Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nlpA and metP are separated by 227 nucleotides metP and abcC overlap by 4 nucleotides
OKGIIK_03550: nlpA - methionine ABC transporter substrate-binding protein, at 787,191 to 787,985
nlpA
OKGIIK_03555: metP - metal ABC transporter permease, at 788,213 to 788,890
metP
OKGIIK_03560: abcC - methionine ABC transporter ATP-binding protein, at 788,887 to 789,897
abcC
Position (kb) 788
789 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 787.219 kb on + strand at 787.219 kb on + strand at 787.219 kb on + strand at 787.219 kb on + strand at 787.219 kb on + strand at 787.219 kb on + strand at 787.219 kb on + strand at 787.219 kb on - strand at 787.220 kb on - strand at 787.220 kb on - strand at 787.220 kb on - strand at 787.220 kb on - strand at 787.220 kb on - strand at 787.318 kb on + strand, within nlpA at 787.318 kb on + strand, within nlpA at 787.318 kb on + strand, within nlpA at 787.318 kb on + strand, within nlpA at 787.318 kb on + strand, within nlpA at 787.318 kb on + strand, within nlpA at 787.319 kb on - strand, within nlpA at 787.319 kb on - strand, within nlpA at 787.319 kb on - strand, within nlpA at 787.319 kb on - strand, within nlpA at 787.319 kb on - strand, within nlpA at 787.319 kb on - strand, within nlpA at 787.319 kb on - strand, within nlpA at 787.327 kb on + strand, within nlpA at 787.327 kb on + strand, within nlpA at 787.328 kb on - strand, within nlpA at 787.328 kb on - strand, within nlpA at 787.328 kb on - strand, within nlpA at 787.328 kb on - strand, within nlpA at 787.328 kb on - strand, within nlpA at 787.392 kb on + strand, within nlpA at 787.393 kb on + strand, within nlpA at 787.393 kb on + strand, within nlpA at 787.393 kb on + strand, within nlpA at 787.393 kb on + strand, within nlpA at 787.393 kb on + strand, within nlpA at 787.393 kb on + strand, within nlpA at 787.393 kb on + strand, within nlpA at 787.394 kb on - strand, within nlpA at 787.394 kb on - strand, within nlpA at 787.394 kb on - strand, within nlpA at 787.394 kb on - strand, within nlpA at 787.394 kb on - strand, within nlpA at 787.639 kb on + strand, within nlpA at 787.639 kb on + strand, within nlpA at 787.639 kb on + strand, within nlpA at 787.640 kb on - strand, within nlpA at 787.640 kb on - strand, within nlpA at 787.640 kb on - strand, within nlpA at 787.640 kb on - strand, within nlpA at 787.652 kb on - strand, within nlpA at 787.729 kb on + strand, within nlpA at 787.730 kb on - strand, within nlpA at 787.730 kb on - strand, within nlpA at 787.747 kb on + strand, within nlpA at 787.747 kb on + strand, within nlpA at 787.747 kb on + strand, within nlpA at 787.747 kb on + strand, within nlpA at 787.747 kb on + strand, within nlpA at 787.747 kb on + strand, within nlpA at 787.747 kb on + strand, within nlpA at 787.748 kb on - strand, within nlpA at 787.795 kb on + strand, within nlpA at 787.796 kb on - strand, within nlpA at 787.990 kb on + strand at 787.990 kb on + strand at 787.990 kb on + strand at 787.990 kb on + strand at 787.991 kb on - strand at 787.991 kb on - strand at 787.991 kb on - strand at 788.015 kb on + strand at 788.015 kb on + strand at 