Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_00715 and ygjP are separated by 59 nucleotides ygjP and OKGIIK_00725 are separated by 2 nucleotides OKGIIK_00725 and OKGIIK_00730 are separated by 583 nucleotides
OKGIIK_00715: OKGIIK_00715 - glyoxalase, at 157,189 to 157,572
_00715
OKGIIK_00720: ygjP - metal-dependent hydrolase, at 157,632 to 158,150
ygjP
OKGIIK_00725: OKGIIK_00725 - DUF6348 domain-containing protein, at 158,153 to 158,797
_00725
OKGIIK_00730: OKGIIK_00730 - Peptide transporter, at 159,381 to 161,048
_00730
Position (kb) 158
159 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 157.442 kb on + strand, within OKGIIK_00715 at 157.442 kb on + strand, within OKGIIK_00715 at 157.443 kb on - strand, within OKGIIK_00715 at 157.443 kb on - strand, within OKGIIK_00715 at 157.443 kb on - strand, within OKGIIK_00715 at 157.443 kb on - strand, within OKGIIK_00715 at 157.443 kb on - strand, within OKGIIK_00715 at 157.443 kb on - strand, within OKGIIK_00715 at 157.597 kb on + strand at 157.597 kb on + strand at 157.597 kb on + strand at 157.597 kb on + strand at 157.598 kb on - strand at 157.598 kb on - strand at 157.616 kb on + strand at 157.617 kb on - strand at 157.624 kb on + strand at 157.624 kb on + strand at 157.625 kb on - strand at 157.625 kb on - strand at 157.647 kb on + strand at 157.647 kb on + strand at 157.647 kb on + strand at 157.647 kb on + strand at 157.648 kb on - strand at 157.659 kb on + strand at 157.773 kb on + strand, within ygjP at 157.773 kb on + strand, within ygjP at 157.773 kb on + strand, within ygjP at 157.773 kb on + strand, within ygjP at 157.774 kb on - strand, within ygjP at 157.774 kb on - strand, within ygjP at 157.774 kb on - strand, within ygjP at 157.779 kb on + strand, within ygjP at 157.779 kb on + strand, within ygjP at 157.779 kb on + strand, within ygjP at 157.780 kb on - strand, within ygjP at 157.780 kb on - strand, within ygjP at 157.780 kb on - strand, within ygjP at 157.780 kb on - strand, within ygjP at 157.780 kb on - strand, within ygjP at 157.839 kb on + strand, within ygjP at 157.839 kb on + strand, within ygjP at 157.839 kb on + strand, within ygjP at 157.839 kb on + strand, within ygjP at 157.840 kb on - strand, within ygjP at 157.840 kb on - strand, within ygjP at 157.840 kb on - strand, within ygjP at 157.840 kb on - strand, within ygjP at 158.037 kb on + strand, within ygjP at 158.037 kb on + strand, within ygjP at 158.037 kb on + strand, within ygjP at 158.037 kb on + strand, within ygjP at 158.037 kb on + strand, within ygjP at 158.037 kb on + strand, within ygjP at 158.037 kb on + strand, within ygjP at 158.037 kb on + strand, within ygjP at 158.052 kb on + strand, within ygjP at 158.052 kb on + strand, within ygjP at 158.052 kb on + strand, within ygjP at 158.052 kb on + strand, within ygjP at 158.052 kb on + strand, within ygjP at 158.052 kb on + strand, within ygjP at 158.052 kb on + strand, within ygjP at 158.052 kb on + strand, within ygjP at 158.052 kb on + strand, within ygjP at 158.052 kb on + strand, within ygjP at 158.052 kb on + strand, within ygjP at 158.053 kb on - strand, within ygjP at 158.053 kb on - strand, within ygjP at 158.053 kb on - strand, within ygjP at 158.053 kb on - strand, within ygjP at 158.053 kb on - strand, within ygjP at 158.053 kb on - strand, within ygjP at 