 kb on - strand, within yciA at 2267.560 kb on + strand, within yciA at 2267.560 kb on + strand, within yciA at 2267.560 kb on + strand, within yciA at 2267.560 kb on + strand, within yciA at 2267.560 kb on + strand, within yciA at 2267.560 kb on + strand, within yciA at 2267.560 kb on + strand, within yciA at 2267.560 kb on + strand, within yciA at 2267.560 kb on + strand, within yciA at 2267.561 kb on - strand, within yciA at 2267.561 kb on - strand, within yciA at 2267.561 kb on - strand, within yciA at 2267.561 kb on - strand, within yciA at 2267.561 kb on - strand, within yciA at 2267.590 kb on + strand, within yciA at 2267.696 kb on + strand at 2267.696 kb on + strand at 2267.697 kb on - strand at 2267.697 kb on - strand at 2267.743 kb on + strand at 2267.743 kb on + strand at 2267.743 kb on + strand at 2267.743 kb on + strand at 2267.743 kb on + strand at 2267.743 kb on + strand at 2267.743 kb on + strand at 2267.743 kb on + strand at 2267.743 kb on + strand at 2267.743 kb on + strand at 2267.743 kb on + strand at 2267.743 kb on + strand at 2267.743 kb on + strand at 2267.743 kb on + strand at 2267.744 kb on - strand at 2267.744 kb on - strand at 2267.744 kb on - strand at 2267.744 kb on - strand at 2267.744 kb on - strand at 2267.744 kb on - strand at 2267.744 kb on - strand at 2267.744 kb on - strand at 2267.744 kb on - strand at 2267.744 kb on - strand at 2267.744 kb on - strand at 2267.744 kb on - strand at 2267.744 kb on - strand at 2267.744 kb on - strand at 2267.744 kb on - strand at 2267.744 kb on - strand at 2267.744 kb on - strand at 2267.744 kb on - strand at 2267.744 kb on - strand at 2267.892 kb on + strand at 2267.892 kb on + strand at 2267.892 kb on + strand at 2267.892 kb on + strand at 2267.892 kb on + strand at 2267.892 kb on + strand at 2267.892 kb on + strand at 2267.892 kb on + strand at 2267.892 kb on + strand at 2267.893 kb on - strand at 2267.893 kb on - strand at 2267.893 kb on - strand at 2267.893 kb on - strand at 2267.893 kb on - strand at 2267.893 kb on - strand at 2267.893 kb on - strand at 2267.893 kb on - strand at 2267.893 kb on - strand at 2267.893 kb on - strand at 2267.893 kb on - strand at 2267.893 kb on - strand at 2267.949 kb on + strand at 2267.949 kb on + strand at 2267.950 kb on - strand at 2267.950 kb on - strand at 2267.950 kb on - strand at 2267.950 kb on - strand at 2267.950 kb on - strand at 2267.950 kb on - strand at 2268.093 kb on + strand, within OKGIIK_10565 at 2268.093 kb on + strand, within OKGIIK_10565 at 2268.093 kb on + strand, within OKGIIK_10565 at 2268.093 kb on + strand, within OKGIIK_10565 at 2268.093 kb on + strand, within OKGIIK_10565 at 2268.093 kb on + strand, within OKGIIK_10565 at 2268.093 kb on + strand, within OKGIIK_10565 at 2268.094 kb on - strand, within OKGIIK_10565 at 2268.094 kb on - strand, within OKGIIK_10565 at 2268.094 kb on - strand, within OKGIIK_10565 at 2268.118 kb on - strand, within OKGIIK_10565 at 2268.327 kb on + strand, within OKGIIK_10565 at 2268.327 kb on + strand, within OKGIIK_10565 at 2268.327 kb on + strand, within OKGIIK_10565 at 2268.327 kb on + strand, within OKGIIK_10565 at 2268.327 kb on + strand, within OKGIIK_10565 at 2268.327 kb on + strand, within OKGIIK_10565 at 2268.327 kb on + strand, within OKGIIK_10565 at 2268.327 kb on + strand, within OKGIIK_10565 at 2268.327 kb on + strand, within OKGIIK_10565 at 2268.327 kb on + strand, within OKGIIK_10565 at 2268.328 kb on - strand, within OKGIIK_10565 at 2268.328 kb on - strand, within OKGIIK_10565 at 2268.328 kb on - strand, within OKGIIK_10565 at 2268.328 kb on - strand, within OKGIIK_10565 at 2268.328 kb on - strand, within OKGIIK_10565 at 2268.328 kb on - strand, within OKGIIK_10565 at 2268.328 kb on - strand, within OKGIIK_10565 at 2268.328 kb on - strand, within OKGIIK_10565 at 2268.328 kb on - strand, within OKGIIK_10565 at 2268.328 kb on - strand, within OKGIIK_10565 at 2268.328 kb on - strand, within OKGIIK_10565 at 2268.328 kb on - strand, within OKGIIK_10565 at 2268.328 kb on - strand, within OKGIIK_10565 at 2268.328 kb on - strand, within OKGIIK_10565 at 2268.328 kb on - strand, within OKGIIK_10565 at 2268.328 kb on - strand, within OKGIIK_10565 at 2268.656 kb on - strand, within OKGIIK_10565 at 2268.656 kb on - strand, within OKGIIK_10565 at 2268.990 kb on - strand at 2268.991 kb on + strand at 2268.991 kb on + strand at 2268.991 kb on + strand at 2268.991 kb on + strand at 2268.991 kb on + strand at 2268.991 kb on + strand at 2268.991 kb on + strand at 2268.992 kb on - strand at 2268.992 kb on - strand at 2268.992 kb on - strand at 2268.992 kb on - strand at 2268.997 kb on + strand at 2268.997 kb on + strand at 2268.998 kb on - strand at 2268.998 kb on - strand at 2268.998 kb on - strand at 2268.998 kb on - strand at 2268.998 kb on - strand at 2269.032 kb on + strand at 2269.032 kb on + strand at 2269.032 kb on + strand at 2269.032 kb on + strand at 2269.039 kb on + strand at 2269.039 kb on + strand at 2269.039 kb on + strand at 2269.039 kb on + strand at 2269.040 kb on - strand at 2269.040 kb on - strand at 2269.040 kb on - strand at 2269.040 kb on - strand at 2269.040 kb on - strand at 2269.040 kb on - strand at 2269.040 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Metal_ion_mix_sitestressplate 2 x remove 2,266,939 - +1.2 2,266,939 - -0.4 2,266,939 - -1.5 2,266,939 - -2.1 2,266,939 - -0.5 2,266,939 - -1.5 2,266,939 - -1.2 2,267,292 + yciA OKGIIK_10560 0.15 -1.5 2,267,293 - yciA OKGIIK_10560 0.16 -1.2 2,267,293 - yciA OKGIIK_10560 0.16 -0.3 2,267,293 - yciA OKGIIK_10560 0.16 -0.8 2,267,293 - yciA OKGIIK_10560 0.16 -1.5 2,267,293 - yciA OKGIIK_10560 0.16 -1.7 2,267,293 - yciA OKGIIK_10560 0.16 -0.7 2,267,293 - yciA OKGIIK_10560 0.16 +0.1 2,267,293 - yciA OKGIIK_10560 0.16 +0.5 2,267,560 + yciA OKGIIK_10560 0.70 +0.1 2,267,560 + yciA OKGIIK_10560 0.70 -0.9 2,267,560 + yciA OKGIIK_10560 0.70 -2.5 2,267,560 + yciA OKGIIK_10560 0.70 -1.2 2,267,560 + yciA OKGIIK_10560 0.70 -0.3 2,267,560 + yciA OKGIIK_10560 0.70 -0.9 2,267,560 + yciA OKGIIK_10560 0.70 +0.0 2,267,560 + yciA OKGIIK_10560 0.70 -0.5 2,267,560 + yciA OKGIIK_10560 0.70 +0.2 2,267,561 - yciA OKGIIK_10560 0.70 +0.5 2,267,561 - yciA OKGIIK_10560 0.70 +0.5 2,267,561 - yciA OKGIIK_10560 0.70 -1.7 2,267,561 - yciA OKGIIK_10560 0.70 -2.1 2,267,561 - yciA OKGIIK_10560 