Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nusG and secE are separated by 7 nucleotides secE and Trp_trna are separated by 82 nucleotides Trp_trna and tuf are separated by 192 nucleotides tuf and Thr_trna are separated by 77 nucleotides Thr_trna and Gly_trna are separated by 136 nucleotides Gly_trna and Tyr_trna are separated by 18 nucleotides Tyr_trna and yciA are separated by 93 nucleotides
OKGIIK_10525: nusG - transcription termination/antitermination protein NusG, at 2,264,152 to 2,264,712
nusG
OKGIIK_10530: secE - preprotein translocase subunit SecE, at 2,264,720 to 2,265,115
secE
OKGIIK_10535: Trp_trna - tRNA-Trp, at 2,265,198 to 2,265,273
_trna
OKGIIK_10540: tuf - elongation factor Tu, at 2,265,466 to 2,266,656
tuf
OKGIIK_10545: Thr_trna - tRNA-Thr, at 2,266,734 to 2,266,809
_trna
OKGIIK_10550: Gly_trna - tRNA-Gly, at 2,266,946 to 2,267,019
_trna
OKGIIK_10555: Tyr_trna - tRNA-Tyr, at 2,267,038 to 2,267,123
_trna
OKGIIK_10560: yciA - acyl-CoA thioesterase, at 2,267,217 to 2,267,705
yciA
Position (kb)
2265
2266
2267 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2265.335 kb on - strand at 2265.335 kb on - strand at 2265.335 kb on - strand at 2265.399 kb on - strand at 2265.399 kb on - strand at 2265.399 kb on - strand at 2265.399 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.657 kb on - strand, within tuf at 2265.657 kb on - strand, within tuf at 2265.657 kb on - strand, within tuf at 2265.691 kb on - strand, within tuf at 2265.691 kb on - strand, within tuf at 2265.691 kb on - strand, within tuf at 2265.691 kb on - strand, within tuf at 2266.814 kb on - strand at 2266.814 kb on - strand at 2266.814 kb on - strand at 2266.814 kb on - strand at 2266.814 kb on - strand at 2266.814 kb on - strand at 2266.814 kb on - strand at 2266.814 kb on - strand at 2266.814 kb on - strand at 2266.814 kb on - strand at 2266.814 kb on - strand at 2266.814 kb on - strand at 2266.814 kb on - strand at 2266.814 kb on - strand at 2266.939 kb on - strand at 2266.939 kb on - strand at 2266.939 kb on - strand at 2266.939 kb on - strand at 2266.939 kb on - strand at 2266.939 kb on - strand at 2266.939 kb on - strand at 2267.292 kb on + strand, within yciA at 2267.293 kb on - strand, within yciA at 2267.293 kb on - strand, within yciA at 2267.293 kb on - strand, within yciA at 2267.293 kb on - strand, within yciA at 2267.293 kb on - strand, within yciA at 2267.293 kb on - strand, within yciA at 2267.293 kb on - strand, within yciA at 2267.293 kb on - strand, within yciA at 2267.560 kb on + strand, within yciA at 2267.560 kb on + strand, within yciA at 2267.560 kb on + strand, within yciA at 2267.560 kb on + strand, within yciA at 2267.560 kb on + strand, within yciA at 2267.560 kb on + strand, within yciA at 2267.560 kb on + strand, within yciA at 2267.560 kb on + strand, within yciA at 2267.560 kb on + strand, within yciA at 2267.561 kb on - strand, within yciA at 2267.561 kb on - strand, within yciA at 2267.561 kb on - strand, within yciA at 2267.561 kb on - strand, within yciA at 2267.561 kb on - strand, within yciA at 2267.590 kb on + strand, within yciA
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 2,265,335 - -1.0 2,265,335 - -0.4 2,265,335 - +0.3 2,265,399 - -0.4 2,265,399 - -2.3 2,265,399 - -0.4 2,265,399 - -0.8 2,265,468 - -1.1 2,265,468 - +0.1 2,265,468 - +0.2 2,265,468 - -1.3 2,265,468 - -3.3 2,265,468 - -0.8 2,265,468 - -1.2 2,265,468 - -0.4 2,265,468 - -0.0 2,265,468 - -0.4 2,265,468 - -2.1 2,265,468 - -0.0 2,265,468 - -2.4 2,265,468 - -2.8 2,265,468 - -0.0 2,265,468 - -0.8 2,265,468 - -0.8 2,265,468 - -1.4 2,265,468 - +0.8 2,265,468 - -1.8 2,265,468 - -1.2 2,265,468 - -0.4 2,265,468 - -2.7 2,265,468 - -1.7 2,265,468 - -0.4 2,265,657 - tuf OKGIIK_10540 0.16 -2.8 2,265,657 - tuf OKGIIK_10540 0.16 -1.2 2,265,657 - tuf OKGIIK_10540 0.16 -2.0 2,265,691 - tuf OKGIIK_10540 0.19 -2.1 2,265,691 - tuf OKGIIK_10540 0.19 -1.6 2,265,691 - tuf OKGIIK_10540 0.19 -3.4 2,265,691 - tuf OKGIIK_10540 0.19 -1.6 2,266,814 - -2.8 2,266,814 - -1.0 2,266,814 - -2.5 2,266,814 - -1.2 2,266,814 - -1.9 2,266,814 - +0.8 2,266,814 - -2.2 2,266,814 - -1.4 2,266,814 - -0.2 2,266,814 - -1.2 2,266,814 - -0.0 2,266,814 - -0.4 2,266,814 - -0.0 2,266,814 - -1.8 2,266,939 - -2.1 2,266,939 - -2.1 2,266,939 - -1.0 2,266,939 - -1.6 2,266,939 - +0.4 2,266,939 - -1.0 2,266,939 - -0.8 2,267,292 + yciA OKGIIK_10560 0.15 -1.0 2,267,293 - yciA OKGIIK_10560 0.16 -0.8 2,267,293 - yciA OKGIIK_10560 0.16 -0.1 2,267,293 - yciA OKGIIK_10560 0.16 -0.4 2,267,293 - yciA OKGIIK_10560 0.16 -0.0 2,267,293 - yciA OKGIIK_10560 0.16 -1.2 2,267,293 - yciA OKGIIK_10560 0.16 -1.0 2,267,293 - yciA OKGIIK_10560 0.16 -1.0 2,267,293 - yciA OKGIIK_10560 0.16 +0.1 2,267,560 + yciA OKGIIK_10560 0.70 -0.4 2,267,560 + yciA OKGIIK_10560 0.70 -1.4 2,267,560 + yciA OKGIIK_10560 0.70 -0.7 2,267,560 + yciA OKGIIK_10560 0.70 -0.8 2,267,560 + yciA OKGIIK_10560 0.70 -1.9 2,267,560 + yciA OKGIIK_10560 0.70 -0.8 2,267,560 + yciA OKGIIK_10560 0.70 -3.2 2,267,560 + yciA OKGIIK_10560 0.70 -0.0 2,267,560 + yciA OKGIIK_10560 0.70 -0.8 2,267,561 - yciA OKGIIK_10560 0.70 -0.0 2,267,561 - yciA OKGIIK_10560 0.70 -1.6 2,267,561 - yciA OKGIIK_10560 0.70 -1.2 2,267,561 - yciA OKGIIK_10560 0.70 +0.7 2,267,561 - yciA OKGIIK_10560 0.70 -1.8 2,267,590 + yciA OKGIIK_10560 0.76 +0.6
Or see this region's nucleotide sequence