Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pheA and aroA overlap by 4 nucleotides aroA and kdsB are separated by 53 nucleotides kdsB and uvrC are separated by 39 nucleotides
OKGIIK_06915: pheA - prephenate dehydratase, at 1,536,314 to 1,537,402
pheA
OKGIIK_06920: aroA - 3-phosphoshikimate 1-carboxyvinyltransferase, at 1,537,399 to 1,538,709
aroA
OKGIIK_06925: kdsB - 3-deoxy-manno-octulosonate cytidylyltransferase, at 1,538,763 to 1,539,542
kdsB
OKGIIK_06930: uvrC - excinuclease ABC subunit UvrC, at 1,539,582 to 1,541,408
uvrC
Position (kb)
1537
1538
1539 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1536.458 kb on + strand, within pheA at 1536.458 kb on + strand, within pheA at 1536.458 kb on + strand, within pheA at 1536.458 kb on + strand, within pheA at 1536.458 kb on + strand, within pheA at 1536.458 kb on + strand, within pheA at 1536.458 kb on + strand, within pheA at 1536.458 kb on + strand, within pheA at 1536.458 kb on + strand, within pheA at 1536.459 kb on - strand, within pheA at 1536.696 kb on + strand, within pheA at 1536.696 kb on + strand, within pheA at 1536.696 kb on + strand, within pheA at 1536.696 kb on + strand, within pheA at 1536.696 kb on + strand, within pheA at 1536.697 kb on - strand, within pheA at 1536.697 kb on - strand, within pheA at 1536.697 kb on - strand, within pheA at 1536.697 kb on - strand, within pheA at 1536.697 kb on - strand, within pheA at 1536.697 kb on - strand, within pheA at 1536.805 kb on + strand, within pheA at 1536.805 kb on + strand, within pheA at 1536.805 kb on + strand, within pheA at 1536.805 kb on + strand, within pheA at 1536.805 kb on + strand, within pheA at 1536.805 kb on + strand, within pheA at 1536.805 kb on + strand, within pheA at 1536.805 kb on + strand, within pheA at 1536.805 kb on + strand, within pheA at 1536.806 kb on - strand, within pheA at 1536.806 kb on - strand, within pheA at 1536.806 kb on - strand, within pheA at 1536.888 kb on - strand, within pheA at 1537.046 kb on + strand, within pheA at 1537.193 kb on + strand, within pheA at 1537.193 kb on + strand, within pheA at 1537.193 kb on + strand, within pheA at 1537.193 kb on + strand, within pheA at 1537.193 kb on + strand, within pheA at 1537.193 kb on + strand, within pheA at 1537.220 kb on + strand, within pheA at 1537.277 kb on + strand, within pheA at 1537.278 kb on - strand, within pheA at 1537.278 kb on - strand, within pheA at 1537.383 kb on - strand at 1537.945 kb on + strand, within aroA at 1537.996 kb on - strand, within aroA at 1538.306 kb on + strand, within aroA at 1538.307 kb on - strand, within aroA at 1538.307 kb on - strand, within aroA at 1538.307 kb on - strand, within aroA at 1538.718 kb on + strand at 1538.718 kb on + strand at 1538.719 kb on - strand at 1538.733 kb on + strand at 1538.733 kb on + strand at 1538.733 kb on + strand at 1538.734 kb on - strand at 1538.734 kb on - strand at 1538.734 kb on - strand at 1538.734 kb on - strand at 1539.570 kb on + strand at 1539.570 kb on + strand at 1539.570 kb on + strand at 1539.571 kb on - strand at 1539.571 kb on - strand at 1539.571 kb on - strand at 1539.654 kb on + strand at 1539.654 