Strain Fitness in Rhodanobacter sp. FW510-T8 around OKGIIK_09230

Experiment: R2A_PIPES with Cobalt chloride 512 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKGIIK_09225 and OKGIIK_09230 are separated by 100 nucleotidesOKGIIK_09230 and recD overlap by 4 nucleotides OKGIIK_09225: OKGIIK_09225 - RecBCD enzyme subunit RecC, at 1,995,426 to 1,998,875 _09225 OKGIIK_09230: OKGIIK_09230 - RecBCD enzyme subunit RecB, at 1,998,976 to 2,002,560 _09230 OKGIIK_09235: recD - exodeoxyribonuclease V subunit alpha, at 2,002,557 to 2,004,587 recD Position (kb) 1998 1999 2000 2001 2002 2003Strain fitness (log2 ratio) -3 -2 -1 0 1at 1998.390 kb on + strand, within OKGIIK_09225at 1998.965 kb on + strandat 1998.965 kb on + strandat 1998.965 kb on + strandat 1998.965 kb on + strandat 1998.965 kb on + strandat 1998.965 kb on + strandat 1998.965 kb on + strandat 1998.965 kb on + strandat 1998.965 kb on + strandat 1998.966 kb on - strandat 1998.966 kb on - strandat 2000.032 kb on + strand, within OKGIIK_09230at 2000.032 kb on + strand, within OKGIIK_09230at 2000.032 kb on + strand, within OKGIIK_09230at 2000.213 kb on - strand, within OKGIIK_09230at 2000.419 kb on + strand, within OKGIIK_09230at 2001.262 kb on + strand, within OKGIIK_09230at 2001.262 kb on + strand, within OKGIIK_09230at 2001.391 kb on + strand, within OKGIIK_09230at 2001.391 kb on + strand, within OKGIIK_09230at 2001.391 kb on + strand, within OKGIIK_09230at 2001.392 kb on - strand, within OKGIIK_09230at 2002.843 kb on - strand, within recD

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM
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1,998,390 + OKGIIK_09225 0.86 -2.9
1,998,965 + -0.7
1,998,965 + +0.5
1,998,965 + +0.5
1,998,965 + -0.1
1,998,965 + -2.3
1,998,965 + -1.6
1,998,965 + +0.5
1,998,965 + -1.2
1,998,965 + -0.8
1,998,966 - +0.7
1,998,966 - -1.1
2,000,032 + OKGIIK_09230 0.29 -1.7
2,000,032 + OKGIIK_09230 0.29 -1.5
2,000,032 + OKGIIK_09230 0.29 +1.5
2,000,213 - OKGIIK_09230 0.35 -2.7
2,000,419 + OKGIIK_09230 0.40 -1.1
2,001,262 + OKGIIK_09230 0.64 -2.3
2,001,262 + OKGIIK_09230 0.64 +0.3
2,001,391 + OKGIIK_09230 0.67 -3.7
2,001,391 + OKGIIK_09230 0.67 -0.8
2,001,391 + OKGIIK_09230 0.67 -0.8
2,001,392 - OKGIIK_09230 0.67 -2.0
2,002,843 - recD OKGIIK_09235 0.14 -0.5

Or see this region's nucleotide sequence