Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt acoB and pdhA are separated by 3 nucleotides pdhA and tDO2 are separated by 238 nucleotides
OKGIIK_00750: acoB - alpha-ketoacid dehydrogenase subunit beta, at 166,496 to 167,476
acoB
OKGIIK_00755: pdhA - pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha, at 167,480 to 168,565
pdhA
OKGIIK_00760: tDO2 - Tryptophan 2,3-dioxygenase (vermilion), at 168,804 to 169,652
tDO2
Position (kb) 167
168
169 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 166.497 kb on + strand at 166.498 kb on - strand at 166.498 kb on - strand at 166.498 kb on - strand at 166.498 kb on - strand at 166.498 kb on - strand at 166.629 kb on + strand, within acoB at 166.948 kb on - strand, within acoB at 167.511 kb on + strand at 167.511 kb on + strand at 167.512 kb on - strand at 167.512 kb on - strand at 167.794 kb on - strand, within pdhA at 168.204 kb on + strand, within pdhA at 168.234 kb on + strand, within pdhA at 168.235 kb on - strand, within pdhA at 168.240 kb on + strand, within pdhA at 168.240 kb on + strand, within pdhA at 168.240 kb on + strand, within pdhA at 168.240 kb on + strand, within pdhA at 168.240 kb on + strand, within pdhA at 168.240 kb on + strand, within pdhA at 168.240 kb on + strand, within pdhA at 168.241 kb on - strand, within pdhA at 168.241 kb on - strand, within pdhA at 168.241 kb on - strand, within pdhA at 168.241 kb on - strand, within pdhA at 168.303 kb on + strand, within pdhA at 168.304 kb on - strand, within pdhA at 168.372 kb on + strand, within pdhA at 168.373 kb on - strand, within pdhA at 168.448 kb on - strand, within pdhA at 168.501 kb on - strand at 168.525 kb on + strand at 168.526 kb on - strand at 168.526 kb on - strand at 168.534 kb on + strand at 168.658 kb on - strand at 168.658 kb on - strand at 168.685 kb on + strand at 168.685 kb on + strand at 168.686 kb on - strand at 168.686 kb on - strand at 168.686 kb on - strand at 168.686 kb on - strand at 168.862 kb on + strand at 168.862 kb on + strand at 168.862 kb on + strand at 168.862 kb on + strand at 168.862 kb on + strand at 168.862 kb on + strand at 168.862 kb on + strand at 168.862 kb on + strand at 168.862 kb on + strand at 168.862 kb on + strand at 168.862 kb on + strand at 168.862 kb on + strand at 168.863 kb on - strand at 168.863 kb on - strand at 168.863 kb on - strand at 168.863 kb on - strand at 168.863 kb on - strand at 168.863 kb on - strand at 168.863 kb on - strand at 168.863 kb on - strand at 168.863 kb on - strand at 168.863 kb on - strand at 168.863 kb on - strand at 168.867 kb on + strand at 168.867 kb on + strand at 168.867 kb on + strand at 168.867 kb on + strand at 168.867 kb on + strand at 168.867 kb on + strand at 168.867 kb on + strand at 168.867 kb on + strand at 168.867 kb on + strand at 168.867 kb on + strand at 168.867 kb on + strand at 168.867 kb on + strand at 168.868 kb on - strand at 168.868 kb on - strand at 168.868 kb on - strand at 168.868 kb on - strand at 168.868 kb on - strand at 168.868 kb