Strain Fitness in Escherichia coli ECRC101 around MCAODC_23905

Experiment: Control_ECRC101

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcadC1 and MCAODC_23900 are separated by 4 nucleotidesMCAODC_23900 and eAL are separated by 101 nucleotideseAL and MCAODC_23910 are separated by 97 nucleotidesMCAODC_23910 and MCAODC_23915 overlap by 4 nucleotides MCAODC_23895: cadC1 - transcriptional regulator, at 1,502,800 to 1,503,612 cadC1 MCAODC_23900: MCAODC_23900 - FidL, at 1,503,617 to 1,504,108 _23900 MCAODC_23905: eAL - EAL domain, c-di-GMP-specific phosphodiesterase class I (or its enzymatically inactive variant), at 1,504,210 to 1,505,820 eAL MCAODC_23910: MCAODC_23910 - GGDEF domain-containing protein, at 1,505,918 to 1,506,334 _23910 MCAODC_23915: MCAODC_23915 - Putative diguanylate cyclase, at 1,506,331 to 1,507,275 _23915 Position (kb) 1504 1505 1506Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1503.251 kb on + strand, within cadC1at 1504.264 kb on + strandat 1504.566 kb on + strand, within eALat 1505.305 kb on + strand, within eALat 1505.616 kb on + strand, within eALat 1505.690 kb on - strandat 1505.772 kb on - strandat 1505.797 kb on - strandat 1505.988 kb on + strand, within MCAODC_23910at 1506.086 kb on - strand, within MCAODC_23910at 1506.231 kb on - strand, within MCAODC_23910at 1506.464 kb on - strand, within MCAODC_23915

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC101
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1,503,251 + cadC1 MCAODC_23895 0.55 -0.5
1,504,264 + -1.4
1,504,566 + eAL MCAODC_23905 0.22 +0.6
1,505,305 + eAL MCAODC_23905 0.68 +3.2
1,505,616 + eAL MCAODC_23905 0.87 +0.2
1,505,690 - -1.9
1,505,772 - -0.8
1,505,797 - -2.1
1,505,988 + MCAODC_23910 0.17 -0.1
1,506,086 - MCAODC_23910 0.40 -2.1
1,506,231 - MCAODC_23910 0.75 -0.4
1,506,464 - MCAODC_23915 0.14 +1.1

Or see this region's nucleotide sequence