158.053 kb on - strand, within ygjP at 158.053 kb on - strand, within ygjP at 158.067 kb on + strand, within ygjP at 158.067 kb on + strand, within ygjP at 158.068 kb on - strand, within ygjP at 158.097 kb on + strand, within ygjP at 158.097 kb on + strand, within ygjP at 158.097 kb on + strand, within ygjP at 158.098 kb on - strand, within ygjP at 158.098 kb on - strand, within ygjP at 158.177 kb on + strand at 158.177 kb on + strand at 158.177 kb on + strand at 158.177 kb on + strand at 158.177 kb on + strand at 158.177 kb on + strand at 158.178 kb on - strand at 158.178 kb on - strand at 158.178 kb on - strand at 158.178 kb on - strand at 158.450 kb on + strand, within OKGIIK_00725 at 158.450 kb on + strand, within OKGIIK_00725 at 158.450 kb on + strand, within OKGIIK_00725 at 158.450 kb on + strand, within OKGIIK_00725 at 158.450 kb on + strand, within OKGIIK_00725 at 158.450 kb on + strand, within OKGIIK_00725 at 158.450 kb on + strand, within OKGIIK_00725 at 158.451 kb on - strand, within OKGIIK_00725 at 158.451 kb on - strand, within OKGIIK_00725 at 158.451 kb on - strand, within OKGIIK_00725 at 158.753 kb on + strand at 158.753 kb on + strand at 158.753 kb on + strand at 158.753 kb on + strand at 158.754 kb on - strand at 158.754 kb on - strand at 158.792 kb on + strand at 158.985 kb on - strand at 158.985 kb on - strand at 158.985 kb on - strand at 159.013 kb on + strand at 159.013 kb on + strand at 159.013 kb on + strand at 159.013 kb on + strand at 159.014 kb on - strand at 159.014 kb on - strand at 159.014 kb on - strand at 159.014 kb on - strand at 159.014 kb on - strand at 159.036 kb on + strand at 159.119 kb on + strand at 159.119 kb on + strand at 159.222 kb on + strand at 159.222 kb on + strand at 159.222 kb on + strand at 159.222 kb on + strand at 159.223 kb on - strand at 159.223 kb on - strand at 159.223 kb on - strand at 159.223 kb on - strand at 159.223 kb on - strand at 159.223 kb on - strand at 159.223 kb on - strand at 159.223 kb on - strand at 159.223 kb on - strand at 159.223 kb on - strand at 159.223 kb on - strand at 159.317 kb on + strand at 159.317 kb on + strand at 159.317 kb on + strand at 159.318 kb on - strand at 159.318 kb on - strand at 159.318 kb on - strand at 159.318 kb on - strand at 159.346 kb on + strand at 159.346 kb on + strand at 159.346 kb on + strand at 159.346 kb on + strand at 159.346 kb on + strand at 159.347 kb on - strand at 159.347 kb on - strand at 159.347 kb on - strand at 159.347 kb on - strand at 159.347 kb on - strand at 159.347 kb on - strand at 159.347 kb on - strand at 159.347 kb on - strand at 159.347 kb on - strand at 159.348 kb on + strand at 159.348 kb on + strand at 159.348 kb on + strand at 159.348 kb on + strand at 159.348 kb on + strand at 159.348 kb on + strand at 159.348 kb on + strand at 159.349 kb on - strand at 159.349 kb on - strand at 159.349 kb on - strand at 159.349 kb on - strand at 159.349 kb on - strand at 159.349 kb on - strand at 159.349 kb on - strand at 159.349 kb on - strand at 159.349 kb on - strand at 159.349 kb on - strand at 159.352 kb on + strand at 159.352 kb on + strand at 159.352 kb on + strand at 159.353 kb on - strand at 159.353 kb on - strand at 159.353 kb on - strand at 159.353 kb on - strand at 159.353 kb on - strand at 159.353 kb on - strand at 159.353 kb on - strand