Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_00740 and aceF are separated by 604 nucleotides aceF and acoB are separated by 117 nucleotides
OKGIIK_00740: OKGIIK_00740 - L,D-transpeptidase, at 163,816 to 164,433
_00740
OKGIIK_00745: aceF - branched-chain alpha-keto acid dehydrogenase subunit E2, at 165,038 to 166,378
aceF
OKGIIK_00750: acoB - alpha-ketoacid dehydrogenase subunit beta, at 166,496 to 167,476
acoB
Position (kb) 165
166
167 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 164.161 kb on + strand, within OKGIIK_00740 at 164.161 kb on + strand, within OKGIIK_00740 at 164.161 kb on + strand, within OKGIIK_00740 at 164.161 kb on + strand, within OKGIIK_00740 at 164.161 kb on + strand, within OKGIIK_00740 at 164.161 kb on + strand, within OKGIIK_00740 at 164.161 kb on + strand, within OKGIIK_00740 at 164.161 kb on + strand, within OKGIIK_00740 at 164.161 kb on + strand, within OKGIIK_00740 at 164.162 kb on - strand, within OKGIIK_00740 at 164.162 kb on - strand, within OKGIIK_00740 at 164.162 kb on - strand, within OKGIIK_00740 at 164.162 kb on - strand, within OKGIIK_00740 at 164.162 kb on - strand, within OKGIIK_00740 at 164.162 kb on - strand, within OKGIIK_00740 at 164.162 kb on - strand, within OKGIIK_00740 at 164.162 kb on - strand, within OKGIIK_00740 at 164.174 kb on + strand, within OKGIIK_00740 at 164.174 kb on + strand, within OKGIIK_00740 at 164.174 kb on + strand, within OKGIIK_00740 at 164.174 kb on + strand, within OKGIIK_00740 at 164.175 kb on - strand, within OKGIIK_00740 at 164.175 kb on - strand, within OKGIIK_00740 at 164.175 kb on - strand, within OKGIIK_00740 at 164.175 kb on - strand, within OKGIIK_00740 at 164.263 kb on + strand, within OKGIIK_00740 at 164.263 kb on + strand, within OKGIIK_00740 at 164.263 kb on + strand, within OKGIIK_00740 at 164.263 kb on + strand, within OKGIIK_00740 at 164.263 kb on + strand, within OKGIIK_00740 at 164.263 kb on + strand, within OKGIIK_00740 at 164.264 kb on - strand, within OKGIIK_00740 at 164.264 kb on - strand, within OKGIIK_00740 at 164.264 kb on - strand, within OKGIIK_00740 at 164.264 kb on - strand, within OKGIIK_00740 at 164.264 kb on - strand, within OKGIIK_00740 at 164.264 kb on - strand, within OKGIIK_00740 at 164.264 kb on - strand, within OKGIIK_00740 at 164.264 kb on - strand, within OKGIIK_00740 at 164.264 kb on - strand, within OKGIIK_00740 at 164.264 kb on - strand, within OKGIIK_00740 at 164.264 kb on - strand, within OKGIIK_00740 at 164.293 kb on + strand, within OKGIIK_00740 at 164.294 kb on - strand, within OKGIIK_00740 at 164.341 kb on + strand, within OKGIIK_00740 at 164.341 kb on + strand, within OKGIIK_00740 at 164.341 kb on + strand, within OKGIIK_00740 at 164.341 kb on + strand, within OKGIIK_00740 at 164.341 kb on + strand, within OKGIIK_00740 at 164.341 kb on + strand, within OKGIIK_00740 at 164.342 kb on - strand, within OKGIIK_00740 at 164.342 kb on - strand, within OKGIIK_00740 at 164.342 kb on - strand, within OKGIIK_00740 at 164.342 kb on - strand, within OKGIIK_00740 at 164.342 kb on - strand, within