788.015 kb on + strand at 788.016 kb on - strand at 788.024 kb on + strand at 788.024 kb on + strand at 788.025 kb on - strand at 788.025 kb on - strand at 788.025 kb on - strand at 788.025 kb on - strand at 788.025 kb on - strand at 788.025 kb on - strand at 788.027 kb on - strand at 788.053 kb on + strand at 788.053 kb on + strand at 788.053 kb on + strand at 788.053 kb on + strand at 788.053 kb on + strand at 788.053 kb on + strand at 788.053 kb on + strand at 788.054 kb on - strand at 788.054 kb on - strand at 788.054 kb on - strand at 788.054 kb on - strand at 788.054 kb on - strand at 788.054 kb on - strand at 788.054 kb on - strand at 788.089 kb on - strand at 788.178 kb on + strand at 788.178 kb on + strand at 788.178 kb on + strand at 788.179 kb on - strand at 788.179 kb on - strand at 788.179 kb on - strand at 788.179 kb on - strand at 788.179 kb on - strand at 788.224 kb on + strand at 788.224 kb on + strand at 788.224 kb on + strand at 788.224 kb on + strand at 788.224 kb on + strand at 788.225 kb on - strand at 788.225 kb on - strand at 788.225 kb on - strand at 788.225 kb on - strand at 788.225 kb on - strand at 788.225 kb on - strand at 788.225 kb on - strand at 788.225 kb on - strand at 788.225 kb on - strand at 788.226 kb on + strand at 788.226 kb on + strand at 788.227 kb on - strand at 788.227 kb on - strand at 788.304 kb on + strand, within metP at 788.304 kb on + strand, within metP at 788.304 kb on + strand, within metP at 788.304 kb on + strand, within metP at 788.304 kb on + strand, within metP at 788.304 kb on + strand, within metP at 788.305 kb on - strand, within metP at 788.305 kb on - strand, within metP at 788.305 kb on - strand, within metP at 788.305 kb on - strand, within metP at 788.305 kb on - strand, within metP at 788.305 kb on - strand, within metP at 788.305 kb on - strand, within metP at 788.305 kb on - strand, within metP at 788.316 kb on + strand, within metP at 788.316 kb on + strand, within metP at 788.316 kb on + strand, within metP at 788.317 kb on - strand, within metP at 788.317 kb on - strand, within metP at 788.317 kb on - strand, within metP at 788.317 kb on - strand, within metP at 788.317 kb on - strand, within metP at 788.317 kb on - strand, within metP at 788.317 kb on - strand, within metP at 788.326 kb on - strand, within metP at 788.338 kb on - strand, within metP at 788.338 kb on - strand, within metP at 788.388 kb on + strand, within metP at 788.628 kb on + strand, within metP at 788.628 kb on + strand, within metP at 788.628 kb on + strand, within metP at 788.628 kb on + strand, within metP at 788.629 kb on - strand, within metP at 788.629 kb on - strand, within metP at 788.629 kb on - strand, within metP at 788.629 kb on - strand, within metP at 788.629 kb on - strand, within metP at 788.629 kb on - strand, within metP at 788.629 kb on - strand, within metP at 788.629 kb on - strand, within metP at 788.629 kb on - strand, within metP at 788.709 kb on + strand, within metP at 788.710 kb on - strand, within metP at 788.710 kb on - strand, within metP at 788.710 kb on - strand, within metP at 788.751 kb on + strand, within metP at 788.752 kb on - strand, within