Experiment: R2A_PIPES with Metal_ion_mix_sitestressplate 2 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tsaA and OKGIIK_00410 are separated by 58 nucleotides OKGIIK_00410 and OKGIIK_00415 are separated by 2 nucleotides OKGIIK_00415 and OKGIIK_00420 are separated by 28 nucleotides OKGIIK_00420 and OKGIIK_00425 are separated by 160 nucleotides
OKGIIK_00405: tsaA - tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO, at 98,029 to 98,604
tsaA
OKGIIK_00410: OKGIIK_00410 - YCII domain-containing protein, at 98,663 to 99,037
_00410
OKGIIK_00415: OKGIIK_00415 - hypothetical protein, at 99,040 to 99,561
_00415
OKGIIK_00420: OKGIIK_00420 - haloacid dehalogenase, at 99,590 to 100,306
_00420
OKGIIK_00425: OKGIIK_00425 - hypothetical protein, at 100,467 to 100,721
_00425
Position (kb) 99
100 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 98.162 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.172 kb on - strand, within tsaA at 98.172 kb on - strand, within tsaA at 98.172 kb on - strand, within tsaA at 98.172 kb on - strand, within tsaA at 98.172 kb on - strand, within tsaA at 98.172 kb on - strand, within tsaA at 98.172 kb on - strand, within tsaA at 98.172 kb on - strand, within tsaA at 98.528 kb on + strand, within tsaA at 98.528 kb on + strand, within tsaA at 98.528 kb on + strand, within tsaA at 98.529 kb on - strand, within tsaA at 98.577 kb on + strand at 98.577 kb on + strand at 98.577 kb on + strand at 98.577 kb on + strand at 98.577 kb on + strand at 98.577 kb on + strand at 98.578 kb on - strand at 98.578 kb on - strand at 98.578 kb on - strand at 98.578 kb on - strand at 98.578 kb on - strand at 98.578 kb on - strand at 98.578 kb on - strand at 98.606 kb on - strand at 98.606 kb on - strand at 98.684 kb on + strand at 98.684 kb on + strand at 98.684 kb on + strand at 98.684 kb on + strand at 98.684 kb on + strand at 98.684 kb on + strand at 98.684 kb on + strand at 98.684 kb on + strand at 98.685 kb on - strand at 98.685 kb on - strand at 98.685 kb on - strand at 98.685 kb on - strand at 98.685 kb on - strand at 98.685 kb on - strand at 98.685 kb on - strand at 98.685 kb on - strand at 98.891 kb on + strand, within OKGIIK_00410 at 98.891 kb on + strand, within OKGIIK_00410 at 98.891 kb on + strand, within OKGIIK_00410 at 98.891 kb on + strand, within OKGIIK_00410 at 98.891 kb on + strand, within OKGIIK_00410 at 98.891 kb on + strand, within OKGIIK_00410 at 98.891 kb on + strand, within OKGIIK_00410 at 98.892 kb on - strand, within OKGIIK_00410 at 98.892 kb on - strand, within OKGIIK_00410 at 98.892 kb on - strand, within OKGIIK_00410 at 98.892 kb on - strand, within OKGIIK_00410 at 98.892 kb on - strand, within OKGIIK_00410 at 99.404 kb on - strand, within OKGIIK_00415 at 99.404 kb on - strand, within OKGIIK_00415 at 99.427 kb on + strand, within OKGIIK_00415 at 99.428 kb on - strand, within OKGIIK_00415 at 99.644 kb on + strand at 99.644 kb on + strand at 99.644 kb on + strand at 99.645 kb on - strand at 99.645 kb on - strand at 99.645 kb on - strand at 99.645 kb on - strand at 99.647 kb on + strand at 99.689 kb on + strand, within OKGIIK_00420 at 99.689 kb on + strand, within OKGIIK_00420 at 99.689 kb on + strand, within OKGIIK_00420 at 99.689 