Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliQ and fliR overlap by 4 nucleotides fliR and flhB are separated by 5 nucleotides
OKGIIK_02800: fliQ - flagellar biosynthesis protein FliQ, at 645,626 to 645,895
fliQ
OKGIIK_02805: fliR - flagellar biosynthetic protein FliR, at 645,892 to 646,662
fliR
OKGIIK_02810: flhB - flagellar biosynthesis protein FlhB, at 646,668 to 647,804
flhB
Position (kb) 645
646
647 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 645.152 kb on + strand at 645.198 kb on - strand at 645.198 kb on - strand at 645.198 kb on - strand at 645.198 kb on - strand at 645.198 kb on - strand at 645.198 kb on - strand at 645.668 kb on + strand, within fliQ at 645.668 kb on + strand, within fliQ at 645.668 kb on + strand, within fliQ at 645.668 kb on + strand, within fliQ at 645.668 kb on + strand, within fliQ at 645.668 kb on + strand, within fliQ at 645.668 kb on + strand, within fliQ at 645.668 kb on + strand, within fliQ at 645.668 kb on + strand, within fliQ at 645.668 kb on + strand, within fliQ at 645.669 kb on - strand, within fliQ at 645.669 kb on - strand, within fliQ at 645.669 kb on - strand, within fliQ at 645.669 kb on - strand, within fliQ at 645.669 kb on - strand, within fliQ at 645.851 kb on + strand, within fliQ at 645.851 kb on + strand, within fliQ at 645.851 kb on + strand, within fliQ at 645.851 kb on + strand, within fliQ at 645.851 kb on + strand, within fliQ at 645.851 kb on + strand, within fliQ at 645.851 kb on + strand, within fliQ at 645.851 kb on + strand, within fliQ at 645.851 kb on + strand, within fliQ at 645.851 kb on + strand, within fliQ at 645.851 kb on + strand, within fliQ at 645.851 kb on + strand, within fliQ at 645.851 kb on + strand, within fliQ at 645.851 kb on + strand, within fliQ at 645.851 kb on + strand, within fliQ at 645.851 kb on + strand, within fliQ at 645.851 kb on + strand, within fliQ at 645.851 kb on + strand, within fliQ at 645.851 kb on + strand, within fliQ at 645.851 kb on + strand, within fliQ at 645.851 kb on + strand, within fliQ at 645.851 kb on + strand, within fliQ at 645.851 kb on + strand, within fliQ at 645.852 kb on - strand, within fliQ at 645.852 kb on - strand, within fliQ at 645.852 kb on - strand, within fliQ at 645.852 kb on - strand, within fliQ at 645.852 kb on - strand, within fliQ at 645.852 kb on - strand, within fliQ at 645.852 kb on - strand, within fliQ at 645.852 kb on - strand, within fliQ at 645.852 kb on - strand, within fliQ at 645.852 kb on - strand, within fliQ at 645.852 kb on - strand, within fliQ at 645.852 kb on - strand, within fliQ at 645.852 kb on - strand, within fliQ at 645.852 kb on - strand, within fliQ at 645.852 kb on - strand, within fliQ at 645.852 kb on - strand, within fliQ at 645.852 kb on - strand, within fliQ at 645.852 kb on - strand, within fliQ at 645.852 kb on - strand, within fliQ at 646.273 kb on + strand, within fliR at 646.273 kb on + strand, within fliR at 646.273 kb on + strand, within fliR at 646.273 kb on + strand, within fliR at 646.273 kb on + strand, within fliR at 646.273 kb on + strand, within fliR at 646.273 kb on + strand, within fliR at 646.273 kb on + strand, within fliR at 646.273 kb on + strand, within fliR at 646.273 kb on + strand, within fliR at 646.273 kb on + strand, within fliR at 646.273 kb on + strand, within fliR at 646.273 kb on + strand, within fliR at 646.273 kb on + strand, within fliR at 646.273 kb on + strand, within fliR at 646.273 kb on + strand, within fliR at 646.273 kb on + strand, within fliR at 646.273 kb on + strand, within fliR at 646.273 kb on + strand, within fliR at 646.273 kb on + strand, within fliR at 646.274 kb on - strand, within fliR at 646.274 kb on - strand, within fliR at 646.274 kb on - strand, within fliR at 646.274 kb on - strand, within fliR at 646.274 kb on - strand, within fliR at 646.274 kb on - strand, within fliR at 646.274 kb on - strand, within fliR at 646.274 kb on - strand, within fliR at 646.274 kb on - strand, within fliR at 646.706 kb on + strand at 646.707 kb on - strand at 646.866 kb on + strand, within flhB at 646.867 kb on - strand, within flhB at 646.867 kb on - strand, within flhB at 647.298 kb on + strand, within flhB at 647.298 kb on + strand, within flhB at 647.298 kb on + strand, within flhB at 647.298 kb on + strand, within flhB at 647.301 kb on - strand, within flhB at 647.503 kb on - strand, within flhB at 647.634 kb on + strand, within flhB at 647.634 kb on + strand, within flhB at 647.634 kb on + strand, within flhB at 647.634 kb on + strand, within flhB at 647.634 kb on + strand, within flhB at 647.634 kb on + strand, within flhB at 647.634 kb on + strand, within flhB at 647.634 kb on + strand, within flhB at 647.634 kb on + strand, within flhB at 647.634 kb on + strand, within flhB at 647.634 kb on + strand, within flhB at 647.634 kb on + strand, within flhB at 647.634 kb on + strand, within flhB at 647.634 kb on + strand, within flhB at 647.634 kb on + strand, within flhB at 647.634 kb on + strand, within flhB at 647.634 kb on + strand, within flhB at 647.634 kb on + strand, within flhB at 647.635 kb on - strand, within flhB at 647.635 kb on - strand, within flhB at 647.635 kb on - strand, within flhB at 647.635 kb on - strand, within flhB at 647.635 kb on - strand, within flhB at 647.635 kb on - strand, within flhB at 647.635 kb on - strand, within flhB at 647.635 kb on - strand, within flhB at 647.635 kb on - strand, within flhB at 647.635 kb on - strand, within flhB at 647.635 kb on - strand, within flhB at 647.635 kb on - strand, within flhB
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 645,152 + +2.1 645,198 - -1.7 645,198 - -1.1 645,198 - -1.0 645,198 - -2.1 645,198 - +0.5 645,198 - -1.8 645,668 + fliQ OKGIIK_02800 0.16 -1.5 645,668 + fliQ OKGIIK_02800 0.16 -0.1 645,668 + fliQ OKGIIK_02800 0.16 -1.5 645,668 + fliQ OKGIIK_02800 0.16 -1.2 645,668 + fliQ OKGIIK_02800 0.16 -0.1 645,668 + fliQ OKGIIK_02800 0.16 -1.0 645,668 + fliQ OKGIIK_02800 0.16 -0.3 645,668 + fliQ OKGIIK_02800 0.16 -0.5 645,668 + fliQ OKGIIK_02800 0.16 -3.0 645,668 + fliQ OKGIIK_02800 0.16 -1.1 645,669 - fliQ OKGIIK_02800 0.16 -1.1 645,669 - fliQ OKGIIK_02800 0.16 -1.3 645,669 - fliQ OKGIIK_02800 0.16 -0.0 645,669 - fliQ OKGIIK_02800 0.16 -0.1 645,669 - fliQ OKGIIK_02800 0.16 +0.2 645,851 + fliQ OKGIIK_02800 0.83 -1.1 645,851 + fliQ OKGIIK_02800 0.83 -1.7 645,851 + fliQ OKGIIK_02800 0.83 +0.2 645,851 + fliQ OKGIIK_02800 0.83 -1.1 645,851 + fliQ OKGIIK_02800 0.83 -0.8 645,851 + fliQ OKGIIK_02800 0.83 -2.2 645,851 + fliQ OKGIIK_02800 0.83 -0.3 645,851 + fliQ OKGIIK_02800 0.83 +0.9 645,851 + fliQ OKGIIK_02800 0.83 -0.3 645,851 + fliQ OKGIIK_02800 0.83 +0.6 645,851 + fliQ OKGIIK_02800 0.83 +1.2 645,851 + fliQ OKGIIK_02800 0.83 -1.3 645,851 + fliQ OKGIIK_02800 0.83 -0.3 645,851 + fliQ OKGIIK_02800 0.83 -2.3 645,851 + fliQ OKGIIK_02800 0.83 -1.3 645,851 + fliQ OKGIIK_02800 0.83 -1.1 645,851 + fliQ OKGIIK_02800 0.83 -3.0 645,851 + fliQ OKGIIK_02800 0.83 +1.3 645,851 + fliQ OKGIIK_02800 0.83 -0.5 645,851 + fliQ OKGIIK_02800 0.83 +2.1 645,851 + fliQ OKGIIK_02800 0.83 -1.1 645,851 + fliQ OKGIIK_02800 0.83 -3.6 645,851 + fliQ OKGIIK_02800 0.83 -0.8 645,852 - fliQ OKGIIK_02800 0.84 -1.7 645,852 - fliQ OKGIIK_02800 0.84 -2.3 645,852 - fliQ OKGIIK_02800 0.84 -1.3 645,852 - fliQ OKGIIK_02800 0.84 -1.1 645,852 - fliQ OKGIIK_02800 0.84 +0.5 645,852 - fliQ OKGIIK_02800 0.84 -1.7 645,852 - fliQ OKGIIK_02800 0.84 -0.7 645,852 - fliQ OKGIIK_02800 0.84 -1.3 645,852 - fliQ OKGIIK_02800 0.84 -2.1 645,852 - fliQ OKGIIK_02800 0.84 +0.5 645,852 - fliQ OKGIIK_02800 0.84 -1.3 645,852 - fliQ OKGIIK_02800 0.84 -1.5 645,852 - fliQ OKGIIK_02800 0.84 -0.1 645,852 - fliQ OKGIIK_02800 0.84 -1.3 645,852 - fliQ OKGIIK_02800 0.84 -1.7 645,852 - fliQ OKGIIK_02800 0.84 +0.7 645,852 - fliQ OKGIIK_02800 0.84 -0.5 645,852 - fliQ OKGIIK_02800 0.84 -1.7 645,852 - fliQ OKGIIK_02800 0.84 +0.5 646,273 + fliR OKGIIK_02805 0.49 -0.5 646,273 + fliR OKGIIK_02805 0.49 -2.5 646,273 + fliR OKGIIK_02805 0.49 -2.8 646,273 + fliR OKGIIK_02805 0.49 +0.5 646,273 + fliR OKGIIK_02805 0.49 +0.5 646,273 + fliR OKGIIK_02805 0.49 -1.8 646,273 + fliR OKGIIK_02805 0.49 -1.2 646,273 + fliR OKGIIK_02805 0.49 +0.5 646,273 + fliR OKGIIK_02805 0.49 -2.8 646,273 + fliR OKGIIK_02805 0.49 -1.1 646,273 + fliR OKGIIK_02805 0.49 -0.8 646,273 + fliR OKGIIK_02805 0.49 -2.0 646,273 + fliR OKGIIK_02805 0.49 -0.8 646,273 + fliR OKGIIK_02805 0.49 -0.1 646,273 + fliR OKGIIK_02805 0.49 -2.1 646,273 + fliR OKGIIK_02805 0.49 -1.3 646,273 + fliR OKGIIK_02805 0.49 +2.1 646,273 + fliR OKGIIK_02805 0.49 -0.8 646,273 + fliR OKGIIK_02805 0.49 -0.3 646,273 + fliR OKGIIK_02805 0.49 -2.4 646,274 - fliR OKGIIK_02805 0.50 -1.4 646,274 - fliR OKGIIK_02805 0.50 -1.5 646,274 - fliR OKGIIK_02805 0.50 -1.3 646,274 - fliR OKGIIK_02805 0.50 -0.8 646,274 - fliR OKGIIK_02805 0.50 -1.5 646,274 - fliR OKGIIK_02805 0.50 -0.3 646,274 - fliR OKGIIK_02805 0.50 -1.7 646,274 - fliR OKGIIK_02805 0.50 -0.5 646,274 - fliR OKGIIK_02805 0.50 -1.7 646,706 + +0.5 646,707 - -0.6 646,866 + flhB OKGIIK_02810 0.17 +1.5 646,867 - flhB OKGIIK_02810 0.18 -0.4 646,867 - flhB OKGIIK_02810 0.18 -0.1 647,298 + flhB OKGIIK_02810 0.55 -1.1 647,298 + flhB OKGIIK_02810 0.55 -3.8 647,298 + flhB OKGIIK_02810 0.55 +0.7 647,298 + flhB OKGIIK_02810 0.55 -0.4 647,301 - flhB OKGIIK_02810 0.56 -0.7 647,503 - flhB OKGIIK_02810 0.73 -3.4 647,634 + flhB OKGIIK_02810 0.85 -1.3 647,634 + flhB OKGIIK_02810 0.85 -0.1 647,634 + flhB OKGIIK_02810 0.85 -3.6 647,634 + flhB OKGIIK_02810 0.85 -0.7 647,634 + flhB OKGIIK_02810 0.85 -0.9 647,634 + flhB OKGIIK_02810 0.85 -2.4 647,634 + flhB OKGIIK_02810 0.85 -2.5 647,634 + flhB OKGIIK_02810 0.85 -1.1 647,634 + flhB OKGIIK_02810 0.85 +1.5 647,634 + flhB OKGIIK_02810 0.85 +0.5 647,634 + flhB OKGIIK_02810 0.85 -1.8 647,634 + flhB OKGIIK_02810 0.85 -1.1 647,634 + flhB OKGIIK_02810 0.85 -2.1 647,634 + flhB OKGIIK_02810 0.85 +1.3 647,634 + flhB OKGIIK_02810 0.85 +1.5 647,634 + flhB OKGIIK_02810 0.85 -0.1 647,634 + flhB OKGIIK_02810 0.85 -3.0 647,634 + flhB OKGIIK_02810 0.85 -0.3 647,635 - flhB OKGIIK_02810 0.85 -1.5 647,635 - flhB OKGIIK_02810 0.85 +3.8 647,635 - flhB OKGIIK_02810 0.85 -0.6 647,635 - flhB OKGIIK_02810 0.85 -2.4 647,635 - flhB OKGIIK_02810 0.85 -0.1 647,635 - flhB OKGIIK_02810 0.85 -0.7 647,635 - flhB OKGIIK_02810 0.85 -1.1 647,635 - flhB OKGIIK_02810 0.85 +1.5 647,635 - flhB OKGIIK_02810 0.85 -2.4 647,635 - flhB OKGIIK_02810 0.85 -2.0 647,635 - flhB OKGIIK_02810 0.85 +0.5 647,635 - flhB OKGIIK_02810 0.85 -0.8
Or see this region's nucleotide sequence