at 159.353 kb on - strand at 159.398 kb on - strand at 159.398 kb on - strand at 159.409 kb on + strand at 159.409 kb on + strand at 159.409 kb on + strand at 159.409 kb on + strand at 159.410 kb on - strand at 159.410 kb on - strand at 159.410 kb on - strand at 159.486 kb on - strand at 159.674 kb on + strand, within OKGIIK_00730 at 159.674 kb on + strand, within OKGIIK_00730 at 159.674 kb on + strand, within OKGIIK_00730 at 159.675 kb on - strand, within OKGIIK_00730 at 159.708 kb on + strand, within OKGIIK_00730 at 159.708 kb on + strand, within OKGIIK_00730 at 159.709 kb on - strand, within OKGIIK_00730 at 159.709 kb on - strand, within OKGIIK_00730 at 159.723 kb on + strand, within OKGIIK_00730 at 159.723 kb on + strand, within OKGIIK_00730 at 159.723 kb on + strand, within OKGIIK_00730 at 159.723 kb on + strand, within OKGIIK_00730 at 159.724 kb on - strand, within OKGIIK_00730 at 159.724 kb on - strand, within OKGIIK_00730 at 159.741 kb on + strand, within OKGIIK_00730 at 159.741 kb on + strand, within OKGIIK_00730 at 159.741 kb on + strand, within OKGIIK_00730 at 159.742 kb on - strand, within OKGIIK_00730 at 159.742 kb on - strand, within OKGIIK_00730 at 159.742 kb on - strand, within OKGIIK_00730
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 5 mM remove 157,442 + OKGIIK_00715 0.66 -0.1 157,442 + OKGIIK_00715 0.66 -3.0 157,443 - OKGIIK_00715 0.66 -3.4 157,443 - OKGIIK_00715 0.66 -1.1 157,443 - OKGIIK_00715 0.66 -1.4 157,443 - OKGIIK_00715 0.66 +0.9 157,443 - OKGIIK_00715 0.66 -1.5 157,443 - OKGIIK_00715 0.66 -1.9 157,597 + -1.1 157,597 + -1.9 157,597 + -0.9 157,597 + -1.5 157,598 - -3.8 157,598 - -1.1 157,616 + -0.1 157,617 - -1.1 157,624 + +0.3 157,624 + -0.7 157,625 - -0.9 157,625 - +0.0 157,647 + -1.9 157,647 + -0.7 157,647 + -0.1 157,647 + +2.5 157,648 - -1.1 157,659 + -0.4 157,773 + ygjP OKGIIK_00720 0.27 -0.9 157,773 + ygjP OKGIIK_00720 0.27 -1.1 157,773 + ygjP OKGIIK_00720 0.27 -1.5 157,773 + ygjP OKGIIK_00720 0.27 -1.1 157,774 - ygjP OKGIIK_00720 0.27 -0.1 157,774 - ygjP OKGIIK_00720 0.27 +0.9 157,774 - ygjP OKGIIK_00720 0.27 -0.1 157,779 + ygjP OKGIIK_00720 0.28 -2.5 157,779 + ygjP OKGIIK_00720 0.28 +0.3 157,779 + ygjP OKGIIK_00720 0.28 -0.7 157,780 - ygjP OKGIIK_00720 0.29 -0.7 157,780 - ygjP OKGIIK_00720 0.29 +1.2 157,780 - ygjP OKGIIK_00720 0.29 -0.9 157,780 - ygjP OKGIIK_00720 0.29 -0.7 157,780 - ygjP OKGIIK_00720 0.29 -0.1 157,839 + ygjP OKGIIK_00720 0.40 -1.8 157,839 + ygjP OKGIIK_00720 0.40 +1.1 157,839 + ygjP OKGIIK_00720 0.40 +0.3 157,839 + ygjP OKGIIK_00720 0.40 -0.8 157,840 - ygjP OKGIIK_00720 0.40 -0.9 157,840 - ygjP OKGIIK_00720 0.40 -1.1 157,840 - ygjP OKGIIK_00720 0.40 +0.3 157,840 - ygjP OKGIIK_00720 0.40 -0.7 158,037 + ygjP OKGIIK_00720 0.78 -0.4 158,037 + ygjP OKGIIK_00720 0.78 +0.1 158,037 + ygjP OKGIIK_00720 0.78 -2.4 158,037 + ygjP OKGIIK_00720 0.78 -2.5 158,037 + ygjP OKGIIK_00720 0.78 -2.2 158,037 + ygjP OKGIIK_00720 0.78 +1.9 158,037 + ygjP OKGIIK_00720 0.78 +1.2 158,037 + ygjP OKGIIK_00720 0.78 +0.9 158,052 + ygjP OKGIIK_00720 0.81 -1.1 158,052 + ygjP OKGIIK_00720 0.81 -0.6 158,052 + ygjP OKGIIK_00720 0.81 +1.9 158,052 + ygjP OKGIIK_00720 0.81 -2.6 158,052 + ygjP OKGIIK_00720 0.81 -1.4 158,052 + ygjP OKGIIK_00720 0.81 -2.1 158,052 + ygjP OKGIIK_00720 0.81 -1.7 158,052 + ygjP OKGIIK_00720 0.81 -2.2 158,052 + ygjP