kb on + strand, within OKGIIK_00420 at 99.690 kb on - strand, within OKGIIK_00420 at 99.690 kb on - strand, within OKGIIK_00420 at 99.690 kb on - strand, within OKGIIK_00420 at 99.938 kb on + strand, within OKGIIK_00420 at 99.938 kb on + strand, within OKGIIK_00420 at 99.938 kb on + strand, within OKGIIK_00420 at 99.938 kb on + strand, within OKGIIK_00420 at 99.938 kb on + strand, within OKGIIK_00420 at 99.938 kb on + strand, within OKGIIK_00420 at 99.938 kb on + strand, within OKGIIK_00420 at 99.938 kb on + strand, within OKGIIK_00420 at 99.938 kb on + strand, within OKGIIK_00420 at 99.939 kb on - strand, within OKGIIK_00420 at 99.939 kb on - strand, within OKGIIK_00420 at 99.939 kb on - strand, within OKGIIK_00420 at 99.939 kb on - strand, within OKGIIK_00420 at 99.939 kb on - strand, within OKGIIK_00420 at 99.939 kb on - strand, within OKGIIK_00420 at 99.939 kb on - strand, within OKGIIK_00420 at 99.939 kb on - strand, within OKGIIK_00420 at 99.939 kb on - strand, within OKGIIK_00420 at 99.939 kb on - strand, within OKGIIK_00420 at 100.058 kb on + strand, within OKGIIK_00420 at 100.058 kb on + strand, within OKGIIK_00420 at 100.059 kb on - strand, within OKGIIK_00420 at 100.178 kb on + strand, within OKGIIK_00420 at 100.179 kb on - strand, within OKGIIK_00420 at 100.179 kb on - strand, within OKGIIK_00420 at 100.304 kb on + strand at 100.304 kb on + strand at 100.305 kb on - strand at 100.305 kb on - strand at 100.319 kb on + strand at 100.319 kb on + strand at 100.319 kb on + strand at 100.320 kb on - strand at 100.320 kb on - strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.453 kb on + strand at 100.468 kb on + strand at 100.468 kb on + strand at 100.468 kb on + strand at 100.468 kb on + strand at 100.468 kb on + strand at 100.468 kb on + strand at 100.468 kb on + strand at 100.468 kb on + strand at 100.468 kb on + strand at 100.468 kb on + strand at 100.468 kb on + strand at 100.468 kb on + strand at 100.469 kb on - strand at 100.469 kb on - strand at 100.469 kb on - strand at 100.469 kb on - strand at 100.486 kb on + strand at 100.486 kb on + strand at 100.486 kb on + strand at 100.486 kb on + strand at 100.486 kb on + strand at 100.486 kb on + strand at 100.487 kb on - strand at 100.487 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Metal_ion_mix_sitestressplate 2 x remove 98,162 + tsaA OKGIIK_00405 0.23 +0.7 98,171 + tsaA OKGIIK_00405 0.25 +0.4 98,171 + tsaA OKGIIK_00405 0.25 -1.3 98,171 + tsaA OKGIIK_00405 0.25 -0.1 98,171 + tsaA OKGIIK_00405 0.25 +0.1 98,171 + tsaA OKGIIK_00405 0.25 +1.1 98,171 + tsaA OKGIIK_00405 0.25 -0.9 98,171 + tsaA OKGIIK_00405 0.25 -0.9 98,171 + tsaA OKGIIK_00405 0.25 -1.3 98,171 + tsaA OKGIIK_00405 0.25 -2.6 98,171 + tsaA OKGIIK_00405 0.25 -0.0 98,171 + tsaA OKGIIK_00405 0.25 +1.7 98,171 + tsaA OKGIIK_00405 0.25 -2.4 98,171 + tsaA OKGIIK_00405 0.25 -0.9 98,171 + tsaA OKGIIK_00405 0.25 -0.9 98,172 - tsaA OKGIIK_00405 0.25 -2.4 98,172 - tsaA OKGIIK_00405 0.25 -1.1 98,172 - tsaA OKGIIK_00405 0.25 -0.4 98,172 - tsaA OKGIIK_00405 0.25 -1.9 98,172 - tsaA OKGIIK_00405 0.25 -0.4 98,172 - tsaA OKGIIK_00405 0.25 +2.7 98,172 - tsaA OKGIIK_00405 0.25 -0.1 98,172 - tsaA OKGIIK_00405 0.25 -0.5 98,528 + tsaA OKGIIK_00405 0.87 +0.1 