metP at 788.752 kb on - strand, within metP at 788.752 kb on - strand, within metP at 788.752 kb on - strand, within metP at 788.752 kb on - strand, within metP at 788.752 kb on - strand, within metP at 789.274 kb on + strand, within abcC at 789.274 kb on + strand, within abcC at 789.485 kb on + strand, within abcC at 789.486 kb on - strand, within abcC at 789.486 kb on - strand, within abcC at 789.486 kb on - strand, within abcC at 789.754 kb on + strand, within abcC at 789.754 kb on + strand, within abcC at 789.754 kb on + strand, within abcC at 789.755 kb on - strand, within abcC at 789.755 kb on - strand, within abcC at 789.867 kb on - strand at 789.867 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 787,219 + -4.2 787,219 + -2.2 787,219 + -1.6 787,219 + +0.3 787,219 + -2.4 787,219 + -2.8 787,219 + -1.7 787,219 - -1.5 787,220 - -1.5 787,220 - -1.3 787,220 - -0.8 787,220 - -0.2 787,220 - -2.4 787,318 + nlpA OKGIIK_03550 0.16 -1.5 787,318 + nlpA OKGIIK_03550 0.16 -2.3 787,318 + nlpA OKGIIK_03550 0.16 -1.7 787,318 + nlpA OKGIIK_03550 0.16 -0.5 787,318 + nlpA OKGIIK_03550 0.16 +0.2 787,318 + nlpA OKGIIK_03550 0.16 -0.5 787,319 - nlpA OKGIIK_03550 0.16 -1.7 787,319 - nlpA OKGIIK_03550 0.16 -0.3 787,319 - nlpA OKGIIK_03550 0.16 -2.4 787,319 - nlpA OKGIIK_03550 0.16 -0.5 787,319 - nlpA OKGIIK_03550 0.16 -1.3 787,319 - nlpA OKGIIK_03550 0.16 -1.8 787,319 - nlpA OKGIIK_03550 0.16 -0.8 787,327 + nlpA OKGIIK_03550 0.17 -2.3 787,327 + nlpA OKGIIK_03550 0.17 -2.2 787,328 - nlpA OKGIIK_03550 0.17 -1.8 787,328 - nlpA OKGIIK_03550 0.17 -0.8 787,328 - nlpA OKGIIK_03550 0.17 -2.7 787,328 - nlpA OKGIIK_03550 0.17 -1.7 787,328 - nlpA OKGIIK_03550 0.17 -1.8 787,392 + nlpA OKGIIK_03550 0.25 -1.8 787,393 + nlpA OKGIIK_03550 0.25 -1.5 787,393 + nlpA OKGIIK_03550 0.25 -0.5 787,393 + nlpA OKGIIK_03550 0.25 +0.5 787,393 + nlpA OKGIIK_03550 0.25 -1.1 787,393 + nlpA OKGIIK_03550 0.25 -0.8 787,393 + nlpA OKGIIK_03550 0.25 -2.1 787,393 + nlpA OKGIIK_03550 0.25 -1.3 787,394 - nlpA OKGIIK_03550 0.26 -0.1 787,394 - nlpA OKGIIK_03550 0.26 -1.3 787,394 - nlpA OKGIIK_03550 0.26 -2.0 787,394 - nlpA OKGIIK_03550 0.26 -4.2 787,394 - nlpA OKGIIK_03550 0.26 -1.1 787,639 + nlpA OKGIIK_03550 0.56 +1.5 787,639 + nlpA OKGIIK_03550 0.56 -2.0 787,639 + nlpA OKGIIK_03550 0.56 -2.1 787,640 - nlpA OKGIIK_03550 0.56 +0.2 787,640 - nlpA OKGIIK_03550 0.56 -0.9 787,640 - nlpA OKGIIK_03550 0.56 -2.2 787,640 - nlpA OKGIIK_03550 0.56 -2.5 787,652 - nlpA OKGIIK_03550 0.58 -0.8 787,729 + nlpA OKGIIK_03550 0.68 -2.7 787,730 - nlpA OKGIIK_03550 0.68 +0.5 787,730 - nlpA OKGIIK_03550 0.68 +0.5 787,747 + nlpA OKGIIK_03550 0.70 -2.1 787,747 + nlpA OKGIIK_03550 0.70 -2.4 787,747 + nlpA OKGIIK_03550 0.70 -0.1 787,747 + nlpA OKGIIK_03550 0.70 -1.0 787,747 + nlpA OKGIIK_03550 0.70 -0.1 787,747 + nlpA OKGIIK_03550 0.70 +0.2 787,747 + nlpA OKGIIK_03550 0.70 -1.3 787,748 - nlpA OKGIIK_03550 0.70 +0.5 787,795 + nlpA OKGIIK_03550 0.76 -0.1 787,796 - nlpA OKGIIK_03550 0.76 +1.5 787,990 + -1.8 787,990 + -1.1 787,990 + -0.1 787,990 + -0.8 787,991 - -0.8 787,991 - -0.9 787,991 - -0.8 788,015 + -1.5 788,015 + +0.2 788,015 + +0.2 788,016 - +0.5 788,024 + -2.7 788,024 + -1.3 788,025 - +2.5 788,025 - +1.5 788,025 - -1.5 788,025 - -2.4 788,025 - -1.7 788,025 - -1.8 788,027 - -0.8 788,053 + +1.5 788,053 + -1.7 788,053 + -1.0 788,053 + +0.1 788,053 + -0.7 788,053 + +0.2 788,053 + -1.0 788,054 - -1.5 788,054 - -0.5 788,054 - -2.0 788,054 - +0.1 788,054 - +2.1 788,054 - +1.5 788,054 - -0.1 788,089 - -2.1 788,178 + -1.8 788,178 + -3.5 788,178 + -1.3 788,179 - -0.1 788,179 - -0.5 788,179 - -1.7 788,179 - -1.5 788,179 - -1.7 788,224 + -2.1 788,224 + -3.5 788,224 + -3.2 788,224 + -1.7 788,224 + -3.2 788,225 - -1.3 788,225 - +1.2 788,225 - -0.5 788,225 - -1.1 788,225 - -2.2 788,225 - -1.1 788,225 - -1.3 788,225 - -0.7 788,225 - -1.1 788,226 + -1.0 788,226 + -0.1 788,227 - -1.5 788,227 - +2.5 788,304 + metP OKGIIK_03555 0.13 -0.3 788,304 + metP OKGIIK_03555 0.13 -2.7 788,304 + metP OKGIIK_03555 0.13 -1.1 788,304 + metP OKGIIK_03555 0.13 -0.5 788,304 + metP OKGIIK_03555 0.13 -0.5 788,304 + metP OKGIIK_03555 0.13 +0.5 788,305 - metP OKGIIK_03555 0.14 -2.0 788,305 - metP OKGIIK_03555 0.14 -1.5 788,305 - metP OKGIIK_03555 0.14 -1.5 788,305 - metP OKGIIK_03555 0.14 -1.8 788,305 - metP OKGIIK_03555 0.14 -1.1 788,305 - metP OKGIIK_03555 0.14 -0.6 788,305 - metP OKGIIK_03555 0.14 +1.5 788,305 - metP OKGIIK_03555 0.14 -1.4 788,316 + metP OKGIIK_03555 0.15 -1.3 788,316 + metP OKGIIK_03555 0.15 -1.1 788,316 + metP OKGIIK_03555 0.15 -3.6 788,317 - metP OKGIIK_03555 0.15 -2.5 788,317 - metP OKGIIK_03555 0.15 -0.1 788,317 - metP OKGIIK_03555 0.15 -3.2 788,317 - metP OKGIIK_03555 0.15 -0.1 788,317 - metP OKGIIK_03555 0.15 +1.5 788,317 - metP OKGIIK_03555 0.15 -1.3 788,317 - metP OKGIIK_03555 0.15 +1.5 788,326 - metP OKGIIK_03555 0.17 +0.5 788,338 - metP OKGIIK_03555 0.18 -1.5 788,338 - metP OKGIIK_03555 0.18 -1.1 788,388 + metP OKGIIK_03555 0.26 -0.1 788,628 + metP OKGIIK_03555 0.61 -1.1 788,628 + metP OKGIIK_03555 0.61 -1.3 788,628 + metP OKGIIK_03555 0.61 -2.1 788,628 + metP OKGIIK_03555 0.61 -1.3 788,629 - metP OKGIIK_03555 0.61 -1.0 788,629 - metP OKGIIK_03555 0.61 -0.8 788,629 - metP OKGIIK_03555 0.61 -1.3 788,629 - metP OKGIIK_03555 0.61 -0.5 788,629 - metP OKGIIK_03555 0.61 -1.1 788,629 - metP OKGIIK_03555 0.61 -1.7 788,629 - metP OKGIIK_03555 0.61 -2.3 788,629 - metP OKGIIK_03555 0.61 -1.8 788,629 - metP OKGIIK_03555 0.61 -1.3 788,709 + metP OKGIIK_03555 0.73 -0.8 788,710 - metP OKGIIK_03555 0.73 -1.5 788,710 - metP OKGIIK_03555 0.73 -2.2 788,710 - metP OKGIIK_03555 0.73 -0.3 788,751 + metP OKGIIK_03555 0.79 -0.1 788,752 - metP OKGIIK_03555 0.79 -1.1 788,752 - metP OKGIIK_03555 0.79 +1.5 788,752 - metP OKGIIK_03555 0.79 -1.3 788,752 - metP OKGIIK_03555 0.79 -0.8 788,752 - metP OKGIIK_03555 0.79 -2.3 788,752 - metP OKGIIK_03555 0.79 +0.5 789,274 + abcC OKGIIK_03560 0.38 -1.3 789,274 + abcC OKGIIK_03560 0.38 -1.5 789,485 + abcC OKGIIK_03560 0.59 -3.9 789,486 - abcC OKGIIK_03560 0.59 -0.5 789,486 - abcC OKGIIK_03560 0.59 -0.5 789,486 - abcC OKGIIK_03560 0.59 -0.5 789,754 + abcC OKGIIK_03560 0.86 -1.7 789,754 + abcC OKGIIK_03560 0.86 -1.3 789,754 + abcC OKGIIK_03560 0.86 -3.7 789,755 - abcC OKGIIK_03560 0.86 -2.4 789,755 - abcC OKGIIK_03560 0.86 -1.8 789,867 - -1.0 789,867 - -2.1
Or see this region's nucleotide sequence