Experiment: R2A_PIPES with Metal_ion_mix_sitestressplate 2 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfbD and rfbC are separated by 143 nucleotides rfbC and rfbA overlap by 4 nucleotides rfbA and rfbB overlap by 4 nucleotides
OKGIIK_01160: rfbD - dTDP-4-dehydrorhamnose reductase, at 250,252 to 251,187
rfbD
OKGIIK_01165: rfbC - dTDP-4-dehydrorhamnose 3,5-epimerase, at 251,331 to 251,888
rfbC
OKGIIK_01170: rfbA - glucose-1-phosphate thymidylyltransferase RfbA, at 251,885 to 252,769
rfbA
OKGIIK_01175: rfbB - dTDP-glucose 4,6-dehydratase, at 252,766 to 253,836
rfbB
Position (kb) 251
252 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 250.348 kb on - strand, within rfbD at 250.701 kb on - strand, within rfbD at 250.701 kb on - strand, within rfbD at 250.781 kb on + strand, within rfbD at 250.817 kb on + strand, within rfbD at 250.817 kb on + strand, within rfbD at 250.817 kb on + strand, within rfbD at 250.847 kb on + strand, within rfbD at 250.847 kb on + strand, within rfbD at 250.847 kb on + strand, within rfbD at 250.847 kb on + strand, within rfbD at 250.847 kb on + strand, within rfbD at 250.847 kb on + strand, within rfbD at 250.848 kb on - strand, within rfbD at 250.848 kb on - strand, within rfbD at 250.848 kb on - strand, within rfbD at 250.848 kb on - strand, within rfbD at 250.848 kb on - strand, within rfbD at 250.848 kb on - strand, within rfbD at 250.859 kb on + strand, within rfbD at 250.859 kb on + strand, within rfbD at 250.859 kb on + strand, within rfbD at 250.859 kb on + strand, within rfbD at 250.860 kb on - strand, within rfbD at 250.860 kb on - strand, within rfbD at 250.860 kb on - strand, within rfbD at 250.860 kb on - strand, within rfbD at 250.974 kb on - strand, within rfbD at 250.974 kb on - strand, within rfbD at 250.974 kb on - strand, within rfbD at 251.176 kb on + strand at 251.176 kb on + strand at 251.176 kb on + strand at 251.176 kb on + strand at 251.176 kb on + strand at 251.176 kb on + strand at 251.176 kb on + strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.203 kb on + strand at 251.203 kb on + strand at 251.203 kb on + strand at 251.332 kb on + strand at 251.333 kb on - strand at 251.333 kb on - strand at 251.341 kb on + strand at 251.341 kb on + strand at 251.341 kb on + strand at 251.341 kb on + strand at 251.341 kb on + strand at 251.342 kb on - strand at 251.342 kb on - strand at 251.342 kb on - strand at 251.342 kb on - strand at 251.482 kb on + strand, within rfbC at 251.482 kb on + strand, within rfbC at 251.483 kb on - strand, within rfbC at 251.483 kb on - strand, within rfbC at 251.500 kb on + strand, within rfbC at 251.500 kb on + strand, within rfbC at 251.500 kb on + strand, within rfbC at 251.500 kb on + strand, within rfbC at 251.500 kb on + strand, within rfbC at 251.501 kb on - strand, within rfbC at 251.501 kb on - strand, within rfbC at 251.501 kb on - strand, within rfbC at 251.633 kb on + strand, within rfbC at 251.650 kb on + strand, within rfbC