Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sdaA and OKGIIK_01570 are separated by 77 nucleotides OKGIIK_01570 and OKGIIK_01575 are separated by 225 nucleotides OKGIIK_01575 and OKGIIK_01580 are separated by 95 nucleotides
OKGIIK_01565: sdaA - L-serine ammonia-lyase, at 356,162 to 357,544
sdaA
OKGIIK_01570: OKGIIK_01570 - NrdH-redoxin, at 357,622 to 357,852
_01570
OKGIIK_01575: OKGIIK_01575 - hypothetical protein, at 358,078 to 358,650
_01575
OKGIIK_01580: OKGIIK_01580 - GTP-binding protein HSR1, at 358,746 to 360,110
_01580
Position (kb) 357
358 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 356.628 kb on + strand, within sdaA at 356.628 kb on + strand, within sdaA at 356.628 kb on + strand, within sdaA at 356.628 kb on + strand, within sdaA at 356.628 kb on + strand, within sdaA at 356.628 kb on + strand, within sdaA at 356.629 kb on - strand, within sdaA at 356.629 kb on - strand, within sdaA at 356.629 kb on - strand, within sdaA at 356.629 kb on - strand, within sdaA at 356.629 kb on - strand, within sdaA at 356.631 kb on + strand, within sdaA at 356.631 kb on + strand, within sdaA at 356.632 kb on - strand, within sdaA at 356.632 kb on - strand, within sdaA at 356.632 kb on - strand, within sdaA at 356.632 kb on - strand, within sdaA at 356.632 kb on - strand, within sdaA at 356.632 kb on - strand, within sdaA at 356.727 kb on + strand, within sdaA at 356.727 kb on + strand, within sdaA at 356.727 kb on + strand, within sdaA at 356.728 kb on - strand, within sdaA at 356.728 kb on - strand, within sdaA at 356.728 kb on - strand, within sdaA at 356.728 kb on - strand, within sdaA at 357.024 kb on + strand, within sdaA at 357.024 kb on + strand, within sdaA at 357.024 kb on + strand, within sdaA at 357.025 kb on - strand, within sdaA at 357.025 kb on - strand, within sdaA at 357.025 kb on - strand, within sdaA at 357.025 kb on - strand, within sdaA at 357.105 kb on + strand, within sdaA at 357.105 kb on + strand, within sdaA at 357.105 kb on + strand, within sdaA at 357.105 kb on + strand, within sdaA at 357.105 kb on + strand, within sdaA at 357.106 kb on - strand, within sdaA at 357.106 kb on - strand, within sdaA at 357.106 kb on - strand, within sdaA at 357.106 kb on - strand, within sdaA at 357.108 kb on + strand, within sdaA at 357.108 kb on + strand, within sdaA at 357.108 kb on + strand, within sdaA at 357.109 kb on - strand, within sdaA at 357.109 kb on - strand, within sdaA at 357.109 kb on - strand, within sdaA at 357.109 kb on - strand, within sdaA at 357.109 kb on - strand, within sdaA at 357.391 kb on - strand, within sdaA at 357.391 kb on - strand, within sdaA at 357.391 kb on - strand, within sdaA at 357.391 kb on - strand, within sdaA at 357.530 kb on + strand at 357.531 kb on - strand at 357.531 kb on - strand at 357.578 