Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_00715 and ygjP are separated by 59 nucleotides ygjP and OKGIIK_00725 are separated by 2 nucleotides OKGIIK_00725 and OKGIIK_00730 are separated by 583 nucleotides
OKGIIK_00715: OKGIIK_00715 - glyoxalase, at 157,189 to 157,572
_00715
OKGIIK_00720: ygjP - metal-dependent hydrolase, at 157,632 to 158,150
ygjP
OKGIIK_00725: OKGIIK_00725 - DUF6348 domain-containing protein, at 158,153 to 158,797
_00725
OKGIIK_00730: OKGIIK_00730 - Peptide transporter, at 159,381 to 161,048
_00730
Position (kb) 158
159 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 157.442 kb on + strand, within OKGIIK_00715 at 157.442 kb on + strand, within OKGIIK_00715 at 157.443 kb on - strand, within OKGIIK_00715 at 157.443 kb on - strand, within OKGIIK_00715 at 157.443 kb on - strand, within OKGIIK_00715 at 157.443 kb on - strand, within OKGIIK_00715 at 157.443 kb on - strand, within OKGIIK_00715 at 157.443 kb on - strand, within OKGIIK_00715 at 157.597 kb on + strand at 157.597 kb on + strand at 157.597 kb on + strand at 157.597 kb on + strand at 157.598 kb on - strand at 157.598 kb on - strand at 157.616 kb on + strand at 157.617 kb on - strand at 157.624 kb on + strand at 157.624 kb on + strand at 157.625 kb on - strand at 157.625 kb on - strand at 157.647 kb on + strand at 157.647 kb on + strand at 157.647 kb on + strand at 157.647 kb on + strand at 157.648 kb on - strand at 157.659 kb on + strand at 157.773 kb on + strand, within ygjP at 157.773 kb on + strand, within ygjP at 157.773 kb on + strand, within ygjP at 157.773 kb on + strand, within ygjP at 157.774 kb on - strand, within ygjP at 157.774 kb on - strand, within ygjP at 157.774 kb on - strand, within ygjP at 157.779 kb on + strand, within ygjP at 157.779 kb on + strand, within ygjP at 157.779 kb on + strand, within ygjP at 157.780 kb on - strand, within ygjP at 157.780 kb on - strand, within ygjP at 157.780 kb on - strand, within ygjP at 157.780 kb on - strand, within ygjP at 157.780 kb on - strand, within ygjP at 157.839 kb on + strand, within ygjP at 157.839 kb on + strand, within ygjP at 157.839 kb on + strand, within ygjP at 157.839 kb on + strand, within ygjP at 157.840 kb on - strand, within ygjP at 157.840 kb on - strand, within ygjP at 157.840 kb on - strand, within ygjP at 157.840 kb on - strand, within ygjP at 158.037 kb on + strand, within ygjP at 158.037 kb on + strand, within ygjP at 158.037 kb on + strand, within ygjP at 158.037 kb on + strand, within ygjP at 158.037 kb on + strand, within ygjP at 158.037 kb on + strand, within ygjP at 158.037 kb on + strand, within ygjP at 158.037 kb on + strand, within ygjP at 158.052 kb on + strand, within ygjP at 158.052 kb on + strand, within ygjP at 158.052 kb on + strand, within ygjP at 158.052 kb on + strand, within ygjP at 158.052 kb on + strand, within ygjP at 158.052 kb on + strand, within ygjP at 158.052 kb on + strand, within ygjP at 158.052 kb on + strand, within ygjP at 158.052 kb on + strand, within ygjP at 158.052 kb on + strand, within ygjP at 158.052 kb on + strand, within ygjP at 158.053 kb on - strand, within ygjP at 158.053 kb on - strand, within ygjP at 158.053 kb on - strand, within ygjP at 158.053 kb on - strand, within ygjP at 158.053 kb on - strand, within ygjP at 158.053 kb on - strand, within ygjP