at 251.651 kb on - strand, within rfbC at 251.651 kb on - strand, within rfbC at 251.651 kb on - strand, within rfbC at 251.704 kb on + strand, within rfbC at 251.704 kb on + strand, within rfbC at 251.704 kb on + strand, within rfbC at 251.705 kb on - strand, within rfbC at 251.705 kb on - strand, within rfbC at 251.705 kb on - strand, within rfbC at 251.754 kb on + strand, within rfbC at 251.800 kb on + strand, within rfbC at 251.800 kb on + strand, within rfbC at 251.800 kb on + strand, within rfbC at 251.800 kb on + strand, within rfbC at 251.800 kb on + strand, within rfbC at 251.801 kb on - strand, within rfbC at 251.809 kb on + strand, within rfbC at 251.809 kb on + strand, within rfbC at 251.810 kb on - strand, within rfbC at 251.810 kb on - strand, within rfbC at 251.922 kb on + strand at 251.922 kb on + strand at 251.923 kb on - strand at 251.923 kb on - strand at 251.998 kb on - strand, within rfbA at 252.057 kb on + strand, within rfbA at 252.057 kb on + strand, within rfbA at 252.057 kb on + strand, within rfbA at 252.057 kb on + strand, within rfbA at 252.057 kb on + strand, within rfbA at 252.057 kb on + strand, within rfbA at 252.058 kb on - strand, within rfbA at 252.058 kb on - strand, within rfbA at 252.058 kb on - strand, within rfbA at 252.058 kb on - strand, within rfbA at 252.058 kb on - strand, within rfbA at 252.058 kb on - strand, within rfbA at 252.058 kb on - strand, within rfbA at 252.058 kb on - strand, within rfbA at 252.153 kb on + strand, within rfbA at 252.153 kb on + strand, within rfbA at 252.153 kb on + strand, within rfbA at 252.153 kb on + strand, within rfbA at 252.154 kb on - strand, within rfbA at 252.154 kb on - strand, within rfbA at 252.154 kb on - strand, within rfbA at 252.216 kb on + strand, within rfbA at 252.216 kb on + strand, within rfbA at 252.217 kb on - strand, within rfbA at 252.217 kb on - strand, within rfbA at 252.217 kb on - strand, within rfbA at 252.217 kb on - strand, within rfbA at 252.217 kb on - strand, within rfbA at 252.243 kb on + strand, within rfbA at 252.243 kb on + strand, within rfbA at 252.243 kb on + strand, within rfbA at 252.243 kb on + strand, within rfbA at 252.243 kb on + strand, within rfbA at 252.243 kb on + strand, within rfbA at 252.243 kb on + strand, within rfbA at 252.244 kb on - strand, within rfbA at 252.244 kb on - strand, within rfbA at 252.244 kb on - strand, within rfbA at 252.244 kb on - strand, within rfbA at 252.244 kb on - strand, within rfbA at 252.244 kb on - strand, within rfbA at 252.244 kb on - strand, within rfbA at 252.244 kb on - strand, within rfbA at 252.244 kb on - strand, within rfbA at 252.244 kb on - strand, within rfbA at 252.244 kb on - strand, within rfbA at 252.244 kb on - strand, within rfbA at 252.261 kb on + strand, within rfbA at 252.261 kb on + strand, within rfbA at 252.354 kb on - strand, within rfbA at 252.361 kb on - strand, within rfbA at 252.429 kb on + strand, within rfbA at 252.430 kb on - strand, within