kb on - strand at 357.582 kb on + strand at 357.582 kb on + strand at 357.582 kb on + strand at 357.583 kb on - strand at 357.583 kb on - strand at 357.583 kb on - strand at 357.583 kb on - strand at 357.640 kb on + strand at 357.641 kb on - strand at 357.652 kb on + strand, within OKGIIK_01570 at 357.652 kb on + strand, within OKGIIK_01570 at 357.653 kb on - strand, within OKGIIK_01570 at 357.653 kb on - strand, within OKGIIK_01570 at 357.653 kb on - strand, within OKGIIK_01570 at 357.754 kb on + strand, within OKGIIK_01570 at 357.754 kb on + strand, within OKGIIK_01570 at 357.850 kb on + strand at 357.850 kb on + strand at 357.850 kb on + strand at 357.850 kb on + strand at 357.850 kb on + strand at 357.850 kb on + strand at 357.850 kb on + strand at 357.850 kb on + strand at 357.850 kb on + strand at 357.850 kb on + strand at 357.850 kb on + strand at 357.850 kb on + strand at 357.850 kb on + strand at 357.850 kb on + strand at 357.850 kb on + strand at 357.851 kb on - strand at 357.851 kb on - strand at 357.851 kb on - strand at 357.851 kb on - strand at 357.851 kb on - strand at 357.851 kb on - strand at 357.851 kb on - strand at 357.851 kb on - strand at 357.851 kb on - strand at 357.851 kb on - strand at 357.851 kb on - strand at 357.851 kb on - strand at 357.851 kb on - strand at 357.859 kb on + strand at 358.091 kb on + strand at 358.091 kb on + strand at 358.091 kb on + strand at 358.091 kb on + strand at 358.091 kb on + strand at 358.092 kb on - strand at 358.092 kb on - strand at 358.328 kb on + strand, within OKGIIK_01575 at 358.328 kb on + strand, within OKGIIK_01575 at 358.329 kb on - strand, within OKGIIK_01575 at 358.329 kb on - strand, within OKGIIK_01575 at 358.329 kb on - strand, within OKGIIK_01575 at 358.424 kb on + strand, within OKGIIK_01575 at 358.424 kb on + strand, within OKGIIK_01575 at 358.424 kb on + strand, within OKGIIK_01575 at 358.425 kb on - strand, within OKGIIK_01575 at 358.425 kb on - strand, within OKGIIK_01575 at 358.425 kb on - strand, within OKGIIK_01575 at 358.425 kb on - strand, within OKGIIK_01575 at 358.425 kb on - strand, within OKGIIK_01575 at 358.427 kb on + strand, within OKGIIK_01575 at 358.427 kb on + strand, within OKGIIK_01575 at 358.427 kb on + strand, within OKGIIK_01575 at 358.427 kb on + strand, within OKGIIK_01575 at 358.427 kb on + strand, within OKGIIK_01575 at 358.427 kb on + strand, within OKGIIK_01575 at 358.427 kb on + strand, within OKGIIK_01575 at 358.427 kb on + strand, within OKGIIK_01575 at 358.427 kb on + strand, within OKGIIK_01575 at 358.427 kb on + strand, within OKGIIK_01575 at 358.427 kb on + strand, within OKGIIK_01575 at 358.427 kb on + strand, within OKGIIK_01575 at 358.427 kb on + strand, within OKGIIK_01575 at 358.428 kb on - strand, within OKGIIK_01575 at 358.428 kb on - strand, within OKGIIK_01575 at 358.428 kb on - strand, within OKGIIK_01575 at 358.428 kb on - strand, within OKGIIK_01575 at 358.428 kb on - strand, within OKGIIK_01575 at 358.428 kb on - strand, within OKGIIK_01575 at 358.428 kb on - strand, within OKGIIK_01575 at 358.428 kb on - strand, within OKGIIK_01575 at 358.428 kb on - strand, within OKGIIK_01575 at 358.428 kb on - strand, within OKGIIK_01575 at 358.428 kb on - strand, within OKGIIK_01575 at 358.428 kb on - strand, within OKGIIK_01575 at 358.428 kb on - strand, within OKGIIK_01575 at 358.428 kb on - strand, within OKGIIK_01575 at 358.428 kb on - strand, within OKGIIK_01575 at 358.428 kb on - strand, within OKGIIK_01575 at 358.428 kb on - strand, within OKGIIK_01575 at 358.428 kb on - strand, within OKGIIK_01575 at 358.428 kb on - strand, within OKGIIK_01575 at 358.428 kb on - strand, within OKGIIK_01575 at 358.428 kb on - strand, within OKGIIK_01575 at 358.428 kb on - strand, within OKGIIK_01575 at 358.428 kb on - strand, within OKGIIK_01575 at 358.428 kb on - strand, within OKGIIK_01575 at 358.428 kb on - strand, within OKGIIK_01575 at 358.514 kb on + strand, within OKGIIK_01575 at 358.514 kb on + strand, within OKGIIK_01575 at 358.514 kb on + strand, within OKGIIK_01575 at 358.514 kb on + strand, within OKGIIK_01575 at 358.514 kb on + strand, within OKGIIK_01575 at 358.514 kb on + strand, within OKGIIK_01575 at 358.514 kb on + strand, within OKGIIK_01575 at 358.514 kb on + strand, within OKGIIK_01575 at 358.514 kb on + strand, within OKGIIK_01575 at 358.514 kb on + strand, within OKGIIK_01575 at 358.514 kb on + strand, within OKGIIK_01575 at 358.515 kb on - strand, within OKGIIK_01575 at 358.515 kb on - strand, within OKGIIK_01575 at 358.515 kb on - strand, within OKGIIK_01575 at 358.515 kb on - strand, within OKGIIK_01575 at 358.515 kb on - strand, within OKGIIK_01575 at 358.515 kb on - strand, within OKGIIK_01575 at 358.515 kb on - strand, within OKGIIK_01575 at 358.515 kb on - strand, within OKGIIK_01575 at 358.515 kb on - strand, within OKGIIK_01575 at 358.515 kb on - strand, within OKGIIK_01575 at 358.515 kb on - strand, within OKGIIK_01575 at 358.515 kb on - strand, within OKGIIK_01575 at 358.689 kb on + strand at 358.689 kb on + strand at 358.715 kb on + strand at 358.716 kb on - strand at 358.716 kb on - strand at 358.729 kb on + strand at 358.729 kb on + strand at 358.729 kb on + strand at 358.729 kb on + strand at 358.730 kb on - strand at 358.730 kb on - strand at 358.730 kb on - strand at 358.730 kb on - strand at 358.730 kb on - strand at 358.730 kb on - strand at 358.730 kb on - strand at 358.739 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 356,628 + sdaA OKGIIK_01565 0.34 -0.8 356,628 + sdaA OKGIIK_01565 0.34 -0.2 356,628 + sdaA OKGIIK_01565 0.34 -1.3 356,628 + sdaA OKGIIK_01565 