Strain Fitness in Escherichia coli ECRC101 around MCAODC_21970

Experiment: Control_ECRC101

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntminD and minE are separated by 3 nucleotidesminE and MCAODC_21970 are separated by 371 nucleotidesMCAODC_21970 and tnp-IS3 are separated by 83 nucleotidestnp-IS3 and tnp-IS3 overlap by 1 nucleotides MCAODC_21960: minD - septum site-determining protein MinD, at 1,173,832 to 1,174,644 minD MCAODC_21965: minE - cell division topological specificity factor MinE, at 1,174,648 to 1,174,914 minE MCAODC_21970: MCAODC_21970 - autotransporter outer membrane beta-barrel domain-containing protein, at 1,175,286 to 1,175,996 _21970 MCAODC_21975: tnp-IS3 - IS3 family transposase, at 1,176,080 to 1,176,967 tnp-IS3 MCAODC_21980: tnp-IS3 - IS3 family transposase, at 1,176,967 to 1,177,293 tnp-IS3 Position (kb) 1175 1176Strain fitness (log2 ratio) -2 -1 0 1 2at 1174.912 kb on + strandat 1174.923 kb on + strandat 1174.935 kb on + strandat 1175.349 kb on - strandat 1175.351 kb on - strandat 1175.579 kb on - strand, within MCAODC_21970at 1175.579 kb on - strand, within MCAODC_21970at 1175.581 kb on - strand, within MCAODC_21970at 1175.581 kb on - strand, within MCAODC_21970at 1175.581 kb on - strand, within MCAODC_21970at 1175.688 kb on - strand, within MCAODC_21970at 1176.070 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC101
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1,174,912 + +0.7
1,174,923 + -0.3
1,174,935 + +1.0
1,175,349 - +1.2
1,175,351 - -1.4
1,175,579 - MCAODC_21970 0.41 +1.3
1,175,579 - MCAODC_21970 0.41 +0.9
1,175,581 - MCAODC_21970 0.41 +0.3
1,175,581 - MCAODC_21970 0.41 +0.7
1,175,581 - MCAODC_21970 0.41 -2.3
1,175,688 - MCAODC_21970 0.57 +1.8
1,176,070 - -0.5

Or see this region's nucleotide sequence