Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_01770 and OKGIIK_01775 are separated by 205 nucleotides OKGIIK_01775 and OKGIIK_01780 are separated by 65 nucleotides OKGIIK_01780 and OKGIIK_01785 are separated by 106 nucleotides
OKGIIK_01770: OKGIIK_01770 - Transcriptional regulator ArgP, at 409,908 to 410,813
_01770
OKGIIK_01775: OKGIIK_01775 - L-lysine exporter family protein LysE/ArgO, at 411,019 to 411,636
_01775
OKGIIK_01780: OKGIIK_01780 - NAD+ synthase, at 411,702 to 413,387
_01780
OKGIIK_01785: OKGIIK_01785 - Outer membrane protein assembly factor BamD, at 413,494 to 414,360
_01785
Position (kb) 411
412
413
414 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 410.932 kb on + strand at 410.951 kb on + strand at 410.951 kb on + strand at 410.951 kb on + strand at 410.951 kb on + strand at 410.951 kb on + strand at 410.952 kb on - strand at 410.952 kb on - strand at 410.957 kb on + strand at 410.958 kb on - strand at 410.958 kb on - strand at 410.961 kb on + strand at 410.962 kb on - strand at 410.962 kb on - strand at 411.024 kb on + strand at 411.024 kb on + strand at 411.024 kb on + strand at 411.024 kb on + strand at 411.024 kb on + strand at 411.024 kb on + strand at 411.024 kb on + strand at 411.024 kb on + strand at 411.024 kb on + strand at 411.024 kb on + strand at 411.024 kb on + strand at 411.025 kb on - strand at 411.025 kb on - strand at 411.025 kb on - strand at 411.025 kb on - strand at 411.025 kb on - strand at 411.025 kb on - strand at 411.025 kb on - strand at 411.034 kb on + strand at 411.034 kb on + strand at 411.034 kb on + strand at 411.034 kb on + strand at 411.035 kb on - strand at 411.035 kb on - strand at 411.035 kb on - strand at 411.035 kb on - strand at 411.076 kb on - strand at 411.076 kb on - strand at 411.076 kb on - strand at 411.241 kb on + strand, within OKGIIK_01775 at 411.241 kb on + strand, within OKGIIK_01775 at 411.242 kb on - strand, within OKGIIK_01775 at 411.242 kb on - strand, within OKGIIK_01775 at 411.242 kb on - strand, within OKGIIK_01775 at 411.242 kb on - strand, within OKGIIK_01775 at 411.242 kb on - strand, within OKGIIK_01775 at 411.269 kb on - strand, within OKGIIK_01775 at 411.269 kb on - strand, within OKGIIK_01775 at 411.395 kb on + strand, within OKGIIK_01775 at 411.397 kb on + strand, within OKGIIK_01775 at 411.397 kb on + strand, within OKGIIK_01775 at 411.397 kb on + strand, within OKGIIK_01775 at 411.397 kb on + strand, within OKGIIK_01775 at 411.397 kb on + strand, within OKGIIK_01775 at 411.397 kb on + strand, within OKGIIK_01775 at 411.397 kb on + strand, within OKGIIK_01775 at 411.397 kb on + strand, within OKGIIK_01775 at 411.397 kb on + strand, within OKGIIK_01775 at 411.397 kb on + strand, within OKGIIK_01775 at 411.397 kb on + strand, within OKGIIK_01775 at 411.398 kb on - strand, within OKGIIK_01775 at 411.398 kb on - strand, within OKGIIK_01775 at 411.398 kb on - strand, within OKGIIK_01775 at 411.398 kb on - strand, within OKGIIK_01775 at 411.398 kb on - strand, within OKGIIK_01775 at 411.398 kb on - strand, within OKGIIK_01775 at 411.398 kb on - strand, within OKGIIK_01775 at 411.398 kb on - strand, within OKGIIK_01775 at 411.398 kb on - strand, within OKGIIK_01775 at 411.398 kb on - strand, within OKGIIK_01775 at 411.439 kb on + strand, within OKGIIK_01775 at 411.439 kb on + strand, within OKGIIK_01775 at 411.439 kb on + strand, within OKGIIK_01775 at 411.440 kb on - strand, within OKGIIK_01775 at 411.440 kb on - strand, within OKGIIK_01775 at 411.514 kb on + strand, within OKGIIK_01775 at 411.514 kb on + strand, within