Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tsaA and OKGIIK_00410 are separated by 58 nucleotides OKGIIK_00410 and OKGIIK_00415 are separated by 2 nucleotides OKGIIK_00415 and OKGIIK_00420 are separated by 28 nucleotides OKGIIK_00420 and OKGIIK_00425 are separated by 160 nucleotides
OKGIIK_00405: tsaA - tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO, at 98,029 to 98,604
tsaA
OKGIIK_00410: OKGIIK_00410 - YCII domain-containing protein, at 98,663 to 99,037
_00410
OKGIIK_00415: OKGIIK_00415 - hypothetical protein, at 99,040 to 99,561
_00415
OKGIIK_00420: OKGIIK_00420 - haloacid dehalogenase, at 99,590 to 100,306
_00420
OKGIIK_00425: OKGIIK_00425 - hypothetical protein, at 100,467 to 100,721
_00425
Position (kb) 99
100 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 98.162 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.172 kb on - strand, within tsaA at 98.172 kb on - strand, within tsaA at 98.172 kb on - strand, within tsaA at 98.172 kb on - strand, within tsaA at 98.172 kb on - strand, within tsaA at 98.172 kb on - strand, within tsaA at 98.172 kb on - strand, within tsaA at 98.172 kb on - strand, within tsaA at 98.528 kb on + strand, within tsaA at 98.528 kb on + strand, within tsaA at 98.528 kb on + strand, within tsaA at 98.529 kb on - strand, within tsaA at 98.577 kb on + strand at 98.577 kb on + strand at 98.577 kb on + strand at 98.577 kb on + strand at 98.577 kb on + strand at 98.577 kb on + strand at 98.578 kb on - strand at 98.578 kb on - strand at 98.578 kb on - strand at 98.578 kb on - strand at 98.578 kb on - strand at 98.578 kb on - strand at 98.578 kb on - strand at 98.606 kb on - strand at 98.606 kb on - strand at 98.684 kb on + strand at 98.684 kb on + strand at 98.684 kb on + strand at 98.684 kb on + strand at 98.684 kb on + strand at 98.684 kb on + strand at 98.684 kb on + strand at 98.684 kb on + strand at 98.685 kb on - strand at 98.685 kb on - strand at 98.685 kb on - strand at 98.685 kb on - strand at 98.685 kb on - strand at 98.685 kb on - strand at 98.685 kb on - strand at 98.685 kb on - strand at 98.891 kb on + strand, within OKGIIK_00410 at 98.891 kb on + strand, within OKGIIK_00410 at 98.891 kb on + strand, within OKGIIK_00410 at 98.891 kb on + strand, within OKGIIK_00410 at 98.891 kb on + strand, within OKGIIK_00410 at 98.891 kb on + strand, within OKGIIK_00410 at 98.891 kb on + strand, within OKGIIK_00410 at 98.892 kb on - strand, within OKGIIK_00410 at 98.892 kb on - strand, within OKGIIK_00410 at 98.892 kb on - strand, within OKGIIK_00410 at 98.892 kb on - strand, within OKGIIK_00410 at 98.892 kb on - strand, within OKGIIK_00410 at 99.404 kb on - strand, within OKGIIK_00415 at 99.404 kb on - strand, within OKGIIK_00415 at 99.427 kb on + strand, within OKGIIK_00415 at 99.428 kb on - strand, within OKGIIK_00415 at 99.644 kb on + strand at 99.644 kb on + strand at 99.644 kb on + strand at 99.645 kb on - strand at 99.645 kb on - strand at 99.645 kb on - strand at 99.645 kb on - strand at 99.647 kb on + strand at 99.689 kb on + strand, within OKGIIK_00420 at 99.689 kb on + strand, within OKGIIK_00420 at 99.689 kb on + strand, within OKGIIK_00420 at 99.689 kb on + strand, within OKGIIK_00420 at 99.690 kb on - strand, within OKGIIK_00420 at 99.690 kb on - strand, within OKGIIK_00420 at 99.690 kb on - strand, within OKGIIK_00420 at 99.938 kb on + strand, within OKGIIK_00420 at 99.938 kb on + strand, within OKGIIK_00420 at 99.938 kb on + strand, within OKGIIK_00420 at 99.938 kb on + strand, within OKGIIK_00420 at 99.938 kb on + strand, within OKGIIK_00420 at 99.938 kb on + strand, within OKGIIK_00420 at 99.938 kb on + strand, within OKGIIK_00420 at 99.938 kb on + strand, within OKGIIK_00420 at 99.938 kb on + strand, within OKGIIK_00420 at 99.939 kb on - strand, within OKGIIK_00420 at 99.939 kb on - strand, within OKGIIK_00420 at 99.939 kb on - strand, within OKGIIK_00420 at 99.939 kb on - strand, within OKGIIK_00420 at 99.939 kb on - strand, within OKGIIK_00420 at 99.939 kb on - strand, within OKGIIK_00420 at 99.939 kb on - strand, within OKGIIK_00420 at 99.939 kb on - strand, within OKGIIK_00420 at 99.939 kb on - strand, within OKGIIK_00420 at 99.939 kb on - strand, within OKGIIK_00420 at 100.058 kb on + strand, within OKGIIK_00420 at 100.058 kb on + strand, within OKGIIK_00420 at 100.059 kb on - strand, within OKGIIK_00420 at 100.178 kb on + strand, within OKGIIK_00420 at 100.179 kb on - strand, within OKGIIK_00420 at 100.179 kb on - strand, within OKGIIK_00420 at 100.304 kb on + strand at 100.304 kb on + strand at 100.305 kb on - strand at 100.305 kb on - strand at 100.319 kb on + strand at 100.319 kb on + strand at 100.319 kb on + strand at 100.320 kb on - strand at 100.320 kb on - strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.381 kb on + strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.382 kb on - strand at 100.453 kb on + strand at 100.468 kb on + strand at 100.468 kb on + strand at 100.468 kb on + strand at 100.468 kb on + strand at 100.468 kb on + strand at 100.468 kb on + strand at 100.468 kb on + strand at 100.468 kb on + strand at 100.468 kb on + strand at 100.468 kb on + strand at 100.468 kb on + strand at 100.468 kb on + strand at 100.469 kb on - strand at 100.469 kb on - strand at 100.469 kb on - strand at 100.469 kb on - strand at 100.486 kb on + strand at 100.486 kb on + strand at 100.486 kb on + strand at 100.486 kb on + strand at 100.486 kb on + strand at 100.486 kb on + strand at 100.487 kb on - strand at 100.487 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 98,162 + tsaA OKGIIK_00405 0.23 -0.3 98,171 + tsaA OKGIIK_00405 0.25 -2.5 98,171 + tsaA OKGIIK_00405 0.25 -0.8 98,171 + tsaA OKGIIK_00405 0.25 -2.9 98,171 + tsaA OKGIIK_00405 0.25 +0.5 98,171 + tsaA OKGIIK_00405 0.25 -1.9 98,171 + tsaA OKGIIK_00405 0.25 -2.5 98,171 + tsaA OKGIIK_00405 0.25 +0.1 98,171 + tsaA OKGIIK_00405 0.25 +0.8 98,171 + tsaA OKGIIK_00405 0.25 -2.2 98,171 + tsaA OKGIIK_00405 0.25 -2.2 98,171 + tsaA OKGIIK_00405 0.25 +0.8 98,171 + tsaA OKGIIK_00405 0.25 -1.9 98,171 + tsaA OKGIIK_00405 0.25 -2.3 98,171 + tsaA OKGIIK_00405 0.25 -0.5 98,172 - tsaA OKGIIK_00405 0.25 -0.9 98,172 - tsaA OKGIIK_00405 0.25 -2.9 98,172 - tsaA OKGIIK_00405 0.25 -0.5 98,172 - tsaA OKGIIK_00405 0.25 -1.5 98,172 - tsaA OKGIIK_00405 0.25 -0.9 98,172 - tsaA OKGIIK_00405 0.25 +0.5 98,172 - tsaA OKGIIK_00405 0.25 -0.9 98,172 - tsaA OKGIIK_00405 0.25 -2.4 98,528 + tsaA OKGIIK_00405 0.87 -0.5 98,528 + tsaA OKGIIK_00405 0.87 -0.6 98,528 + tsaA OKGIIK_00405 0.87 +2.1 98,529 - tsaA OKGIIK_00405 0.87 -2.0 98,577 + +0.5 98,577 + -0.0 98,577 + -1.5 98,577 + -1.5 98,577 + -0.6 98,577 + -0.8 98,578 - -1.9 98,578 - +1.5 98,578 - -0.8 98,578 - -2.3 98,578 - +0.2 98,578 - +0.1 98,578 - -1.8 98,606 - +0.0 98,606 - -1.3 98,684 + -1.3 98,684 + -0.8 98,684 + -0.5 98,684 + +0.7 98,684 + +1.5 98,684 + -0.9 98,684 + -0.3 98,684 + -0.4 98,685 - -0.8 98,685 - +0.1 98,685 - -0.6 98,685 - -0.8 98,685 - -0.8 98,685 - -1.8 98,685 - -0.7 98,685 - -1.9 98,891 + OKGIIK_00410 0.61 -0.5 98,891 + OKGIIK_00410 0.61 +1.9 98,891 + OKGIIK_00410 0.61 -2.2 98,891 + OKGIIK_00410 0.61 +1.1 98,891 + OKGIIK_00410 0.61 +1.5 98,891 + OKGIIK_00410 0.61 -0.0 98,891 + OKGIIK_00410 0.61 -0.6 98,892 - OKGIIK_00410 0.61 -1.3 98,892 - OKGIIK_00410 0.61 -0.8 98,892 - OKGIIK_00410 0.61 -2.5 98,892 - OKGIIK_00410 0.61 -0.3 98,892 - OKGIIK_00410 0.61 -1.0 99,404 - OKGIIK_00415 0.70 -2.8 99,404 - OKGIIK_00415 0.70 -2.8 99,427 + OKGIIK_00415 0.74 -0.9 99,428 - OKGIIK_00415 0.74 -1.9 99,644 + -1.0 99,644 + +2.0 99,644 + -2.2 99,645 - -3.8 99,645 - -1.8 99,645 - -1.5 99,645 - -0.3 99,647 + -0.0 99,689 + OKGIIK_00420 0.14 -1.3 99,689 + OKGIIK_00420 0.14 -1.1 99,689 + OKGIIK_00420 0.14 -1.6 99,689 + OKGIIK_00420 0.14 +0.5 99,690 - OKGIIK_00420 0.14 -0.8 99,690 - OKGIIK_00420 0.14 -1.0 99,690 - OKGIIK_00420 0.14 +1.0 99,938 + OKGIIK_00420 0.49 -1.0 99,938 + OKGIIK_00420 0.49 -2.2 99,938 + OKGIIK_00420 0.49 +1.1 99,938 + OKGIIK_00420 0.49 -1.5 99,938 + OKGIIK_00420 0.49 +1.5 99,938 + OKGIIK_00420 0.49 +1.5 99,938 + OKGIIK_00420 0.49 -1.8 99,938 + OKGIIK_00420 0.49 -1.8 99,938 + OKGIIK_00420 0.49 -1.0 99,939 - OKGIIK_00420 0.49 -1.8 99,939 - OKGIIK_00420 0.49 -2.0 99,939 - OKGIIK_00420 0.49 +0.2 99,939 - OKGIIK_00420 0.49 -1.8 99,939 - OKGIIK_00420 0.49 +1.5 99,939 - OKGIIK_00420 0.49 -1.4 99,939 - OKGIIK_00420 0.49 -1.5 99,939 - OKGIIK_00420 0.49 -1.6 99,939 - OKGIIK_00420 0.49 +1.5 99,939 - OKGIIK_00420 0.49 +0.1 100,058 + OKGIIK_00420 0.65 -1.3 100,058 + OKGIIK_00420 0.65 -0.8 100,059 - OKGIIK_00420 0.65 -0.6 100,178 + OKGIIK_00420 0.82 +0.4 100,179 - OKGIIK_00420 0.82 +0.7 100,179 - OKGIIK_00420 0.82 -1.2 100,304 + -1.9 100,304 + -0.5 100,305 - +1.0 100,305 - -1.5 100,319 + -0.3 100,319 + -1.5 100,319 + -0.8 100,320 - -0.5 100,320 - -1.6 100,381 + -2.6 100,381 + -1.8 100,381 + -1.9 100,381 + -0.7 100,381 + -2.0 100,381 + -1.5 100,381 + -1.1 100,381 + -1.8 100,381 + -1.5 100,381 + -1.7 100,381 + +1.5 100,381 + -0.5 100,381 + -1.3 100,381 + -2.3 100,381 + -1.0 100,382 - -0.5 100,382 - -1.8 100,382 - +1.2 100,382 - -3.0 100,382 - -2.5 100,382 - -0.0 100,382 - -0.0 100,382 - -0.9 100,382 - -0.6 100,382 - +1.5 100,382 - +0.5 100,382 - -1.3 100,382 - -1.9 100,382 - -0.0 100,382 - -1.0 100,382 - -0.8 100,382 - -0.8 100,453 + -0.1 100,468 + -1.2 100,468 + -1.0 100,468 + -0.3 100,468 + -1.5 100,468 + +2.5 100,468 + -0.3 100,468 + +0.3 100,468 + -0.5 100,468 + -2.9 100,468 + -1.9 100,468 + -2.1 100,468 + -2.6 100,469 - -1.4 100,469 - -1.6 100,469 - -0.0 100,469 - +0.5 100,486 + -1.9 100,486 + -2.7 100,486 + -0.5 100,486 + +1.1 100,486 + -2.3 100,486 + -2.3 100,487 - -1.0 100,487 - -3.2
Or see this region's nucleotide sequence