on - strand at 168.868 kb on - strand at 168.868 kb on - strand at 168.868 kb on - strand at 168.868 kb on - strand at 168.868 kb on - strand at 168.868 kb on - strand at 168.877 kb on - strand at 169.134 kb on + strand, within tDO2 at 169.134 kb on + strand, within tDO2 at 169.134 kb on + strand, within tDO2 at 169.134 kb on + strand, within tDO2 at 169.134 kb on + strand, within tDO2 at 169.134 kb on + strand, within tDO2 at 169.135 kb on - strand, within tDO2 at 169.135 kb on - strand, within tDO2 at 169.135 kb on - strand, within tDO2 at 169.188 kb on + strand, within tDO2 at 169.302 kb on + strand, within tDO2 at 169.302 kb on + strand, within tDO2 at 169.302 kb on + strand, within tDO2 at 169.302 kb on + strand, within tDO2 at 169.302 kb on + strand, within tDO2 at 169.303 kb on - strand, within tDO2 at 169.303 kb on - strand, within tDO2 at 169.303 kb on - strand, within tDO2 at 169.303 kb on - strand, within tDO2 at 169.320 kb on + strand, within tDO2 at 169.320 kb on + strand, within tDO2 at 169.320 kb on + strand, within tDO2 at 169.320 kb on + strand, within tDO2 at 169.320 kb on + strand, within tDO2 at 169.320 kb on + strand, within tDO2 at 169.321 kb on - strand, within tDO2 at 169.321 kb on - strand, within tDO2 at 169.383 kb on + strand, within tDO2 at 169.425 kb on + strand, within tDO2 at 169.425 kb on + strand, within tDO2 at 169.425 kb on + strand, within tDO2 at 169.425 kb on + strand, within tDO2 at 169.425 kb on + strand, within tDO2 at 169.426 kb on - strand, within tDO2 at 169.426 kb on - strand, within tDO2 at 169.426 kb on - strand, within tDO2 at 169.426 kb on - strand, within tDO2 at 169.426 kb on - strand, within tDO2 at 169.426 kb on - strand, within tDO2 at 169.455 kb on + strand, within tDO2 at 169.455 kb on + strand, within tDO2 at 169.456 kb on - strand, within tDO2 at 169.456 kb on - strand, within tDO2 at 169.456 kb on - strand, within tDO2 at 169.456 kb on - strand, within tDO2 at 169.456 kb on - strand, within tDO2 at 169.456 kb on - strand, within tDO2 at 169.456 kb on - strand, within tDO2 at 169.456 kb on - strand, within tDO2 at 169.456 kb on - strand, within tDO2
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 166,497 + -2.5 166,498 - +1.5 166,498 - -2.2 166,498 - +0.2 166,498 - -0.2 166,498 - -0.0 166,629 + acoB OKGIIK_00750 0.14 -1.8 166,948 - acoB OKGIIK_00750 0.46 -1.0 167,511 + -1.8 167,511 + -1.8 167,512 - -1.7 167,512 - -0.8 167,794 - pdhA OKGIIK_00755 0.29 -1.0 168,204 + pdhA OKGIIK_00755 0.67 -1.9 168,234 + pdhA OKGIIK_00755 0.69 -1.0 168,235 - pdhA OKGIIK_00755 0.70 -1.9 168,240 + pdhA OKGIIK_00755 0.70 -2.2 168,240 + pdhA OKGIIK_00755 0.70 -0.5 168,240 + pdhA OKGIIK_00755 0.70 -1.8 168,240 + pdhA OKGIIK_00755 0.70 +0.5 168,240 + pdhA OKGIIK_00755 0.70 -1.5 168,240 + pdhA OKGIIK_00755 0.70 -1.9 168,240 + pdhA OKGIIK_00755 0.70 -1.0 168,241 - pdhA OKGIIK_00755 0.70 -2.3 168,241 - pdhA OKGIIK_00755 0.70 -0.2 168,241 - pdhA OKGIIK_00755 0.70 -2.6 168,241 - pdhA OKGIIK_00755 0.70 -2.0 168,303 + pdhA OKGIIK_00755 