rfbA at 252.430 kb on - strand, within rfbA at 252.430 kb on - strand, within rfbA at 252.534 kb on + strand, within rfbA at 252.535 kb on - strand, within rfbA at 252.535 kb on - strand, within rfbA at 252.657 kb on + strand, within rfbA at 252.657 kb on + strand, within rfbA at 252.660 kb on + strand, within rfbA at 252.660 kb on + strand, within rfbA at 252.660 kb on + strand, within rfbA at 252.660 kb on + strand, within rfbA at 252.660 kb on + strand, within rfbA at 252.660 kb on + strand, within rfbA at 252.660 kb on + strand, within rfbA at 252.660 kb on + strand, within rfbA at 252.660 kb on + strand, within rfbA at 252.660 kb on + strand, within rfbA at 252.660 kb on + strand, within rfbA at 252.661 kb on - strand, within rfbA at 252.661 kb on - strand, within rfbA at 252.661 kb on - strand, within rfbA at 252.661 kb on - strand, within rfbA at 252.661 kb on - strand, within rfbA at 252.661 kb on - strand, within rfbA at 252.661 kb on - strand, within rfbA at 252.661 kb on - strand, within rfbA at 252.661 kb on - strand, within rfbA at 252.661 kb on - strand, within rfbA at 252.661 kb on - strand, within rfbA at 252.661 kb on - strand, within rfbA at 252.661 kb on - strand, within rfbA at 252.661 kb on - strand, within rfbA at 252.661 kb on - strand, within rfbA at 252.661 kb on - strand, within rfbA at 252.678 kb on + strand, within rfbA at 252.678 kb on + strand, within rfbA at 252.679 kb on - strand, within rfbA at 252.679 kb on - strand, within rfbA at 252.679 kb on - strand, within rfbA at 252.791 kb on + strand at 252.791 kb on + strand at 252.792 kb on - strand at 252.792 kb on - strand at 252.792 kb on - strand at 252.792 kb on - strand at 252.836 kb on + strand at 252.836 kb on + strand at 252.836 kb on + strand at 252.836 kb on + strand at 252.836 kb on + strand at 252.837 kb on - strand at 252.837 kb on - strand at 252.837 kb on - strand at 252.837 kb on - strand at 252.837 kb on - strand at 252.837 kb on - strand at 252.837 kb on - strand at 252.837 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Metal_ion_mix_sitestressplate 2 x remove 250,348 - rfbD OKGIIK_01160 0.10 -2.1 250,701 - rfbD OKGIIK_01160 0.48 -1.7 250,701 - rfbD OKGIIK_01160 0.48 -1.9 250,781 + rfbD OKGIIK_01160 0.57 -1.3 250,817 + rfbD OKGIIK_01160 0.60 -2.4 250,817 + rfbD OKGIIK_01160 0.60 +0.1 250,817 + rfbD OKGIIK_01160 0.60 -3.3 250,847 + rfbD OKGIIK_01160 0.64 -3.0 250,847 + rfbD OKGIIK_01160 0.64 -2.1 250,847 + rfbD OKGIIK_01160 0.64 -2.6 250,847 + rfbD OKGIIK_01160 0.64 -1.7 250,847 + rfbD OKGIIK_01160 0.64 -1.9 250,847 + rfbD OKGIIK_01160 0.64 -0.9 250,848 - rfbD OKGIIK_01160 0.64 +0.3 250,848 - rfbD OKGIIK_01160 0.64 +0.7 250,848 - rfbD OKGIIK_01160 0.64 -1.9 250,848 - rfbD OKGIIK_01160 0.64 -2.6 250,848 - rfbD OKGIIK_01160 0.64 -1.5 250,848 - rfbD OKGIIK_01160 0.64 -1.8 250,859 + rfbD OKGIIK_01160 0.65 -0.5 250,859 + rfbD OKGIIK_01160 0.65 -2.6 250,859 + rfbD OKGIIK_01160 0.65 -1.0 250,859 + rfbD OKGIIK_01160 0.65 -0.5 250,860 - rfbD OKGIIK_01160 0.65 -1.3 250,860 - rfbD OKGIIK_01160 0.65 -1.2 250,860 - rfbD OKGIIK_01160 0.65 -2.9 250,860 - rfbD OKGIIK_01160 0.65 +1.1 250,974 - rfbD OKGIIK_01160 0.77 -0.9 250,974 - rfbD OKGIIK_01160 0.77 -1.3 250,974 - rfbD OKGIIK_01160 0.77 -1.9 251,176 + -1.3 251,176 + -0.5 251,176 + -0.3 251,176 + -0.3 251,176 + -0.7 251,176 + +0.7 251,176 + -0.5 251,177 - -0.3 251,177 - -0.9 251,177 - -1.5 251,177 - -0.5 251,177 - -0.3 251,177 - -1.4 251,177 - -0.1 251,177 - +2.1 251,177 - -0.5 251,177 - +0.7 251,177 - -2.1 251,203 + -2.1 251,203 + -0.8 251,203 + -0.1 251,332 + -0.5 251,333 - -0.6 251,333 - -0.3 251,341 + +0.7 251,341 + -0.1 251,341 + +0.3 251,341 + -0.9 251,341 + -0.3 251,342 - -2.7 251,342 - -2.5 251,342 - -2.1 251,342 - -0.5 251,482 + rfbC OKGIIK_01165 0.27 -2.4 251,482 + rfbC OKGIIK_01165 0.27 -1.9 251,483 - rfbC OKGIIK_01165 0.27 -0.9 251,483 - rfbC OKGIIK_01165 0.27 -1.4 251,500 + rfbC OKGIIK_01165 0.30 -2.3 251,500 + rfbC OKGIIK_01165 0.30 -1.5 251,500 + rfbC OKGIIK_01165 0.30 -0.9 251,500 + rfbC OKGIIK_01165 0.30 -0.5 251,500 + rfbC OKGIIK_01165 0.30 -3.8 251,501 - rfbC OKGIIK_01165 0.30 +1.1 251,501 - rfbC OKGIIK_01165 0.30 -1.9 251,501 - rfbC OKGIIK_01165 0.30 -1.3 251,633 + rfbC OKGIIK_01165 0.54 -2.1 251,650 + rfbC OKGIIK_01165 0.57 -0.3 251,651 - rfbC OKGIIK_01165 0.57 +1.1 251,651 - rfbC OKGIIK_01165 0.57 -0.9 251,651 - rfbC OKGIIK_01165 0.57 -1.3 251,704 + rfbC OKGIIK_01165 0.67 +0.1 251,704 + rfbC OKGIIK_01165 0.67 +1.1 251,704 + rfbC OKGIIK_01165 0.67 -2.3 251,705 - rfbC OKGIIK_01165 0.67 -3.1 251,705 - rfbC OKGIIK_01165 0.67 -1.5 251,705 - rfbC OKGIIK_01165 0.67 -2.3 251,754 + rfbC OKGIIK_01165 0.76 -2.7 251,800 + rfbC OKGIIK_01165 0.84 -2.5 251,800 + rfbC OKGIIK_01165 0.84 -2.1 251,800 + rfbC OKGIIK_01165 0.84 -2.8 251,800 + rfbC OKGIIK_01165 0.84 -0.5 251,800 + rfbC OKGIIK_01165 0.84 -1.7 251,801 - rfbC OKGIIK_01165 0.84 -3.6 251,809 + rfbC OKGIIK_01165 0.86 -1.7 251,809 + rfbC OKGIIK_01165 0.86 -2.0 251,810 - rfbC OKGIIK_01165 0.86 -0.8 251,810 - rfbC OKGIIK_01165 0.86 -1.7 251,922 + -2.9 251,922 + -1.9 251,923 - -0.1 251,923 - -2.8 251,998 - rfbA OKGIIK_01170 0.13 -0.3 252,057 + rfbA OKGIIK_01170 0.19 -0.9 252,057 + rfbA OKGIIK_01170 0.19 -0.5 252,057 + rfbA OKGIIK_01170 0.19 -2.7 252,057 + rfbA OKGIIK_01170 0.19 -2.1 252,057 + rfbA OKGIIK_01170 0.19 -1.5 252,057 + rfbA OKGIIK_01170 0.19 -0.5 252,058 - rfbA OKGIIK_01170 0.20 +1.7 252,058 - rfbA OKGIIK_01170 0.20 -1.7 252,058 - rfbA OKGIIK_01170 0.20 -0.9 252,058 - rfbA OKGIIK_01170 0.20 -1.7 252,058 - rfbA OKGIIK_01170 0.20 -0.5 252,058 - rfbA OKGIIK_01170 0.20 -0.7 252,058 - rfbA OKGIIK_01170 0.20 -1.7 252,058 - rfbA OKGIIK_01170 0.20 -1.9 252,153 + rfbA OKGIIK_01170 0.30 -0.9 252,153 + rfbA OKGIIK_01170 0.30 +2.1 252,153 + rfbA OKGIIK_01170 0.30 -0.5 252,153 + rfbA OKGIIK_01170 0.30 -2.6 252,154 - rfbA OKGIIK_01170 0.30 -1.1 252,154 - rfbA OKGIIK_01170 0.30 -0.4 252,154 - rfbA OKGIIK_01170 0.30 +1.3 252,216 + rfbA OKGIIK_01170 0.37 -0.9 