at 158.053 kb on - strand, within ygjP at 158.053 kb on - strand, within ygjP at 158.067 kb on + strand, within ygjP at 158.067 kb on + strand, within ygjP at 158.068 kb on - strand, within ygjP at 158.097 kb on + strand, within ygjP at 158.097 kb on + strand, within ygjP at 158.097 kb on + strand, within ygjP at 158.098 kb on - strand, within ygjP at 158.098 kb on - strand, within ygjP at 158.177 kb on + strand at 158.177 kb on + strand at 158.177 kb on + strand at 158.177 kb on + strand at 158.177 kb on + strand at 158.177 kb on + strand at 158.178 kb on - strand at 158.178 kb on - strand at 158.178 kb on - strand at 158.178 kb on - strand at 158.450 kb on + strand, within OKGIIK_00725 at 158.450 kb on + strand, within OKGIIK_00725 at 158.450 kb on + strand, within OKGIIK_00725 at 158.450 kb on + strand, within OKGIIK_00725 at 158.450 kb on + strand, within OKGIIK_00725 at 158.450 kb on + strand, within OKGIIK_00725 at 158.450 kb on + strand, within OKGIIK_00725 at 158.451 kb on - strand, within OKGIIK_00725 at 158.451 kb on - strand, within OKGIIK_00725 at 158.451 kb on - strand, within OKGIIK_00725 at 158.753 kb on + strand at 158.753 kb on + strand at 158.753 kb on + strand at 158.753 kb on + strand at 158.754 kb on - strand at 158.754 kb on - strand at 158.792 kb on + strand at 158.985 kb on - strand at 158.985 kb on - strand at 158.985 kb on - strand at 159.013 kb on + strand at 159.013 kb on + strand at 159.013 kb on + strand at 159.013 kb on + strand at 159.014 kb on - strand at 159.014 kb on - strand at 159.014 kb on - strand at 159.014 kb on - strand at 159.014 kb on - strand at 159.036 kb on + strand at 159.119 kb on + strand at 159.119 kb on + strand at 159.222 kb on + strand at 159.222 kb on + strand at 159.222 kb on + strand at 159.222 kb on + strand at 159.223 kb on - strand at 159.223 kb on - strand at 159.223 kb on - strand at 159.223 kb on - strand at 159.223 kb on - strand at 159.223 kb on - strand at 159.223 kb on - strand at 159.223 kb on - strand at 159.223 kb on - strand at 159.223 kb on - strand at 159.223 kb on - strand at 159.317 kb on + strand at 159.317 kb on + strand at 159.317 kb on + strand at 159.318 kb on - strand at 159.318 kb on - strand at 159.318 kb on - strand at 159.318 kb on - strand at 159.346 kb on + strand at 159.346 kb on + strand at 159.346 kb on + strand at 159.346 kb on + strand at 159.346 kb on + strand at 159.347 kb on - strand at 159.347 kb on - strand at 159.347 kb on - strand at 159.347 kb on - strand at 159.347 kb on - strand at 159.347 kb on - strand at 159.347 kb on - strand at 159.347 kb on - strand at 159.347 kb on - strand at 159.348 kb on + strand at 159.348 kb on + strand at 159.348 kb on + strand at 159.348 kb on + strand at 159.348 kb on + strand at 159.348 kb on + strand at 159.348 kb on + strand at 159.349 kb on - strand at 159.349 kb on - strand at 159.349 kb on - strand at 159.349 kb on - strand at 159.349 kb on - strand at 159.349 kb on - strand at 159.349 kb on - strand at 159.349 kb on - strand at 159.349 kb on - strand at 159.349 kb on - strand at 159.352 kb on + strand at 159.352 kb on + strand at 159.352 kb on + strand at 159.353 kb on - strand at 159.353 kb on - strand at 159.353 kb on - strand at 159.353 kb on - strand at 159.353 kb on - strand at 159.353 kb on - strand at 159.353 kb on - strand at 159.353 kb on - strand at 159.398 kb on - strand at 159.398 kb on - strand at 159.409 kb on + strand at 159.409 kb on + strand at 159.409 kb on + strand at 159.409 kb on + strand at 159.410 kb on - strand at 159.410 kb on - strand at 159.410 kb on - strand at 159.486 kb on - strand at 159.674 kb on + strand, within OKGIIK_00730 at 159.674 kb on + strand, within OKGIIK_00730 at 159.674 kb on + strand, within OKGIIK_00730 at 159.675 kb on - strand, within OKGIIK_00730 at 159.708 kb on + strand, within OKGIIK_00730 at 159.708 kb on + strand, within OKGIIK_00730 at 159.709 kb on - strand, within OKGIIK_00730 at 159.709 kb on - strand, within OKGIIK_00730 at 159.723 kb on + strand, within OKGIIK_00730 at 159.723 kb on + strand, within OKGIIK_00730 at 159.723 kb on + strand, within OKGIIK_00730 at 159.723 kb on + strand, within OKGIIK_00730 at 159.724 kb on - strand, within OKGIIK_00730 at 159.724 kb on - strand, within OKGIIK_00730 at 159.741 kb on + strand, within OKGIIK_00730 at 159.741 kb on + strand, within OKGIIK_00730 at 159.741 kb on + strand, within OKGIIK_00730 at 159.742 kb on - strand, within OKGIIK_00730 at 159.742 kb on - strand, within OKGIIK_00730 at 159.742 kb on - strand, within OKGIIK_00730
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 157,442 + OKGIIK_00715 0.66 -0.4 157,442 + OKGIIK_00715 0.66 -3.3 157,443 - OKGIIK_00715 0.66 +0.4 157,443 - OKGIIK_00715 0.66 +0.9 157,443 - OKGIIK_00715 0.66 -1.7 157,443 - OKGIIK_00715 0.66 +0.6 157,443 - OKGIIK_00715 0.66 +1.6 157,443 - OKGIIK_00715 0.66 -2.2 157,597 + -3.7 157,597 + -2.2 157,597 + -1.2 157,597 + -0.3 157,598 - +0.0 157,598 - -1.4 157,616 + -0.4 157,617 - -0.4 157,624 + -1.6 157,624 + -2.6 157,625 - -1.2 157,625 - +0.9 157,647 + -2.2 157,647 + -1.0 157,647 + -0.4 157,647 + +0.6 157,648 - -1.4 157,659 + -0.7 157,773 + ygjP OKGIIK_00720 0.27 -1.2 157,773 + ygjP OKGIIK_00720 0.27 -1.4 157,773 + ygjP OKGIIK_00720 0.27 -1.9 157,773 + ygjP OKGIIK_00720 0.27 -1.4 157,774 - ygjP OKGIIK_00720 0.27 +3.2 157,774 - ygjP OKGIIK_00720 0.27 +0.6 157,774 - ygjP OKGIIK_00720 0.27 +0.6 157,779 + ygjP OKGIIK_00720 0.28 -2.9 157,779 + ygjP OKGIIK_00720 0.28 +0.0 157,779 + ygjP OKGIIK_00720 0.28 -1.0 157,780 - ygjP OKGIIK_00720 0.29 -1.0 157,780 - ygjP OKGIIK_00720 0.29 +1.6 157,780 - ygjP OKGIIK_00720 0.29 -1.2 157,780 - ygjP OKGIIK_00720 0.29 +0.9 157,780 - ygjP OKGIIK_00720 0.29 -0.4 157,839 + ygjP OKGIIK_00720 0.40 -2.1 157,839 + ygjP OKGIIK_00720 0.40 -2.4 157,839 + ygjP OKGIIK_00720 0.40 +0.0 157,839 + ygjP OKGIIK_00720 0.40 -2.7 157,840 - ygjP OKGIIK_00720 0.40 +1.0 157,840 - ygjP OKGIIK_00720 0.40 +0.2 157,840 - ygjP OKGIIK_00720 0.40 +0.0 157,840 - ygjP OKGIIK_00720 0.40 -1.0 158,037 + ygjP OKGIIK_00720 0.78 -0.7 158,037 + ygjP OKGIIK_00720 0.78 -1.2 158,037 + ygjP OKGIIK_00720 0.78 -0.4 158,037 + ygjP OKGIIK_00720 0.78 -2.8 158,037 + ygjP OKGIIK_00720 0.78 -2.6 158,037 + ygjP OKGIIK_00720 0.78 +1.6 158,037 + ygjP OKGIIK_00720 0.78 +0.9 158,037 + ygjP OKGIIK_00720 0.78 +0.6 158,052 + ygjP OKGIIK_00720 0.81 +1.6 158,052 + ygjP OKGIIK_00720 0.81 -0.9 158,052 + ygjP OKGIIK_00720 0.81 +1.6 158,052 + ygjP OKGIIK_00720 0.81 -0.3 158,052 + ygjP OKGIIK_00720 0.81 +1.1 158,052 + ygjP OKGIIK_00720 0.81 -2.4 158,052 + ygjP OKGIIK_00720 0.81 +1.2 158,052 + ygjP OKGIIK_00720 0.81 -0.3 158,052 + ygjP