OKGIIK_00740 at 164.342 kb on - strand, within OKGIIK_00740 at 164.386 kb on + strand at 164.386 kb on + strand at 164.386 kb on + strand at 164.386 kb on + strand at 164.387 kb on - strand at 164.387 kb on - strand at 164.387 kb on - strand at 164.387 kb on - strand at 164.403 kb on + strand at 164.403 kb on + strand at 164.404 kb on - strand at 164.404 kb on - strand at 164.404 kb on - strand at 164.404 kb on - strand at 164.404 kb on - strand at 164.431 kb on + strand at 164.431 kb on + strand at 164.431 kb on + strand at 164.432 kb on - strand at 164.432 kb on - strand at 164.432 kb on - strand at 164.620 kb on + strand at 164.620 kb on + strand at 164.620 kb on + strand at 164.620 kb on + strand at 164.620 kb on + strand at 164.620 kb on + strand at 164.620 kb on + strand at 164.620 kb on + strand at 164.620 kb on + strand at 164.620 kb on + strand at 164.620 kb on + strand at 164.620 kb on + strand at 164.620 kb on + strand at 164.621 kb on - strand at 164.621 kb on - strand at 164.621 kb on - strand at 164.621 kb on - strand at 164.621 kb on - strand at 164.621 kb on - strand at 164.621 kb on - strand at 164.621 kb on - strand at 164.946 kb on + strand at 164.946 kb on + strand at 164.946 kb on + strand at 164.946 kb on + strand at 164.947 kb on - strand at 164.980 kb on + strand at 164.980 kb on + strand at 164.980 kb on + strand at 164.980 kb on + strand at 164.980 kb on + strand at 164.980 kb on + strand at 164.981 kb on - strand at 164.981 kb on - strand at 164.981 kb on - strand at 164.981 kb on - strand at 165.280 kb on + strand, within aceF at 165.281 kb on - strand, within aceF at 165.281 kb on - strand, within aceF at 165.283 kb on - strand, within aceF at 165.926 kb on + strand, within aceF at 166.218 kb on + strand, within aceF at 166.219 kb on - strand, within aceF at 166.356 kb on + strand at 166.357 kb on - strand at 166.398 kb on - strand at 166.398 kb on - strand at 166.398 kb on - strand at 166.398 kb on - strand at 166.398 kb on - strand at 166.398 kb on - strand at 166.451 kb on + strand at 166.451 kb on + strand at 166.475 kb on + strand at 166.475 kb on + strand at 166.476 kb on - strand at 166.476 kb on - strand at 166.476 kb on - strand at 166.476 kb on - strand at 166.476 kb on - strand at 166.476 kb on - strand at 166.476 kb on - strand at 166.476 kb on - strand at 166.476 kb on - strand at 166.497 kb on + strand at 166.498 kb on - strand at 166.498 kb on - strand at 166.498 kb on - strand at 166.498 kb on - strand at 166.498 kb on - strand at 166.629 kb on + strand, within acoB at 166.948 kb on - strand, within acoB
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 164,161 + OKGIIK_00740 0.56 -0.8 164,161 + OKGIIK_00740 0.56 -1.8 164,161 + OKGIIK_00740 0.56 +1.5 164,161 + OKGIIK_00740 0.56 -1.8 164,161 + OKGIIK_00740 0.56 -0.8 164,161 + OKGIIK_00740 0.56 -1.5 164,161 + OKGIIK_00740 0.56 -2.5 164,161 + OKGIIK_00740 0.56 +0.7 164,161 + OKGIIK_00740 0.56 -0.8 164,162 - OKGIIK_00740 0.56 +0.8 164,162 - OKGIIK_00740 0.56 -0.1 164,162 - OKGIIK_00740 0.56 -0.2 164,162 - OKGIIK_00740 