0.70 -0.8 2,267,590 + yciA OKGIIK_10560 0.76 +0.1 2,267,696 + -0.9 2,267,696 + -1.9 2,267,697 - -0.2 2,267,697 - -2.6 2,267,743 + -0.9 2,267,743 + -2.7 2,267,743 + -1.9 2,267,743 + -0.6 2,267,743 + -0.7 2,267,743 + -2.3 2,267,743 + +0.7 2,267,743 + -3.7 2,267,743 + -2.4 2,267,743 + -2.2 2,267,743 + -0.4 2,267,743 + -0.5 2,267,743 + -0.3 2,267,743 + -0.3 2,267,744 - +0.7 2,267,744 - +0.1 2,267,744 - +2.1 2,267,744 - -2.1 2,267,744 - +0.4 2,267,744 - -1.5 2,267,744 - +0.2 2,267,744 - +0.4 2,267,744 - -0.4 2,267,744 - +0.4 2,267,744 - -1.2 2,267,744 - -0.2 2,267,744 - -0.2 2,267,744 - -3.6 2,267,744 - +1.4 2,267,744 - -2.1 2,267,744 - -0.7 2,267,744 - -0.9 2,267,744 - -1.2 2,267,892 + -1.5 2,267,892 + -1.1 2,267,892 + -1.7 2,267,892 + -1.2 2,267,892 + +1.1 2,267,892 + +0.8 2,267,892 + +1.3 2,267,892 + -1.4 2,267,892 + -0.8 2,267,893 - +1.1 2,267,893 - -1.5 2,267,893 - -1.2 2,267,893 - +1.1 2,267,893 - -3.0 2,267,893 - -1.7 2,267,893 - -2.3 2,267,893 - -1.7 2,267,893 - -1.7 2,267,893 - -0.6 2,267,893 - -2.7 2,267,893 - +1.1 2,267,949 + +1.1 2,267,949 + -1.5 2,267,950 - -1.9 2,267,950 - -2.7 2,267,950 - -1.5 2,267,950 - -3.1 2,267,950 - -0.8 2,267,950 - -2.1 2,268,093 + OKGIIK_10565 0.23 -2.5 2,268,093 + OKGIIK_10565 0.23 -0.5 2,268,093 + OKGIIK_10565 0.23 -0.9 2,268,093 + OKGIIK_10565 0.23 -4.0 2,268,093 + OKGIIK_10565 0.23 +1.1 2,268,093 + OKGIIK_10565 0.23 -2.2 2,268,093 + OKGIIK_10565 0.23 +2.1 2,268,094 - OKGIIK_10565 0.23 -2.2 2,268,094 - OKGIIK_10565 0.23 -1.9 2,268,094 - OKGIIK_10565 0.23 +2.1 2,268,118 - OKGIIK_10565 0.25 -0.9 2,268,327 + OKGIIK_10565 0.44 -3.3 2,268,327 + OKGIIK_10565 0.44 -1.1 2,268,327 + OKGIIK_10565 0.44 +1.1 2,268,327 + OKGIIK_10565 0.44 -0.7 2,268,327 + OKGIIK_10565 0.44 -2.8 2,268,327 + OKGIIK_10565 0.44 -1.1 2,268,327 + OKGIIK_10565 0.44 +1.1 2,268,327 + OKGIIK_10565 0.44 +1.1 2,268,327 + OKGIIK_10565 0.44 +0.5 2,268,327 + OKGIIK_10565 0.44 -1.1 2,268,328 - OKGIIK_10565 0.44 -0.8 2,268,328 - OKGIIK_10565 0.44 +0.1 2,268,328 - OKGIIK_10565 0.44 -0.5 2,268,328 - OKGIIK_10565 0.44 -2.8 2,268,328 - OKGIIK_10565 0.44 -0.5 2,268,328 - OKGIIK_10565 0.44 -2.1 2,268,328 - OKGIIK_10565 0.44 +0.1 2,268,328 - OKGIIK_10565 0.44 -0.3 2,268,328 - OKGIIK_10565 0.44 +0.1 2,268,328 - OKGIIK_10565 0.44 -1.9 2,268,328 - OKGIIK_10565 0.44 -0.7 2,268,328 - OKGIIK_10565 0.44 -0.5 2,268,328 - OKGIIK_10565 0.44 -0.1 2,268,328 - OKGIIK_10565 0.44 -1.9 2,268,328 - OKGIIK_10565 0.44 -2.2 2,268,328 - OKGIIK_10565 0.44 -0.9 2,268,656 - OKGIIK_10565 0.74 -0.1 2,268,656 - OKGIIK_10565 0.74 -2.6 2,268,990 - -0.5 2,268,991 + -0.6 2,268,991 + -1.8 2,268,991 + +2.4 2,268,991 + -0.9 2,268,991 + -1.1 2,268,991 + -0.5 2,268,991 + +2.1 2,268,992 - -0.6 2,268,992 - +0.1 2,268,992 - +0.5 2,268,992 - -1.0 2,268,997 + -0.7 2,268,997 + -4.1 2,268,998 - -0.3 2,268,998 - +1.5 2,268,998 - -0.1 2,268,998 - +0.1 2,268,998 - -1.9 2,269,032 + -1.5 2,269,032 + -0.5 2,269,032 + -1.1 2,269,032 + +0.1 2,269,039 + -1.2 2,269,039 + -1.7 2,269,039 + -0.5 2,269,039 + -3.3 2,269,040 - -1.2 2,269,040 - -0.8 2,269,040 - -0.9 2,269,040 - -2.1 2,269,040 - -0.9 2,269,040 - -1.3 2,269,040 - +0.5
Or see this region's nucleotide sequence