kb on + strand at 1539.654 kb on + strand at 1539.655 kb on - strand at 1539.687 kb on + strand at 1539.687 kb on + strand at 1539.687 kb on + strand at 1539.687 kb on + strand at 1539.688 kb on - strand at 1539.688 kb on - strand at 1539.688 kb on - strand at 1539.688 kb on - strand at 1539.688 kb on - strand at 1539.688 kb on - strand at 1539.688 kb on - strand at 1539.688 kb on - strand at 1539.688 kb on - strand at 1539.688 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 1,536,458 + pheA OKGIIK_06915 0.13 -0.5 1,536,458 + pheA OKGIIK_06915 0.13 -1.9 1,536,458 + pheA OKGIIK_06915 0.13 -0.0 1,536,458 + pheA OKGIIK_06915 0.13 -0.0 1,536,458 + pheA OKGIIK_06915 0.13 -1.3 1,536,458 + pheA OKGIIK_06915 0.13 -0.8 1,536,458 + pheA OKGIIK_06915 0.13 -0.2 1,536,458 + pheA OKGIIK_06915 0.13 -0.5 1,536,458 + pheA OKGIIK_06915 0.13 -1.8 1,536,459 - pheA OKGIIK_06915 0.13 -2.5 1,536,696 + pheA OKGIIK_06915 0.35 -2.2 1,536,696 + pheA OKGIIK_06915 0.35 -1.3 1,536,696 + pheA OKGIIK_06915 0.35 -1.0 1,536,696 + pheA OKGIIK_06915 0.35 -1.9 1,536,696 + pheA OKGIIK_06915 0.35 -0.8 1,536,697 - pheA OKGIIK_06915 0.35 -0.8 1,536,697 - pheA OKGIIK_06915 0.35 -0.0 1,536,697 - pheA OKGIIK_06915 0.35 -1.3 1,536,697 - pheA OKGIIK_06915 0.35 -0.8 1,536,697 - pheA OKGIIK_06915 0.35 +2.1 1,536,697 - pheA OKGIIK_06915 0.35 +1.0 1,536,805 + pheA OKGIIK_06915 0.45 -0.6 1,536,805 + pheA OKGIIK_06915 0.45 -0.0 1,536,805 + pheA OKGIIK_06915 0.45 +1.0 1,536,805 + pheA OKGIIK_06915 0.45 +0.3 1,536,805 + pheA OKGIIK_06915 0.45 +0.2 1,536,805 + pheA OKGIIK_06915 0.45 -1.4 1,536,805 + pheA OKGIIK_06915 0.45 -2.2 1,536,805 + pheA OKGIIK_06915 0.45 -0.5 1,536,805 + pheA OKGIIK_06915 0.45 -1.3 1,536,806 - pheA OKGIIK_06915 0.45 -1.6 1,536,806 - pheA OKGIIK_06915 0.45 -0.0 1,536,806 - pheA OKGIIK_06915 0.45 -1.6 1,536,888 - pheA OKGIIK_06915 0.53 -1.0 1,537,046 + pheA OKGIIK_06915 0.67 -2.2 1,537,193 + pheA OKGIIK_06915 0.81 -2.7 1,537,193 + pheA OKGIIK_06915 0.81 -1.0 1,537,193 + pheA OKGIIK_06915 0.81 -0.8 1,537,193 + pheA OKGIIK_06915 0.81 +0.7 1,537,193 + pheA OKGIIK_06915 0.81 -0.5 1,537,193 + pheA OKGIIK_06915 0.81 -1.2 1,537,220 + pheA OKGIIK_06915 0.83 -0.8 1,537,277 + pheA OKGIIK_06915 0.88 -1.4 1,537,278 - pheA OKGIIK_06915 0.89 -1.0 1,537,278 - pheA OKGIIK_06915 0.89 -0.8 1,537,383 - -3.0 1,537,945 + aroA OKGIIK_06920 0.42 -2.5 1,537,996 - aroA OKGIIK_06920 0.46 +1.5 1,538,306 + aroA OKGIIK_06920 0.69 -1.5 1,538,307 - aroA OKGIIK_06920 0.69 -1.5 1,538,307 - aroA OKGIIK_06920 0.69 -1.8 1,538,307 - aroA OKGIIK_06920 0.69 +0.5 1,538,718 + -1.8 1,538,718 + -0.2 1,538,719 - -0.5 1,538,733 + -0.5 1,538,733 + +0.5 1,538,733 + +0.1 1,538,734 - +1.5 1,538,734 - +0.2 1,538,734 - -1.5 1,538,734 - -0.5 1,539,570 + -1.2 1,539,570 + -0.5 1,539,570 + -0.1 1,539,571 - -0.5 1,539,571 - +1.0 1,539,571 - -0.1 1,539,654 + -0.1 1,539,654 + -2.1 1,539,654 + -1.3 1,539,655 - -1.3 1,539,687 + -0.8 1,539,687 + -2.2 1,539,687 + -2.9 1,539,687 + -0.1 1,539,688 - -1.5 1,539,688 - -1.9 1,539,688 - +0.0 1,539,688 - -1.9 1,539,688 - -1.1 1,539,688 - -1.5 1,539,688 - -2.6 1,539,688 - -2.5 1,539,688 - -2.4 1,539,688 - +0.5
Or see this region's nucleotide sequence