0.56 -0.0 164,162 - OKGIIK_00740 0.56 -1.0 164,162 - OKGIIK_00740 0.56 -0.5 164,162 - OKGIIK_00740 0.56 +0.7 164,162 - OKGIIK_00740 0.56 -0.5 164,174 + OKGIIK_00740 0.58 -1.8 164,174 + OKGIIK_00740 0.58 +1.5 164,174 + OKGIIK_00740 0.58 -0.8 164,174 + OKGIIK_00740 0.58 +1.5 164,175 - OKGIIK_00740 0.58 -1.1 164,175 - OKGIIK_00740 0.58 -1.3 164,175 - OKGIIK_00740 0.58 +0.5 164,175 - OKGIIK_00740 0.58 -0.3 164,263 + OKGIIK_00740 0.72 -0.0 164,263 + OKGIIK_00740 0.72 -0.5 164,263 + OKGIIK_00740 0.72 -1.8 164,263 + OKGIIK_00740 0.72 -1.2 164,263 + OKGIIK_00740 0.72 -0.5 164,263 + OKGIIK_00740 0.72 -1.3 164,264 - OKGIIK_00740 0.72 -1.5 164,264 - OKGIIK_00740 0.72 -0.6 164,264 - OKGIIK_00740 0.72 -0.7 164,264 - OKGIIK_00740 0.72 +0.2 164,264 - OKGIIK_00740 0.72 -0.3 164,264 - OKGIIK_00740 0.72 -1.5 164,264 - OKGIIK_00740 0.72 -0.5 164,264 - OKGIIK_00740 0.72 -1.3 164,264 - OKGIIK_00740 0.72 -0.6 164,264 - OKGIIK_00740 0.72 -1.8 164,264 - OKGIIK_00740 0.72 +0.5 164,293 + OKGIIK_00740 0.77 +0.7 164,294 - OKGIIK_00740 0.77 -2.3 164,341 + OKGIIK_00740 0.85 -1.0 164,341 + OKGIIK_00740 0.85 -0.0 164,341 + OKGIIK_00740 0.85 +2.5 164,341 + OKGIIK_00740 0.85 -0.8 164,341 + OKGIIK_00740 0.85 -1.9 164,341 + OKGIIK_00740 0.85 -0.5 164,342 - OKGIIK_00740 0.85 -1.6 164,342 - OKGIIK_00740 0.85 -2.0 164,342 - OKGIIK_00740 0.85 +0.1 164,342 - OKGIIK_00740 0.85 -0.3 164,342 - OKGIIK_00740 0.85 +1.3 164,342 - OKGIIK_00740 0.85 -2.5 164,386 + +0.1 164,386 + -0.5 164,386 + -2.7 164,386 + -1.9 164,387 - -0.0 164,387 - -1.0 164,387 - +0.7 164,387 - -2.5 164,403 + -1.0 164,403 + -0.8 164,404 - -0.5 164,404 - +1.5 164,404 - -2.0 164,404 - +1.5 164,404 - +2.1 164,431 + -2.3 164,431 + +0.1 164,431 + -1.3 164,432 - -2.7 164,432 - -0.5 164,432 - +0.5 164,620 + -2.3 164,620 + -1.5 164,620 + -2.9 164,620 + -0.0 164,620 + -0.9 164,620 + +0.3 164,620 + -2.9 164,620 + +0.3 164,620 + -1.8 164,620 + -0.8 164,620 + +1.5 164,620 + -0.8 164,620 + -0.8 164,621 - -2.2 164,621 - -1.0 164,621 - -0.8 164,621 - -1.6 164,621 - -0.8 164,621 - -0.5 164,621 - -2.7 164,621 - -1.8 164,946 + -1.0 164,946 + -2.0 164,946 + -1.3 164,946 + +1.0 164,947 - -1.5 164,980 + -1.2 164,980 + +0.3 164,980 + +1.0 164,980 + -1.0 164,980 + -1.3 164,980 + +1.0 164,981 - -0.5 164,981 - -0.0 164,981 - +1.8 164,981 - -2.7 165,280 + aceF OKGIIK_00745 0.18 -1.8 165,281 - aceF OKGIIK_00745 0.18 -4.6 165,281 - aceF OKGIIK_00745 0.18 -0.8 165,283 - aceF OKGIIK_00745 0.18 -2.4 165,926 + aceF OKGIIK_00745 0.66 -0.5 166,218 + aceF OKGIIK_00745 0.88 -1.0 166,219 - aceF OKGIIK_00745 0.88 -1.3 166,356 + -1.5 166,357 - -1.6 166,398 - -2.8 166,398 - -1.3 166,398 - -0.0 166,398 - -1.0 166,398 - -1.0 166,398 - +1.5 166,451 + -2.3 166,451 + -1.0 166,475 + -0.5 166,475 + -3.5 166,476 - -0.8 166,476 - +0.3 166,476 - -1.2 166,476 - -0.9 166,476 - +0.1 166,476 - -1.2 166,476 - -0.5 166,476 - -1.5 166,476 - -0.9 166,497 + -2.5 166,498 - +1.5 166,498 - -2.2 166,498 - +0.2 166,498 - -0.2 166,498 - -0.0 166,629 + acoB OKGIIK_00750 0.14 -1.8 166,948 - acoB OKGIIK_00750 0.46 -1.0
Or see this region's nucleotide sequence