OKGIIK_00720 0.81 -3.5 158,052 + ygjP OKGIIK_00720 0.81 -2.2 158,052 + ygjP OKGIIK_00720 0.81 -1.9 158,053 - ygjP OKGIIK_00720 0.81 +0.6 158,053 - ygjP OKGIIK_00720 0.81 -1.0 158,053 - ygjP OKGIIK_00720 0.81 -1.4 158,053 - ygjP OKGIIK_00720 0.81 -1.9 158,053 - ygjP OKGIIK_00720 0.81 -2.9 158,053 - ygjP OKGIIK_00720 0.81 +0.1 158,053 - ygjP OKGIIK_00720 0.81 -2.1 158,053 - ygjP OKGIIK_00720 0.81 +0.1 158,067 + ygjP OKGIIK_00720 0.84 -1.2 158,067 + ygjP OKGIIK_00720 0.84 -0.4 158,068 - ygjP OKGIIK_00720 0.84 +1.2 158,097 + ygjP OKGIIK_00720 0.90 +0.0 158,097 + ygjP OKGIIK_00720 0.90 -1.1 158,097 + ygjP OKGIIK_00720 0.90 -0.7 158,098 - ygjP OKGIIK_00720 0.90 -2.3 158,098 - ygjP OKGIIK_00720 0.90 -0.4 158,177 + -1.9 158,177 + -1.2 158,177 + -1.4 158,177 + -0.7 158,177 + -0.7 158,177 + -1.0 158,178 - -0.6 158,178 - -1.3 158,178 - -1.4 158,178 - -0.4 158,450 + OKGIIK_00725 0.46 -2.7 158,450 + OKGIIK_00725 0.46 +0.0 158,450 + OKGIIK_00725 0.46 -2.2 158,450 + OKGIIK_00725 0.46 -2.9 158,450 + OKGIIK_00725 0.46 -0.4 158,450 + OKGIIK_00725 0.46 -3.1 158,450 + OKGIIK_00725 0.46 -1.2 158,451 - OKGIIK_00725 0.46 +0.2 158,451 - OKGIIK_00725 0.46 -1.0 158,451 - OKGIIK_00725 0.46 -1.9 158,753 + +0.6 158,753 + +2.4 158,753 + -0.4 158,753 + -2.0 158,754 - -0.2 158,754 - -1.0 158,792 + -1.2 158,985 - -1.7 158,985 - -1.0 158,985 - -1.4 159,013 + +1.6 159,013 + -2.5 159,013 + -1.4 159,013 + -0.4 159,014 - -1.0 159,014 - -4.0 159,014 - -1.4 159,014 - -2.3 159,014 - -1.9 159,036 + -2.0 159,119 + -0.7 159,119 + -2.2 159,222 + +0.8 159,222 + -2.5 159,222 + -1.2 159,222 + +0.0 159,223 - -3.9 159,223 - -1.9 159,223 - -1.6 159,223 - -1.6 159,223 - -1.0 159,223 - -2.0 159,223 - -2.1 159,223 - +1.6 159,223 - -2.5 159,223 - +0.6 159,223 - +1.6 159,317 + +0.6 159,317 + -1.2 159,317 + +1.0 159,318 - -2.3 159,318 - -3.8 159,318 - -2.2 159,318 - -1.6 159,346 + -0.4 159,346 + +1.6 159,346 + +0.5 159,346 + -1.0 159,346 + -1.9 159,347 - +1.0 159,347 - -2.4 159,347 - -1.2 159,347 - -3.1 159,347 - -3.2 159,347 - -0.4 159,347 - -2.8 159,347 - +0.5 159,347 - -2.1 159,348 + -1.8 159,348 + -1.2 159,348 + -4.0 159,348 + -1.2 159,348 + -2.9 159,348 + -0.4 159,348 + -2.1 159,349 - -0.4 159,349 - -2.9 159,349 - -0.4 159,349 - -1.9 159,349 - +0.6 159,349 - +2.4 159,349 - +0.7 159,349 - -2.8 159,349 - +1.0 159,349 - -2.0 159,352 + -0.4 159,352 + -1.0 159,352 + +0.3 159,353 - -0.4 159,353 - +0.9 159,353 - -2.6 159,353 - -0.1 159,353 - +1.6 159,353 - -0.4 159,353 - -1.7 159,353 - +0.5 159,398 - +0.9 159,398 - +0.6 159,409 + +1.1 159,409 + +1.6 159,409 + -1.0 159,409 + +1.0 159,410 - -1.0 159,410 - -2.0 159,410 - +3.5 159,486 - -2.1 159,674 + OKGIIK_00730 0.18 +1.6 159,674 + OKGIIK_00730 0.18 -2.2 159,674 + OKGIIK_00730 0.18 -1.2 159,675 - OKGIIK_00730 0.18 -0.7 159,708 + OKGIIK_00730 0.20 -2.4 159,708 + OKGIIK_00730 0.20 +0.0 159,709 - OKGIIK_00730 0.20 -0.4 159,709 - OKGIIK_00730 0.20 +0.5 159,723 + OKGIIK_00730 0.21 -1.0 159,723 + OKGIIK_00730 0.21 -1.7 159,723 + OKGIIK_00730 0.21 +1.0 159,723 + OKGIIK_00730 0.21 +0.0 159,724 - OKGIIK_00730 0.21 +0.6 159,724 - OKGIIK_00730 0.21 -2.4 159,741 + OKGIIK_00730 0.22 -4.6 159,741 + OKGIIK_00730 0.22 -1.6 159,741 + OKGIIK_00730 0.22 -2.2 159,742 - OKGIIK_00730 0.22 -1.4 159,742 - OKGIIK_00730 0.22 -2.8 159,742 - OKGIIK_00730 0.22 +1.2
Or see this region's nucleotide sequence