OKGIIK_01775 at 411.514 kb on + strand, within OKGIIK_01775 at 411.514 kb on + strand, within OKGIIK_01775 at 411.514 kb on + strand, within OKGIIK_01775 at 411.515 kb on - strand, within OKGIIK_01775 at 411.515 kb on - strand, within OKGIIK_01775 at 411.515 kb on - strand, within OKGIIK_01775 at 411.515 kb on - strand, within OKGIIK_01775 at 411.515 kb on - strand, within OKGIIK_01775 at 411.665 kb on - strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.669 kb on + strand at 411.670 kb on - strand at 411.670 kb on - strand at 411.670 kb on - strand at 411.670 kb on - strand at 411.677 kb on + strand at 411.677 kb on + strand at 411.678 kb on - strand at 411.707 kb on + strand at 411.713 kb on - strand at 411.713 kb on - strand at 411.850 kb on - strand at 412.047 kb on + strand, within OKGIIK_01780 at 412.297 kb on - strand, within OKGIIK_01780 at 412.297 kb on - strand, within OKGIIK_01780 at 412.332 kb on + strand, within OKGIIK_01780 at 412.332 kb on + strand, within OKGIIK_01780 at 412.333 kb on - strand, within OKGIIK_01780 at 412.494 kb on + strand, within OKGIIK_01780 at 412.494 kb on + strand, within OKGIIK_01780 at 412.494 kb on + strand, within OKGIIK_01780 at 412.495 kb on - strand, within OKGIIK_01780 at 412.495 kb on - strand, within OKGIIK_01780 at 412.495 kb on - strand, within OKGIIK_01780 at 412.524 kb on + strand, within OKGIIK_01780 at 412.524 kb on + strand, within OKGIIK_01780 at 412.524 kb on + strand, within OKGIIK_01780 at 412.524 kb on + strand, within OKGIIK_01780 at 412.524 kb on + strand, within OKGIIK_01780 at 412.525 kb on - strand, within OKGIIK_01780 at 412.525 kb on - strand, within OKGIIK_01780 at 412.525 kb on - strand, within OKGIIK_01780 at 412.525 kb on - strand, within OKGIIK_01780 at 412.656 kb on + strand, within OKGIIK_01780 at 412.657 kb on - strand, within OKGIIK_01780 at 412.657 kb on - strand, within OKGIIK_01780 at 412.716 kb on + strand, within OKGIIK_01780 at 412.912 kb on - strand, within OKGIIK_01780 at 412.923 kb on + strand, within OKGIIK_01780 at 412.945 kb on - strand, within OKGIIK_01780 at 412.945 kb on - strand, within OKGIIK_01780 at 412.965 kb on + strand, within OKGIIK_01780 at 412.966 kb on - strand, within OKGIIK_01780 at 412.966 kb on - strand, within OKGIIK_01780 at 412.966 kb on - strand, within OKGIIK_01780 at 412.966 kb on - strand, within OKGIIK_01780 at 413.169 kb on + strand, within OKGIIK_01780 at 413.369 kb on + strand at 413.369 kb on + strand at 413.369 kb on + strand at 413.370 kb on - strand at 413.370 kb on - strand at 413.370 kb on - strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.444 kb on + strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand at 413.445 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 410,932 + -1.8 410,951 + -1.3 410,951 + -1.8 410,951 + -1.3 410,951 + -2.4 410,951 + -0.5 410,952 - -1.4 410,952 - +0.1 410,957 + -1.1 410,958 - +1.2 410,958 - -0.1 410,961 + +0.5 410,962 - -1.1 410,962 - -0.1 411,024 + -1.3 411,024 + -1.7 411,024 + -1.7 411,024 + -1.7 411,024 + +2.3 411,024 + -1.5 411,024 + +0.6 411,024 + -1.5 411,024 + +1.5 411,024 + -1.9 411,024 + -1.6 411,025 - -0.6 411,025 - -1.3 411,025 - +0.5 411,025 - -0.1 411,025 - +1.5 411,025 - +0.1 411,025 - -1.8 411,034 + -1.6 411,034 + -1.3 411,034 + -2.4 411,034 + -1.2 411,035 - -0.5 411,035 - -2.1 411,035 - -0.1 411,035 - -2.1 411,076 - +1.5 411,076 - -1.7 411,076 - -1.3 411,241 + OKGIIK_01775 0.36 +1.5 411,241 + OKGIIK_01775 0.36 -1.2 411,242 - OKGIIK_01775 0.36 -2.5 411,242 - OKGIIK_01775 0.36 +2.1 411,242 - OKGIIK_01775 0.36 +0.5 411,242 - OKGIIK_01775 0.36 -2.2 411,242 - OKGIIK_01775 0.36 -2.9 411,269 - OKGIIK_01775 0.40 -3.2 411,269 - OKGIIK_01775 0.40 -0.5 411,395 + OKGIIK_01775 0.61 +0.9 411,397 + OKGIIK_01775 0.61 +0.5 411,397 + OKGIIK_01775 0.61 -1.5 411,397 + OKGIIK_01775 0.61 -1.4 411,397 + OKGIIK_01775 0.61 -0.7 411,397 + OKGIIK_01775 0.61 -0.5 411,397 + OKGIIK_01775 0.61 +1.5 411,397 + OKGIIK_01775 0.61 -1.5 411,397 + OKGIIK_01775 0.61 -1.1 411,397 + OKGIIK_01775 0.61 -1.4 411,397 + OKGIIK_01775 0.61 -1.0 411,397 + OKGIIK_01775 0.61 -1.5 411,398 - OKGIIK_01775 0.61 -1.7 411,398 - OKGIIK_01775 0.61 -2.2 411,398 - OKGIIK_01775 0.61 -0.6 411,398 - OKGIIK_01775 0.61 -1.6 411,398 - OKGIIK_01775 0.61 -0.1 411,398 - OKGIIK_01775 0.61 -1.6 411,398 - OKGIIK_01775 0.61 -1.5 411,398 - OKGIIK_01775 0.61 +1.2 411,398 - OKGIIK_01775 0.61 -1.1 411,398 - OKGIIK_01775 0.61 -2.5 411,439 + OKGIIK_01775 0.68 -1.5 411,439 + OKGIIK_01775 0.68 -0.5 411,439 + OKGIIK_01775 0.68 -2.1 411,440 - OKGIIK_01775 0.68 +0.5 411,440 - OKGIIK_01775 0.68 +0.5 411,514 + OKGIIK_01775 0.80 +0.9 411,514 + OKGIIK_01775 0.80 +0.5 411,514 + OKGIIK_01775 0.80 -1.5 411,514 + OKGIIK_01775 0.80 +0.4 411,514 + OKGIIK_01775 0.80 -1.6 411,515 - OKGIIK_01775 0.80 -1.5 411,515 - OKGIIK_01775 0.80 -0.8 411,515 - OKGIIK_01775 0.80 -1.5 411,515 - OKGIIK_01775 0.80 -0.6 411,515 - OKGIIK_01775 0.80 -2.2 411,665 - -1.1 411,669 + -1.3 411,669 + -2.3 411,669 + -0.1 411,669 + +0.1 411,669 + -1.8 411,669 + +1.2 411,669 + +0.1 411,669 + -0.1 411,669 + -1.6 411,669 + +1.5 411,669 + -1.5 411,669 + -1.1 411,669 + +0.2 411,669 + -0.1 411,669 + -2.4 411,669 + -2.1 411,669 + -0.5 411,670 - -0.5 411,670 - -1.3 411,670 - -0.5 411,670 - +0.5 411,677 + -0.5 411,677 + +0.5 411,678 - +0.2 411,707 + -0.8 411,713 - +1.5 411,713 - -1.3 411,850 - -0.1 412,047 + OKGIIK_01780 0.20 +0.5 412,297 - OKGIIK_01780 0.35 -0.1 412,297 - OKGIIK_01780 0.35 -1.3 412,332 + OKGIIK_01780 0.37 +1.5 412,332 + OKGIIK_01780 0.37 -0.8 412,333 - OKGIIK_01780 0.37 +0.5 412,494 + OKGIIK_01780 0.47 -1.1 412,494 + OKGIIK_01780 0.47 +0.4 412,494 + OKGIIK_01780 0.47 +0.5 412,495 - OKGIIK_01780 0.47 +0.5 412,495 - OKGIIK_01780 0.47 +0.5 412,495 - OKGIIK_01780 0.47 -1.1 412,524 + OKGIIK_01780 0.49 -2.3 412,524 + OKGIIK_01780 0.49 -0.5 412,524 + OKGIIK_01780 0.49 -1.1 412,524 + OKGIIK_01780 0.49 -0.1 412,524 + OKGIIK_01780 0.49 -2.6 412,525 - OKGIIK_01780 0.49 -2.5 412,525 - OKGIIK_01780 0.49 -1.3 412,525 - OKGIIK_01780 0.49 -3.0 412,525 - OKGIIK_01780 0.49 +0.5 412,656 + OKGIIK_01780 0.57 +0.5 412,657 - OKGIIK_01780 0.57 -0.5 412,657 - OKGIIK_01780 0.57 +1.5 412,716 + OKGIIK_01780 0.60 -1.3 412,912 - OKGIIK_01780 0.72 -0.5 412,923 + OKGIIK_01780 0.72 +1.5 412,945 - OKGIIK_01780 0.74 +0.5 412,945 - OKGIIK_01780 0.74 -1.1 412,965 + OKGIIK_01780 0.75 -2.5 412,966 - OKGIIK_01780 0.75 -0.8 412,966 - OKGIIK_01780 0.75 -0.1 412,966 - OKGIIK_01780 0.75 -1.1 412,966 - OKGIIK_01780 0.75 -0.1 413,169 + OKGIIK_01780 0.87 +0.5 413,369 + +0.5 413,369 + -0.6 413,369 + +0.5 413,370 - +1.5 413,370 - -1.1 413,370 - -0.8 413,444 + -0.8 413,444 + +0.5 413,444 + -1.1 413,444 + +2.1 413,444 + -0.1 413,444 + -2.6 413,444 + -1.3 413,444 + -0.6 413,444 + -0.5 413,444 + +0.5 413,444 + +0.1 413,444 + -0.4 413,444 + -1.8 413,444 + -1.6 413,444 + +0.4 413,445 - +1.3 413,445 - +1.2 413,445 - -0.5 413,445 - -0.2 413,445 - -1.9 413,445 - -2.3 413,445 - -1.1 413,445 - +1.1 413,445 - -0.6 413,445 - -0.8 413,445 - -1.9 413,445 - -1.8 413,445 - -1.5 413,445 - -1.9 413,445 - -1.5 413,445 - -0.1 413,445 - -0.5 413,445 - +1.5 413,445 - -0.3
Or see this region's nucleotide sequence