OKGIIK_00720 0.81 -1.2 158,052 + ygjP OKGIIK_00720 0.81 -1.9 158,052 + ygjP OKGIIK_00720 0.81 -1.5 158,053 - ygjP OKGIIK_00720 0.81 +0.9 158,053 - ygjP OKGIIK_00720 0.81 -0.7 158,053 - ygjP OKGIIK_00720 0.81 -1.1 158,053 - ygjP OKGIIK_00720 0.81 -0.5 158,053 - ygjP OKGIIK_00720 0.81 -1.0 158,053 - ygjP OKGIIK_00720 0.81 -1.5 158,053 - ygjP OKGIIK_00720 0.81 -1.8 158,053 - ygjP OKGIIK_00720 0.81 -3.5 158,067 + ygjP OKGIIK_00720 0.84 -0.9 158,067 + ygjP OKGIIK_00720 0.84 -0.1 158,068 - ygjP OKGIIK_00720 0.84 -1.1 158,097 + ygjP OKGIIK_00720 0.90 +1.9 158,097 + ygjP OKGIIK_00720 0.90 -1.8 158,097 + ygjP OKGIIK_00720 0.90 -0.4 158,098 - ygjP OKGIIK_00720 0.90 -2.4 158,098 - ygjP OKGIIK_00720 0.90 -0.1 158,177 + -1.5 158,177 + -0.9 158,177 + -1.1 158,177 + -0.4 158,177 + -0.4 158,177 + -0.7 158,178 - -2.6 158,178 - -1.7 158,178 - -1.1 158,178 - -0.1 158,450 + OKGIIK_00725 0.46 -2.4 158,450 + OKGIIK_00725 0.46 -2.8 158,450 + OKGIIK_00725 0.46 -1.9 158,450 + OKGIIK_00725 0.46 -1.6 158,450 + OKGIIK_00725 0.46 +0.9 158,450 + OKGIIK_00725 0.46 -1.5 158,450 + OKGIIK_00725 0.46 -0.9 158,451 - OKGIIK_00725 0.46 -1.2 158,451 - OKGIIK_00725 0.46 -0.7 158,451 - OKGIIK_00725 0.46 -1.5 158,753 + +2.9 158,753 + -1.4 158,753 + +0.9 158,753 + -1.7 158,754 - -2.8 158,754 - -2.6 158,792 + -0.9 158,985 - -0.4 158,985 - +0.3 158,985 - -1.1 159,013 + +1.9 159,013 + -2.2 159,013 + -1.1 159,013 + -0.1 159,014 - -0.7 159,014 - -2.0 159,014 - -1.5 159,014 - -2.0 159,014 - +0.0 159,036 + +0.9 159,119 + -0.4 159,119 + -1.9 159,222 + -2.2 159,222 + -2.2 159,222 + -0.9 159,222 + +1.3 159,223 - -2.0 159,223 - +1.0 159,223 - -1.2 159,223 - -1.2 159,223 - -0.7 159,223 - -1.7 159,223 - -1.8 159,223 - +1.9 159,223 - -2.2 159,223 - +0.9 159,223 - +1.9 159,317 + +0.9 159,317 + -0.9 159,317 + -0.4 159,318 - -2.6 159,318 - -1.2 159,318 - -1.9 159,318 - -1.2 159,346 + -3.0 159,346 + +1.9 159,346 + -1.9 159,346 + -0.7 159,346 + -1.5 159,347 - +0.7 159,347 - -2.1 159,347 - +0.7 159,347 - -1.8 159,347 - -2.8 159,347 - -0.4 159,347 - -0.9 159,347 - +0.5 159,347 - +0.5 159,348 + -0.1 159,348 + -0.9 159,348 + -3.7 159,348 + +0.1 159,348 + -2.5 159,348 + -0.1 159,348 + -1.8 159,349 - -0.1 159,349 - -2.5 159,349 - -0.1 159,349 - +0.8 159,349 - +0.9 159,349 - -1.2 159,349 - -1.3 159,349 - -0.1 159,349 - -1.7 159,349 - -0.7 159,352 + -3.4 159,352 + -0.7 159,352 + -3.1 159,353 - -1.7 159,353 - -1.5 159,353 - -2.2 159,353 - -1.8 159,353 - +2.9 159,353 - -0.1 159,353 - -1.4 159,353 - -1.5 159,398 - -0.7 159,398 - -0.1 159,409 + -2.1 159,409 + +1.9 159,409 + -0.7 159,409 + +0.3 159,410 - +0.3 159,410 - -1.7 159,410 - +0.3 159,486 - -1.8 159,674 + OKGIIK_00730 0.18 +3.5 159,674 + OKGIIK_00730 0.18 -1.9 159,674 + OKGIIK_00730 0.18 -0.9 159,675 - OKGIIK_00730 0.18 -0.4 159,708 + OKGIIK_00730 0.20 -2.1 159,708 + OKGIIK_00730 0.20 -0.7 159,709 - OKGIIK_00730 0.20 -0.1 159,709 - OKGIIK_00730 0.20 -0.1 159,723 + OKGIIK_00730 0.21 -1.4 159,723 + OKGIIK_00730 0.21 -1.4 159,723 + OKGIIK_00730 0.21 -1.0 159,723 + OKGIIK_00730 0.21 +0.3 159,724 - OKGIIK_00730 0.21 -3.3 159,724 - OKGIIK_00730 0.21 -2.1 159,741 + OKGIIK_00730 0.22 -0.5 159,741 + OKGIIK_00730 0.22 -1.2 159,741 + OKGIIK_00730 0.22 -1.9 159,742 - OKGIIK_00730 0.22 -1.1 159,742 - OKGIIK_00730 0.22 -0.9 159,742 - OKGIIK_00730 0.22 -0.1
Or see this region's nucleotide sequence