252,216 + rfbA OKGIIK_01170 0.37 -2.3 252,217 - rfbA OKGIIK_01170 0.38 -2.6 252,217 - rfbA OKGIIK_01170 0.38 -1.9 252,217 - rfbA OKGIIK_01170 0.38 -1.7 252,217 - rfbA OKGIIK_01170 0.38 -1.5 252,217 - rfbA OKGIIK_01170 0.38 +0.4 252,243 + rfbA OKGIIK_01170 0.40 -0.9 252,243 + rfbA OKGIIK_01170 0.40 -1.9 252,243 + rfbA OKGIIK_01170 0.40 -0.6 252,243 + rfbA OKGIIK_01170 0.40 -1.5 252,243 + rfbA OKGIIK_01170 0.40 -1.1 252,243 + rfbA OKGIIK_01170 0.40 +0.2 252,243 + rfbA OKGIIK_01170 0.40 +0.7 252,244 - rfbA OKGIIK_01170 0.41 -1.8 252,244 - rfbA OKGIIK_01170 0.41 -2.8 252,244 - rfbA OKGIIK_01170 0.41 -1.6 252,244 - rfbA OKGIIK_01170 0.41 -2.3 252,244 - rfbA OKGIIK_01170 0.41 -2.4 252,244 - rfbA OKGIIK_01170 0.41 +0.3 252,244 - rfbA OKGIIK_01170 0.41 +1.1 252,244 - rfbA OKGIIK_01170 0.41 -0.7 252,244 - rfbA OKGIIK_01170 0.41 -2.1 252,244 - rfbA OKGIIK_01170 0.41 -1.7 252,244 - rfbA OKGIIK_01170 0.41 -2.6 252,244 - rfbA OKGIIK_01170 0.41 -2.3 252,261 + rfbA OKGIIK_01170 0.42 +0.5 252,261 + rfbA OKGIIK_01170 0.42 -0.2 252,354 - rfbA OKGIIK_01170 0.53 +0.4 252,361 - rfbA OKGIIK_01170 0.54 +0.4 252,429 + rfbA OKGIIK_01170 0.61 -1.5 252,430 - rfbA OKGIIK_01170 0.62 -0.9 252,430 - rfbA OKGIIK_01170 0.62 -1.0 252,430 - rfbA OKGIIK_01170 0.62 +0.7 252,534 + rfbA OKGIIK_01170 0.73 -1.9 252,535 - rfbA OKGIIK_01170 0.73 -2.1 252,535 - rfbA OKGIIK_01170 0.73 -0.5 252,657 + rfbA OKGIIK_01170 0.87 -1.5 252,657 + rfbA OKGIIK_01170 0.87 -1.7 252,660 + rfbA OKGIIK_01170 0.88 +1.1 252,660 + rfbA OKGIIK_01170 0.88 -2.2 252,660 + rfbA OKGIIK_01170 0.88 -5.6 252,660 + rfbA OKGIIK_01170 0.88 -1.3 252,660 + rfbA OKGIIK_01170 0.88 +0.5 252,660 + rfbA OKGIIK_01170 0.88 -2.6 252,660 + rfbA OKGIIK_01170 0.88 -0.5 252,660 + rfbA OKGIIK_01170 0.88 -2.1 252,660 + rfbA OKGIIK_01170 0.88 +0.4 252,660 + rfbA OKGIIK_01170 0.88 +0.1 252,660 + rfbA OKGIIK_01170 0.88 +1.1 252,661 - rfbA OKGIIK_01170 0.88 -0.7 252,661 - rfbA OKGIIK_01170 0.88 +1.1 252,661 - rfbA OKGIIK_01170 0.88 -0.9 252,661 - rfbA OKGIIK_01170 0.88 -1.9 252,661 - rfbA OKGIIK_01170 0.88 -0.9 252,661 - rfbA OKGIIK_01170 0.88 -1.3 252,661 - rfbA OKGIIK_01170 0.88 -3.1 252,661 - rfbA OKGIIK_01170 0.88 -0.5 252,661 - rfbA OKGIIK_01170 0.88 -1.5 252,661 - rfbA OKGIIK_01170 0.88 -2.7 252,661 - rfbA OKGIIK_01170 0.88 -1.3 252,661 - rfbA OKGIIK_01170 0.88 +0.1 252,661 - rfbA OKGIIK_01170 0.88 -0.7 252,661 - rfbA OKGIIK_01170 0.88 +0.3 252,661 - rfbA OKGIIK_01170 0.88 -1.3 252,661 - rfbA OKGIIK_01170 0.88 -0.9 252,678 + rfbA OKGIIK_01170 0.90 -2.7 252,678 + rfbA OKGIIK_01170 0.90 -1.7 252,679 - rfbA OKGIIK_01170 0.90 -1.5 252,679 - rfbA OKGIIK_01170 0.90 -1.7 252,679 - rfbA OKGIIK_01170 0.90 -2.3 252,791 + -1.3 252,791 + -0.5 252,792 - -0.2 252,792 - -1.7 252,792 - +0.0 252,792 - -0.3 252,836 + -3.0 252,836 + -2.4 252,836 + +0.2 252,836 + -3.5 252,836 + +1.1 252,837 - -2.5 252,837 - -1.3 252,837 - -2.6 252,837 - +0.1 252,837 - +0.7 252,837 - -1.7 252,837 - -1.9 252,837 - -0.9
Or see this region's nucleotide sequence