98,528 + tsaA OKGIIK_00405 0.87 -0.4 98,528 + tsaA OKGIIK_00405 0.87 +0.1 98,529 - tsaA OKGIIK_00405 0.87 -0.3 98,577 + +1.1 98,577 + +0.1 98,577 + +0.7 98,577 + -0.3 98,577 + +1.3 98,577 + -0.3 98,578 - -0.4 98,578 - +0.1 98,578 - -1.3 98,578 - -2.7 98,578 - -0.3 98,578 - -0.9 98,578 - -2.3 98,606 - +0.9 98,606 - +0.8 98,684 + -0.5 98,684 + -0.3 98,684 + -0.9 98,684 + +0.7 98,684 + +1.1 98,684 + -0.7 98,684 + +0.2 98,684 + -0.5 98,685 - +1.3 98,685 - -0.9 98,685 - +0.5 98,685 - -1.3 98,685 - -0.3 98,685 - -0.3 98,685 - -0.6 98,685 - -2.4 98,891 + OKGIIK_00410 0.61 -0.9 98,891 + OKGIIK_00410 0.61 +0.8 98,891 + OKGIIK_00410 0.61 -1.0 98,891 + OKGIIK_00410 0.61 -1.9 98,891 + OKGIIK_00410 0.61 +1.1 98,891 + OKGIIK_00410 0.61 +0.9 98,891 + OKGIIK_00410 0.61 -1.1 98,892 - OKGIIK_00410 0.61 +0.6 98,892 - OKGIIK_00410 0.61 +0.2 98,892 - OKGIIK_00410 0.61 -0.6 98,892 - OKGIIK_00410 0.61 +0.2 98,892 - OKGIIK_00410 0.61 -1.5 99,404 - OKGIIK_00415 0.70 -1.2 99,404 - OKGIIK_00415 0.70 -3.3 99,427 + OKGIIK_00415 0.74 -1.9 99,428 - OKGIIK_00415 0.74 -2.4 99,644 + -0.9 99,644 + +2.3 99,644 + -0.6 99,645 - -1.1 99,645 - -2.3 99,645 - -2.0 99,645 - -1.7 99,647 + -1.5 99,689 + OKGIIK_00420 0.14 -1.7 99,689 + OKGIIK_00420 0.14 +0.1 99,689 + OKGIIK_00420 0.14 -0.5 99,689 + OKGIIK_00420 0.14 +1.1 99,690 - OKGIIK_00420 0.14 +2.1 99,690 - OKGIIK_00420 0.14 -1.5 99,690 - OKGIIK_00420 0.14 +0.5 99,938 + OKGIIK_00420 0.49 +0.3 99,938 + OKGIIK_00420 0.49 -1.3 99,938 + OKGIIK_00420 0.49 -0.9 99,938 + OKGIIK_00420 0.49 +0.4 99,938 + OKGIIK_00420 0.49 +0.3 99,938 + OKGIIK_00420 0.49 -0.5 99,938 + OKGIIK_00420 0.49 -2.3 99,938 + OKGIIK_00420 0.49 -0.7 99,938 + OKGIIK_00420 0.49 -1.5 99,939 - OKGIIK_00420 0.49 -0.9 99,939 - OKGIIK_00420 0.49 -2.5 99,939 - OKGIIK_00420 0.49 +0.7 99,939 - OKGIIK_00420 0.49 -1.3 99,939 - OKGIIK_00420 0.49 +1.1 99,939 - OKGIIK_00420 0.49 -0.1 99,939 - OKGIIK_00420 0.49 +0.1 99,939 - OKGIIK_00420 0.49 -0.5 99,939 - OKGIIK_00420 0.49 +0.7 99,939 - OKGIIK_00420 0.49 -0.3 100,058 + OKGIIK_00420 0.65 -0.8 100,058 + OKGIIK_00420 0.65 -1.3 100,059 - OKGIIK_00420 0.65 -1.1 100,178 + OKGIIK_00420 0.82 +0.5 100,179 - OKGIIK_00420 0.82 -0.7 100,179 - OKGIIK_00420 0.82 +0.5 100,304 + -0.4 100,304 + -0.9 100,305 - +1.5 100,305 - +0.4 100,319 + -0.7 100,319 + -1.9 100,319 + -0.2 100,320 - -0.9 100,320 - -0.1 100,381 + -3.1 100,381 + -1.3 100,381 + -2.4 100,381 + -3.2 100,381 + -0.9 100,381 + -0.9 100,381 + -1.6 100,381 + -2.3 100,381 + +0.4 100,381 + -1.6 100,381 + +1.7 100,381 + +0.1 100,381 + -0.4 100,381 + -1.7 100,381 + +0.1 100,382 - -0.9 100,382 - -0.2 100,382 - -0.5 100,382 - -1.9 100,382 - -2.9 100,382 - +2.1 100,382 - +0.5 100,382 - -0.1 100,382 - +0.3 100,382 - +0.1 100,382 - +1.1 100,382 - +0.3 100,382 - -0.1 100,382 - -1.5 100,382 - +0.8 100,382 - -1.0 100,382 - -1.3 100,453 + -0.4 100,468 + +0.5 100,468 + -1.5 100,468 + -1.4 100,468 + -1.0 100,468 + +2.1 100,468 + -0.7 100,468 + -0.5 100,468 + +1.1 100,468 + -2.4 100,468 + -2.4 100,468 + -0.9 100,468 + -0.7 100,469 - -1.0 100,469 - -0.5 100,469 - +1.1 100,469 - +1.1 100,486 + -2.4 100,486 + -1.6 100,486 + +0.7 100,486 + -0.9 100,486 + +0.1 100,486 + -1.7 100,487 - -2.5 100,487 - -3.6
Or see this region's nucleotide sequence