0.76 -2.5 168,304 - pdhA OKGIIK_00755 0.76 -3.0 168,372 + pdhA OKGIIK_00755 0.82 -1.5 168,373 - pdhA OKGIIK_00755 0.82 -0.5 168,448 - pdhA OKGIIK_00755 0.89 -1.8 168,501 - -2.4 168,525 + -1.0 168,526 - -1.9 168,526 - -1.6 168,534 + -1.3 168,658 - -0.5 168,658 - +0.1 168,685 + -2.5 168,685 + +0.5 168,686 - -1.7 168,686 - -0.1 168,686 - -1.0 168,686 - -0.0 168,862 + -2.4 168,862 + -2.6 168,862 + +0.5 168,862 + -0.0 168,862 + -0.0 168,862 + +1.5 168,862 + +2.0 168,862 + +0.2 168,862 + +0.3 168,862 + -1.9 168,862 + -1.3 168,862 + +1.5 168,863 - -1.5 168,863 - -2.2 168,863 - +1.5 168,863 - -0.0 168,863 - -2.7 168,863 - -2.2 168,863 - -0.0 168,863 - -0.6 168,863 - -2.4 168,863 - -1.2 168,863 - -1.5 168,867 + -1.0 168,867 + +1.0 168,867 + +1.1 168,867 + -1.3 168,867 + -1.1 168,867 + -0.3 168,867 + -0.8 168,867 + +0.1 168,867 + -0.2 168,867 + -1.3 168,867 + +2.5 168,867 + -0.8 168,868 - -0.0 168,868 - -2.5 168,868 - -0.6 168,868 - -0.8 168,868 - -1.3 168,868 - -2.0 168,868 - +2.1 168,868 - -0.8 168,868 - -0.2 168,868 - -2.3 168,868 - +0.5 168,868 - -1.9 168,877 - +0.5 169,134 + tDO2 OKGIIK_00760 0.39 -0.0 169,134 + tDO2 OKGIIK_00760 0.39 -0.5 169,134 + tDO2 OKGIIK_00760 0.39 +0.5 169,134 + tDO2 OKGIIK_00760 0.39 -1.8 169,134 + tDO2 OKGIIK_00760 0.39 -0.8 169,134 + tDO2 OKGIIK_00760 0.39 -0.8 169,135 - tDO2 OKGIIK_00760 0.39 -1.0 169,135 - tDO2 OKGIIK_00760 0.39 -1.8 169,135 - tDO2 OKGIIK_00760 0.39 +0.4 169,188 + tDO2 OKGIIK_00760 0.45 -1.3 169,302 + tDO2 OKGIIK_00760 0.59 -0.6 169,302 + tDO2 OKGIIK_00760 0.59 -0.5 169,302 + tDO2 OKGIIK_00760 0.59 -0.1 169,302 + tDO2 OKGIIK_00760 0.59 -1.0 169,302 + tDO2 OKGIIK_00760 0.59 -1.5 169,303 - tDO2 OKGIIK_00760 0.59 -1.8 169,303 - tDO2 OKGIIK_00760 0.59 -0.0 169,303 - tDO2 OKGIIK_00760 0.59 -0.0 169,303 - tDO2 OKGIIK_00760 0.59 -0.0 169,320 + tDO2 OKGIIK_00760 0.61 -0.6 169,320 + tDO2 OKGIIK_00760 0.61 -2.0 169,320 + tDO2 OKGIIK_00760 0.61 -2.8 169,320 + tDO2 OKGIIK_00760 0.61 -0.0 169,320 + tDO2 OKGIIK_00760 0.61 +1.5 169,320 + tDO2 OKGIIK_00760 0.61 +1.5 169,321 - tDO2 OKGIIK_00760 0.61 -0.2 169,321 - tDO2 OKGIIK_00760 0.61 -1.5 169,383 + tDO2 OKGIIK_00760 0.68 +1.5 169,425 + tDO2 OKGIIK_00760 0.73 -1.6 169,425 + tDO2 OKGIIK_00760 0.73 -0.6 169,425 + tDO2 OKGIIK_00760 0.73 -1.3 169,425 + tDO2 OKGIIK_00760 0.73 +0.5 169,425 + tDO2 OKGIIK_00760 0.73 -0.9 169,426 - tDO2 OKGIIK_00760 0.73 -0.5 169,426 - tDO2 OKGIIK_00760 0.73 -2.0 169,426 - tDO2 OKGIIK_00760 0.73 -0.9 169,426 - tDO2 OKGIIK_00760 0.73 -0.5 169,426 - tDO2 OKGIIK_00760 0.73 -0.0 169,426 - tDO2 OKGIIK_00760 0.73 -1.0 169,455 + tDO2 OKGIIK_00760 0.77 -1.4 169,455 + tDO2 OKGIIK_00760 0.77 +0.2 169,456 - tDO2 OKGIIK_00760 0.77 -3.2 169,456 - tDO2 OKGIIK_00760 0.77 +0.9 169,456 - tDO2 OKGIIK_00760 0.77 -1.5 169,456 - tDO2 OKGIIK_00760 0.77 -1.1 169,456 - tDO2 OKGIIK_00760 0.77 -0.3 169,456 - tDO2 OKGIIK_00760 0.77 -2.7 169,456 - tDO2 OKGIIK_00760 0.77 -2.5 169,456 - tDO2 OKGIIK_00760 0.77 -1.5 169,456 - tDO2 OKGIIK_00760 0.77 +0.8
Or see this region's nucleotide sequence