0.34 -0.5 356,628 + sdaA OKGIIK_01565 0.34 -1.6 356,628 + sdaA OKGIIK_01565 0.34 -0.5 356,629 - sdaA OKGIIK_01565 0.34 -0.0 356,629 - sdaA OKGIIK_01565 0.34 -1.0 356,629 - sdaA OKGIIK_01565 0.34 +1.2 356,629 - sdaA OKGIIK_01565 0.34 +0.5 356,629 - sdaA OKGIIK_01565 0.34 -1.5 356,631 + sdaA OKGIIK_01565 0.34 -1.6 356,631 + sdaA OKGIIK_01565 0.34 -1.6 356,632 - sdaA OKGIIK_01565 0.34 -1.0 356,632 - sdaA OKGIIK_01565 0.34 +0.2 356,632 - sdaA OKGIIK_01565 0.34 -0.8 356,632 - sdaA OKGIIK_01565 0.34 -1.2 356,632 - sdaA OKGIIK_01565 0.34 +0.5 356,632 - sdaA OKGIIK_01565 0.34 -3.1 356,727 + sdaA OKGIIK_01565 0.41 -1.9 356,727 + sdaA OKGIIK_01565 0.41 -1.6 356,727 + sdaA OKGIIK_01565 0.41 +1.5 356,728 - sdaA OKGIIK_01565 0.41 -1.0 356,728 - sdaA OKGIIK_01565 0.41 -1.5 356,728 - sdaA OKGIIK_01565 0.41 -1.2 356,728 - sdaA OKGIIK_01565 0.41 -2.5 357,024 + sdaA OKGIIK_01565 0.62 -0.5 357,024 + sdaA OKGIIK_01565 0.62 -0.8 357,024 + sdaA OKGIIK_01565 0.62 -0.0 357,025 - sdaA OKGIIK_01565 0.62 +0.5 357,025 - sdaA OKGIIK_01565 0.62 -1.3 357,025 - sdaA OKGIIK_01565 0.62 -0.9 357,025 - sdaA OKGIIK_01565 0.62 -1.8 357,105 + sdaA OKGIIK_01565 0.68 -1.6 357,105 + sdaA OKGIIK_01565 0.68 -2.3 357,105 + sdaA OKGIIK_01565 0.68 -1.0 357,105 + sdaA OKGIIK_01565 0.68 -2.0 357,105 + sdaA OKGIIK_01565 0.68 -0.0 357,106 - sdaA OKGIIK_01565 0.68 -1.3 357,106 - sdaA OKGIIK_01565 0.68 -1.3 357,106 - sdaA OKGIIK_01565 0.68 -0.0 357,106 - sdaA OKGIIK_01565 0.68 -1.9 357,108 + sdaA OKGIIK_01565 0.68 -0.9 357,108 + sdaA OKGIIK_01565 0.68 +0.2 357,108 + sdaA OKGIIK_01565 0.68 -1.8 357,109 - sdaA OKGIIK_01565 0.68 -1.5 357,109 - sdaA OKGIIK_01565 0.68 -2.6 357,109 - sdaA OKGIIK_01565 0.68 -1.0 357,109 - sdaA OKGIIK_01565 0.68 -0.0 357,109 - sdaA OKGIIK_01565 0.68 -1.0 357,391 - sdaA OKGIIK_01565 0.89 -2.0 357,391 - sdaA OKGIIK_01565 0.89 -2.2 357,391 - sdaA OKGIIK_01565 0.89 -0.0 357,391 - sdaA OKGIIK_01565 0.89 -1.3 357,530 + -2.4 357,531 - +0.5 357,531 - -0.5 357,578 - -0.0 357,582 + -1.0 357,582 + +1.5 357,582 + +0.2 357,583 - +0.7 357,583 - -0.2 357,583 - -2.5 357,583 - -1.3 357,640 + +1.5 357,641 - -1.0 357,652 + OKGIIK_01570 0.13 -2.3 357,652 + OKGIIK_01570 0.13 +0.5 357,653 - OKGIIK_01570 0.13 -0.0 357,653 - OKGIIK_01570 0.13 +1.5 357,653 - OKGIIK_01570 0.13 -1.5 357,754 + OKGIIK_01570 0.57 -2.2 357,754 + OKGIIK_01570 0.57 -1.0 357,850 + +0.5 357,850 + -1.5 357,850 + -0.8 357,850 + -1.5 357,850 + -1.5 357,850 + -1.3 357,850 + -1.5 357,850 + -1.0 357,850 + -1.0 357,850 + +0.5 357,850 + +1.5 357,850 + -1.9 357,850 + +0.1 357,850 + -0.8 357,850 + +0.0 357,851 - -0.9 357,851 - +0.1 357,851 - -0.5 357,851 - -1.1 357,851 - -0.6 357,851 - -0.5 357,851 - -0.2 357,851 - +2.5 357,851 - -0.7 357,851 - -1.6 357,851 - -1.0 357,851 - -0.0 357,851 - -0.6 357,859 + +0.1 358,091 + -1.7 358,091 + -2.5 358,091 + -0.3 358,091 + -0.5 358,091 + -1.6 358,092 - -1.8 358,092 - -0.4 358,328 + OKGIIK_01575 0.44 -0.8 358,328 + OKGIIK_01575 0.44 -0.9 358,329 - OKGIIK_01575 0.44 -1.9 358,329 - OKGIIK_01575 0.44 -0.5 358,329 - OKGIIK_01575 0.44 -0.8 358,424 + OKGIIK_01575 0.60 -3.0 358,424 + OKGIIK_01575 0.60 -1.8 358,424 + OKGIIK_01575 0.60 -1.6 358,425 - OKGIIK_01575 0.61 -1.6 358,425 - OKGIIK_01575 0.61 -1.5 358,425 - OKGIIK_01575 0.61 -1.8 358,425 - OKGIIK_01575 0.61 -0.2 358,425 - OKGIIK_01575 0.61 -2.0 358,427 + OKGIIK_01575 0.61 -1.3 358,427 + OKGIIK_01575 0.61 +1.1 358,427 + OKGIIK_01575 0.61 -2.3 358,427 + OKGIIK_01575 0.61 -3.4 358,427 + OKGIIK_01575 0.61 +1.5 358,427 + OKGIIK_01575 0.61 -0.7 358,427 + OKGIIK_01575 0.61 -0.8 358,427 + OKGIIK_01575 0.61 +0.5 358,427 + OKGIIK_01575 0.61 +0.5 358,427 + OKGIIK_01575 0.61 +1.2 358,427 + OKGIIK_01575 0.61 -1.5 358,427 + OKGIIK_01575 0.61 -2.2 358,427 + OKGIIK_01575 0.61 -0.5 358,428 - OKGIIK_01575 0.61 +0.8 358,428 - OKGIIK_01575 0.61 -1.9 358,428 - OKGIIK_01575 0.61 +0.2 358,428 - OKGIIK_01575 0.61 -0.5 358,428 - OKGIIK_01575 0.61 -0.5 358,428 - OKGIIK_01575 0.61 -0.6 358,428 - OKGIIK_01575 0.61 -0.3 358,428 - OKGIIK_01575 0.61 -2.8 358,428 - OKGIIK_01575 0.61 +0.5 358,428 - OKGIIK_01575 0.61 +0.3 358,428 - OKGIIK_01575 0.61 -1.5 358,428 - OKGIIK_01575 0.61 -0.8 358,428 - OKGIIK_01575 0.61 -0.5 358,428 - OKGIIK_01575 0.61 -1.6 358,428 - OKGIIK_01575 0.61 -0.1 358,428 - OKGIIK_01575 0.61 -2.6 358,428 - OKGIIK_01575 0.61 -1.5 358,428 - OKGIIK_01575 0.61 -1.3 358,428 - OKGIIK_01575 0.61 +1.5 358,428 - OKGIIK_01575 0.61 -2.2 358,428 - OKGIIK_01575 0.61 +4.5 358,428 - OKGIIK_01575 0.61 -0.3 358,428 - OKGIIK_01575 0.61 -0.8 358,428 - OKGIIK_01575 0.61 -0.5 358,428 - OKGIIK_01575 0.61 -0.8 358,514 + OKGIIK_01575 0.76 +1.5 358,514 + OKGIIK_01575 0.76 -0.3 358,514 + OKGIIK_01575 0.76 +1.5 358,514 + OKGIIK_01575 0.76 -0.5 358,514 + OKGIIK_01575 0.76 -1.5 358,514 + OKGIIK_01575 0.76 -0.6 358,514 + OKGIIK_01575 0.76 -1.8 358,514 + OKGIIK_01575 0.76 -0.5 358,514 + OKGIIK_01575 0.76 -0.3 358,514 + OKGIIK_01575 0.76 -1.9 358,514 + OKGIIK_01575 0.76 -1.3 358,515 - OKGIIK_01575 0.76 -2.7 358,515 - OKGIIK_01575 0.76 -1.3 358,515 - OKGIIK_01575 0.76 -0.0 358,515 - OKGIIK_01575 0.76 -1.0 358,515 - OKGIIK_01575 0.76 +1.0 358,515 - OKGIIK_01575 0.76 -2.2 358,515 - OKGIIK_01575 0.76 -0.1 358,515 - OKGIIK_01575 0.76 -2.6 358,515 - OKGIIK_01575 0.76 -0.5 358,515 - OKGIIK_01575 0.76 -2.8 358,515 - OKGIIK_01575 0.76 -1.5 358,515 - OKGIIK_01575 0.76 +0.0 358,689 + -1.6 358,689 + -0.5 358,715 + +0.2 358,716 - +2.5 358,716 - -3.0 358,729 + -1.8 358,729 + -2.5 358,729 + +1.5 358,729 + +0.5 358,730 - -1.9 358,730 - -1.3 358,730 - -1.7 358,730 - -0.2 358,730 - +1.3 358,730 - -0.3 358,730 - +0.5 358,739 + +0.5
Or see this region's nucleotide sequence